Table S1. Insect taxa (n = 344 OTUs) collected in prairies, pastures, and maize fields of Brookings County, SD; and sum of each OTU collected from all study sites (n = 9). Vouchers of all specimens are housed in the permanent collection of Dr. Jonathan Lundgren. Taxon Ant 1 Ant 2 Ant 3 Ant 4 Ant 5 Ant 6 Ant 7 Ant 8 Ant 9 Ant 10 Ant 11 Ant 12 Ant 13 Ant 14 Ant 15 Ant 16 Ant 17 Ant 18 Ant 19 Ant 20 Ant 21 Cricket 1 Cricket 2 Tree Cricket 1 Moth 1 Moth 2 Moth 3 Moth 4 Moth 5 Moth 6 Moth 7 Moth 8 Moth 9 Moth 10 Moth 11 Moth 12 Moth 13 Staph 1 Number of specimens 123 1 4 5 104 23 8 4 43 8 2 4 11 1 6 2 11 9 403 1 1 24 1 1 1 2 2 2 1 2 1 1 1 1 1 1 1 2 Staph 2 Staph 3 Staph 4 Staph 5 Staph 6 Staph 7 Staph 8 Staph 9 Pselaphinae (Staph) 1 Collembola: Entomobryomorpha 1 Collembola: Entomobryomorpha 2 Collembola: Sminthuridae 1 Collembola Sminthuridae 2 Collembola Sminthuridae 3 Psocoptera 1 Psocoptera 2 Psocoptera 3 Psocoptera 4 Psocoptera 5 Coleoptera ( LBB) 1 Coleoptera: Cucujidae (LBB) 2 Coleoptera (LBB) 3 Coleoptera (LBB) 4 Coleoptera (LBB) 5 Coleoptera (LBB) 6 Coleoptera (LBB) 7 Coleoptera (LBB) 8 Coleoptera (LBB) 9 Coleoptera (LBB) 10 Coleoptera: Cucujidae 11 Coleoptera: LBB 12 Coleoptera: LBB 13 Coleoptera: LBB 14 Coleoptera: Bruchidae 15 Coleoptera: Little brown beetle 16 Carabidae: Bembidion quadrimaculatum 1 Carabidae: Elaphropus 1 Carabidae: Microlestes 1 Carabidae: Polyderus 1 Carabidae: Stenolophus comma Unknown Carabidae 1 Unknown Carabidae 2 Unknown Carabidae 3 5 4 1 3 9 3 6 1 6 650 15 4 170 75 14 4 3 2 1 50 2 3 5 1 1 7 5 1 3 1 1 1 3 3 1 6 39 1 2 10 2 2 1 Unknown Carabidae 4 Unknown Carabidae 5 Unknown Carabidae 7 Unknown Carabidae 8 Unknown Carabidae 9 Leafhopper 1 Leafhopper 2 Leafhopper 3 Leafhopper 4 Leafhopper 5 Leafhopper 6 Leafhopper 7 Leafhopper 8 Leafhopper 9 Leafhopper 10 Leafhopper 11 Leafhopper 12 Leafhopper 13 Leafhopper 14 Leafhopper 15 Leafhopper 16 Leafhopper 17 Leafhopper 19 Leafhopper 20 Leafhopper 21 Leafhopper 22 Leafhopper 23 Leafhopper 24 Leafhopper 25 Leafhopper 26 Leafhopper 27 Leafhopper 28 Leafhopper 29 Leafhopper 30 Leafhopper 31 Leafhopper 32 Leafhopper 33 Leafhopper 34 Leafhopper 35 Leafhopper 36 Leafhopper 37 Leafhopper 38 Leafhopper 39 1 1 3 1 1 2 55 2 32 4 20 5 4 5 5 23 3 15 16 2 2 1 1 3 3 2 4 5 11 2 11 3 9 10 1 3 9 3 2 1 2 3 1 Leafhopper 40 Leafhopper 41 Leafhopper 42 Leafhopper 43 Leafhopper 44 Cydnidae 1 Shield Bug 1 Anthocoridae 1 Anthocoridae 2 Anthocoridae (Orius insidiosus) 3 Nabidae 1 Diptera 1 Diptera 2 Diptera 3 Diptera 4 Diptera 5 Diptera 6 Diptera 7 Diptera 8 Diptera 9 Diptera 10 Diptera 11 Diptera 13 Diptera 14 Diptera 15 Diptera 16 Diptera 17 Diptera 18 Diptera 19 Diptera 20 Diptera 21 Diptera 22 Diptera 23 Diptera 24 Diptera 25 Diptera 26 Diptera 27 Diptera 28 Diptera 29 Diptera 30 Diptera 31 Diptera 32 Diptera 33 1 1 1 2 1 1 2 1 1 22 47 30 3 3 11 12 2 4 3 12 1 5 3 70 11 3 20 10 4 8 6 1 3 2 1 1 9 1 9 3 2 6 3 Diptera 34 Diptera 35 Diptera 36 Diptera 37 Diptera 38 Diptera 39 Diptera 40 Diptera 41 Diptera 42 Diptera 43 Diptera 44 Diptera 45 Diptera 46 Diptera 47 Diptera 48 Diptera 49 Diptera 50 Diptera 51 Parasitic Hymenoptera 1 Parasitic Hymenoptera 2 Parasitic Hymenoptera 3 Parasitic Hymenoptera 4 Parasitic Hymenoptera 5 Parasitic Hymenoptera 6 Parasitic Hymenoptera 7 Parasitic Hymenoptera 8 Parasitic Hymenoptera 9 Parasitic Hymenoptera 10 Parasitic Hymenoptera 11 Parasitic Hymenoptera 12 Parasitic Hymenoptera 13 Parasitic Hymenoptera 14 Parasitic Hymenoptera 15 Parasitic Hymenoptera 16 Parasitic Hymenoptera 17 Parasitic Hymenoptera 18 Parasitic Hymenoptera 19 Parasitic Hymenoptera 20 Parasitic Hymenoptera 21 Parasitic Hymenoptera 22 Parasitic Hymenoptera 23 Parasitic Hymenoptera 24 Parasitic Hymenoptera 25 2 7 14 7 1 1 2 1 1 1 1 2 1 1 3 1 1 1 5 3 7 4 3 4 6 4 2 4 1 2 1 2 1 2 1 1 1 3 3 2 1 2 1 Parasitic Hymenoptera 26 Parasitic Hymenoptera 27 Parasitic Hymenoptera 28 Parasitic Hymenoptera 29 Parasitic Hymenoptera 30 Parasitic Hymenoptera 31 Parasitic Hymenoptera 32 Parasitic Hymenoptera 33 Parasitic Hymenoptera 34 Parasitic Hymenoptera 35 Parasitic Hymenoptera 36 Parasitic Hymenoptera 37 Parasitic Hymenoptera 38 Parasitic Hymenoptera 39 Parasitic Hymenoptera 40 Parasitic Hymenoptera 41 Parasitic Hymenoptera 42 Parasitic Hymenoptera 43 Parasitic Hymenoptera 44 Parasitic Hymenoptera 45 Parasitic Hymenoptera 46 Parasitic Hymenoptera 47 Parasitic Hymenoptera 48 Parasitic Hymenoptera 49 Parasitic Hymenoptera 50 Parasitic Hymenoptera 51 Parasitic Hymenoptera 52 Parasitic Hymenoptera 53 Parasitic Hymenoptera 54 Parasitic Hymenoptera 55 Parasitic Hymenoptera 56 Parasitic Hymenoptera 57 Parasitic Hymenoptera 58 Parasitic Hymenoptera 59 Parasitic Hymenoptera 60 Parasitic Hymenoptera 61 Parasitic Hymenoptera 62 Parasitic Hymenoptera 63 Pentatomidae 1 Pentatomidae 2 Pentatomidae 3 Pentatomidae 4 Anthicidae 1 1 2 1 1 2 4 5 6 2 4 10 6 1 1 2 1 5 5 1 3 1 1 1 5 1 1 5 8 1 1 1 1 1 1 1 1 1 1 3 9 3 1 95 Anthicidae 2 Anthicidae 3 Anthicidae 4 Coccinellidae 1 Coccinellidae 2 Coccinellidae 3 Coccinellidae 4 Coccinellidae 5 Coccinellidae 6 Coccinellidae 7 Coccinellidae 8 Coccinellidae 9 Coccinellidae 10 Coccinellidae 13 Coccinellidae 14 Coccinellidae larvae Thripidae 1 Thripidae 2 Thripidae 3 Thripidae 4 Thripidae 5 Thripidae 6 Thripidae 7 Hemiptera 1 Hemiptera Unknown 1 Hemiptera-Ant Mimic Hemiptera Unknown 2 Woodtick 1 Membracidae 1 Membracidae 2 Crustacea Armidillarium 1 Lygaeidae 1 Lygaeidae 2 Lygaeidae 3 Lygaeidae 4 Lygaeidae 5 Lygus 1 Elateridae 1 Scutelleridae 1 Centipede 1 Mirid 1 Mirid 2 Mirid 3 51 2 1 7 8 9 1 2 2 4 4 11 1 1 2 8 1 4 164 8 1 11 1 1 1 1 1 7 1 1 22 1 2 2 4 1 2 2 1 9 10 1 5 Mirid 4 Diabrotica virgifera (WCR) Diabrotica undecimpunctata Diabrotica barberi (NCR) Lacewing Larvae Green Lacewing 1 Brown Lacewing 1 Aphid 1 Aphid 2 Aphid 3 Aphid 4 Grasshopper 1 Grasshopper 2 Grasshopper 3 Grasshopper 4 Grasshopper 5 Grasshopper 6 Geocoridae Geocoris sp 1 Fulgoridae 1 Fulgoridae 2 Fulgoridae 3 Fulgoridae 4 Nitidulidae 1 Nitidulidae Glischrochilus 2 Hemiptera Psyllidae 1 Psyllidae 2 Psyllidae 3 Cercopidae 1 Tingidae 1 Tingidae 2 Millipede 1 Weevil 1 Weevil 2 Weevil 3 Weevil 4 Weevil 5 Chrysomelidae 1 Chrysomelidae 2 Chrysomelidae 3 Chrysomelidae 4 Chrysomelidae: Flea beetle 5 Delphacidae 1 Ephemeroptera 1 1 6 1 13 1 3 2 1 94 11 3 1 2 1 1 1 1 16 29 3 1 10 1 1 1 4 1 1 4 1 21 1 7 4 9 1 1 2 2 1 1 1 1 Phymatidae 1 Reduviidae 1 Mordellidae 1 Acanaloniidae 1 Scarab 1 1 3 1 2 9