Supporting Information Legends
Figure S1. Public microarray data supports induction of the PEN1 PEN2 and PEN3 genes during biotic
stress. All available microarray experiments labeled as “biotic stress” were recovered from
GeneVestigator and the relative expression of PEN1, PEN2 and PEN3 was plotted for each
experiments. Only experiments conducted in Arabidopsis wild type were considered. Experiments
were sorted based on pathogen; Pseudomonas sp. (a), Fungi (b), Oomycete (c), Miscellaneous (d).
Figure S2. Independent T-DNA insertion lines display similar conductivity to that of point mutations
in the PEN genes. The location of the T-DNAs in the PEN genes is indicated. (a). Leaf discs were
prepared from the indicated Arabidopsis lines and infiltrated with Pst expressing AvrRpm1 (b). The
discs were incubated in water and the conductivity of the bathing solution was measured at the time
points indicated. Shown is mean and standard deviation of three replicates. Letters A and B at right
indicates experimental groups with significant differences (p < 0.05, ANOVA with Tukey’s post hoc
test).
Figure S3. Pen double and triple mutant combinations display chlorotic leaf tips and stunted growth
during aging. Plants were cultivated under short day conditions and photographed at indicated times.
The extent of chlorotic lesions was found to vary in different chambers and with age (a). Levels of
salicylic acid in plants tissue were measured at 5 and 8 weeks (b). Shown are averages and standard
deviation of 3 independent biological replicates. The experiment was performed twice with similar
results. Asterisk (*) indicates significant difference to Col-0 (p < 0.05, ANOVA with Tukey’s post hoc
test).
Figure S4. Indole glucosinolate biosynthetic network. Schematic overview of the indole glucosinolate
biosynthetic network adapted from (Bednarek, et al. 2009, Bones and Rossiter 1996, Clay, et al. 2009,
Kanehisa et al. 2012). Proteins encoded by genes investigated in this study (white boxes) and
proteins known but not assays in this study (black boxes). Dashed line indicates the unstable
thiohydroxamate-O-sulfonate intermediate.
Figure S5. The pen2 and cyp79B2/B3 mutants displays similar loss of cell death induced by
recognition of AvrRpm1 secreted by Pst DC3000. Leaf discs were prepared from the indicated
Arabidopsis lines were infiltrated with Pst DC3000 expressing AvrRpm1. The discs were incubated in
water and the conductivity of the bathing solution was measured at the time points indicated.
Shown is mean and standard deviation of six replicates. The experiment was repeated twice with
similar results. Letters A and B at right indicates experimental groups with significant differences (p <
0.05, ANOVA with Tukey’s post hoc test).
Table S1. Mutant lines used in this study.
Table S2. PCR based markers used for genotyping of pen mutant lines.