CURRICULUM VITAE
Hiroshi Kimura
E-mail: hkimura@hmro.kyoto-u.ac.jp
Work address:
Nuclear Function and Dynamics Unit,
HMRO, Graduate School of Medicine,
Kyoto University,
Yoshida Konoe-cho, Sakyo-ku,
Kyoto 606-8501,
Japan
Home address:
42-23 Kitagosho-cho, Okazaki
Sakyo-ku,
Kyoto 606-8336,
Japan
Tel: +81 75 753 9289
Fax: +81 75 753 9281
Tel: +81 75 752 5157
Fax: +81 75 752 5157
Date of birth: 12th March 1965
Nationality: Japanese
Languages: Japanese, English
Marital status: Married with two children (6 and 12 years-old)
Qualification
Mar. 1996
Doctor of Science (DSc): (Chemistry II), Hokkaido University
Thesis title: Study of mouse MCM proteins - are they DNA
replication licensing factors?
Apr. 1989 – Oct. 1991
DSc course: (Chemistry II), Hokkaido University
Project title: Isolation of autonomous replication sequences
from human cells. (Supervisor: Prof. Kazunori Sugimoto)
Apr. 1987 – Mar. 1989
MA: (Science, Chemistry II), Hokkaido University
Apr. 1983 – Mar. 1987
BA: (Science, Chemistry II), Hokkaido University
Employment
Aug. 2003 – present
Professor (temporally-and-specially-assigned)
Horizontal Medical Research Organization
Graduate School of Medicine
Kyoto University
Sakyo-ku, Kyoto 606-8501, Japan
Feb. 2002 – July 2003
Associate Professor
Department of Functional Genomics
Medical Research Institute
Tokyo Medical and Dental University
Bunkyo-ku, Tokyo 113-8510, Japan
Department Head: Prof. Masatoshi Hagiwara
May 1996 – Feb. 2002
Postdoctoral Research Associate
Sir William Dunn School of Pathology
University of Oxford
Oxford, UK
Laboratory: Prof. Peter R. Cook
Nov. 1991 – Apr. 1996
Research Associate
Research Center for Molecular Genetics
Hokkaido University
Sapporo, Japan
Directors: Profs. Kazunori Sugimoto and Nobuo Takagi
Fellowships and Grants
2004 – 2008
‘Molecular mechanisms of chromatin assembly and dynamics’
Grant-in-aid for Scientific Research on Priority Area ‘Nuclear
Dynamics’ from the Ministry of Education, Science, Sports and Culture
(MEXT) of Japan
2004 – 2006
‘Human transcription regulome by genome tiling array’
Genome Network Project, Japan Science and Technology Agency
(co-applicant; coordinated by Dr. Katsuhiko Shirahige, Tokyo Institute
of Technology)
2004 – 2005
‘An in vitro transcription system based on permeabilized cells’
Grant-in-aid for Exploratory Research from the MEXT of Japan
2003 – 2004
‘Histone dynamics and gene expression in living cells’
Grant-in-aid for Basic Scientific Research (B) from the MEXT of Japan
1996 – 1998
‘Purification of DNA replication factories’
Long-term Fellowship from Human Frontier Science Program,
Strasbourg, France
1995 – 1996
‘Function of mouse Mcm proteins on DNA replication’
Grant-in-aid for Scientific Research on Priority Area ‘Nuclear Structure
and Function’ from the MEXT of Japan
1995 – 1996
‘Phosphorylational regulation of mouse Mcm3 protein’
Grant-in-aid for Encouragement of Young Scientists from the Mext of
Japan
1994 – 1995
‘Function of mouse Mcm proteins’
Grant-in-aid for Scientific Research on Priority Area ‘Molecular
biology of the regulation of DNA replication’ from MEXT of Japan
Teaching experience
2003 – present
Instructor/Lecturer for Fluorescence Microscopy Courses supported by
21st century COE program at Hokkaido and Osaka Universities
2003 – present
Temporal Lecturer on Biochemistry and Molecular Biology for
undergraduate students at Tokyo Medical and Dental University
2002 – 2003
Lecturer on Biochemistry and Molecular Biology for undergraduate
and graduate students at Tokyo Medical and Dental University
2000 – 2002
Tutor for undergraduate students at Sir William Dunn School of
Pathology, University of Oxford
1999 – 2002
Instructor for Cell Biology Practical Course for undergraduate students
at Sir William Dunn School of Pathology, University of Oxford
1991 – 1996
Instructor/Lecturer for Recombinant DNA Technical Courses at
Research Center for Molecular Genetics, Hokkaido University
Publication list
Original articles
Kimura, H., Takizawa, N., Allemand, E., Hori, T., Iborra, F.J., Nozaki, N., Muraki, M.,
Hagiwara, M., Krainer, A.R., Fukagawa, T. and Okawa, K. (2006). A novel histoneexchange factor, protein phosphatase 2C, mediates the exchange and dephosphorylation of
H2A-H2B. J. Cell Biol. 175, 389-400.
Kitamura, A., Kubota, H., Pack, C.G., Matsumoto, G., Hirayama, S., Takahashi, Y., Kimura,
H., Kinjo, M., Morimoto, R.I. and Nagata, K. (2006). Cytosolic chaperonin prevents
polyglutamine toxicity with altering aggregation state. Nature Cell Biol. 8, 1163-1169.
Minoshima, Y., Hori, T., Okada, M., Kimura, H., Haraguchi, T., Hiraoka, Y., Bao, Y.C.,
Kawashima, T., Kitamura, T. and Fukagawa, T. (2005). The Constitutive centromere
component CENP-50 is required for recovery from spindle damage. Mol. Cell. Biol. 25,
10315-10328.
Mikami, Y., Hori, T., Kimura, H. and Fukagawa, T. (2005). The functional region of CENPH interacts with the Nuf2 complex that localizes to centromere during mitosis. Mol. Cell.
Biol. 25, 1958-1970.
Muraki, M., Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K.,
Yomoda, J., Murray, M.V., Kimura, H., Furuichi, K., Shibuya, H., Krainer, A.R., Suzuki, M.
and Hagiwara, M. (2004). Manipulation of alternative splicing by a newly developed
inhibitor of Clks. J. Biol. Chem. 279, 24246-24254.
Iborra, F.J., Kimura H. and Cook P.R. (2004). The functional organization of mitochondrial
genomes in human cells. BMC Biol. 2, 9.
Inoue, K., Zama, T., Kamimoto, T., Aoki, R., Ikeda, Y., Kimura, H. and Hagiwara, M.
(2004). TNFalpha-induced ATF3 expression is bidirectionally regulated by the JNK and
ERK pathways in vascular endothelial cells. Genes Cells 9, 59-70.
Tatsumi, Y., Ohta, S., Kimura, H., Tsurimoto, T. and Obuse, C. (2003). The ORC1 cycle in
human cells: I. Cell cycle-regulated oscillation of human ORC1. J. Biol. Chem. 278, 4152841534.
Duringer, C., Hamiche, A., Gustafsson, L., Kimura, H. and Svanborg, C. (2003). HAMLET
interacts with histones and chromatin in tumor cell nuclei. J .Biol. Chem. 278, 42131-42135.
Hori, T., Haraguchi, T., Hiraoka, Y., Kimura, H. and Fukagawa, T. (2003). Dynamic
behavior of Nuf2-Hec1 complex that localizes to centrosome and centromere and is essential
for mitotic progression in vertebrate cells. J. Cell Sci. 116, 3347-3362.
Suzuki, H., Wu, J., Hossain, K., Ohhata, T., Du, J., Akhand, A.A., Hayakawa, A., Kimura,
H., Hagiwara, M. and Nakashima, I. (2003). Involvement of MKK6 in TCRαβintCD69lo: a
target population for apoptotic cell death in thymocytes. FASEB J. 17, 1538-1540.
Ishimi, Y., Okayasu, I., Kato, C., Kwon, H.J., Kimura, H., Yamada, K. and Song, S.Y.
(2003). Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix.
Eur. J. Biochem. 279, 1089-1101.
Kudo, Y., Boyd, C.A.R., Kimura, H., Cook, P.R., Redman, C.W.G. and Sargent, I.L. (2003).
Quantifying the syncytialisation of human placental trophoblast BeWo cells grown in vitro.
Biochim. Biophys. Acta. 1640, 25-31.
Kimura, H., Sugaya, K. and Cook, P.R. (2002). The transcription cycle of RNA polymerase
II in living cells. J. Cell Biol. 159, 777-782.
Kwek, K.Y., Murphy, S., Furger, A., Thomas, B., O’Gorman, W., Kimura, H., Proudfoot,
N.J. and Akoulitchev, A. (2002). U1 snRNA associates with TFIIH and regulates
transcriptional initiation. Nature Struct. Biol. 9, 800-805.
Takebayashi, S.I., Manders, E.M.M., Kimura, H., Taguchi, H. and Okumura, K. (2001).
Mapping sites where replication initiates in mammalian cells using DNA fibers. Exp. Cell
Res. 271, 263-268.
Kimura, H. and Cook, P.R. (2001). Kinetics of core histones in living human cells: little
exchange of H3 and H4 and some rapid exchange of H2B. J. Cell Biol. 153, 1341-1353.
Wade-Martins, R., White, R.E., Kimura, H., Cook, P.R. and James, M.R. (2000). Stable
correction of a genetic deficiency in human cells by an episome carrying a 115 kb genomic
transgene. Nature Biotech. 18, 1311-1314.
Kuwahara, K., Yoshida, M., Kondo, E., Sakata, A., Watanabe, Y., Abe, E., Kouno, Y.,
Tomiyasu, S., Fujimura, S., Tokuhisa, T., Kimura, H., Ezaki, T. and Sakaguchi., N. (2000).
A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal
center and associated with MCM3, a protein essential for DNA replication. Blood 95, 23212328.
Jones, E., Kimura, H., Vigneron, M., Wang, Z., Roeder, R.G. and Cook, P.R. (2000).
Isolation and characterization of monoclonal antibodies directed against subunits of human
RNA polymerases I, II, and III. Exp. Cell Res. 254, 163-172.
Kimura, H., Tao, Y., Roeder, R.G. and Cook, P.R. (1999). Quantitation of RNA polymerase
II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant
amounts of polymerases attached to the nuclear substructure. Mol. Cell. Biol. 19, 5383-5392.
Manders, E.M., Kimura, H. and Cook, P.R. (1999). Direct imaging of DNA in living cells
reveals the dynamics of chromosome formation. J. Cell Biol. 144, 813-821.
Ishimi, Y., Komamura, Y., You, Z. and Kimura, H. (1998). Biochemical function of mouse
minichromosome maintenance 2 protein. J. Biol. Chem. 273, 8369-8375.
Kimura, H., Ohtomo, T., Yamaguchi, M., Ishii, A. and Sugimoto, K. (1996). Mouse MCM
proteins: complex formation and transportation to the nucleus. Genes Cells 1, 977-993.
Ishimi, Y., Ichinose, S., Omori, A., Sato, K. and Kimura, H. (1996). Binding of human
minichromosome maintenance proteins with histone H3. J. Biol. Chem. 271, 24115-24122.
Yoshida, I., Kimura, H. and Takagi, N. (1996). The mouse Mcmd gene for DNA replication
protein P1MCM3 maps to bands A3-A5 on chromosome 1 by fluorescence in situ
hybridization. Genomics 32, 483-484.
Takizawa, N., Kimura, H. and Sugimoto, K. (1995). Sequence of mouse CDC47, a member
of the minichromosome maintenance (Mcm) family involved in the DNA replication
licensing system. Gene 167, 343-344.
Kimura, H., Takizawa, N., Nozaki, N. and Sugimoto, K. (1995). Molecular cloning of cDNA
encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical
interaction between P1(MCM3) and CDC46 proteins. Nucl. Acids Res. 23, 2097-2104.
Kimura, H., Nozaki, N. and Sugimoto, K. (1994). DNA polymerase  associated protein P1,
a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA
synthetic period. EMBO J. 13, 4311-4320.
Review articles
Kimura, H. (2005). Histone dynamics in living cells revealed by photobleaching. DNA
Repair 4, 939-950.
Kimura, H. and Cook, P.R. (2003). Methods for visualizing histone exchange in living cells.
Methods Enzymol. 375, 381-93.
Pombo, A., Jones, E., Iborra, F.J., Kimura, H., Sugaya, K., Cook, P.R., and Jackson, D.A.
(2000). Specialized transcription factories within mammalian nuclei. Crit. Rev. Eukaryot.
Gene Expr. 10, 21-29.
Book Chapter Contribution
Kimura, H. and Cook, P.R. (2006). Structure and function of inner-nuclear architectures.
Nuclear Dynamics (ed. K. Takeyasu and K. Nagata), Springer-Verlag Tokyo, Tokyo. (in
press)
Oral presentation at international symposia/meetings
Hiroshi Kimura: A novel link between histone deposition and dephosphorylation through a
type 2C protein phosphatase.
The 21st Century COE Symposium ‘Chromatin Signaling’ (Nov. 2-3, 2006; Osaka,
Japan), Invited
Hiroshi Kimura, Nanako Takizawa, Michiko Muraki and Katsuya Okawa: Histone H2A/H2B
exchange factors identified by a novel in vitro reconstitution system using permeabilized
cells.
The 2nd Alan Wolffe EMBO Conference on Chromatin and Epigenetics (May 19-22,
2005; Heidelberg, Germany), Selected
Hiroshi Kimura: Reconstitution of histone exchange in vitro.
The 21st Radiation Biology Center International Symposium on Chromatin and
Epigenetic Memory in Damage Response (Oct. 27-29, 2004; Kyoto, Japan), Invited
Hiroshi Kimura: Histone dynamics: from visualization to reconstitution.
The 5th International Conference supported by MEXT Japan: Nano and Visual
Biology of Chromosome Dynamics (Oct. 16-17, 2004; Kyoto, Japan), Invited
Hiroshi Kimura: Histone dynamics and gene expression in living cells.
The 6th International Congress of International Federation of Associations of
Anatomists (Aug. 22-27, 2004; Kyoto, Japan), Invited
Hiroshi Kimura: Kinetics of histones in living human cells: Roles of H2A variants and factors
that regulate histone exchange.
The 1st Pacific-rim International Conference on Protein Science (Apr. 14-18, 2004;
Yokohama, Japan), Invited
Hiroshi Kimura: Kinetics of chromatin proteins in living cells revealed by long-term FRAP
(fluorescence recovery after photobleaching).
Joint A-IMBN/EMBO course: Fluorescence Microscopy of Living Cells (17-23
November, 2002; Kobe, Japan), Invited
Hiroshi Kimura: Kinetics of chromatin transcription in living cells revealed by FLIP and
FRAP.
Symposium ‘4D imaging in living cells’ (Nov. 27, 2002; Leiden, The Netherlands),
Invited
Referees
Prof. Peter R. Cook
Sir William Dunn School of Pathology
University of Oxford
South Parks Road
Oxford OX1 3RE
UK
Phone: +44 1865 275 528
Fax: +44 1865 275 515
E-mail: peter.cook@path.ox.ac.uk
Dr. Tom Misteli
National Cancer Institute
National Institutes of Health
9000 Rockville Pike
Bethesda, MA 20892
USA.
Phone: +1 301 402 3959
Fax: +1 928 832 0970
Email: mistelit@mail.nih.gov
Prof. Haruhiko Takisawa
Department of Biological Sciences
Graduate School of Science
Osaka University
1-1 Machikaneyama-cho
Toyonaka, Osaka 560-0043
JAPAN
Phone: +81 6 6850 6762/5554
Fax: +81 6 6850 6762/5554
takisawa@bio.sci.osaka-u.ac.jp