Table S1. Oligonucleotides used in this study
Primer paira,b,c
Sequence (5’-3’)
Application
attB2-UP-citZ-F
GGGGACCACTTTGTACAAGAAAGCTGGGT
TAGCGAATATGCCGTGTTCA
and
Phos/AGTCTCCGCTGCGATAACC
Amplification
of
sequence upstream
of
citZ
for
recombination with
pKOR1
Amplification
of
sequence
downstream of citZ
for recombination
with pKOR1
Amplification
of
sequence upstream
of
sbnG
for
recombination with
pKOR1
Amplification
of
sequence
downstream of sbnG
for recombination
with pKOR1
Complementation of
citZ in pALC2073
and
UP-citZ-R
DOWN-citZ-F
and
attB1-DOWN-citZ-R
CCAATCTTTGCTGTAAGTCGTTC
and
GGGGACAAGTTTGTACAAAAAAGCAGGCT
CACCATTCAAGTTCATAGTTGCTAC
attB1-UP-sbnG-F
GGGGACAAGTTTGTACAAAAAAGCAGGCT
GAACACTTCAACGAAACGAG
and
Phos/CTATGTTTCAGAGATAGTTGTTGCAT
C
CCATTTAAGTGCATCTCTAGCGAC
and
GGGGACCACTTTGTACAAGAAAGCTGGGT
GCACTTCTGTCACATAGTAACC
and
UP-sbnG-R
DOWN-sbnG-F
and
attB2-DOWN-sbnG-R
citZ-F-SacI
and
citZ-R-SacI
sbnG-F-KpnI
and
sbnG-R-SacI
sfaA-749-F
and
sfa-941-R
sfaD-1262-F
and
sfaD-1450-R
sbnG-242-F
and
sbnG-422-R
rpoB-76-F
and
rpoB-232-R
GACTGAGCTCATGGCAGAATTACAAAGAG
and
GACTGAGCTCTGATTATTTTCTTTCTTCAAG
CG
TTGAGAGGTACCAAATCCATTAGGTGTAGG
AGGT
and
TTGAGAGAGCTCTGGATGAGATTGTGCTTT
TAAT
TGACGATGTGTGCCATTGGTG
and
CGTGCCCATTGACTAAATAACCC
AAAATGATTCCACAAGAAGTCACACC
and
GGATTACGAGACCAAGCAATGC
TCATTAAAGTGTTGGATATGGGTGC
and
TTAGCCATCTCCATTGCATCAAG
AGAGAAAGACGGCACTGAAAACAC
and
ATAACGACCCACGCTTGCTAAG
Complementation of
sbnG in pALC2073
(pJC10)
RT-PCR of sfaA
RT-PCR of sfaD
RT-PCR of sbnG
For RT-PCR of the
housekeeping gene
rpoB
a. Sequences in bold denote the attB1 or attB2 sequences for site-specific
recombination with pKOR1.
b. Phos/ denotes a 5’ phosphate on the primer.
c. Restriction sites for cloning are underlined.