Supplementary Table S3. Free Energy Flux of Calculated Redox Reactions
ΔG
(kJ/mol†)
Free Energy Flux
(kJ cell-1 sec-1)
2H2 + O2  2H2O
-216
-1.3x10-13
0.4Propane + SO42-  HS- + 1.2HCO3- + 0.2H+ + 0.4H2O
-21
-1.2x10-13
CO + 2H2O  H2 + HCO3- + H+
-25
-1.1x10-13
0.3077n-Butane + SO42-  HS- + 1.231HCO3- + 0.2308H+ + 0.3077H2O
-22
-4.6x10-14
H2 + 0.4H+ + 0.4NO3-  0.2N2 + 1.2H2O
-191
-3.4x10-14
Methane + 2O2  HCO3- + H+ + H2O
-204
-3.1x10-14
Formate + 0.5O2  HCO3-
-215
-3.0x10-14
NH3 + 1.5O2  NO2- + H+ + H2O
-91
-2.0x10-14
Ethane + 3.5O2  2HCO3- + 2H+ + H2O
-208
-1.8x10-14
Propane + 5O2  3HCO3- + 3H+ + H2O
-187
-1.1x10-14
3H2 + CO  Methane + H2O
-33
-9.8x10-15
n-Butane + 6.5O2  4HCO3- + 4H+ + H2O
-144
-7.3x10-15
4H2 + H+ + SO42-  HS- + 4H2O
-28
-6.2x10-15
n-Butane + 4H2O  2Acetatic acid + 5H2
-13
-4.4x10-15
Acetate + 2O2  2HCO3- + H+
-206
-3.9x10-15
4H2 + H+ + HCO3-  Methane + 3H2O
-12
-2.7x10-15
4H2 + 2HCO3- + H+  Acetate + 4H2O
-10
-2.2x10-15
Acetate + 1.6NO3- + 0.6H+  2HCO3- + 0.8H2O + 0.8N2
-181
-2.1x10-15
4Formate + H+ + SO42-  HS- + 4HCO3-
-28
-9.5x10-16
HS- + 2O2  SO42- + H+
-188
-4.2x10-16
4Formate + H2O + H+ Methane + 3HCO3-
-47
-4.1x10-16
Acetate + SO42-  2HCO3- + HS-
-18
-3.4x10-16
H2 + Pyrolusite + 2H+  Mn2+ + 2H2O
-165
-2.8x10-16
HS- + 4NO3-  SO4-- + 4NO2- + H+
-118
-2.6x10-16
H2 + 2Fe(OH)3 + 4H+  2Fe2+ + 6H2O
-72
-1.2x10-16
Acetate + H2O  Methane + HCO3-
-8.7
-4.2x10-17
5HS- + 8NO3- + 3H+  5SO42- + 4N2 + 4H2O
-93
-4.1x10-17
Acetate + 3H2 + H+  Ethane + 2H2O
-5.1
-2.4x10-17
Acetate + HCO3- + 6H2 + 2H+  Propane + 5H2O
-4.3
-2.1x10-17
Acetate + 4Pyrolusite + 7H+ -> 4Mn2+ + 4H2O + 2HCO3-
-155
-1.4x10-18
Acetate + 8Fe(OH)3 + 15H+ -> 8Fe2+ + 20H2O + 2HCO3-62
-5.7x10-19
†kJ/mole of 2e-. * The hydrolysis of butane represents an abiogenic reaction that is kinetically inhibited, but no known
microorganisms has been isolated that performs this function.
A complete table of calculated redox reactions is shown. This is an extension of Table 3 in the main text where G is in situ
Gibbs free energy of the limiting reactant (kJ/mol) and free energy flux (kJ cell-1 sec-1).