Supplementary Table 1. SNPs associated with more than one rubella-specific immune response outcome after MMR vaccination in the
Caucasian Cohort.
Cohort
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
Study
Rubella
Vaccine
SNP ID
rs157582
++
Chromosome
/ Position
19 / 4.5x107
Gene
Location
TOMM40
Intron
rs1725671
3
+NA
rs2229857
+IFN-γ
14 / 2.5x107
IRF9/
REC8
Intergenic
1 / 1.5x108
ADAR
Coding
rs2291842
++
11 / 5.7x106
TRIM22
Coding
rs2393657
++
6 / 2.6x107
BTN2A1/
BTN3A3
Intergenic
rs3440455
4
++
rs474247
+IFN-γ
19 / 4.5x107
TOMM40
Intron
1 / 1.2x107
TNFRSF1B
Intron
rs6090086
6
++
rs6857
+IFN-γ
6 / 2.6x107
BTN2A1/
BTN3A3
Intergenic
19 / 4.5x107
PVRL2
UTR
rs6900118
++
6 / 2.6x107
BTN2A1
Intron
rs7135223
8
++
rs8104483
++
19 / 4.5x107
PVRL2/
TOMM40
Intergenic
19 / 4.5x107
PVRL2
Intron
Immune
Measure
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
(ng/μl)
Secreted
IL-6
N
Genotype
No.
826
GG
GA
AA
GG
GA
AA
GG
GA
AA
AA
AG
GG
CC
CA
AA
GG
GC
CC
GG
GA
AA
AA
AG
GG
GG
GA
AA
GG
GA
AA
AA
AG
GG
AA
AC
503
283
40
682
139
8
442
308
78
571
229
29
752
71
0
612
198
18
539
258
31
814
13
0
571
226
21
739
85
2
608
202
19
465
311
829
828
829
823
828
828
827
818
826
829
829
Immune Response
Outcome (IQR)
3593.7 (3046.8, 3967.6)
3666.1 (3185.8, 4052.0)
3842.6 (3061.2, 4120.8)
3650.5 (3115.1, 4024.2)
3489.9 (2996.6, 3969.6)
3571.4 (3173.6, 3816.7)
3617.4 (3081.3, 4003.9)
3634.2 (3120.0, 4027.3)
3743.5 (3099.0, 3975.2)
3632.1 (3102.4, 3988.8)
3654.0 (3081.3, 4052.0)
3305.5 (3058.5, 3780.5)
3637.4 (3092.2, 4016.6)
3498.4 (3126.7, 3832.4)
P Value
3621.1 (3049.7, 3996.5)
3660.2 (3185.8, 4033.6)
3667.3 (3023.6, 3968.5)
3614.8 (3098.7, 4041.3)
3637.4 (3119.7, 3968.5)
3694.1 (3024.8, 3985.1)
3633.0 (3098.7, 4003.7)
3614.8 (3008.9, 4028.7)
0.0276
3609.8 (3043.4, 3976.4)
3685.7 (3207.9, 4080.6)
3553.4 (2913.5, 3868.6)
3644.4 (3098.7, 4024.9)
3525.5 (3064.6, 3832.4)
3713.8 (3398.9, 4028.7)
3617.3 (3049.5, 3993.1)
3668.1 (3185.8, 4033.6)
3837.8 (3023.6, 4095.1)
3654.4 (3130.2, 4033.6)
3596.1 (3066.3, 3988.6)
0.0268
0.0075
0.0003
0.0208
0.0446
4.2x10-6
0.0437
0.0050
0.0273
0.0208
0.0397
Immune
Measure
Secreted
IFN-γ
(ng/μl)
NA (NT50)
N
Genotype
No.
826
GG
GA
AA
GG
GA
AA
GG
GA
AA
AA
AG
GG
CC
CA
AA
GG
GC
CC
GG
GA
AA
AA
AG
GG
GG
GA
AA
GG
GA
AA
AA
AG
GG
AA
AC
503
283
40
723
148
8
442
308
78
571
229
29
752
71
0
612
198
18
539
258
31
865
12
0
571
226
21
739
85
2
608
202
19
465
311
879
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
NA
(NT50)
828
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
(ng/μl)
Secreted
IFN-γ
818
829
823
828
828
877
826
829
829
Immune Response
Outcome (IQR)
5.9 (1.6, 19.2)
7.0 (1.6, 20.8)
8.7 (2.1, 24.3)
56.5 (35.1, 92.6)
50.2 (31.4, 88.5)
36.6 (30.2, 67.0)
6.0 (1.5, 19.9)
6.3 (1.8, 18.0)
9.0 (2.7, 22.1)
6.2 (1.8, 19.9)
6.1 (1.4, 17.3)
12.7 (2.7, 38.3)
6.4 (1.7, 21.5)
6.1 (0.8, 13.7)
P Value
6.3 (1.7, 19.0)
6.0 (1.2, 20.8)
15.6 (4.0, 40.4)
6.7 (1.6, 21.7)
6.3 (2.0, 18.0)
3.9 (1.0, 9.4)
55.4 (34.4, 89.9)
94.0 (60.3, 138.4)
0.0144
6.3 (1.7, 19.2)
6.0 (1.4, 18.8)
10.8 (2.8, 30.7)
6.2 (1.7, 20.8)
6.5 (0.9, 13.8)
9.6 (4.1, 15.2)
6.2 (1.7, 19.2)
6.2 (1.3, 19.9)
14.5 (4.0, 40.4)
7.0 (2.1, 21.9)
5.1 (1.3, 18.0)
0.0244
0.0063
0.0357
0.0447
0.0328
0.0082
0.0265
0.0053
0.0128
0.0092
0.0172
Study
Rubella
Vaccine
Study
rs962859
+IFN-γ
21 / 3.5x107
IL10RB
Intron
(ng/μl)
Secreted
IL-6
(ng/μl)
816
CC
AA
AC
CC
53
257
424
135
3385.3 (2866.8, 3877.8)
3682.6 (3183.3, 4052.0)
3610.5 (3048.0, 4001.7)
3540.2 (3090.5, 3888.5)
0.0023
(ng/μl)
Secreted
IFN-γ
(ng/μl)
816
CC
AA
AC
CC
53
257
424
135
5.9 (1.1, 12.4)
7.2 (2.1, 23.4)
5.0 (1.4, 17.4)
7.6 (2.3, 21.3)
0.0329
Abbreviations: NA-neutralizing antibody; SNP ID-single nucleotide polymorphism identification; IL-interleukin; IFN-interferon; IQR-interquartile range; NT50-neutralizing titer; A-Adenine; CCytosine; G-Guanine; No.-number
+One phenotype failed to remain significantly associated with genotype as determined by having a q-value greater than 0.1.
++Two phenotypes failed to remain significantly associated with genotype as determined by having a q-value greater than 0.1.