Supplemental File 2: List of antibodies used in this study.
Name
Origin
-tubulin
DM1A, Santa
Cruz
ACTBD11B7,
Santa Cruz
custom
3-19, Abcam
-actin
CENP-A
CENP-A
CENP-A
CENP-B
CENP-C
HJURP
HJURP
TBP
H50, Santa
Cruz
ab25734
,Abcam
Ab33034,
Abcam
Bethyl
H277, Santa
Cruz
Ab63766,
Abcam
H5, Covance
Activated
RNA
Polymerase
II
(RNAPIIS2P)
RNA
CTD4H8,
Polymerase Santa Cruz
II
ChF: chromatin fiber
Immunoprecipitation
Dilution for
Western
Blot
Dilution for
immunofluorescence
1:100
1:100
1:1000
1:40000 (cell),
1:200 (ChF)
3 g
1:1000
3 g
1:1000
5 g
1:2000
1:1000
1:100
1:400 (cell), 1:50
(ChF)
1:400
1:2000
1:400 (cell), 1:50
(ChF)
1:500
1:500
1:50
1:1000