Supporting Information 1. Table S1. The genes required for H2O2 tolerance (the value of (SM/SW)/(NM/NW) < 0.5 and p-value < 0.05).
Systematic
Name
Standard
H2O2
Name
sensitivity
p-value
YIL033C
YBL069W
YDR033W
BCY1
AST1
MRH1
0.029
0.030
0.038
9.39E-14
6.97E-12
2.94E-15
Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA)
Lipid raft associated protein
Protein that localizes primarily to the plasma membrane
NO
NO
NO
YGL006W
YBL021C
PMC1
HAP3
0.041
0.047
6.88E-14
5.13E-12
NO
NO
YBL075C
YBL005W
YDR507C
YPL143W
SSA3
PDR3
GIN4
RPL33A
0.050
0.055
0.062
0.065
2.68E-13
1.06E-14
5.50E-10
1.10E-13
Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions
Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p
CCAAT-binding complex
ATPase involved in protein folding and the response to stress
Transcriptional activator of the pleiotropic drug resistance network
Protein kinase involved in bud growth and assembly of the septin ring
Ribosomal 60S subunit protein L33A
NO
NO
NO
YES
YGL232W
YBL001C
YGR209C
YER109C
YPL235W
YGR289C
YLR293C
TAN1
ECM15
TRX2
FLO8
RVB2
MAL11
GSP1
0.069
0.071
0.078
0.083
0.092
0.095
0.126
5.84E-09
6.96E-10
4.35E-11
4.67E-08
5.79E-09
1.32E-09
4.41E-07
Putative tRNA acetyltransferase
on-essential protein of unknown function
Cytoplasmic thioredoxin isoenzyme
Transcription factor
ATP-dependent DNA helicase
Inducible high-affinity maltose transporter (alpha-glucoside transporter)
Ran GTPase
NO
NO
NO
NO
YES
NO
YES
YDL193W
YEL007W
YBL014C
YPR181C
YDR300C
NUS1
MIT1
RRN6
SEC23
PRO1
0.133
0.144
0.145
0.160
0.163
1.01E-05
8.78E-09
9.00E-07
1.51E-07
1.10E-08
Putative prenyltransferase
Transcriptional regulator of pseudohyphal growth
Component of the core factor (CF) rDNA transcription factor complex
GTPase-activating protein
Gamma-glutamyl kinase
YES
NO
YES
YES
NO
Description
Essential
gene
YDL191W
YMR053C
YBL047C
YDR353W
YBR223C
YNL166C
RPL35A
STB2
EDE1
TRR1
TDP1
BNI5
0.164
0.176
0.176
0.178
0.178
0.187
3.51E-09
1.81E-05
1.31E-07
8.02E-07
2.70E-09
4.16E-07
Ribosomal 60S subunit protein L35A
Protein that interacts with Sin3p in a two-hybrid assay
Endocytic protei
Cytoplasmic thioredoxin reductase
Tyrosyl-DNA phosphodiesterase I
Protein involved in organization of septins at the mother-bud neck
NO
NO
NO
YES
NO
NO
YPR036W
VMA13
0.194
3.55E-05
NO
YEL036C
YLR287C-A
YGL070C
YLR286C
YEL026W
ANP1
RPS30A
RPB9
CTS1
SNU13
0.202
0.213
0.213
0.221
0.227
3.70E-07
1.47E-07
6.24E-07
6.72E-06
4.72E-08
Subunit H of the eight-subunit V1 peripheral membrane domain of the
vacuolar H+-ATPase (V-ATPase)
Subunit of the alpha-1,6 mannosyltransferase complex
Protein component of the small (40S) ribosomal subunit
RNA polymerase II subunit B12.
Endochitinase
RNA binding protein
YDR077W
YDR404C
YPR187W
YIL041W
SED1
RPB7
RPO26
GVP36
0.228
0.235
0.242
0.251
6.99E-05
5.63E-07
8.11E-07
2.41E-07
YLR312W-A
YDL043C
MRPL15
PRP11
0.253
0.256
YML028W
YJL102W
YMR013C
YGR255C
YOL159C
TSA1
MEF2
SEC59
COQ6
0.259
0.259
0.261
0.265
0.269
NO
NO
NO
NO
YES
NO
YES
YES
NO
1.58E-06
8.35E-08
Major stress-induced structural GPI-cell wall glycoprotein
RNA polymerase II subunit B16
RNA polymerase subunit ABC23
BAR domain-containing protein that localizes to both early and late
Golgi vesicles
Mitochondrial ribosomal protein of the large subunit
Subunit of the SF3a splicing factor complex
6.60E-08
2.99E-08
1.08E-05
1.88E-06
3.72E-04
Thioredoxin peroxidase
Mitochondrial elongation factor involved in translational elongation
Dolichol kinase
Putative flavin-dependent monooxygenase
Soluble protein of unknown function
NO
NO
YES
NO
NO
NO
YES
YEL063C
YLR312C
YMR198W
YLR292C
CAN1
CIK1
SEC72
0.269
0.281
0.283
0.284
1.47E-05
2.55E-05
6.42E-05
4.65E-06
NO
NO
NO
NO
2.76E-07
Plasma membrane arginine permease
Putative protein of unknown function
Kinesin-associated protein
Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and
Sec72p)
Plasma membrane riboflavin transporter
YOR306C
MCH5
0.293
YEL027W
YEL035C
YEL050C
YDR388W
YCR076C
YBR020W
YBL091C
VMA3
UTR5
RML2
RVS167
FUB1
GAL1
MAP2
0.298
0.302
0.303
0.308
0.310
0.310
0.311
2.82E-05
3.74E-08
1.69E-04
5.68E-07
4.83E-06
3.44E-04
1.13E-04
Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c)
Protein of unknown function
Mitochondrial ribosomal protein of the large subunit
Actin-associated protein with roles in endocytosis and exocytosis
Putative protein of unknown function
Galactokinase
Methionine aminopeptidase
NO
YES
NO
NO
NO
NO
NO
YLR297W
YJR151W-A
YLR289W
GUF1
0.313
0.316
0.318
3.35E-06
4.36E-07
9.80E-08
NO
NO
NO
YIL046W
YLR300W
YNL169C
MET30
EXG1
PSD1
0.320
0.321
0.323
4.50E-08
2.16E-07
5.79E-04
Putative protein of unknown function
Protein of unknown function
Mitochondrial matrix GTPase that associates with mitochondrial
ribosomes
F-box protein containing five copies of the WD40 motif
Major exo-1,3-beta-glucanase of the cell wall
Phosphatidylserine decarboxylase of the mitochondrial inner membrane
YES
NO
NO
YIL076W
YDR156W
YDR145W
YGL043W
YFL005W
SEC28
RPA14
TAF12
DST1
SEC4
0.323
0.324
0.325
0.327
0.327
4.40E-06
1.05E-05
8.85E-04
1.39E-04
1.56E-08
Epsilon-COP subunit of the coatomer
RNA polymerase I subunit A14
Subunit (61/68 kDa) of TFIID and SAGA complexes
General transcription elongation factor TFIIS
Rab family GTPase essential for vesicle-mediated exocytic secretion and
NO
NO
YES
NO
YES
NO
YCR045C
RRT12
0.328
2.66E-04
YGL030W
YDR350C
YLR295C
RPL30
ATP22
ATP14
0.330
0.334
0.336
5.59E-07
1.22E-04
6.95E-07
autophagy
Probable subtilisin-family protease with a role in formation of the
dityrosine layer of spore walls
Ribosomal 60S subunit protein L30
Specific translational activator for the mitochondrial ATP6 mRNA
Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase
YAL005C
YJL146W
YLR284C
YDR132C
YHR010W
YDR212W
YEL020C
SSA1
IDS2
ECI1
0.338
0.339
0.340
0.341
0.344
0.344
0.344
8.63E-07
9.34E-07
1.44E-08
5.77E-04
2.73E-05
5.69E-06
2.77E-04
ATPase involved in protein folding and NLS-directed nuclear transport
Protein involved in modulation of Ime2p activity during meiosis
Peroxisomal delta3,delta2-enoyl-CoA isomerase
Putative protein of unknown function
Ribosomal 60S subunit protein L27A
Alpha subunit of chaperonin-containing T-complex
Protein of unknown function with low sequence identity to Pdc1p
NO
NO
NO
NO
NO
YES
NO
YDR362C
TFC6
0.345
6.23E-05
YES
YMR309C
YLR299W
YDR349C
YIL018W
YGL068W
NIP1
ECM38
YPS7
RPL2B
MNP1
0.346
0.348
0.349
0.351
0.351
1.03E-04
1.74E-03
5.65E-05
2.52E-07
6.17E-05
One of six subunits of RNA polymerase III transcription initiation factor
complex (TFIIIC)
eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3)
Gamma-glutamyltranspeptidase
Putative GPI-anchored aspartic protease
Ribosomal 60S subunit protein L2B
Protein associated with the mitochondrial nucleoid
YGR216C
YOR163W
YPL131W
YEL017W
GPI1
DDP1
RPL5
GTT3
0.352
0.354
0.356
0.357
8.50E-05
3.16E-03
3.99E-04
5.32E-09
Membrane protein
Polyphosphate phosphatase
Ribosomal 60S subunit protein L5
Protein of unknown function with a possible role in glutathione
metabolism
YES
NO
YES
NO
RPL27A
TCP1
NO
YES
NO
NO
YES
NO
NO
NO
YES
YBL011W
SCT1
0.357
3.03E-06
1.35E-06
9.89E-07
4.28E-05
Glycerol 3-phosphate/dihydroxyacetone phosphate dual
substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis
pathway
Trichostatin A-insensitive homodimeric histone deacetylase (HDAC)
Central kinetochore protein and subunit of the Ctf19 complex
Protein component of the small (40S) ribosomal subunit
YPL116W
YLR315W
YOL040C
HOS3
NKP2
RPS15
0.358
0.359
0.361
YEL015W
YGL071W
YGR057C
YDR254W
YEL013W
YDR374C
YDR363W
EDC3
AFT1
LST7
CHL4
VAC8
NO
NO
YES
ESC2
0.362
0.364
0.365
0.365
0.365
0.367
0.369
1.24E-07
1.29E-03
2.84E-04
6.83E-05
1.48E-06
2.38E-05
1.17E-03
Non-essential conserved protein with a role in mRNA decapping
Transcription factor involved in iron utilization and homeostasis
Protein possibly involved in a post-Golgi secretory pathway
Outer kinetochore protein required for chromosome stability
Phosphorylated and palmitoylated vacuolar membrane protein
Putative protein of unknown function
Sumo-like domain protein
NO
NO
NO
NO
NO
NO
NO
YMR002W
YDR373W
YGL056C
YGR257C
YDR320C
YOR151C
YDR493W
MIC17
FRQ1
SDS23
MTM1
SWA2
RPB2
MZM1
0.371
0.371
0.373
0.376
0.377
0.378
0.380
3.51E-05
4.01E-03
5.55E-04
9.11E-03
1.20E-03
1.42E-05
2.21E-04
Mitochondrial intermembrane space protein
N-myristoylated calcium-binding protein
Protein involved in cell separation during budding
Mitochondrial protein of the mitochondrial carrier family
Auxilin-like protein involved in vesicular transport
RNA polymerase II second largest subunit B150
Protein required for assembly of the cytochrome bc(1) complex
NO
YES
NO
NO
NO
YES
NO
YPR086W
YDR144C
YOR149C
YOR338W
YDR389W
SUA7
MKC7
SMP3
0.381
0.381
0.381
0.382
0.382
4.57E-06
2.13E-03
6.34E-04
4.41E-06
1.01E-06
Transcription factor TFIIB
GPI-anchored aspartyl protease
Alpha 1,2-mannosyltransferase
Putative protein of unknown function
GTPase activating protein (GAP) for Rho1p
YES
NO
YES
NO
NO
SAC7
NO
YJL006C
YDR375C
YPL124W
YOR103C
CTK2
BCS1
SPC29
OST2
0.385
0.385
0.385
0.385
8.87E-05
9.71E-06
5.97E-06
1.23E-06
YKL189W
HYM1
0.387
YHR075C
YPR133W-A
YNL331C
YJL005W
YHR206W
YJR005W
YIL045W
PPE1
TOM5
AAD14
CYR1
SKN7
APL1
PIG2
YDR357C
YKL057C
YLR313C
YDR358W
YKL165C
YDR345C
YJL179W
YCR051W
YJR083C
YEL046C
YDR135C
YKR048C
NO
NO
YES
YES
7.29E-03
Beta subunit of C-terminal domain kinase I (CTDK-I)
Protein translocase and chaperone required for Complex III assembly
Inner plaque spindle pole body (SPB) component
Epsilon subunit of the oligosaccharyltransferase complex of the ER
lumen
Component of the RAM signaling network
0.387
0.388
0.389
0.389
0.389
0.390
0.390
5.49E-09
3.45E-05
1.81E-04
1.85E-04
4.44E-08
1.46E-07
7.23E-08
Protein with carboxyl methyl esterase activity
Component of the TOM (translocase of outer membrane) complex
Putative aryl-alcohol dehydrogenase
Adenylate cyclase
Nuclear response regulator and transcription factor
Beta-adaptin
Putative type-1 protein phosphatase targeting subunit
NO
NO
NO
YES
NO
NO
NO
CNL1
NUP120
SPH1
GGA1
MCD4
HXT3
PFD1
0.390
0.390
0.391
0.391
0.391
0.392
0.392
1.02E-05
4.89E-03
9.85E-05
9.87E-05
9.82E-03
1.54E-03
1.01E-08
Protein of unknown function
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)
Protein involved in shmoo formation and bipolar bud site selection
Golgi-localized protein with homology to gamma-adaptin
Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis
Low affinity glucose transporter of the major facilitator superfamily
Subunit of heterohexameric prefoldin
NO
NO
NO
NO
YES
NO
NO
ACF4
GLY1
YCF1
NAP1
0.393
0.393
0.394
0.394
0.395
1.74E-07
1.95E-05
6.87E-05
2.68E-05
5.12E-04
Protein of unknown function
Protein of unknown function
Threonine aldolase
Vacuolar glutathione S-conjugate transporter
Histone chaperone
NO
NO
NO
NO
NO
YES
YEL030W
YOL061W
YBL086C
YLR074C
YML007W
YLR016C
ECM10
PRS5
0.395
0.396
0.397
0.397
0.398
0.398
1.29E-05
5.84E-06
4.39E-08
7.44E-05
1.08E-03
7.55E-07
Heat shock protein of the Hsp70 family
5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase
Protein of unknown function
Protein involved in bud-site selection
Basic leucine zipper (bZIP) transcription factor
Subunit of the RES complex
NO
NO
NO
NO
NO
NO
YDR365C
YEL023C
YEL001C
YCR107W
YIL005W
YML002W
YOR202W
ESF1
HIS3
0.398
0.399
0.399
0.400
0.401
0.401
0.402
1.12E-07
4.62E-07
6.71E-05
1.72E-06
7.00E-04
1.23E-04
1.71E-06
Nucleolar protein involved in pre-rRNA processing
Protein of unknown function
Putative protein of unknown function
Putative aryl-alcohol dehydrogenase
ER protein with chaperone and co-chaperone activity
Putative protein of unknown function
Imidazoleglycerol-phosphate dehydratase
YES
NO
NO
NO
NO
NO
NO
YDR392W
SPT3
0.404
5.26E-09
NO
YEL024W
YPR104C
YDR425W
YLR287C
YIL064W
RIP1
FHL1
SNX41
0.404
0.405
0.407
0.407
0.408
8.42E-06
9.81E-10
3.02E-08
9.68E-06
1.74E-06
Subunit of the SAGA and SAGA-like transcriptional regulatory
complexes
Ubiquinol-cytochrome-c reductase
Regulator of ribosomal protein (RP) transcription
Sorting nexin
Putative protein of unknown function
Probable lysine methyltransferase involved in the dimethylation of
NO
YES
NO
NO
NO
8.80E-04
3.98E-07
7.73E-08
7.70E-09
eEF1A (Tef1p/Tef2p)
Mitochondrial ribosomal protein of the small subunit
Protein with ubiquitin-like N terminus
Putative protein of unknown function
Transverse filament protein of the synaptonemal complex
NO
NO
NO
NO
YDR347W
YEL037C
YLR283W
YDR285W
BUD20
YAP1
PML1
IRC22
AAD3
EPS1
SEE1
MRP1
RAD23
ZIP1
0.408
0.408
0.409
0.411
YCR082W
YEL017C-A
YJL170C
YBR191W
YLL058W
YCR050C
AHC2
PMP2
ASG7
RPL21A
0.411
0.411
0.411
0.412
0.412
0.412
7.34E-05
2.18E-05
8.42E-04
7.69E-06
2.49E-05
1.28E-05
Component of the ADA histone acetyltransferase complex
Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p)
Protein that regulates signaling from a G protein beta subunit Ste4p
Ribosomal 60S subunit protein L21A
Putative protein of unknown function with similarity to Str2p
Non-essential protein of unknown function
NO
NO
NO
NO
NO
NO
YGR162W
YCR077C
YOR026W
YBL051C
TIF4631
PAT1
BUB3
PIN4
0.412
0.413
0.413
0.413
7.79E-05
2.51E-09
3.28E-03
1.59E-04
NO
NO
NO
NO
YHR128W
YGR042W
FUR1
0.414
0.414
1.60E-07
1.67E-03
Translation initiation factor eIF4G
Deadenylation-dependent mRNA-decapping factor
Kinetochore checkpoint WD40 repeat protein
Protein involved in G2/M phase progression and response to DNA
damage
Uracil phosphoribosyltransferase
Putative protein of unknown function
YES
NO
YDR289C
YDR119W
YJL186W
YDR361C
YDR348C
YML073C
YPR021C
RTT103
VBA4
MNN5
BCP1
PAL1
RPL6A
AGC1
0.416
0.417
0.417
0.417
0.418
0.418
0.418
1.28E-05
4.37E-03
3.95E-06
7.55E-05
7.34E-05
1.23E-03
6.99E-09
Protein that interacts with exonuclease Rat1p and Rai1p
Protein of unknown function
Alpha-1,2-mannosyltransferase
Essential protein involved in nuclear export of Mss4p
Protein of unknown function thought to be involved in endocytosis
Ribosomal 60S subunit protein L6A
Mitochondrial amino acid transporter
NO
NO
NO
YES
NO
NO
NO
YLR163C
YGR071C
YDR257C
YJR079W
YOR127W
MAS1
ENV11
RKM4
0.418
0.420
0.420
0.420
0.421
4.15E-03
1.49E-03
2.49E-04
7.18E-05
3.74E-05
Smaller subunit of the mitochondrial processing protease (MPP)
Protein proposed to be involved in vacuolar functions
Ribosomal lysine methyltransferase
Protein of unknown function
GTPase-activating protein for polarity-establishment protein Cdc42p
YES
NO
NO
NO
NO
RGA1
YML012W
ERV25
0.422
4.36E-05
YDR364C
YDR261C
YDR121W
CDC40
EXG2
DPB4
0.422
0.422
0.422
2.69E-07
2.65E-07
2.86E-05
YKL190W
YKL167C
YIL048W
YDR338C
YOR335C
YDR120C
YDR387C
CNB1
MRP49
NEO1
0.423
0.423
0.423
0.424
0.424
0.424
0.425
YDR352W
YNL330C
YOR324C
YCR071C
YDR376W
YJL020C
YLR288C
YPQ2
RPD3
FRT1
IMG2
ARH1
BBC1
MEC3
YDR064W
YCR086W
YDL211C
YOR307C
YLR388W
RPS13
CSM1
ALA1
TRM1
SLY41
RPS29A
Protein that forms a heterotrimeric complex with Erp1, Erp2p, and
Emp24, member of the p24 family
Pre-mRNA splicing factor
Exo-1,3-beta-glucanase
Subunit of DNA pol epsilon and of ISW2 chromatin accessibility
complex
NO
3.09E-04
1.32E-02
4.21E-06
2.03E-03
2.46E-09
2.90E-04
3.25E-04
Calcineurin B
Mitochondrial ribosomal protein of the large subunit
Putative aminophospholipid translocase (flippase)
Putative protein of unknown function
Cytoplasmic and mitochondrial alanyl-tRNA synthetase
tRNA methyltransferase
Putative transporter
NO
NO
YES
NO
YES
NO
NO
0.426
0.426
0.426
0.427
0.428
0.428
0.429
7.88E-03
4.48E-10
3.54E-04
2.12E-04
2.34E-03
4.58E-04
3.70E-04
Putative vacuolar membrane transporter for cationic amino acids
Histone deacetylase
Tail-anchored ER membrane protein of unknown function
Mitochondrial ribosomal protein of the large subunit
Oxidoreductase of the mitochondrial inner membrane
Protein possibly involved in assembly of actin patches
DNA damage and meiotic pachytene checkpoint protein
NO
NO
NO
NO
YES
NO
NO
0.429
0.429
0.430
0.430
0.430
7.93E-06
6.51E-04
3.75E-05
1.35E-06
6.30E-04
Protein component of the small (40S) ribosomal subuni
Nucleolar protein
Protein of unknown function
Protein involved in ER-to-Golgi transport
Protein component of the small (40S) ribosomal subunit
YES
NO
NO
NO
NO
NO
NO
NO
YDR359C
YPL272C
YGR186W
YFR055W
YOR293W
YDR351W
EAF1
TFG1
IRC7
RPS10A
SBE2
0.430
0.431
0.431
0.433
0.433
0.434
1.07E-04
4.94E-05
2.33E-06
1.05E-10
2.94E-05
2.62E-03
Component of the NuA4 histone acetyltransferase complex
Putative protein of unknown function
TFIIF (Transcription Factor II) largest subunit
Beta-lyase involved in the production of thiols
Protein component of the small (40S) ribosomal subunit
Protein required for bud growth
NO
NO
YES
NO
NO
NO
YGL081W
YAR002W
NUP60
0.436
0.436
3.32E-03
8.11E-06
NO
NO
YGL097W
YDR372C
YDR143C
YEL065W
SRM1
VPS74
SAN1
SIT1
0.437
0.437
0.439
0.439
3.70E-07
4.79E-05
2.54E-03
3.63E-07
Putative protein of unknown function
FG-nucleoporin component of central core of the nuclear pore complex
(NPC)
Nucleotide exchange factor for Gsp1p
Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor
Ubiquitin-protein ligase
Ferrioxamine B transporter
YES
NO
NO
NO
YMR258C
YDR299W
YJL176C
YFL010W-A
YCR073W-A
YDR318W
YEL016C
ROY1
BFR2
SWI3
AUA1
SOL2
MCM21
NPP2
0.440
0.440
0.440
0.440
0.441
0.441
0.441
1.10E-09
9.89E-06
1.39E-09
7.93E-08
2.35E-04
6.57E-06
5.09E-07
GTPase inhibitor with similarity to F-box proteins
Essential protein that is a component of 90S preribosomes
Subunit of the SWI/SNF chromatin remodeling complex
Protein required for the negative regulation by ammonia of Gap1p
Protein with a possible role in tRNA export
Component of the kinetochore sub-complex COMA
Nucleotide pyrophosphatase/phosphodiesterase family member
NO
YES
NO
NO
NO
NO
NO
YGR055W
YDR354W
MUP1
TRP4
0.441
0.442
3.75E-03
1.17E-10
NO
NO
YBL087C
YFL004W
RPL23A
VTC2
0.442
0.443
1.33E-04
4.74E-05
High affinity methionine permease
Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic
pathway
Ribosomal 60S subunit protein L23A
Subunit of vacuolar transporter chaperone (VTC) complex
NO
NO
YEL044W
IES6
0.444
4.23E-10
YLR306W
YDR131C
YMR266W
YDR512C
UBC12
0.444
0.445
0.446
0.446
7.30E-04
2.04E-02
4.86E-04
1.20E-05
RSN1
EMI1
YLR131C
YPR089W
YCL064C
YDR390C
ACE2
Protein that associates with the INO80 chromatin remodeling complex
under low-salt conditions
Enzyme that mediates the conjugation of Rub1p
F-box protein
Membrane protein of unknown function
Non-essential protein required for transcriptional induction of the early
meiotic-specific transcription factor IME1
Transcription factor required for septum destruction after cytokinesis
Protein of unknown function
Catabolic L-serine (L-threonine) deaminase
Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with
Aos1p
Transcriptional modulator
CHA1
UBA2
0.447
0.447
0.447
0.449
3.15E-03
1.24E-06
4.23E-06
1.65E-09
YAL013W
DEP1
0.449
3.03E-04
YGR113W
YMR014W
DAM1
BUD22
0.449
0.449
7.55E-04
8.83E-04
YJL010C
YNL103W
YER028C
YOR319W
NOP9
MET4
MIG3
HSH49
0.449
0.450
0.452
0.452
6.37E-05
9.72E-11
2.09E-07
3.29E-07
Essential subunit of the Dam1 complex (aka DASH complex)
Protein required for 18S rRNA maturation and small ribosomal subunit
biogenesis
Essential subunit of U3-containing 90S preribosome
Leucine-zipper transcriptional activator
Transcriptional regulator
U2-snRNP associated splicing factor with similarity to the mammalian
1.27E-07
1.00E-04
1.38E-03
4.08E-06
splicing factor SAP49
Protein component of the small (40S) ribosomal subunit
Ubiquitin-conjugating enzyme
Component of the RSC chromatin remodeling complex
Mitochondrial membrane localized inositol phosphosphingolipid
YOR096W
YOR339C
YGR056W
YER019W
RPS7A
UBC11
RSC1
ISC1
0.453
0.453
0.453
0.454
NO
NO
NO
NO
NO
NO
NO
NO
YES
NO
YES
NO
YES
YES
NO
YES
NO
NO
NO
NO
YDR275W
YGR041W
YCR044C
YOR316C
YGL082W
BSC2
BUD9
PER1
COT1
0.455
0.457
0.457
0.457
0.458
1.67E-03
1.76E-03
2.64E-03
1.73E-04
2.63E-03
phospholipase C
Protein of unknown function
Protein involved in bud-site selection
Protein of the endoplasmic reticulum
Vacuolar transporter that mediates zinc transport into the vacuole
Putative protein of unknown function
YGR070W
YDR140W
YOR100C
YIL073C
YNL220W
YOR116C
YJL130C
ROM1
MTQ2
CRC1
SPO22
ADE12
RPO31
URA2
0.459
0.459
0.460
0.460
0.460
0.461
0.461
3.49E-03
1.33E-04
1.88E-05
7.83E-05
1.57E-06
9.43E-04
1.26E-03
GDP/GTP exchange protein (GEP) for Rho1p
S-adenosylmethionine-dependent methyltransferase
Mitochondrial inner membrane carnitine transporter
Meiosis-specific protein essential for chromosome synapsis
Adenylosuccinate synthase
RNA polymerase III largest subunit C160
Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate
RRP4
BAR1
0.462
0.462
0.462
7.00E-07
3.37E-06
5.56E-03
YJL201W
YGR258C
ECM25
RAD2
0.463
0.463
7.63E-06
1.05E-08
transcarbamylase (ATCase)
Protein of unknown function
Exosome non-catalytic core component
Aspartyl protease secreted into the periplasmic space of mating type a
cells
Non-essential protein of unknown function
Single-stranded DNA endonuclease
YNL313C
YML013W
YDR436W
YGR254W
YDL031W
EMW1
UBX2
PPZ2
ENO1
DBP10
0.465
0.465
0.466
0.466
0.466
8.66E-07
8.93E-04
2.54E-13
9.36E-06
7.81E-04
Essential conserved protein with a role in cell wall integrity
Bridging factor involved in ER-associated protein degradation (ERAD)
Serine/threonine protein phosphatase Z
Enolase I
Putative ATP-dependent RNA helicase of the DEAD-box protein family
YLR290C
YHR069C
YIL015W
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
YES
NO
NO
YES
NO
NO
NO
YES
NO
NO
NO
YES
YEL051W
VMA8
0.466
3.08E-09
YDR418W
YOL127W
YBL093C
YIL011W
RPL12B
RPL25
ROX3
TIR3
0.467
0.467
0.467
0.467
3.50E-03
4.81E-04
2.22E-04
3.38E-07
YKL154W
YML003W
YDR416W
YPR141C
SRP102
SYF1
KAR3
0.468
0.469
0.469
0.470
1.90E-02
5.96E-05
1.24E-06
1.30E-05
YDR288W
YBL063W
NSE3
KIP1
0.470
0.471
1.07E-04
2.49E-04
YBR189W
YDR378C
YCR023C
YOR292C
YNL240C
RPS9B
LSM6
NAR1
0.471
0.471
0.472
0.472
0.473
3.37E-05
5.59E-05
8.51E-04
9.22E-05
3.73E-03
YKL008C
LAC1
0.473
YOR340C
YJL189W
YMR296C
YEL025C
YDR423C
RPA43
RPL39
LCB1
0.474
0.475
0.476
0.476
0.476
CAD1
Subunit D of the eight-subunit V1 peripheral membrane domain of the
vacuolar H+-ATPase (V-ATPase)
Ribosomal 60S subunit protein L12B
Ribosomal 60S subunit protein L25
Subunit of the RNA polymerase II mediator complex
Cell wall mannoprotein
NO
NO
YES
NO
NO
Signal recognition particle (SRP) receptor beta subunit
Putative protein of unknown function
Member of the NineTeen Complex (NTC)
Minus-end-directed microtubule motor that functions in mitosis and
meiosis
Component of the SMC5-SMC6 complex
Kinesin-related motor protein required for mitotic spindle assembly
YES
NO
YES
NO
NO
NO
NO
NO
YES
4.53E-05
Protein component of the small (40S) ribosomal subunit
Lsm (Like Sm) protein
Vacuolar membrane protein of unknown function
Protein of unknown function
Component of the cytosolic iron-sulfur (FeS) protein assembly
machinery
Ceramide synthase component
4.65E-07
3.09E-04
7.16E-07
1.31E-03
7.49E-05
RNA polymerase I subunit A43
Ribosomal 60S subunit protein L39
Component of serine palmitoyltransferase
Putative protein of unknown function
AP-1-like basic leucine zipper (bZIP) transcriptional activator
YES
NO
YES
NO
NO
YES
NO
NO
YDR146C
YKL178C
YDR341C
YJL103C
YFL003C
YNL312W
SWI5
STE3
RRS1
GSM1
MSH4
RFA2
0.477
0.477
0.478
0.480
0.481
0.481
2.53E-03
2.82E-02
4.91E-05
2.64E-04
3.71E-04
1.69E-05
Transcription factor that recruits Mediator and Swi/Snf complexes
Receptor for a factor pheromone
Arginyl-tRNA synthetase
Putative zinc cluster protein of unknown function
Protein involved in meiotic recombination
Subunit of heterotrimeric Replication Protein A (RPA)
NO
NO
YES
NO
NO
YES
YKR078W
YDL058W
USO1
0.482
0.483
6.16E-08
1.79E-05
NO
YES
YCL061C
YLL057C
YEL003W
YDR182W
MRC1
JLP1
GIM4
CDC1
0.483
0.483
0.483
0.484
1.02E-08
1.42E-04
7.64E-07
8.10E-06
Cytoplasmic protein of unknown function
Essential protein involved in the vesicle-mediated ER to Golgi transport
step of secretion
S-phase checkpoint protein required for DNA replication
Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase
Subunit of the heterohexameric cochaperone prefoldin complex
Putative lipid phosphatase of the endoplasmic reticulum
SPS100
RPS7B
PEX18
NIC96
GNT1
0.485
0.486
0.486
0.488
0.488
0.490
0.490
1.93E-06
4.59E-03
1.40E-04
1.52E-02
8.51E-08
7.81E-03
1.09E-03
Protein of unknown function
Protein required for spore wall maturation
Protein component of the small (40S) ribosomal subunit
Peroxin
Linker nucleoporin component of the nuclear pore complex (NPC)
N-acetylglucosaminyltransferase
Putative protein of unknown function
NO
NO
NO
NO
YES
NO
NO
0.490
0.491
0.491
0.492
0.494
1.27E-07
2.35E-11
1.67E-02
1.28E-03
6.30E-05
Protein kinase primarily involved in telomere length regulation
Essential protein with similarity to Rpl24Ap and Rpl24Bp
Transferrin receptor-like protein
Putative protein of unknown function
Subunit of TRAPPII
NO
YES
NO
NO
NO
YOR289W
YHR139C
YNL096C
YHR160C
YFR002W
YOR320C
YOR152C
YBL088C
YLR009W
YPL176C
YGR111W
YEL048C
TEL1
RLP24
TRE1
TCA17
NO
NO
NO
YES
YDR498C
SEC20
0.494
1.08E-05
YOL151W
GRE2
0.494
1.28E-03
YFL007W
YCR011C
BLM10
ADP1
0.495
0.495
2.79E-03
5.83E-06
Membrane glycoprotein v-SNARE involved in retrograde transport from
the Golgi to the ER
3-methylbutanal reductase and NADPH-dependent methylglyoxal
reductase (D-lactaldehyde dehydrogenase)
Proteasome activator
Putative ATP-dependent permease of the ABC transporter family of
7.75E-03
5.30E-03
1.39E-02
4.67E-07
2.37E-06
4.71E-08
proteins
Microsomal cytochrome b reductase
Penta-EF-hand protein
Component of the cleavage and polyadenylation factor I (CF I)
Mitochondrial ribosomal protein of the large subunit
Protein of unknown function
Acetate transporter required for normal sporulation
NO
NO
YES
NO
NO
NO
4.78E-06
5.16E-11
2.64E-03
2.60E-06
2.32E-06
Phenylpyruvate decarboxylase
Putative mitochondrial NAD+ transporter
Strand exchange protein
Putative PINc domain nuclease
Telobox-containing general regulatory factor
NO
NO
NO
YES
YES
YIL043C
YGR058W
YGL044C
YOR150W
YDR391C
YCR010C
CBR1
PEF1
RNA15
MRPL23
ADY2
0.496
0.496
0.496
0.497
0.498
0.498
YDR380W
YEL006W
YER095W
YDR339C
YPL128C
ARO10
YEA6
RAD51
FCF1
TBF1
0.498
0.498
0.498
0.499
0.499
YES
NO
NO
NO