TEACHER PRE-LAB GUIDE
MATERIALS:
8 Groups (2 - 4 students per group)
7- 50ml plastic cups; 1 - 10ml vial of pre made nanogold colloid; 8 microcentrifuge test
tubes; 1 microcentrifuge test tube holder; 8 plastic pipets; 1 black Sharpie; 1 piece of
white paper; ¼ cup of colorless Propel (A), Pedialyte (B), Gatorade (C), Powerade (D),
Clausen pickle juice (E), pickling lime juice (F), distilled water (G). Bucket for liquid
waste. Student Lab Guide. Handout A. Handout B. Table I Data Collection. Pre and
Post Test.
DAY ONE:
Administer the Pre-Test; show 3-D video on nanotechnology; show a sample of
nanogold while asking Discussion Questions; go over Student Lab Guide in preparation
for the lab the following class day. Take up the Student Lab Guide.
DISCUSSION QUESTIONS:
a. What do you see athletes drink when they replenish their bodies loss of liquids?
Answers will vary.
b. What do you lose when you sweat?
Water and salts.
c. In your opinion what sports drink is the most beneficial?
Answers will vary.
d. What are the consequences of depleted electrolytes to your personal health and safety?
Muscle cramping, irregular heartbeat, heart attack, circulatory shut down, death, heat stroke,
dehydration.
e. Does anybody recognize the material in this vial?
Answers will vary.
f. Does anybody have any of this material in their possession?
Answers will vary.
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PREPARATION Prior to LAB:
For each Lab Station: 7 labeled plastic cups , A,B,C,D,E,F,G; pour ¼ cup of the
samples according to the materials list above; a test tube holder with 8 microcentrifuge
test tubes and 8 plastic pipets; a black Sharpie; a piece of white paper; a Student Lab
Guide; Handout A and Table 1 Data Collection.
DAY TWO:
Remind students of your Lab Safety Rules. Proceed with Lab.
Share information from groups on Handout A and Table 1 Data Collection; reveal to the
class the various solutions; show Power Point Presentation; give Handout B take home
activity; administer Post-Test; address any follow-up questions from the Lab activity.
ANSWERS TO HANDOUT A:
1. Answers will vary.
2. Nanogold is an indicator which changes color in the presence of electrolytes;
therefore, it is the standard for comparison in the experiment.
3. The variables are the samples of liquids being tested.
TABLE 1: DATA COLLECTION:
Answers will vary according to group findings; however, if conducted accurately, there
should be some consistency in the results. In general, an increase in nanogold size
would indicate more electrolytes. The color change would go from the original pink
color to blue green.
HANDOUT B:
Answers:
4. 1,000,000; 5. 100,000; 6. 10,000; 7. 1000; 8. 100; 9. 10
Prepared by: Dr. Debbie Payne, Ruth H. Liddell, Shirley K. Scarbrough
Alabama State University, Math, Science Partnership, Spring 2013
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