Questions on Bacterial Genomics Chapter 10 ) Which of these is another term for the Sanger method of DNA sequencing? a) chemical degradation method b) dideoxy sequencing. c) restriction method d) label method e) gel method 4) What is an open-reading frame? a) A non-translated region on the chromosome. b) an unknown gene. c) A gene that no longer has a function. d) The protein encoding sequence of a gene. e) The intron region of a gene. 6) What is a transcriptome? a) The coding region on the chromosome. b) The coding region on the chromosome minus the introns. c) The coding region on the chromosome plus the control regions. d) The transcripts encoded for by the genes within a genome. e) The mount of mRNA in the cell. 8) Microarray analysis may be used to study: a) gene expression in cells grown under different conditions. b) protein expression in cells grown under different conditions. c) rate of chromosomal replication in cells. d) enzyme activity in cells. e) growth rate of cells when grown under different conditions. 13) Term for genes from different organisms that encode for proteins that carry out the same function but have different sequences. a) homologs b) paralogs c) orthologs d) metalogs e) semilogs 16) What is metagenomics? a) A culture-dependent method that may be used to determine genome sequences. b) A culture-dependent method that may be used to find new enzymes. c) A culture-dependent method that may be used to study groups of microbes in an ecosystem. d) A culture-independent method that may be used to document microbial community composition. e) A culture –independent method that may be used for the isolation of different organisms.