Sample of Style of the Manuscript-Animal Science Journal
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Page 1: The Title Page
The title page, Abstract and key words, text, acknowledgments, references and figure legends should be
made using Microsoft-Word Version 97 ~ 2003 (extension: .doc).
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Title: Basic characterization of heat shock protein 70 genes HSPA2 and HSPA8 from the
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Japanese quail, Coturnix japonica
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Authors:
Kei
HANZAWA,1
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TAWEETUNGTRAGOON3
Takashi
SHIINA2
and
Autthaporn
S.
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Institute, address, country: 1Department of Animal Science, Tokyo University of
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Agriculture, Atsugi, 2Department of Molecular Life Science, Tokai University School of
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Medicine, Kanagawa, Japan; and 3Gibthai Company Limited, Bangkok, Thailand
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Running Head: HSP70-FAMILY GENES IN THE JAPANESE QUAIL
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Correspondence: Kei Hanzawa, Department of Animal Science, Tokyo University of
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Agriculture, Atsugi, Kanagawa 243-0034, Japan.
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Phone: +81-46-270-6567; Facsimile: +81-46-27-6569; Email: khanzawa@nodai.ac.jp
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Title: Only head of the title and proper nouns should be written in capital letters and no period should be put at
the end.
Authors: The first and last names of each author should be written in full. A middle name should be written as
the initials followed by a period.
Institute, address, country: Department and University / Research organization, City / Prefecture and area,
and Country should be given. They should be written in italics. Numbers should connect the institute with
each author.
Running Head (Short Title): It should be written within 40 characters. Nouns should be written as a capital
letter and no period should be put at the end.
Correspondence: Name, institute, address, ZIP code, country, and facsimile number, e-mail address should be
given.
Sample of Style of the Manuscript-Animal Science Journal
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Page 2: ABSTRACT & Key words
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ABSTRACT
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We have identified two members of the heat shock protein (HSP) 70 family from the
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Japanese quail, Coturnix japonica. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
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- - - - - - - - - - - - - - - - - - - - - - -. Taken together, the data suggest that CjHSPA2 and
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CjHSPA8 encode orthologs of HSPA2 and HSPA8 with the notable difference that
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CjHSPA2 and CjHSPA8 are heat shock-inducible genes, whereas HSPA2 and HSPA8
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are constitutively expressed in a heat shock-independent manner.
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Key words: gene structure, heat shock protein 70, HSPA2, HSPA8, Japanese quail.
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ABSTRACT: should be written within 200 words or less on page 2.
Up to five Key words and/or phrases (consisting of less than five words) can be written at the end of the
abstract, and arranged in alphabetical order.
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Sample of Style of the Manuscript-Animal Science Journal
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Page 3 and following pages: text, acknowledgements, references, figure
legends, figure(s) and table(s)
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INTRODUCTION
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Proteins in the heat shock protein 70 family - - - - - - - - - - - - many proteins (Hartl 1996). -
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- - - - - - - - - - - - . The cytoplasm (CY) and endoplasmic reticula (ER) groups are - - - - - -
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(Drori & Loosli 1960). Seven CY genes have been identified in the human genome (Drori
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et al. 1959a). - - - - - - - - - - - --. Morimoto et al. (1986) already reported in - - - - - - -
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chickens (Gallus gallus, or Gg).
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CD1 molecules play an important role in the immune system, presenting
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lipid-contain - - - - - - (Vincent et al. 2003; Brigl & Brenner 2004). CD1 transcription is
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strong in chicken lymphoid tissues, particularly bursa and spleen (Drori et al. 1959b;
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Morimoto et al. 1986; Miller M et al. unpublished. data, 2008). - - - - .
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Reference citation in the text: References in text should be written as surname of the first author and
published year in parenthesis. The author's initial should not be included.
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If there are two authors use ‘and’: Smith and Jones (2001); but if cited within parentheses use ‘&’: (Smith &
Jones).
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If the number of authors of one reference is more than three, only the last name of first author should be
indicated and add “et al.”
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Plural references in a place should be divided by semicolons and written in chronological order.
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*If references could not be distinguished by the above-mentioned rule of citation (Author(s) and published
year), put the alphabet behind the published year depending on the order that appeared in the text.
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Reference to unpublished data and personal communications should not appear in the list but should be cited in
the text only.
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Sample of Style of the Manuscript-Animal Science Journal
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MATERIALS AND METHODS
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Animal care and nucleic acid isolation
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The management of Japanese quail and all procedures in the present study were
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performed according to the Animal Experimental Guidelines for Tokyo University of
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Agriculture. - - - - - - - - - - - - - - - - - - - - -
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Amplification of full-length cDNA sequences for three quail HSP70 genes
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Complete cDNA sequences for CjHSPA2 and CjHSPA8 were amplified using - - - - - - - - -
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---------
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RESULTS
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Isolation and sequence of quail HSP70 family cDNA
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We identified cDNA sequences that - - - - - - - - - - - - - -. Therefore, mammals and avian
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belong to different clusters, with the exception that, at the amino acid level, CjHSPA8 is
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more similar to HsHSPA8 than it is to GgHSC70 (Fig. 1). - - - - - - - Figure 2 shows the
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results of -----.
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Accordingly, the conceptual translations of the quail HSP70 genes show a high level
of sequence identity to chicken and human proteins.
[Figure 1]
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A place to insert Figure and/or Table should appoint in text.
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Sample of Style of the Manuscript-Animal Science Journal
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DISCUSSION
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CjHSPA2 expression is elevated in response to heat shock treatment
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We next asked if any of the quail HSP70-related genes reported here respond to heat
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shock treatment. - - - - - -
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Conclusion
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Our knowledge regarding avian MHC has increased
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Do not make a CONCLUSION section. The study conclusion should be stated at the end of the
DISCUSSION.
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ACKNOWLEDGMENTS
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This work was supported by Grant-in-Aid for Scientific Research from ----If the work is supported by any grants, ACKNOWLEDMENTS should be written.
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Sample of Style of the Manuscript-Animal Science Journal
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REFERENCES
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Drori D, Loosli JK. 1960. - - - - - - - - - - - - - - - -
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Drori D, Loosli JK, Corbett JC. 1959b. Influence of fistulation on the digestibility of feeds
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by steers. Journal of Animal Science 18, 206-210.
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Drori D, Loosli JK, Smith JA. 1959a. - - - - - - - - - - - - - - - - -
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Drori D, Walter L. 1955. - - - - - - - - - - - - - - - - -
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Folley SJ, Malpress FH. 1948. Hormonal control of mammary growth. In: Pincuss G,
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Thimann KV (eds), The Hormones, Vol.1, pp. 695-743. Academic Press, New York.
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Harlt FU. 1996. - - - - - - - - - - - - - - - - -
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Morimoto RR, Hunt A, Berg CA. 1986. - - - - - - - - - - - - - - - - -
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Nalbandov AV. 1963. Advances in Neuroendocrinology 2nd edn. University of Illinois
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Press, Urbana, IL.
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National Center for Biotechnology Information (NCBI). 1990. Nucleotide-nucleotide
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BLAST (blastn) [homepage on the Internet]. National Center for Biotechnology
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Information, Bethesda, MD; [cited 13 December 2002]. Available from URL:
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http://www.ncbi.nlm.nih.gov/blast/
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All the cited papers in the text should be listed in REFERENCES.
All the papers in REFERENCES should be cited in the text.
The Harvard (author, date) system of referencing is used.
References should be listed in alphabetical order of the first author's surname. Plural references by the
same author should be listed by the chronological order. Plural references by the same author published in the
same year should be listed in alphabetical order of the last name of second author.
References from ‘journals’ should be written as: Name of authors (do not add “and” just before the last
author), publication year, title, journal name with full spelling, volume, and first and last pages, should be
given. Period should not be put at the initial of author’s name. The issue number in a volume should be
omitted.
Reference from ‘books’ should be written as: Name of authors, published year, book name, edition, pp.,
publisher and published place should be given. In case of a chapter in a book, “In: title of the chapter and
name of editor(s)” should be given.
Reference from ‘electric material’ should be written as: Publisher, published year, name of web site, name of
authors, published place, cited date and URL address should be given.
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Sample of Style of the Manuscript-Animal Science Journal
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Figure legends
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Figure 1
Morphological profiled on erythroid cells from equine peripheral blood
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during incubation in a two phase liquid culture system.
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erythroblasts, M Ebl: mature erythroblasts, D Ery: denucleated erythroid cells. Values
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are expressed as mean ± SE.
Im Ebl: immature
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Figure 2
Changes in percentage of haemoglobin containing cells on erythroid cells
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from equine peripheral blood during incubation in a two phase liquid culture system.
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Im Ebl: immature erythroblasts, M Ebl: mature erythroblasts, D Ery: denucleated
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erythroid cells. Values are expressed as mean ± SE.
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Legends should be self-explanatory and typed on separate pages.
The legend should incorporate definitions of any symbols used and all abbreviations and
units of measurement should be explained so that the figure and its legend are
understandable without reference to the text.
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Sample of Style of the Manuscript-Animal Science Journal
Examples for Figure and Table (Prepare on separate pages from the text)
Fig. 1
Graphics should be supplied as high resolution (at least 300 d.p.i.) files, saved as .tif, .jpg, .eps, or Power
Point 97‾2003 (.ppt) format.
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Sample of Style of the Manuscript-Animal Science Journal
Table 1 Changes in percentage of blood group antigen expressed cells on erythroid cells
from equine peripheral blood during incubation in a two phase liquid culture system
Blood System
A
C
D
Factor
Aa
Ca
Dk
Dl
Days of culture
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0
0
0
0
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0
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10.5 ± 3.3a
7.1 ± 3.3a
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19.9 ± 3.3ab
17.2 ± 5.1ab
5.3 ± 5.1a
3.2 ± 2.5a
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37.7 ± 2.4b
31.8 ± 3.9b
15.4 ± 4.2a
12.5 ± 2.3a
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56.4 ± 4.1c
54.5 ± 2.4c
21.1 ± 1.4ab
18.0 ± 3.5ab
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91.5 ± 1.5d
78.3 ± 4.6d
52.2 ± 2.1c
51.0 ± 5.4c
Values are expressed as mean ± SE. Different letters indicate significant differences (a <
b < c < d, P < 0.05).
Tables should be made using Microsoft-Excel Version 97 ~ 2003 (extension: .xls).
The style and format of the table should be according to the instructions for authors and the recent issues.
Abbreviations used in a table must be defined in that table.
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