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UNIVERSITY OF OSLO
Faculty of Mathematics and Natural Sciences
Exam in MBV4250/ MBV9250 Basic immunology and immunological techniques
Day of exam: Friday March 30
Exam hours: 15.00 – 18.00
This examination paper consists of 2 page.
Appendices:None
Permitted materials: None
Make sure that your copy of this examination paperis complete before answering.
The adaptive immune response begins when antigen presenting cells display antigenic peptides to
naive helper T cells. The peptides are displayed on surface major histocompatibility complex (MHC)
class II molecules.
A)
B)
C)
D)
How and where are proteins processed, before peptides are loaded on MHC class II?
How are the peptides selected for loading?
What is the role of invariant chain?
The actual presentation happens in lymph nodes. Describe briefly lymph node anatomy, and
the molecular mechanisms that regulate cell migration to the lymph nodes.
Helper T cells that recognise the peptide antigen/MHC complex become activated and gain the ability
to regulate other cells.
E)
F)
G)
H)
I)
Give a detailed description of the interaction between peptide/MHC and the T cell receptor.
What is the role of CD3 and CD4/8?
How would you use FLOW cytometry to characterize a T cell response?
What are the characteristics of regulatory T cells, regarding surface molecule expression and
function ?
How would you use FLOW cytometry to analyze and sort regulatory T cells?
The enzyme AID is important for two important processes in B cells, namely somatic hypermutation
and class swith recombination.
J) Describe AID regarding expression pattern and function.
K) If you have cloned the V genes from a number of B cells and would like to know whether or
not the B cells have been through somatic hypermutation, how would you answer the
question?