Genome Discovery Core - Vanderbilt University

advertisement
Genome Technology Core
Pilot Project Application
Vanderbilt University Medical Center has invested in a next-generation DNA sequencing
platform (Illumina Genome Analyzer) that will be implemented this spring. This new instrument
has the capability of producing up to a gigabase of DNA sequence per run and offers a broad
range of scientific applications including microbial genome re-sequencing, targeted gene resequencing, whole transcriptome analysis, and chromatin immunoprecipitation followed by
sequencing (ChIP-Seq). This new resource will greatly expand scientific opportunities for many
investigators and should enhance competitiveness for extramural funding.
This sequencing technology will be available through a newly created shared resource called
the Genome Technology Core (GTC) that will be operated jointly by three existing core
facilities: the Vanderbilt Microarray Shared Resource, the Vanderbilt DNA Sequencing Facility,
and the Computational Genomics Core in the Center for Human Genetics Research. We are
now seeking applications for pilot projects that will be utilized for early access to this technology.
Because this instrument represents a substantial investment by the Medical Center, faculty in
the School of Medicine will have priority access but we wish to encourage pilot project
applications from faculty from the entire campus. Pilot projects will be evaluated based on
several criteria including feasibility, scientific impact and anticipated benefits for obtaining
extramural funding. The costs of these new services will be determined after the instrument has
been implemented and protocols adopted.
Deadline for submission of pilot projects is Friday April 4, 2008. Applications are to be
submitted via e-mail to Christie Ingram, christie.ingram@vanderbilt.edu. Questions may also be
directed to Ms. Ingram.
Genome Technology Core
Pilot Project Application
The information provided in this pilot project application will be used to help determine how the
GTC resource may be used to help achieve your project’s objectives and to maximize
experiment success.
A. Applicant Information
1. Name:
2. Campus Address:
3. Campus Phone:
4. E-mail:
B. Service Requested
1. Whole-genome re-sequencing
2. Targeted gene re-sequencing
3. ChIP-Seq
4. Whole transcriptome analysis (digital gene expression profiling)
a. Tag profiling
b. Small RNA discovery and analysis
C. Project Information - Please limit to 1 page.
1. Project title:
2. PI:
3. Funding source(s) available (i.e. grant number or cost center):
4. Specific aims and a brief description of how this service will help achieve goals
5. Project description (<150 words)
a. Include preliminary data that supports the objectives (optional)
7. Impact on specific opportunities for new extramural funding
8. Deadlines for obtaining data that will impact grant submissions or publications
D. Sequencing Template
Experiment success is directly related to template quality. Information provided will be
used to evaluate likelihood of experiment success.
1. Description (genomic, amplified, mitochondrial, BAC, RNA, etc):
2. Source:
3. Concentration:
4. Extraction method:
5. Complexity (i.e. size of target genome, transcriptome, or aggregate)
6. Availability - Is the template currently available and ready to be submitted? If not, when
will the template be ready for submission?
7. Have templates produced successful results in another assay? Please briefly describe.
8. Other information:
Download