Table S1. Precursor and product ion combinations (quantifier underlined) used to monitor
lidocaine and its metabolites by LC-MS/MS in positive mode, including their respective mass
spectrometry parameters.
Component
DMA
4-OH-DMA
Precursor ion (m/z)
Product ion (m/z)
DPa
CEb
CXPc
122.1
105.0
31
23
8
77.0
31
37
12
121.1
81
21
8
77.0
81
33
12
138.0
DMA-D6
128.0
111.1
56
23
8
MEGX
207.3
58.1
36
17
8
77.2
36
75
4
MEGX-D5
212.2
63.1
31
19
8
LIDO
235.1
86.1
61
23
8
58.0
61
49
10
LIDO-D10
245.2
96.2
66
25
8
3-OH-LIDO
251.1
86.1
56
23
8
58.0
56
53
10
86.1
61
23
8
58.1
61
51
8
91.1
60
33
13
4-OH-LIDO
3-OH-LIDO-D5
a
251.3
256.2
: DP: declustering potential (in volts), b: CE: collision energy (in volts), c: CXP: collision exit
potential (in volts)
Table S2. Milk production by the cows during different intervals, expressed in kg
Time
3-h Exp.
48-h Exp.
after
injection
(h)
Cow 1
Cow 2
Cow 3
Cow 4
Cow 5
Cow 6
Cow 7
Cow 8
-0.2
1.0
0.2
0.4
0.8
0.26
0.83
0.94
0.10
0.5
0.4
0.1
0.1
0.8
0.13
0.35
0.23
0.45
1
0.3
0.1
0.2
0.5
0.07
0.07
0.12
0.15
2
3.9
0.2
0.1
0.3
0.77
0.17
0.14
0.16
3
0.6
0.1
0.3
1.1
-
-
-
-
4
1.83
5.93
0.74
8.74
8
13.27
3.52
8.43
5.74
12
10.55
0.75
5.06
1.50
24
23.90
16.60
13.02
16.20
36
6.23
10.64
12.82
11.73
48
21.55
15.10
13.37
15.22
Table S3. Urine production by the cows during different intervals, expressed in kg
Time
48-h Exp.
after
injection
(h)
Cow 5
Cow 6
Cow 7
Cow 8
-0.25
0.58
1.82
0.51
6.91
3
0.95
5.12
4.00
5.45
6
2.78
6.92
2.83
3.50
9
3.14
5.22
2.88
2.63
12
2.62
4.98
3.39
4.57
24
7.83
17.39
12.89
13.18
36
10.26
14.33
7.39
11.00
48
14.50
29.29
7.00
10.41
Table S4. Tissue and organ weights at slaughter
3-h Experiment
Tissue/organ weight
(kg)
Cow 1
Cow 2
Cow 3
Cow 4
0.71
0.82
0.80
0.92
13.49
11.34
10.30
12.90
Kidneya
1.62
1.89
1.84
2.22
Longissimus dorsia
4.22
3.44
2.94
5.54
Perinephric fat
4.71
0.58
0.33
1.18
Injection site paralumbar fossa
Liver
48-h Experiment
Cow 5
Cow 6
Cow 7
Cow 8
1.95
1.36
0.72
1.09
14.02
12.62
10.05
11.29
Kidneya
2.34
2.35
2.12
1.78
Longissimus dorsia
4.53
3.44
3.86
4.97
Perinephric fat
1.43
0.60
0.98
2.78
Injection site paralumbar fossa
Liver
a
Only one kidney or muscle from one side
Figure S1. Concentrations of lidocaine and metabolites in milk samples of individual cows
injected with lidocaine and killed after 48 h.