Strain/Plasmid
Strains
E. coli
DH5α
BW25113
ET12567
EPI300TM
BL21(DE3)
S. lividans
Strain 1326
S. xiamenensis
Strain 318
ximA
ximB
ximC
ximD
ximE
Plasmids
pMD18-T
pET28a+
pET Duet-1
pCC2FOSTM
pJTU1278
pSET152
p9A11
pLMO09403
Table S1. Strains and plasmids used and generated in this study
Purpose
Source
Host strain for cloning
Host strain for PCR targeting
Donor strain for conjugation
Host strain for genomic library
Host strain for protein expression
Invitrogen
Smanski et al. (2011)
Li et al. (2008)
Epicentre
Invitrogen
Host strain for heterologous expression
Xiamenmycin producing strain
ximA inactivation mutant of strain 318
ximB inactivation mutant of strain 318
ximC inactivation mutant of strain 318
ximD inactivation mutant of strain 318
ximE inactivation mutant of strain 318
Xu et al. (2009)
This study
This study
This study
This study
This study
Ampr. vector for cloning
Kanr. vector for protein expression
Ampr. vector for protein coexpression
Fosmid vector
Ampr. Tsrr. vector for PCR Targeting and
conjugation
Aprr. vector for heterologous expression
Chlr. vector for amplifying genes
Ampr. Tsrr. 7.5 kb HindIII-XbaI fragment cloned
TAKARA
Novagen
Novagen
Epicentre
in pJTU1278, containing xim gene cluster
He et al. (2010)
Flett et al. (1997)
This study
This study
Ampr. Tsrr. Aprr.subclone for ximA inactivation
This study
Ampr. Tsrr. Aprr.subclone for ximB inactivation
This study
Ampr. Tsrr. Aprr. subclone for ximC inactivation This study
Ampr. Tsrr. Aprr.subclone for ximD inactivation
This study
Ampr. Tsrr. Aprr.subclone for ximE inactivation
This study
Aprr. 7.8 kb EcoRI-XbaI fragment cloned in
pLMO09404
pSET152, containing xim gene cluster for This study
heterologous expression
Kanr. protein expression clone for ximA
pLMO09405
This study
expression
r
Kan . protein expression clone for ximB
pLMO09406
This study
expression
r
Kan . protein expression clone for ximC
pLMO09407
This study
expression
r
Amp . protein expression clone for ximD and
pLMO09408
This study
ximE coexpression
r
Amp . protein expression clone for ximD
pLMO09409
This study
expression
Kanr. protein expression clone for ximE
pLMO09410
This study
expression
Ampr, ampicillin resistance; Tsrr, thiostrepton resistance; Aprr, apramycin resistance.
pLMO09403-1
pLMO09403-2
pLMO09403-3
pLMO09403-4
pLMO09403-5
Reference：
Li, L., Xu, Z., Xu, X., Wu, J., Zhang, Y., He, X., Zabriskie, T. M., Deng, Z. (2008). The mildiomycin biosynthesis:
initial steps for sequential generation of 5-hydroxymethylcytidine 5'-monophosphate and 5hydroxymethylcytosine in Streptoverticillium rimofaciens ZJU5119. Chembiochem 9, 1286.
Smanski, M. J., Yu, Z., Casper, J., Lin, S., Peterson, R. M., Chen, Y., Wendt-Pienkowski, E., Rajski, S. R., Shen,
B. (2011). Dedicated ent-kaurene and ent-atiserene synthases for platensimycin and platencin biosynthesis.
Proceedings of the National Academy of Sciences 108, 13498.
He, Y., Wang, Z., Bai, L., Liang, J., Zhou, X., and Deng, Z. (2010). Two pHZ1358-derivative vectors for efficient
gene knockout in streptomyces. Journal of microbiology and biotechnology 20, 678-682.
Flett, F., Mersinias, V., Smith, C. P. (1997). High efficiency intergeneric conjugal transfer of plasmid DNA from
Escherichia coli to methyl DNA-restricting streptomycetes. FEMS Microbiol Lett 155, 223-229.
Xu, J., Wang, Y., Xie, S.J., Xiao, J., and Ruan, J.S. (2009). Streptomyces xiamenensis sp. nov., isolated from
mangrove sediment. Int J Syst Evol Microbiol 59, 472-476.
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