SUPPLEMENTAL TABLE 2
Primers used in this study
Primer
OD16
OD21
O276
O277
O278
O279
O280
O245
O246
OD18
OD21
OD51
O780
OD25
OD30
OD52
O780
O274
O290
O294
O295
O588
O589
O272
O291
O304
O343
O580
Sequence
Purpose
Primers to delete genes and check for deletion
tgactgggattccgtgagacggac
Delete C. glabrata PHO4 with
KANMX6
atcttgtgccctccaaacagaactg
aagacccacaaaagaagaataagccaaaacaaaggaaataATGTCT
AAAGGTGAAGAATT
gtaggccctatctcttcttcaTCGATGAATTCGAGCTCGTT
AACGAGCTCGAATTCATCGAtgaagaagagatagggcc
tac
gtcgtgtcgtgatagaatactg
gctaagactacaatgtgcgg
GGGACAACACCAGTGAATAATTCTTCACC
ATGGAACTGGCAATTTACCA
ctgttagtgatgggtgaacaggtcggatccccgggttaattaa
atcttgtgccctccaaacagaactg
TACGAGTGTAATAATCGTTGG
TTAATTAACCCGGGGATCCG
gctcgctctccattgcctattgtag
tcccatccaatcttatccggtgc
TGTCCCGGTACAGCTGCTGC
TTAATTAACCCGGGGATCCG
ataaaaaccggcacgcaatagtacttattttctgttagtgcacaggaaacagc
tatgacc
agtagtcatttgaagttttgtcttggagtatctgtagtcaGTTGTAAAA
CGACGGCCAGT
ataaaaaccggcacgcaatagtacttattttctgttagtgatgggccgtacaa
cttctga
agtagtcatttgaagttttgtcttggagtatctgtagtcacgtgctcacgttctg
ctgta
cgatgaccaccacactgttgaggactgtgggattgcgctgcggatccccg
ggttaattaa
cgttggtgccccgcagacagtccacatgcatggtgatacgGAATTCG
AGCTCGTTTAAAC
gagatgagcaaaggagacagaacaagagtagcagaaagtccacaggaa
acagctatgacc
ccgatatgcccggaacgtgcttcccattggtgcacggtcaGTTGTAA
AACGACGGCCAGT
gagatgagcaaaggagacagaacaagagtagcagaa
agtcATGggtgaacaggtagagga
ccgatatgcccggaacgtgcttcccattggtgcacggtcatttcttgtactg
ctcaagct
tgtccagtgcctcatatttacaaagagccgaggctcaccgctctagaact
agtggatcc
Fusion primers: Delete CgPHO84
with XFP-KANMX6
Fusion primers: Homology to XFP 3'
end to delete CgPHO84 with XFPKANMX6
Check primer for CgPHO84 deletion 5'
Check primer for XFP - 3'
Check primer for XFP - 3'
Delete CgPHO4 with NATMX6
Check primer for CgPHO4 deletion
PringleChk
Delete CgPHO2 with NATMX6
Check primer for CgPHO2 deletion
PringleChk
Precisely delete CgPHO4 with
CgURA3
Replace CgURA3 with ScPHO4 in
DG37
Delete CgHIS3 with NATMX6 in
DG38
Precisely delete ScPHO4 with
ScURA3
Replace ScURA3 with CgPHO4 in
DC33
Amplify C. glabrata promoter-YFP
constructs to integrate into the URA3
O789
O375
ccataatataaattatcagatcg
taggttatggtttaatgtg
O815
gcaaactagcgcaataaaggatg
O662
atgtttaaatctgttgtttattcaattttagccgcttcttCGGATCCCCG
GGTTAATTAA
ttgtctcaatagactggcgttgtaatgagtagtgttccagGAATTCGA
GCTCGTTTAAAC
cgaagagcttgagcagtacaagaaacggatccccgggttaattaa
Fusion primers: Amplify YFPKANMX6 to tag CgPHO4
gttttgtcttggagtatctgtagaattcgagctcgtttaaac
agacgaggcttccaacgatgcaag
Fusion primers: Homology to 3' end
of CgPHO4
ttaattaacccggggatccgtttcttgtactgctcaagctcttcg
TCT AAA AGA GTT TTC AAG GCG CCA GCG
Check primer for integration of YFP
GAT ATT TCA AGC
downstream of CgPHO4
GGGACAACACCAGTGAATAATTCTTCACC
Check primer for XFP - 3'
Primers to make plasmids
CGATCGGCGGCCGCGTATGCATTACCTTCTCA Amplify C. glabrata PHO4nophos with
ATTAGG
5' NotI and 3' PacI restriction sites for
CGACACTTAATTAATTTCTTGTACTGCTCAAG
cloning into pRS313-myc13
CTCTTCG
CAAATAAATATCCACTCTCGTTACAATGatccttc 1 kb CgPMU2p-PMU2 in pRS313ataaattgctattgttg
myc13
accagtgaataattcttcacctttagacatTTAggatactacgatgaccg CgPMU2 stop codon 3'
at
gtggcggccgctctagaactagtggatcccgcgaagtcgagatgacagtg 3.6 kb (genomic clone) CgPMU2pPMU2 in pRS313-myc13
ccagtgaataattcttcacctttagacatgcaatacaccagttctgaagg
CGATCGGCGGCCGCgttccttggttatcccatcg
ScPHO5p-PHO5 in pRS313-myc13
O663
OD24
OD20
OD22
OD23
O98
O245
DWO5
DWO6
O213
O483
O584
O585
O480
O481
O308
locus of a C. glabrata wild-type strain
Check primer for promoter-YFP
integration into URA3 locus - 5'
Check primer for promoter-YFP
integration into URA3 locus - 3'
Delete ScPHO5 with KANMX6 in
DC41
O309
CGACACTTAATTAAttgtctcaatagactggcgttg
ggtggcggccgctctagaactagtggatccaataacaaaatgtctaaagg vYFP-KANMX6 in polylinker of
gatatcgaattcctgcagcccggggctacccgaggcaagctaaacagat pRS313-myc13
O787
c
gatctgtttagcttgcctcgcaagacatatccaataggaagc
O788
O318A
ggtatcgataagcttgatatcgaattcctgcagcccataatataaattatca
gatcg
ggtggcggccgctctagaactagtggatcccacttctattactcctatcac
ttg
O319
ggtggcggccgctctagaactagtggatcccgaagtcgagatgacagtg
c
O320
ggtggcggccgctctagaactagtggatcctgataccttatgtcgctaat
O430
ggtggcggccgctctagaactagtggatccATTTATCAACGAA
TAACCAG
Insert CgURA3 homology sequence
downstream of vYFP-KANMX6 in
polylinker of pRS313-myc13
Amplify 3 kB CgPMU2p - 5' (for gap
repair into vYFP-KANMX6-CgURA3pRS313-myc13)
Amplify 2 kB CgPMU2p - 5' (for gap
repair into vYFP-KANMX6-CgURA3pRS313-myc13)
Amplify 1 kB CgPMU2p - 5' (for gap
repair into vYFP-KANMX6-CgURA3pRS313-myc13)
Amplify 900 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
O431
ggtggcggccgctctagaactagtggatccGAGAAACACTAA
GCATCTAAGC
O432
ggtggcggccgctctagaactagtggatccATTAAATTAATTG
TAAATTAAC
O433
ggtggcggccgctctagaactagtggatccATGCACTCTTTTT
GAATATG
O434
ggtggcggccgctctagaactagtggatccGGATAACTTGATT
TTTCTTGC
O435
ggtggcggccgctctagaactagtggatccGAATTTTAACAGT
ACAGC
O949
ggtggcggccgctctagaactagtggatccgctcagtatctttactttttaa
tta
O950
ggtggcggccgctctagaactagtggatccaattaaatatctcatttctcc
g
O951
ggtggcggccgctctagaactagtggatcccgccccgtgtatgcctcctc
c
O952
ggtggcggccgctctagaactagtggatcccatgtattttaaataatcaa
at
O1087
ggtggcggccgctctagaactagtggattatgcctcctccatgtattt
O321
accagtgaataattcttcacctttagacattgtaacgagagtggatattt
O320
ggtggcggccgctctagaactagtggatcctgataccttatgtcgctaat
O1089
O1088
O321
O322
tttaaaatacatggaggaggcataggggcggagaaatgagat
atctcatttctccgcccctatgcctcctccatgtattttaaa
accagtgaataattcttcacctttagacattgtaacgagagtggatattt
ggtggcggccgctctagaactagtggatcctcagcgtcatgttccccaga
O429
ggtggcggccgctctagaactagtggatccGTGATTGACACA
CAGAACACAG
O883
ggtggcggccgctctagaactagtggatcctggtgcgctaagactactgc
O323
accagtgaataattcttcacctttagacattatttcctttgttttggctt
Amplify 800 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 700 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 600 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 500 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 400 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 780 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 760 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 740 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 720 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 730 bp CgPMU2p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify at start codon CgPMU2p - 3'
(for gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Fusion PCR primers to mutate
CCCGTG (to delete GTG) in 1 kb
CgPMU2p-YFP
Amplify 1 kB CgPHO84p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 400 bp CgPHO84p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify 300 bp CgPHO84p - 5' (for
gap repair into vYFP-KANMX6CgURA3-pRS313-myc13)
Amplify at start codon CgPHO84p 3' (for gap repair into vYFPKANMX6-CgURA3-pRS313-myc13)
O429
O995
O994
O323
O75
O76
O78
O79a
O664
O641
O155
O156
O153
O154
O151
O152
O1207
O1208
O1191
O1192
O1193
O1194
O1201a
O1202a
O1203
O1204
O1205
O1206
O1211
O1212
O1225
O1226
O1221
O1222
O1217
O1218
O1229
O1230
ggtggcggccgctctagaactagtggatccGTGATTGACACA
CAGAACACAG
gtattagattaggaccaGaGCtCcatgtgggcccctctttc
gaaagaggggcccacatgGaGCtCtggtcctaatctaatac
accagtgaataattcttcacctttagacattatttcctttgttttggctt
qPCR primers
gaccaaactacttacaactcc
ccactttcgtcgtattcttgcttg
gccttgggtactctgatcgacc
gtgctgtcggcgacagtaac
gtgatgttactgttcattttgattatcctg
gatacatcgatcacagtgtaaactag
TCCTACGAACTTCCAGATGGT
GGCAGATTCCAAACCCAAAA
GCCGGTTCCAAAAACGTTTA
GACAGTGAAGACGGATACCCA
TGCTTGTAACTCATGTCCTGC
TTGAGGTCAAGTTCAAACCCT
ChIP primers
Fusion PCR primers to mutate
CACGTG (ScPho4 binding site) in
400 bp CgPHO84p-YFP
acggcccatcagcagcagcttc
cttcatagcacagagctcatagc
tgaagacactgagagcacgcg
atcacatttccactgcggggc
aatccatataagtgggaagcg
aggtccccgcagaattctccg
ctgctatactgctaagactaag
tagattaggaccacacgtgc
tgtgcggaaagaggggcccac
caaattttttttccctgcatc
agctcatctctaatatttccaat
agcaacttggatccggtagtt
gcgtgcggtaaagtcggtgcc
aaccaacagttcttaggcttgc
gcatagctatttcaatgtcttc
ttcttaaagaactagggaggcac
acatcatactaagatcaaaaaag
aggaggcatacacggggcggag
tctcacgtatagctgagattg
acattattgtttatgcatgtc
-950 bp CgPHO84 promoter
AGATAAGTTTTTCGTTTCACCAT
ATGAACAGTGGAATTTGCTGGC
CgACT1 5' qPCR
CgACT1 3' qPCR
CgPHO84 5' qPCR
CgPHO84 3' qPCR
CgPMU2 5' qPCR
CgPMU2 3' qPCR
ScACT1 5' qPCR
ScACT1 5' qPCR
ScPHO84 5' qPCR
ScPHO84 3' qPCR
ScPHO5 5' qPCR
ScPHO5 3' qPCR
-450 bp CgPHO84 promoter
-400 bp CgPHO84 promoter
-200 bp CgPHO84 promoter
-150 bp CgPHO84 promoter
-100 bp CgPHO84 promoter
+1500 bp CgPHO84 promoter
-2333 bp CgPMU2 promoter
(CACGTG)
-800 bp CgPMU2 promoter
-500 bp CgPMU2 promoter
+500 bp CgPMU2