Genome Technology Core Pilot Project Application Vanderbilt University Medical Center has invested in a next-generation DNA sequencing platform (Illumina Genome Analyzer) that will be implemented this spring. This new instrument has the capability of producing up to a gigabase of DNA sequence per run and offers a broad range of scientific applications including microbial genome re-sequencing, targeted gene resequencing, whole transcriptome analysis, and chromatin immunoprecipitation followed by sequencing (ChIP-Seq). This new resource will greatly expand scientific opportunities for many investigators and should enhance competitiveness for extramural funding. This sequencing technology will be available through a newly created shared resource called the Genome Technology Core (GTC) that will be operated jointly by three existing core facilities: the Vanderbilt Microarray Shared Resource, the Vanderbilt DNA Sequencing Facility, and the Computational Genomics Core in the Center for Human Genetics Research. We are now seeking applications for pilot projects that will be utilized for early access to this technology. Because this instrument represents a substantial investment by the Medical Center, faculty in the School of Medicine will have priority access but we wish to encourage pilot project applications from faculty from the entire campus. Pilot projects will be evaluated based on several criteria including feasibility, scientific impact and anticipated benefits for obtaining extramural funding. The costs of these new services will be determined after the instrument has been implemented and protocols adopted. Deadline for submission of pilot projects is Friday April 4, 2008. Applications are to be submitted via e-mail to Christie Ingram, christie.ingram@vanderbilt.edu. Questions may also be directed to Ms. Ingram. Genome Technology Core Pilot Project Application The information provided in this pilot project application will be used to help determine how the GTC resource may be used to help achieve your project’s objectives and to maximize experiment success. A. Applicant Information 1. Name: 2. Campus Address: 3. Campus Phone: 4. E-mail: B. Service Requested 1. Whole-genome re-sequencing 2. Targeted gene re-sequencing 3. ChIP-Seq 4. Whole transcriptome analysis (digital gene expression profiling) a. Tag profiling b. Small RNA discovery and analysis C. Project Information - Please limit to 1 page. 1. Project title: 2. PI: 3. Funding source(s) available (i.e. grant number or cost center): 4. Specific aims and a brief description of how this service will help achieve goals 5. Project description (<150 words) a. Include preliminary data that supports the objectives (optional) 7. Impact on specific opportunities for new extramural funding 8. Deadlines for obtaining data that will impact grant submissions or publications D. Sequencing Template Experiment success is directly related to template quality. Information provided will be used to evaluate likelihood of experiment success. 1. Description (genomic, amplified, mitochondrial, BAC, RNA, etc): 2. Source: 3. Concentration: 4. Extraction method: 5. Complexity (i.e. size of target genome, transcriptome, or aggregate) 6. Availability - Is the template currently available and ready to be submitted? If not, when will the template be ready for submission? 7. Have templates produced successful results in another assay? Please briefly describe. 8. Other information: