1
1.5 Kb
2
3
4
5
6
7
8 9
750 bp
a
b
b
Fig. S1 Agarose gel electrophoresis of (a) Bacterial specific 16 S rDNA and (b) Fungal specific
ITS amplicons from metagenomic DNA isolated from various soil samples. Lane 1- Amplified
product from purified forest soil metagenomic DNA, Lane 2 – No amplification in control forest
soil metagenomic DNA, Lane 3- Amplified product from purified biocompost metagenomic
DNA, Lane 4 – No amplification in control biocompost metagenomic DNA, Lane 5 – 1Kb
(NEB) ladder, Lane 6- Amplified product from purified termite nest metagenomic DNA, Lane
7– No amplification in control termite nest metagenomic DNA, Lane 8- Amplified product from
purified mangrove forest metagenomic DNA, Lane 9 – No amplification in control mangrove
forest metagenomic DNA (b) Fungal specific ITS PCR amplicons from DNA isolated from
various soil sample using the optimized protocol. Lane 1- Amplified product from purified forest
soil metagenomic DNA, Lane 2 – No amplification in control forest soil metagenomic DNA,
Lane 3- Amplified product from purified biocompost metagenomic DNA, Lane 4 – No
amplification in control biocompost metagenomic DNA, Lane 5 - Amplified product from
purified termite nest metagenomic DNA, Lane 6 – No amplification in control termite nest
metagenomic DNA, Lane 7- Amplified product from purified mangrove forest metagenomic
DNA, Lane 8 – No amplification in control mangrove forest metagenomic DNA, Lane 9 –
Fermentas 1 Kb ladder