Lê Đức Thảo1, Nguyễn Viết Dũng1, Lê Huy Hàm1 ABSTRACT The results of gerbera breeding by gamma irradiation in vitro Four gerbera varieties DT1, DT2, DT3, DT4 are widely grown in different flower production areas of Vietnam. However, there are some negative charateristics which have to be improved. With this aim and to diversify the current Gerbera varieties, Agricultural Genetics Institute used gamma irradiation (60Co) on callus of 4 above mentioned gerbera varieties with radiation doses 0 (original varieties), 5, 10, 20, 30Gy. To evaluate the initial results, 114 beneficial mutation lines have been obtained with the morphologies such as: Low stem, change color of petals, change color of stamens and small leaf angle. After observing, evaluating and comparing 114 mutation lines and original varieties by standard indicators of biological agriculture in the fields, 12 promising lines have been selected. Key words: Gerbera, mutation, irradiation, variance, breeding