Table S2. Diversity statistics, segregation analysis statistics and functional annotations of the 97 genic-SSRs in C.lanceolata
Heterozygositya
SSR locus
Na
χ 2 test of
BLASTX top hit
(NCBI accession)
Ho
He
PIC
Segregationb
BLASTX
Putative function at E≤10− 5 [organism]
E-value
FeSSR7
2
0.375
0.325
0.258
Normal
-
No significant match
-
CFeSSR8
2
0.25
0.233
0.195
Invariant
XP_003593177.1
Chloroplast chlorophyll a/b-binding protein CP24 [Medicago truncatula]
9.00E-119
CFeSSR10
3
0.5
0.667
0.563
Invariant
NP_974447.1
alpha-galactosidase [Arabidopsis thaliana]
2.00E-17
CFeSSR12
2
0.5
0.4
0.305
Normal
NP_197710.1
CYP86B1; electron carrier [Arabidopsis thaliana]
5.00E-99
CFeSSR16
2
0.25
0.4
0.305
Normal
ABL97952.1
DNA binding transcription factor [Brassica rapa]
4.00E-58
CFeSSR20
3
0.625
0.658
0.551
Normal
XP_002511688.1
O-sialoglycoprotein endopeptidase, putative [Ricinus communis]
6.00E-17
CFeSSR23
2
0.375
0.325
0.258
Normal
dbj|BAC21275.1|
lachrymatory factor synthase [Allium cepa]
5.00E-10
CFeSSR29
2
0.875
0.525
0.371
Normal
XP_002532019.1
No significant match
-
CFeSSR35
2
0.125
0.125
0.11
Invariant
XP_002303496.1
f-box family protein [Populus trichocarpa]
4.00E-26
CFeSSR36
3
0.5
0.633
0.516
Normal
XP_002520978.1
No significant match
-
CFeSSR38
2
0.625
0.458
0.337
Normal
NP_190912.1
No significant match
-
CFeSSR41
5
0.75
0.817
0.746
Normal
ABY48133.1
hydroxyproline-rich glycoprotein [Medicago truncatula]
4.00E-16
CFeSSR46
3
1
0.592
0.457
Normal
XP_002299081.1
tir-nbs resistance protein [Populus trichocarpa]
6.00E-18
CFeSSR48
2
0.429
0.363
0.28
Invariant
ABF99421.1
DHHC zinc finger domain containing protein, expressed [Oryza sativa Japonica Group]
2.00E-06
CFeSSR51
2
0
0.5
0.359
Normal
-
No significant match
-
CFeSSR53
3
0.625
0.608
0.557
Normal
ACN12965.1
R2R3-MYB transcription factor MYB22, partial [Pinus taeda]
2.00E-22
CFeSSR54
2
0.25
0.233
0.195
Invariant
-
No significant match
-
CFeSSR58
2
0.143
0.143
0.124
Invariant
XP_002525131.1
bromodomain-containing protein, putative [Ricinus communis]
3.00E-08
CFeSSR61
2
0.25
0.4
0.305
Normal
XP_003638563.1
LCR/BET1-like protein, partial [Medicago truncatula]
7.00E-53
CFeSSR63
2
0.143
0.143
0.124
Normal
NP_180257.3
DNAJ heat shock N-terminal domain-containing protein [Arabidopsis thaliana]
5.00E-24
CFeSSR72
5
1
0.808
0.74
Normal
NP_001060813.1
Os08g0109800 [Oryza sativa Japonica Group]
5.00E-06
CFeSSR79
2
0.125
0.125
0.11
Invariant
XP_003617683.1
Mitochondrial import inner membrane translocase subunit TIM22 [Medicago truncatula]
1.00E-13
CFeSSR82
3
0.125
0.342
0.3
Invariant
NP_563772.1
phospholipase A1-Igamma1 [Arabidopsis thaliana]
2.00E-76
Heterozygositya
SSR locus
Na
χ 2 test of
BLASTX top hit
(NCBI accession)
Ho
He
PIC
Segregationb
BLASTX
Putative function at E≤10− 5 [organism]
E-value
CFeSSR87
3
0.875
0.658
0.561
Normal
-
No significant match
-
CFeSSR93
3
0.75
0.608
0.505
Normal
ADM52965.1
nudix hydrolase [Triticum aestivum]
6.00E-16
CFeSSR97
4
0.875
0.758
0.69
Normal
NP_001045148.1
Os01g0909200 [Oryza sativa Japonica Group]
2.00E-55
CFeSSR98
2
0.375
0.525
0.371
Invariant
ADV71374.1
glycosyltransferase GT21C20 [Pueraria montana var. lobata]
3.00E-22
CFeSSR99
3
0.875
0.608
0.499
Normal
NP_001242444.1
uncharacterized protein LOC100820619 [Glycine max]
3.00E-118
CFeSSR100
3
0.5
0.542
0.431
Normal
NP_001146214.1
uncharacterized protein LOC100279784 [Zea mays]
3.00E-78
CFeSSR108
3
0.25
0.242
0.215
Invariant
-
No significant match
-
CFeSSR123
2
0.375
0.525
0.371
Normal
ACM17914.1
dehydration-responsive element binding protein [Aloe vera var. chinensis]
3.00E-10
CFeSSR127
2
0.375
0.325
0.258
Invariant
-
No significant match
-
CFeSSR141
2
0.375
0.525
0.371
Normal
NP_001047717.2
Os02g0674700 [Oryza sativa Japonica Group]
1.00E-59
CFeSSR143
3
0.75
0.542
0.428
Normal
XP_002526440.1
rRNA-processing protein EBP2, putative [Ricinus communis]
1.00E-41
CFeSSR148
2
0.833
0.53
0.368
Invariant
-
No significant match
-
CFeSSR151
3
0.25
0.567
0.47
Normal
EFA82948.1
No significant match
-
CFeSSR168
4
0.875
0.725
0.646
Normal
XP_002886139.1
oxidoreductase [Arabidopsis lyrata subsp. lyrata]
8.00E-142
CFeSSR182
3
0.5
0.642
0.522
Normal
NP_001078129.1
aspartate/glutamate/uridylate kinase-like protein [Arabidopsis thaliana]
4.00E-14
CFeSSR190
4
0.625
0.642
0.559
Normal
NP_191739.1
uncharacterized protein [Arabidopsis thaliana]
6.00E-131
CFeSSR199
3
1
0.658
0.551
Normal
NP_199707.2
HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase [Arabidopsis thaliana]
0
CFeSSR201
3
0.625
0.692
0.612
Normal
AEW07600.1
anonymous locus 0_6465_01 genomic sequence[Pinus taeda]
5.00E-21
CFeSSR203
2
0.375
0.325
0.258
Normal
NP_001052667.2
Os04g0397500 [Oryza sativa Japonica Group]
5.00E-70
CFeSSR206
2
0.25
0.233
0.195
Normal
NP_001048375.1
Os02g0794300 [Oryza sativa (japonica cultivar-group)]
0.0000007
CFeSSR207
3
0.75
0.608
0.505
Normal
XP_001772279.1
single bromodomain-containing protein [Physcomitrella patens subsp. patens]
1.00E-35
CFeSSR212
2
0.25
0.233
0.195
Normal
-
No significant match
-
CFeSSR215
2
0
0.5
0.359
Invariant
ABD83289.1
GlimmerM protein 152 [Beta vulgaris]
5.00E-09
CFeSSR220
3
0.625
0.708
0.618
Normal
-
No significant match
-
Heterozygositya
SSR locus
Na
χ 2 test of
BLASTX top hit
(NCBI accession)
Ho
He
PIC
Segregationb
BLASTX
Putative function at E≤10− 5 [organism]
E-value
CFeSSR229
3
0.375
0.542
0.428
Invariant
NP_974447.1
alpha-galactosidase [Arabidopsis thaliana]
8.00E-16
CFeSSR234
2
0.25
0.233
0.195
Normal
NP_001154682.1
ubiquitin thiolesterase [Arabidopsis thaliana]
3.00E-22
CFeSSR249
3
0.143
0.275
0.24
Invariant
AEW08545.1
anonymous locus CL376Contig1_05 genomic sequence [Pinus taeda]
5.00E-29
CFeSSR250
3
1
0.708
0.609
Normal
-
No significant match
-
CFeSSR253
4
0.5
0.525
0.488
Normal
-
No significant match
-
CFeSSR254
3
0.375
0.342
0.295
Invariant
XP_002527890.1
r2r3-myb transcription factor, putative [Ricinus communis]
5.00E-10
CFeSSR260
3
0.75
0.7
0.599
Normal
NP_565651.1
sec23/sec24 transport family protein [Arabidopsis thaliana]
1.00E-26
CFeSSR265
3
0.875
0.658
0.551
Normal
-
No significant match
-
CFeSSR267
3
0.286
0.473
0.391
Normal
AAP31312.2
ABI3-interacting protein 2 [Callitropsis nootkatensis]
2.00E-07
CFeSSR271
3
0.5
0.708
0.618
**
NP_001055368.1
Os05g0373500 [Oryza sativa (japonica cultivar-group)]
1.00E-08
CFeSSR272
3
0.875
0.658
0.593
Normal
CBN75958.1
similar to deltex 3-like [Ectocarpus siliculosus]
9.00E-34
CFeSSR276
3
0.75
0.575
0.509
Normal
-
No significant match
-
CFeSSR277
2
0.375
0.325
0.258
Normal
-
No significant match
-
CFeSSR278
2
0.625
0.458
0.337
Normal
-
No significant match
-
CFeSSR281
2
0.75
0.5
0.359
Invariant
XP_002531256.1
UDP-N-acetylglucosamine transporter, putative [Ricinus communis]
5.00E-65
CFeSSR284
2
0.25
0.233
0.195
Normal
ACG29023.1
beta-amylase [Zea mays]
0
CFeSSR291
4
0.857
0.626
0.524
Normal
-
No significant match
-
CFeSSR297
2
0.125
0.458
0.337
Invariant
-
No significant match
-
CFeSSR301
2
0.375
0.325
0.258
Invariant
-
No significant match
-
CFeSSR302
2
0.25
0.233
0.195
Normal
gb|AAR28378.1|
EIX receptor 2 [Solanum lycopersicum]
1.00E-48
CFeSSR310
3
0.5
0.625
0.559
Normal
NP_568528.2
O-fucosyltransferase family protein [Arabidopsis thaliana]
0
CFeSSR312
4
0.75
0.758
0.69
**
NP_001064833.1
Os10g0473400 [Oryza sativa Japonica Group]
0
CFeSSR314
2
0.25
0.233
0.195
Invariant
XP_002274510.1
PREDICTED: similar to AT5g65000/MXK3_23 [Vitis vinifera]
1.00E-69
CFeSSR319
3
0.75
0.542
0.431
Normal
BAA19112.1
PEThy;ZPT3-1 [Petuniax hybrida]
6.00E-23
Heterozygositya
SSR locus
Na
χ 2 test of
BLASTX top hit
(NCBI accession)
Ho
He
PIC
Segregationb
BLASTX
Putative function at E≤10− 5 [organism]
E-value
CFeSSR321
2
0.375
0.325
0.258
Normal
XP_002885042.1
ubiquitin-specific protease 25 [Arabidopsis lyrata subsp. lyrata]
2.00E-121
CFeSSR324
4
0.75
0.617
0.572
Normal
-
No significant match
-
CFeSSR326
2
0.125
0.325
0.258
Invariant
XP_001772279.1
single bromodomain-containing protein [Physcomitrella patens subsp. patens]
7.00E-21
CFeSSR328
3
0.625
0.508
0.429
Invariant
NP_200863.2
geminivirus rep interacting kinase 2 [Arabidopsis thaliana]
2.00E-104
CFeSSR331
3
0.875
0.625
0.542
Normal
Q9CAE3.1
RecName: Full=Lysine-specific histone demethylase 1 homolog 3
0
CFeSSR337
2
0.25
0.4
0.305
Normal
XP_002871469.1
pentatricopeptide repeat-containing protein [Arabidopsis lyrata subsp. lyrata]
2.00E-120
CFeSSR340
2
0
0.5
0.359
Normal
XP_002298618.1
f-box family protein [Populus trichocarpa]
3.00E-29
CFeSSR342
3
0.625
0.667
0.563
Normal
-
No significant match
-
CFeSSR343
5
1
0.775
0.715
Normal
CAD39696.3
OSJNBb0115I21.12 [Oryza sativa Japonica Group]
9.00E-22
CFeSSR351
2
0.125
0.125
0.11
Invariant
AEW08311.1
anonymous locus 2_6183_01 genomic sequence [Pinus taeda]
1.00E-23
CFeSSR352
2
0.25
0.233
0.195
Normal
XP_001755522.1
PINB AtPIN5/6-like auxin efflux carrier protein [Physcomitrella patens subsp. patens]
3.00E-28
CFeSSR353
3
0.375
0.342
0.3
Normal
AEW26787.1
putative ribosomal S6 kinase [Physcomitrella patens]
3.00E-23
CFeSSR362
2
0.375
0.325
0.258
Invariant
-
No significant match
-
CFeSSR369
2
0.375
0.325
0.258
Normal
XP_002870275.1
protein translocase [Arabidopsis lyrata subsp. lyrata]
5.00E-18
CFeSSR374
2
0.375
0.458
0.337
Normal
NP_190684.2
kinesin motor family protein [Arabidopsis thaliana]
1.00E-53
CFeSSR387
3
0.75
0.542
0.504
Normal
XP_002888835.1
kinase family protein [Arabidopsis lyrata subsp. lyrata]
2.00E-71
CFeSSR389
2
0.375
0.325
0.258
**
NP_001051061.1
Os03g0712100 [Oryza sativa Japonica Group]
1.00E-88
CFeSSR391
2
0.25
0.233
0.195
Invariant
XP_002513525.1
nucleic acid binding protein, putative [Ricinus communis]
4.00E-85
CFeSSR400
2
0.25
0.233
0.195
Invariant
ADB85366.1
putative uvrB/uvrC motif family protein [Phyllostachys edulis]
1.00E-69
CFeSSR404
3
0.5
0.425
0.354
Normal
NP_199943.1
splicing factor 1 [Arabidopsis thaliana]
3.00E-124
CFeSSR405
3
0.875
0.625
0.524
**
XP_002887115.1
zinc finger family protein [Arabidopsis lyrata subsp. lyrata]
6.00E-106
CFeSSR418
4
0.5
0.442
0.392
Normal
NP_196224.1
putative methyltransferase PMT12 [Arabidopsis thaliana]
0
CFeSSR424
2
0.125
0.125
0.11
Invariant
AFG53516.1
nonymous locus UMN_5015_01 genomic sequence [Pinus taeda]
1.00E-51
CFeSSR428
2
0.125
0.125
0.11
Invariant
NP_193167.3
transducin/WD40 domain-containing protein-like protein [Arabidopsis thaliana]
0
Heterozygositya
SSR locus
Na
χ 2 test of
BLASTX top hit
(NCBI accession)
Ho
He
PIC
Segregationb
Putative function at E≤10− 5 [organism]
BLASTX
E-value
CFeSSR431
2
0.167
0.409
0.305
Invariant
XP_002528785.1
RNA binding protein, putative [Ricinus communis]
3.00E-88
CFeSSR435
2
0.375
0.325
0.258
Normal
NP_001052690.1
Os04g0401800 [Oryza sativa Japonica Group]
1.00E-89
Mean
2.64
0.479
0.462
0.383
Note：a: Heterozygosity base on a set of 8 unique individuals. Na = number of alleles detected; Ho = observed heterozygosity; He = expected heterozygosity; PIC = polymorphic
information content.
b
: Segregation analysis statistics base on a set of 14 progeny from a “D110” ד6421” cross and their parents. **: Derived progeny displayed segregation distortion at the p < 0.05
level.