Liyun Liu
CURRICULUM VITAE
10/12/06
Liyun Liu, Ph.D.
Department of Biology
California State University
18111 Nordhoff Street
Northridge, CA 91330-8303
Tel: (818) 677-2754
Fax: (818) 677-2034
LIYUN.LIU@CSUN.EDU
Website: www.csun.edu/~ll656883/index.html
PROFESIONAL POSITIONS
2006-present:
Assistant Professor
Department of Biology
California State University
Northridge, California
2004-2006:
Research Associate
Department of Molecular and Cellular Biology
Harvard University
Cambridge, Massachusetts
1999-2004:
Postdoctoral Fellow
Department of Molecular and Cellular Biology
Harvard University
Advisor: Professor J. WOODLAND HASTINGS
Research Area: Biochemistry, protein crystallography and molecular biology of
bioluminescence in dinoflagellates
EDUCATION
1991-1999: Ph.D. in Evolutionary and Environmental Biology
Department of Biology, University of Louisiana, Lafayette, Louisiana
Advisor: Professor THOMAS C. PESACRETA
Dissertation Title: Myosin cDNA sequences of corn (Zea mays, Panicoideae): expression
in tissues and phylogenetic implications.
1990-1991: Graduate Program in Biology
Department of Biology, University of Maryland, College Park, Maryland
Advisors: Professors STEVEN HUTCHESON and DAVID STRANEY
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Liyun Liu
CURRICULUM VITAE
10/12/06
1986-1990: Doctoral Degree of Natural Sciences
Institute of Botany, Chinese Academy of Sciences, Beijing, China
Advisor: Professor FU-XIONG WANG
Project: Cytological and biochemical Studies of Self-incompatibility in Brassica napus L.
1983-1986: M.S. in Botany, Nankai University, Tianjin, China
1979-1983: B.S. in Biology, Hunan Teacher’s College, Changsha, China
TEACHING EXPERIENCES
Fall semester 2006, Instructor, California State University, Northridge, Courses taught:
BIOL 572: Recombinant DNA Technique
BIOL 572L: Recombinant DNA Technique LAB
2000-2005, Department of Molecular and Cellular Biology, Harvard University
Supervision of ten undergraduates in laboratory research
1993-1999, Teaching Assistant, Microscopy Center, University of Louisiana, Lafayette
Course taught: Optical and Electron Microscopy in Biological Sciences
RESEARCH EXPERTIES and INTERESTS
2006-present, California State University, Northridge
Genomics and proteomics of dinoflagellates and malaria parasites
1999-2006, Harvard University
Molecular cloning and bioinformatic analysis of luciferase genes of dinoflagellates
Biochemical characterization and mutagenesis of recombinant dinoflagellate luciferases
Expression of dinoflagellate luciferases in bacteria for X-ray protein crystallography
Crystallization of bacuolovirus-expressed dinoflagellate luciferin-binding protein
Microarray analysis of cell density-dependent gene expression in yeast
Study of functions of the FLT-3 kinase and MAPKAP2 kinase in leukemia
1993-1999, the University of Louisiana
Isolation of myosin genes from corn and onion
Production of plant myosins for generating antibodies in rabbits
Purification of myosins from onion by chromatography
Immunolocalization of myosins in plant cells by light and electron microscopy
PUBLICATIONS
1) FULL-LENGTH ARTICLES
11. Liu, L. and Hastings, J.W. Two different domains of the luciferase gene in the heterotrophic
dinoflagellate Noctiluca scintillons occur as two separate genes in photosynthetic species.
Proc. Natl. Acad. Sci. USA (in press).
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Liyun Liu
CURRICULUM VITAE
10/12/06
10. Liu, L. and Hastings, J. W. (2006). Novel and rapidly diverging intergenic sequences between
tandem repeats of the luciferase genes in seven dinoflagellate species. J. Phycology 42: 96103.
9. Hastings, J. W., Liu, L., and Schultz, W. (2005). Dinoflagellate bioluminescence and its
circadian regulation. In Bioluminescence (John Lee, Editor), the Digital Photobiology
Compendium (Dennis P. Valenzeno, Editor).
8. Liu, L., Schultz, L. W., and Hastings, J. W. (2005). pH regulation of luciferase activity in
dinoflagellates involves a novel enzymatic mechanism. In Bioluminescence &
Chemiluminescence: (A. Tsuji, M. Maeda, L.J. Kricka, and P. E. Stanley, Eds.). p.15-19.
World Scientific Publ., London.
7. Schultz, L. W., Liu, L., Cegielski, M., and Hastings, J. W. (2005). Crystal structure of a pHregulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole. Proc.
Natl. Acad. Sci. USA 102: 1378-1383.
6. Yang, X., Liu, L., Sterngberg, D., Tang, L., Galinsky, I., DeAngelo, D., and Stone, R. (2005).
The FLT3 internal tandem duplication mutation prevents apoptosis in interleukin-3-deprived
BaF3 cells due to protein kinase A and ribosomal S6 kinase 1–mediated BAD
phosphorylation at Serine 112. Cancer Research 65: 7338-7347.
5. Liu, L., Wilson, T., and Hastings, J. W. (2004). Molecular evolution of dinoflagellate
luciferases, enzymes with three catalytic domains in a single polypeptide. Proc. Natl. Acad.
Sci. USA 101: 16555-16560.
4. Liu, L., Im, H., Cegielski, M., LeMagueres, P., Schultz, L. W., Krause, K. L., and Hastings, J.
W. (2003). Characterization and crystallization of active domains of a novel luciferase from a
marine dinoflagellate. Acta Crystallogr. D 59: 761-764.
3. Okamoto, O. K., Liu, L., Robertson, D. L., and Hastings, J. W. (2001). Members of a
dinoflagellate luciferase gene family differ in synonymous substitution rates. Biochemistry
40: 15862-15868.
2. Li, L., Liu, L*., Hong, R., Robertson, D. L., and Hastings, J. W. (2001). N-terminal
intramolecularly conserved histidines of three domains in Gonyaulax luciferase are
responsible for loss of activity in the alkaline region. Biochemistry 40: 1844-1849. (* joint
first author).
1. Liu, L., Zhou, J., and Pesacreta, T. C. (2001). Maize myosins: diversity, localization, and
function. Cell Motility and the Cytoskeleton 48: 130-148.
2) ABSTRACTS AND MEETING REPORTS
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Liyun Liu
CURRICULUM VITAE
10/12/06
9. Liu, L. and Hastings J.W. (2004). Phylogenetic analysis of dinoflagellate luciferase genes from
seven species: a possible role for conserved nucleotides in the circadian regulation of protein
synthesis. Bioluminescence & Chemiluminescence: 12th International Symposium, Yokahama,
Japan, supplement.
8. Schultz, W., Cegielski, M., Liu, L., and Hastings, J.W. (2004). Structural studies of a luciferase
from the marine dinoflagellate, Lingulodinium polyedrum. Abstracts of the 32nd Annual Meeting
of American Society for Photobiology, Seattle, WA.
7. Krause K, Le Mageres1, P., Im, H., Schultz, W., Hastings, J.W., and Liu, L. (2003). Threedimensional structure analysis of the D2 domain of Lingulodinium luciferase. Abstracts of the
31st Annual Meeting of American Society for Photobiology, Baltimore, MD.
6. Hastings, J.W., Liu, L., Krause, K., Im, H., and Schultz, W. (2002). Structural studies of the
catalytic domains from a dinoflagellate luciferase. Abstracts of the 30th Annual Meeting of
American Society for Photobiology. Quebec City, Canada.
5. Okamoto O.K., Liu, L., Robertson, D., and Hastings, J.W. (2001). Molecular structure of the
luciferase gene from the dinoflagellate Pyrocystis lunula. Abstracts of the 29th Annual Meeting
of American Society for Photobiology, Chicago, IL. p. 34.
4. Liu, L. and Hastings, J.W. (2001). Structural requirements for enzymatic activity in a single
catalytic domain of a dinoflagellate luciferase. Abstracts of the 29th Annual Meeting of American
Society for Photobiology, Chicago, IL. p. 31.
3. Liu, L., Robertson, D., and Hastings, J.W. (2000). N-terminal histidines are responsible for the
decrease in Gonyaulax luciferase activity at pH 8.0. Bioluminescence & Chemiluminescence: 11th
International Symposium, Asilomar, CA. p. 60.
2. Liu, L. and Pesacreta, T.C. (1997). Multiple myosins from maize. Plant Physiology 114: 855.
1. Liu, L. and Pesacreta, T.C. (1994). Diversity of monocotyledon myosin genes. Molecular
Biology of the Cell (Suppl.) 5: 277a
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