tpj12452-sup-0013-TableS2

advertisement
Table S2. Oligonucleotide primer used in this study.
Name
Purpose
Sequence
RbohH spec For
GAGCCTACTCAAGCAGCCG
RbohH EcoRI for
genotyping of SALK
058170 and
SALK_136917
genotyping of SALK
058170 and
SALK_136917
genotyping of
SAIL_31_D07
genotyping of
SAIL_31_D07
Genotyping rbohJ1(GK_787_E04)
Genotyping rbohJ1(GK_787_E04)
Left border primer GK
lines
Left border primer
SALK lines
Left border primer
SAIL lines
cloning of RbohH CDS
RbohH FL SmaI rev
cloning of RbohH CDS
AACCCGGGTTAAAAATTTTCCTTGTGG
RbohJ EcoRI for
cloning of RbohJ CDS
CCGAATTCATGAAAAACAACAAAAAGGTTGG
RbohJ SmaI rev
cloning of RbohJ CDS
ATCCCGGGTCAGAAATTCTCTTTGTGAAAAG
ProRbohH_675bp
AscI for
cloning of upstream
intergenic region of
RbohH
cloning of ProRbohH
into pXNS2 YFPRbohH
RT-PCR detection of
RBOHH
RT-PCR detection of
RBOHH
RT-PCR detection of
RBOHJ
RT-PCR detection of
RBOHJ
RT-PCR primer
Ubiquitin associated
for control
RT-PCR primer
Ubiquitin associated
for control
TTGGCGCGCCGATTTTGATTTTTG
RbohH geno rev
SALK_058170/136917
RbohJ geno for
SAIL_31_D07
RbohJ geno for
SAIL_31_D07
rbohJGK_787_E04_For
rbohJGK_787_E04_Rev
GABI LB primer o8409
SalkLba1
LB1 for Sail lines
ProRbohH SacI rev
RbohH spec For
RbohH geno rev
SALK_058170/136917
RbohJ geno for
SAIL_31_D07
RbohJ spec Rev
Ubiquitin assoc. for
Ubiquitin assoc. Rev
CTTCGTACATGCTTGTTAAGTAGTTATGC
GAATCTCTTGGGAGCCAAGC
CTCCTAATGCTCTTATGTGAACGC
AAGGCGATAGTTCTGTGAAACAACC
TACCCAATGCTTCGTATACTTTTGC
ATATTGACCATCATACTCATTGC
TGGTTCACGTAGTGGGCCATC
TTTTCAGAAATGGATAAATAGCCTTGCTTC
CCGAATTCATGAAAAGTAACACTCCTACGG
AAGAGCTCAGTATTATTATTTATTCTTTCTCCGCC
GAGCCTACTCAAGCAGCCG
CTTCGTACATGCTTGTTAAGTAGTTATGC
GAATCTCTTGGGAGCCAAGC
TCCATATACACTTGATCCTCCTAAGC
AAGATGAAATCGAGAGACAAGAAGGTC
GCTACTTTTGCCCCACCATCATC
Download