Figure S2 Sf101 phage Rhizobium sp. CF080 Thiothrix nivea Cupriavidus metallidurans CH34 Burkholderia sp. CCGE1003 Azospirillum sp. B510 Pseudomonas aeruginosa Nitrospira defluvii Competibacter phosphatis Dechloromonas aromatic RCB Pseudomonas sp. GM55 Pseudomonas fluorescens F113 Rhodobacter capsulatus Pseudomonas sp. S9 Flavobacterium columnareATCC49512 Klebsiella pneumonia MGH78578 Serratia fonticola Acinetobacter pittii Enhydrobacter aerosaccus Burkholderia ambifaria 14 24 22 15 14 14 11 51 11 11 11 11 11 8 11 4 14 18 14 28 Sf101 phage Rhizobium sp. CF080 Thiothrix nivea Cupriavidus metallidurans CH34 Burkholderia sp. CCGE1003 Azospirillum sp. B510 Pseudomonas aeruginosa Nitrospira defluvii Competibacter phosphatis Dechloromonas aromatic RCB Pseudomonas sp. GM55 Pseudomonas fluorescens F113 Rhodobacter capsulatus Pseudomonas sp. S9 Flavobacterium columnare ATCC49512 Klebsiella pneumoniae MGH78578 Serratia fonticola Acinetobacter pittii Enhydrobacter aerosaccus Burkholderia ambifaria SVILMSLLAVGLFDKISPINLVEHGRNNQIDGMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKIISGDQ-----D ACMMFASGLAVKSALISPVVTASSNRYGALDGLRGLLAYGVMFHHSMAAYSYFSSGVWYGLDNAVVFQLGKTTVALFFMITGFLFTEKCITGKVN----VFMFIVASYFIRQDTFAKSLTVPSGRYGSLDGLRGVLATGVFIHHAFAMYVYFSGGEWQWSTSPVFNQLGQTTVSLFFMITGFLFTLKALAPTID----LCLALVWPKR-LWRFLDDPPSGQGQRYVTVDGLRGFLALAVVLHHCVISYGFHQTGEWKLPPSSFYSIIGQVGVSIFFMITAFLFWGRLLDQGKR---LD AILCCLFALP-IFAFVDD-AAGVKERMQTIDGLRGFLAASVMVYHGLINYNYVTVGKWMAADALFYRPLGGVAVMFFFMITAFLFWTRLLRRDGR---PD VAVCVSWAILRLIAPRLRPQPGSDARYAGIDGLRGLLAFAVFIHHGVITWQYLHTGVWALPPSRLHTHLGQSGVALFFMVTAFLFWDKLLKAGPG---MD VCVALALLTCGIIRYLKP-IPLPLSRFSTIDGLRGYLAFFVFLHHAAIWYYFLRSGAWQVPPSNLYTHFGQTSVSLFFMITGFLFTHKLLQSKNR--PID AALLLALGTATLLPEITPRQATSPVRYTSLDGLRGYLAFAVFLSHSSIWYFYLRSGTWDVPPSNFYAHLGQSSVILFFMITAFLFWSKLLDGRVQ--PVD LILYVAVVVAYLMSRYSE-IPPDQGRFVALDGLRGYLALFVFLYHSALWYFYLRTGQWEVPPSNLYTHFGQSSVLLFFMITGFLFFSKLLDGRTR--GID FAILMALFSCFVLIRKFG-PPEEQGRYLAIDGLRGYLAFCVFLHHSIIWFFYLKTNQWAVPPSNLYTHFGQTGVAFFFMITGFLFFSKILEGRGK--EID VAVAFALLSTLAISSQLP-PPSSHGRFLTIDGLRGYLAFFVFLHHSCIWYYYLQTGAWVAPPLRLFVHFGQVGVSLFFMITGFLFFSKIMDDRGR--GVD IAILVALLSTNLFLGKLV-VPSSHGRFVTLDGLRGYLAFFVFLHHSYIWYYYIHSDVWVLPSSRLFVHFGQVGVALFFMITGFLFFNKLLEGRAR--GID IVFSIALITVFLLAGLLR-IVPQDDRVSTIDGLRGYLACGVFLHHSAIWYYYLHTGKWVAPPSHLYAHLGQTSVALFFMITGFLFYSKILSSR----PLD IATGIALTAALCVQKIVP-VHDSSHRHTAIDGMRGYLAFFVFLHHSSIWYFYLHEGRWALPPSNLFSHLGQSSVALFFMITGFLFYSKILSSRDK--KIS IIFFIAFTTAYIINLKFK-IINNNTRYETIDGIRGFLAIGVFIHHASIWFQYLQIKSWVAPKSNLYNQLGQTSVSLFFMITSFLFITKLLNSENQ--KIN KYISNKLGIELINS----------GRFTAIDGLRAYLSLFVFFHHFFIFYKWKESGKWEKPEILFIDDLGQVSVAIFFIITGFLFIGKITSGR----HFD AMIFCVLFMKIINSRLIKIELPIGNRAGCIDGARGYLALMVAVHHYYIFYFWIKTGKWLPPEVQYINSLGKVAVSAFFIITGYLFLGKILRDNRLHIKTQ SVTIFSLIFTKIN----ILPETNSGRTSTIDGLRGILALSVMTHHFYITYIWKTVGEWKKPENILIDNFGGVAVSLFFLITGYLFISKIRKDE-----VS SLTLVTWLLNQLPQRM-IVGFDASHRHSPLDGIRGILALSVFTHHFFKNYFFQTTGRWQSPNIDFFTNLGSVPVSLFFLITGYLFFGKLKQPW-----MD VAIIAWLVARHVPFYRSAVDKSLSMRHPNLDGFRGILATSVVFHHFACNQGLLSTGVWGAP-SDFLRNLGTIPVALFFMVTGFLFWERACRGT-----LD WTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPFTAL-GMPNINDVKDSFTINAAVTWTLVYEWFFYFS-LPVISALIKRKV WRSLYISRLFRLAPLYILVMLVMFTVIFALSGFQVLVPISKLGSQLGKWLLFGAV-GRPNINGFEKSWTLIAGVNWSLRFEWQFYLFGLPVLFLMSRFMN WKRLYASRVLRLVPLYALIVSSIFGLVFYLSNWALNEPVWEIIKEYWVWMTFVCF-GRPDINAYPMSWTLIANVNWSLKYEIMFYLFAVPAIHVFSRFVQ WFALYVNRFLRIAPLYWVVVALMLLVVAIKTGFTLAVPPSELVKQVFQWALPAIVRGMPPVNGYQQTSTITAGVTWTLYYEWMFYFS-LPFLAIAAIKRS WAALYIGRFFRIAPLYIFAVLAMTFIVFWRSGFELREQLGIFLPNMGRWLAFGLNDDMQPINAFPWTIFILMGVTWSIKYEWWFYFS-LVVSAVFVRTGM WTGFLSSRFHRLYPVYAVAVLLTMILALAATGFEFRTGPLDLLRRLIGWATFKAP----PINGLENAGQIVAYATWSLPYELLFYAA-LPALALLVSPVR WLQLYISRFMRIYPAYIFAIAIMFTIAFFMTGYTLHESVLSLLKKTIQWGAFRTP----DINGLVETRRIMAGVTWTLPYEWLFYLC-LPALSLLIGRRA WSRLYLARVFRLVPLYLLVVIGVVSIALYRAGFRLQESMTSFALHLASWLTFTIP-GIAPVNGFAET-IPLAGAVWSLPYEWLFYAC-LPIGSWCIRAAT WLKLFIGRLLRLVPLYLFMIVSLCIIVMVVSGWVLHEPLLKIAEHLVCWLGFTIL-GAPDLNGVKDTLLIVSGVIWTLPYEWFFYLI-LPVLAMTVRVVP WLRLYVSRFLRLMPLYAVAMGALFLIVFSLSGATLHEPISVLASEVLRWMAFTVR-GNPDINGIERTSLIMAGVTWSLPYEWCFYML-LPVFALVNRVRV WLRLYVSRFLRLTPLYFFSMILLFSIVGWVTKENQTDSFSELIGACLKWLGFTIYFGVPDINGFPGTYVVNAGVTWTLPYEWTFYMA-LPLIALAIGVWP WFRLYVSRFLRLTPLYLFSMVLLFLIVWVLTKNEPAQPTGKLIVDGLKWIGFRVS-GAPDLNGLLGTRYIHAGVTWTLPYEWFFYLF-LPFVALAIGSRP WTRIFVSRLLRLTPLYLVVISLMLIVVGVQTQWELREPLYNLINNIIRWFLFLQA----NVNQMEETRTITAGVTWTLPYEWSFYFL-LPALALFTGR-WTYIYTSRFMRLVPLYLLAISILLLFVFIKTNWKIETGLSSILIDATKWVLFTIP-GAPDINNVKHTSLIMAGVTWSLPYEWLFYFS-LPLIALTTGLSR WPIIFISRFFRLVPMYLVSIFLLISIVFIISDWQLNVTPFKLLKEVLQWGTFTIL-SSPTINDLSFTHIINAGVVWSLPYEWLFYFS-LPIISILIFKKK FFSVYISRFFRIMPLYLLVVMITLFYSFLLTGLSIQIPHSELLKDIVRWLLFVGD----SVNGYTDAKRITAGVTWTLKYEWLFYFS-LPLLFLILKNKV WSPLYLSRFLRITPLYLLSVLVTIILTFVLSEGVLYVSFFELFKEVSRWLLYVGY----SINGDADARRITAGVTWTLKYEWVFYLT-LPILAILLKERT WKQIYISRIKRIIPLYLFVFLFILAITLLN-VQITASNYIEFLKWVSDWILFKGG----SFQ-NFESGLVIAGVHWTLIYEWKFYFA-LPLIFVIWQ-QK WQYLYRSRLQRIVPLYLFLGICIVAIYFT--QQHPPLPLSEWQQWTLRWLSFNNQ----SLK-DFYAWPLVAGAAWTLLYEWGFYFS-LPLLFVLVHPKQ VRSFFVGRVRRVAPLYLFYATVIVACTLYWFPRNVVDAPIRLLADLLKAVSLGWF-GAFPVNGAEHTGYLSG-VWWTLAYEWRFYVA-IPFLAWFVATRA 108 118 116 110 108 110 107 148 107 107 107 107 105 104 107 89 113 108 107 121 206 217 215 209 207 205 202 245 205 205 206 205 198 202 205 184 208 200 199 218 Sf101 phage Rhizobium sp. CF080 Thiothrix nivea Cupriavidus metallidurans CH34 Burkholderia sp. CCGE1003 Azospirillum sp. B510 Pseudomonas aeruginosa Nitrospira defluvii Competibacter phosphatis Dechloromonas aromatic RCB Pseudomonas sp. GM55 Pseudomonas fluorescens F113 Rhodobacter capsulatus Pseudomonas sp. S9 Flavobacterium columnare ATCC49512 Klebsiella pneumonia MGH78578 Serratia fonticola Acinetobacter pittii Enhydrobacter aerosaccus Burkholderia ambifaria SIYMVMISAISLFVFILFFS--------KIHIVSFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMIFEMTYFKTTYA-PLPLILCGITFIIIASGCDL RKALLTGVLTGLGTLALYGWIRGDLGLELLCTAHFLCGIAASLIYNNSVGKRLITSRAFHAAALFCLPVLATSPQGTVV--PDLLATFAIFLSVLGGGSL LRAALVLCAAMLGAILLFRWQRGGEGGISLYTAQFLGGVMVAYAFKIDRLKAVIEGNIIRWLAAGALVSLFFMQYAYSA--VAVIGNIILFAAVVGGASL PLAFVPACIWIVFVMPETLSS----AFARNLVAMFVMGMVAASLVRRSPGFRGD-SVLKSAIAVALLAFPLLTRSTAYE-SVSILSLGAFFILVSSGASL HMVFALVGLAASFAIILTTQS----DLWC-LPAAFFCGMVTASLLHENVRPRML-ENTMSLIALAALALLFSFAKTHAG-VVQIALTGIAFYMMACGADL RLRPALVSLVLTLALLLYFR-----NFSLNVLQCFLGGIAAAYAIRQPRFVRFARSDRGLVLALAALLATVAGFPGAYA-PGPVLGLGLFFAIVAAGQDF PMT-ALATTIIIASLILSFW-----RPSPILLCMFLAGGIAALTARSEWLQSLSNGRLGSLLCVCLIGSAVIIFPTAYT-LGPAILLSLAFILITAGCSI PTSWLLFSAAAVVTLTTVMP-----DVRSPVLYAFGGGIAAASLARVDAIRTRLTHRVRGLVVVACLGITMWLFETAYT-VPALLLLTGAFTIIACGNSI PPLYIVLSVGSIVTFIIYNPQTHPVHPQLHPLFSFFGGIAASLLVRSDSFRWFCRKDYCSFLVIGLIIAVVVLFQTAYA-IMPLIFISLIFSLIAGGNSL GRVYIIFSV-LVLIFIFKN------HTYMYHWLSFVGGIAAAYLVRIDYLRYLLRKKVFSMAAILLISATVAFFPSTYD-WAPIAMLSAVFVMIAAGNGV PVKYLLLSVLSMYAFGYLY------LAGSFYWL-FLGGMVAAILVRFESFRAVAVSRWATLIVLVLAVYVVTMYPSVYDYVQAKFLLVIVFCFIAGGNSI PVKYLCLAAIALYVFDVYG------YSWSFGWL-FLGGMVAAVLVRYDRFTIFSVSKWATCLIVGSLAWSVTYYPTIYESSVPRLLLIAVFCLISGGNTL PVPVIILPIVALSAFYMYFAG-----LRPSRFVFFLGGIGAAFLARRSWFCQLAAHKASSLVILASMTCLITLFPSAYG-KIQLVLIFIAFSLVAAGNSL N-LTAAAVSLAVVLLIYTVFD-----PATIHIKTFIDGMLCAALHHMKIMTGLSSSRLYSAFIIILLYVLVTTYQSSHG-NIQILILSLVFFGISGGNNL TSFFYTVISLFFILCFFKIYG-----SSIPHLLSFLGGIIPPFIIKYNTKKINFNSNFYS-IIILLCLGLILLFHTSDN-YICKLLIIIVFNLIALGNEM ATTLIIIVCMLLLINNFKFHSI-IDSRYFIFFMIGGISNYICKLLENNIKLIEILRNRLISIILMVLLIYVFFSGVGIFNIFSIISLLLFFIAIVCGNNL LAIISLIVGVG--ISYFNINGFGFESHYLIYFIFGGGIAFVQSL--NNKKLSGMMSTSIWSVISILSLVFVIFITPHYSVFLSDILILFFLMPIIFGNSL IPKWIS-SILVIAF--MVYIFKHKSHHLYALFFL----AIPAVLYKDRFKQFMQTKPAITHIVLGILSIIVLFFTEAYS-WFQMLSLAVIFSFIVSGYSVCQWQNIAMLMISLPVLYYVFTHTIIKLYWLFLL----AFFAVWLEDFFKNLLAKFPVVFAIALCLLTLFILFETSAYS-YWQMLLCGLLFCFIANGFTY WRKLLALAIVTFCAALFGPPG--------GMALLFAFGALAFEASRHRAIRSLLGTKSAATVALLILAVAPYP-AERYS-IEGALPLLPVFLCVACGNTF 297 315 313 303 300 299 295 339 304 297 299 298 292 295 298 283 304 291 294 308 Sf101 phage Rhizobium sp. CF080 Thiothrix nivea Cupriavidus metallidurans CH34 Burkholderia sp. CCGE1003 Azospirillum sp. B510 Pseudomonas aeruginosa Nitrospira defluvii Competibacter phosphatis Dechloromonas aromatic RCB Pseudomonas sp. GM55 Pseudomonas fluorescens F113 Rhodobacter capsulatus Pseudomonas sp. S9 Flavobacterium columnare ATCC49512 Klebsiella pneumoniae MGH78578 Serrati afonticola Acinetobacter pittii Enhydrobacter aerosaccus Burkholderia ambifaria YGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPNNYTENTF-----IILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ-WGLLKTRAAIWLGDISYGVYLIHGLALWLSYYNLSRAGLLDAID-GPIFLLLLPAIGLVVLAISSVSYIRVELPMIKLG--RKLGSPKTSSRVELA---FGLLKHKSAVWLGDISYGIYLMHGLVLWLVLNMLAKAGILSQLG-LVGYWLVTFATAASIVLLASCSYALLEKPVMGLLPARKQDRVRGYKQPGLRTW-FGLLASRSAVRLGSVSYGIYLLQGIVITVLHSP-RVLGAFACKG-PEQFWLTTIAVGLVLVCVAAASYHFVERPCIRLGKKIGKAPESARQTHVEMGDGT FGLLRLKSAQRLGHVSYGVYLLQGIPITLLFWH-APFRAWAVVS-PMHYWLALLVCAALLCVVAALTFALIERPFIQLGKSIHSPAS-------WLKAGA RGTLTRQPVLWLGEISYGVYLLHGIVLWTLITANGPLRALIGAD-ESLYLLALTVAGVLVVALASLVHLTVERPAIRFGKRSRDPRQ------------A FGLLNLSVSRFFGEITYSLYLLHGIVLYVIFEIVLGNQAAKEFD-TRQHWMIIYAATPITVVLSYLSFRYIERPSMSASKGLGARIKS----LLRNTYRP YGILEWPTSVLLSEMGYSLYLLHSLLLFAVYRLVLG-EWASTLS-ATEHWSVVLALVPILIVLCFATFRLIEQPAMAAVPRCHAWLVA----HLNE--RP FGILRSSISRVLGELSCSIYLLHGMALFILFKFVVGPNHAQLLS-AQQHWMLVIAISPVILFISYWTFRLIEQPAMRATNTVTVWVSSRLTWRFRTYVVS FGLLTNAVSRALGELAYSIYLLHGIVLFLLFRYVIGFEKSKLLQ-PIDYWSAIIAVSPILIFISYLSYRKIERPALLSTDGLTSWLRN----KINSYSEA FGVLNSKVSRVMGEMAYSIYLLHGLVLFVAFRLVIGASLGKALS-PSQYWGVIVTLTPVLILICGLTFRFIERPAMKMTGPLTDWIRA----KKTNVIAG FGLLKLKVSLVMGEMAYSMYLLHGVLLFVIFRFIFGGARASELT-PLQYWGVIVLATPILMMICGLTFRFVERPAMRSVDTLTHWIRA----KKKGRFER FGALTNRVSRALGEITYSIYLLHGLILYSLMKLVLFPDTNAALPSPFAFWCIVLCVTPVLITMSMLTFKFIEQPAMQHADTLATRLKASWTAFRSRDARA FGVLSWKTSRVLGEMAYSIYLLHGLILFTTFNFIINSETSIELT-PYQYWILIFAIAPAVVIISAITFVMIEKPSMDKTQKIANQICN---LSSKK---FGVLKNTTLKFLGEISYSTYLIHGIIIFITLYFGFSLEVVEKMS-PSTFCSIIFLITPIIILTSFLSYRNIEKPFMDYSKKINYDKIN---YSITEFYKR FGALTLKGARLLGEISYSIYLIHGCVIFSIF-ILMNKFSGLS-LSEYLILMPFVTIA--VVFISSLTYRFIEAPCINIGKKIQNIYRFNGKKAL-----FGLLKLKSSIRLGEISYSIYLMHGIVFFVIF-IFLFDVNKMNGWVEFFIYMPFSLML--VVFFSVYTYQYIEKPFVDLSHIIR-KKRINTNEII-----FGILNHKGLKVLGEISYSIYLIHGLVLYTIFTVINIVDLKTISLEKYYSFFLPTALL--VTIVSLFTYKFIECPFLRRPLKKL----------------FGVLYQQGLKVLGELSYSLYLTHGLVMYIWFNLLHLHDFS-QSMTAYVWTYPLVLVF--VVMFAVIGYNYIEKPLMHRPNSRVLSP-------------YGVLQTRPLALLGTASFSIYMLHMAIVYALVRAFNKFVFPINSVDDSGIWALSFACALVAVFCALLTYRYIEHPFIAKRNDARPARRTIEPAREQSTAAL 390 408 410 401 391 386 390 431 403 392 394 393 392 387 394 373 394 372 377 408