Supplementary figure
Genomic DNA sequence of cold-adapted PI12 serine protease (4081 bp) (5’-3’)
Partial sequence from recombinant
plasmid (shotgun cloning) (1321 bp)
Full genomic DNA sequence of cold-adapted PI12 serine protease (4081 bp)
Partial sequence from DNA Walking
approach (1531 bp)
TCACAGAAGTATGCCAAGCGAGGGGGGGGGGTCTGCTAGCGCGAATCGAGAAAACACAAAAGAACCTCCC
GACTTGGCGTTGCTTCTGCTCTTCTCCTTCTCCCAACCGCCGACAATAAAAACTATAACACACACACACC
CCATCTCGACGCCTGCAGACTTCACGACTCTCCTCACGCTCTTTACCGCCCCACGGGTCCCACCCACGAG
CCACGATGCTCTTCCTCCCCGTCCTCCTCCTCCTCCTTCCCGGCGTCACTGCCTTCCTCAACCCAGTTAC
CAACCGCGCGACCAACGCCATCTCCTCGACTCAATACCTCTCTAACGCCTATATCCTCGAATTGGACCTC
TCCACCCCCGGCCTCGTCAAACGGGATAGCACGCCCGACTCTGTGCGTCCCGCGTTCCTCCCTCGCTGTC
GAAGACGAGCTAACAATGACGTGGTAGATCCTAGAGGACGTACTCACGTCCGTAGGCCGCAACGGTATCA
AGTACCAACTCCGCCACCGCTTTATCTCCCCGACTCTGTTCCACGGCGCTTCGATCACTGTCCCCCCTGG
AATCTCCCGCTCCCAAATCGCCTCTCTCCGCGGTATCAAAGTCCGTCCCTCTCTCTCCCCTACTCCCTCC
GTGCTAACCCTGCACGCAACAGCGCGTCTGGCCCGTTCGAAAGTTCTCCCGACCCAGCGCAGTAGTGGAC
GCCGATGGAGGAGGAAGCGGGTTCTCAGGGTCGCCTATCAAGGCGGCGCTCATGGGGGTGAAAGAGCTCG
GGAAGCGCGCGAACGCTTATGCTGGAGATACGTTTGGACCGCATGTCATGACGGGGGTTAATGAGACGCA
TGAGGCGGGGTTGTTGGGAGCTGGGATTAAGGTGTGTTTTCGTCTTTGTTTGGGGGAGGGGGGAGGGAGG
AGCTGATTAATGGGTGCAGATTGGGGTGCTGGACACTGGTGAGAAGCGGTTTGGAGGGAGGAGAGGATGG
AGCTGATGAGTGTGCAGGTGTTGATTATTTGAACCCGATTCTGGGAGGCTGCTTTGGACCTGGGTGCCAT
ATGTCGTTTGGGTACGACTTGGTTGGCGATGATTACGATGGAGATAACGCTCCTGTGCCGGATGTGGATC
CTTGTGCGTCCTTCCCTCCGCGATGGGCTGGAATCTGAGCTGACGGCGTTGTTGATCAGTTGCGAGCTGC
GATCCTCATGGTTCGTCTCGCTTTCCGCTCCTCTGATGCGCTCGCTGATGCACTTGGCAATAGGAACTCA
CGTTACGGGAATCATTGGAGCGCTCCCGAATGCGTTTGGATTTACTGGCGTCGCACCCGCCGCTACTCTG
GGCCACTACCGAGTGCGCTCCCTCTATCCGTTCTTCCTCGCAGCTCCCACTCACTCGTGCTTCCAGGTAT
TTGGCTGCACTGGCTTCGTCGGAGAAGATATCGTTCGTCCTCTCTCCAGTCCCGGCTAGCGATCCTGCTT
CTGACTTCGCATCTCCCTCTGAGATTCTCGCTGGACTCATGCGAGGAGTCGAGGACAACTGCAACGTCTT
GACCCTCTCTCTCGGAGGTCCAGGAGGGTGGGTCAAGGGCACGCCGGCGTCCATCCTTATCGACCAGATC
GAAGCGCAAGGCATTCTCGTCACCGTCGCCACTGGCAACTCGGGAGCTGAGGGTGAGCTCCTCGCTACTT
GCGCGTCGAGACCAGTACTAACAGGGGCGTGGACGCAGGCATGTTCTTCTCCGAGTCTCCCGCCTCGACG
ATCAACGGCCTTTCCATCGCATCCACGTCCGTCTCTCCCTTCCTTCGCACGCCACTCGCTCACACTCTCT
CGCAGGGACGTTACCGACCTCATCGCCTACAACGCCACCGTCTCAGGCCAACCTGCGATCCCTTACCTCT
CCGCCACGCCCCTCAACGTCGTCGCCAACAGCTTCCGCGTCCACTTCACCTCTACCGACCCCAACAACCC
CGTCGACGCCTGCTCTCCTCTTCCGGCTGGAGCGCCCGACTTCGCCAACTATGTTACGGTCGTTCAGCGT
GGGACTTGTACGTTCGTTACCAAGTACCAGAACGTTCTCAATGCTGGAGGGTGCGTCGGCTTCTTCTCTC
CCGACTGGATTGGGCGGAGACTGACCTTCTGAGCAGAAAGATCGTATTGTTGTACAACTCGGAGGGAGCT
GGGAACCTCCCTTACCTCACGCCCAACGGTGTCGGCATCGACGCCGTTGCAGGTCTTCGTCGTTCCGACG
GACTCAAGGTTCGTCTACGCTCTTCGGTGCTGAGGCCCTTCTCTGATCTCGCTCCGACAGCTTCTCTCGT
ACTATCAGAATGCCAACAAGCGTCTCACTCTGCGCTTCCCCAAGGGCAAGATCGTCGCAGGCTTGACCGA
TACCATCACCGGCGGACTCATCTCGTTCGTTCCTCTCCGCCTCCCTCGCTGGCCGATGAGCTGACGCGGT
CTTTGCAGGGGTTACTCGACGTTTGGTCCGACGAATGACCTCTACGGTCAGCCTACCCTCTCTGCCCCTG
GTGGCAACATCCTTTCGACCTTCCCTCTCTCCGAGGGAGGAGTGGCGGTCATCAGTGGGACGAGCATGTC
GTGCCCCTTTGTCGGTTCGTCCTTCTCCTCGTTCGTTCGCTGAGCGTCGACTGATCTTGCACGATCGCAG
CTGGATCTGCGGCGGTCCTCATGGCCGCTCGCGCTTCGGAGAACCTCACGCCGCTTGAGATCAGGAGTCT
CCTTACTACCACTGCGAAGCTTACGCCGGTCTCGCTCTTGGGATCGACGCCTTTGGTGAGCGTGATTCGT
CAAGGAGGAGGTACGTTCCTTTCTCGCTGCTTTGAGGATCGAGGCTGATTAGCGAGCTTCGCAGGACTCG
TTCAGGTTGCCAAGGCGCTCGCGGCCAAGACGCTAATCTCTCCTCACGAGCTCCTGCTCAACGACACTGC
GAACGCGAACTACGTCCAGACTATCAAGATCAAGAACACCAACTCGTGGGCGATGAAGTACACCTTCTCC
TCGGCCGTCGCCCAAGGACTCGGAACTTTCGACGCTTCGGGCGATATCCTCCCTACCCTCGACCCAGTCG
CCGTCTCTGGCGCACAGGCTACCGTCGCGTTCAACACTCGGATCCTCAGCGTCGCGCCCGGCGCGACGGG
GTCCGTCGTGGCGACTATCACGCCGCCGGTTCTTCCCGTAGCGGACGCTGCGAGGTTCCCTATCTTCTCT
GGGTGGATCAGGGTGAATGGGCAAGGCGCGAGGGATAGCAGTAGGAACGAGGCGTACACTGGTGCGTTTG
GCTCGCTTTTTGGGAGGTGCCGAGCTAATCGTTTCTCGTAGTCCCGTACTTTGGGCTTGCGGCGAAGATG
ATCGATATGCAAGGTGCGTCGATGGTTCTCTCGCTTCTCTCTCGGTTGCTCACTTCAAAGTGCAGTCCTC
GACACCACCGAGACCATTTACGGTCCGGGCTACGCCTACCCCTTCGTGATCGACGACGCGATTGGAGACA
TCCAATCCACCACAACGTCGTACTCCAGGAACCTCGGGCCCACCGTCTTCGCTCGCTTTGCCATGCGTCG
ACCGTCCTCATTTCCTGCGGCAGAGCGAGAGCTGATTCCGACCTCGAACAGCTGGAACCCTTCACTACAG
CCTCGATCTCGTCCTAGCCGACATCGCCTTTACCCCCACCTACCCCAACTCCTCCCCCGCCACTCGTTTC
GTCAAGCGCTCCCTCACGCAGCACACCTCTGCCGCTTCGCACCTCGCCAAGCGCCGCGTCTCCATCGCCA
CTATCAACCCCAAAGCCACCCTCGTCGCCGATCGACAGCTCCACTCGGACGTCCCTATCGAGGGCAACAT
CTTCACCCAACCCTTTACTGGAAGGGATTACCTCGTCGACGCAGCCCCGACGGGATCCACCGATCGTACC
GTCACTTTTAACGGGCAGTACGCCGAGAACGGCCTCGTGAGGACGGCTGTGACGGGGACTTCGTACCGCT
TCCTCCTTCGGGCGTTGAAGATCTCGGGAGACGCGATGTACGAGGATCAGTATGAGAGCTGGCTCTCGCT
ACCGTTCTCGTTCCGTGCGTAG
91531 bp)
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Sequence overlapping: Both partial sequences from recombinant plasmid 4Blunt/PI12 protease (shotgun
cloning) and DNA walking were joined together and made up a total length of 2687 bp
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