Supplementary Material for Review 2 - Progress curve
measurements for the identification of model parameters
Progress curves of glucose dehydrogenase (GDH) with NAD as cofactor
Figure A1: Progress curves of GDH with glucose and NAD as substrates. Triplicate
measurements of experiments 1-5 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Figure A2: Progress curves of GDH with glucose and NAD as substrates. Triplicate
measurements of experiments 6-12 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Figure A3: Progress curves of GDH with glucose and NAD as substrates. Triplicate
measurements of experiments 13-18 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Progress curves of glucose dehydrogenase (GDH) with NADP as cofactor
Figure A4: Progress curves of GDH with glucose and NADP as substrates. Triplicate
measurements of experiments 1-6 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Figure A5: Progress curves of GDH with glucose and NADP as substrates. Triplicate
measurements of experiments 7-12 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Figure A6: Progress curves of GDH with glucose and NADP as substrates. Triplicate
measurements of experiments 13-18 are shown. Experimental data are shown as empty circles,
simulated reactions courses with fitted parameters are shown as solid lines. Reactions were
conducted in 250 µL scale in microtiter plates at 30 °C and pH 7.0.
Progress curves of 3α-hydroxysteroid dehydrogenase (3α-HSDH)
Figure A7: Progress curves of 3α-HSDH with DHCA and NADH as substrates and 7,12diketo-UDCA and NAD as products. Duplicate measurements of experiments 1-12 are shown.
Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.
Figure A8: Progress curves of 3α-HSDH with DHCA and NADH as substrates and 7,12diketo-UDCA and NAD as products. Duplicate measurements of experiments 13-26 are
shown. Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.
Figure A9: Progress curves of 3α-HSDH with DHCA and NADH as substrates and 7,12diketo-UDCA and NAD as products. Duplicate measurements of experiments 27-40 are
shown. Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.
Progress curves of 7β-hydroxysteroid dehydrogenase (7β-HSDH)
Figure A10: Progress curves of 7β-HSDH with DHCA and NADPH as substrates and 3,12diketo-UDCA and NADP as products. Duplicate measurements of experiments 1-12 are
shown. Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.
Figure A11: Progress curves of 7β-HSDH with DHCA and NADPH as substrates and 3,12diketo-UDCA and NADP as products. Duplicate measurements of experiments 13-26 are
shown. Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.
Figure A12: Progress curves of 7β-HSDH with DHCA and NADPH as substrates and 3,12diketo-UDCA and NADP as products. Duplicate measurements of experiments 27-40 are
shown. Experimental data are shown as empty circles, simulated reactions courses with fitted
parameters are shown as solid lines. Reactions were conducted in 250 µL scale in microtiter
plates at 30 °C and pH 7.0.