Table S1. BAC-probes used for der(2) and der(8) breakpoints characterization in FISH assays.
BAC-PROBES
2p16.1
8q23.3
RP11-260K8
CTD-2314I21
RP11-473J21
RP11-91D24
CTD-2310O20
RP11-1070E3
RP11-158B2
RP11-831B1
RP11-143P23
CTD-2165G15
CTD-3240M24
CTD-2173M9
RP11-133H5
RP11-185B15
RP11-66L24
CTD-2562H20
RP11-1079H24
RP11-137E3
CTD-2176M10
Table S2. Primers used to create Long Range PCR probes for FISH assays on chromosome 2
(chr2:59549048-59593858, hg 19).
LR
Primer Fw
TCCAATCAAGCATACACAAAACACTTCT
Tm = 56.9°C
PROBE 1
Primer Rev
ACAATCATTTTACTTGCTTCCTCTCAGC
Tm = 58.4°C
Amplicon size
14637 bp
TA = 60.7°C
LR
Primer Fw
TCAGAAACCTATGGAATAGAGGATTGGA
Tm = 58.4°C
PROBE 2
Primer Rev
TTTCTCAATGAAGTGTGCATTAGTTGGT
Tm = 56.9°C
Amplicon size
15487 bp
TA = 60.7°C
LR
Primer Fw
ATTTGTCAGGACCACATTTTTCCATTAC
Tm = 56.9°C
PROBE 3
Primer Rev
ATACCACAGTTGCCTTCCTAAGAAACCT
Tm = 59.9°C
Amplicon size
15079 bp
TA = 61.4°C
Table S3. Primers used for der(2) and der(8) breakpoints molecular characterization.
BKP der(2)
Primer Fw
GTTGACATCAGGACTTCAGGTAAATGAA
Tm = 58.4°C
Primer Rev
AATTTCTCCTTTATTCCTCTCCCCTTTC
Tm = 58.4°C
TA = 61.4ºC
BKP der(8)
Primer Fw
CCTTCTAGAGCAAATTCTTTTAGACCTTGA Tm = 58.8ºC
Primer Rev
CTCATGGTGTAGAATAGAAGCAGCAAGT
Tm = 59.9ºC
TA = 62.4ºC