Supplemental Table 1. Bacterial strains used in this study
Strain
name
DH10B
Description
E. coli strains
F- mcrA Δ(mrr-hsdRMS-mcrBC)
Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR
Δ(ara,leu)7697 araD139 galU galK nupG
rpsL λ-
Antibiotic
resistancea
Reference
None
Durfee et al., 2008
E1774
T-DNA binary vector Psuperpromoter-MCS/hptII
Kan
Lee et al., 2007
E2836
T-DNA binary vector P35SVirE2-YFP
Kan
Bhattacharjee et al. 2008
E3170
pSAT6-P35S-mRFP-NLS
Amp
Citovsky et al., 2006
E3187
E3458
E3480
pRTL2-P35S-mCherry
pSAT4-VirE2-cCFP
Amp
Amp
Amp
This study
This study
This study
E3567
pUC119-P35S-VirE2-cCFP+ P35S-VirE2nCerulean
Amp
This study
E4123
pSAT1-Pnos-VirE2-Venus
Amp
This study
E4126
Psuperpromoter-VIP1S79A in E1774
Kan
This study
E4127
Psuperpromoter-VIP1S79D in E1774
Kan
This study
E4130
E4145
Psuperpromoter-VIP1 in E1774
T-DNA binary vector pPZP-RCS2/hptII
T-DNA binary vector pPZP-hptII/PnosVirE2Venus
T-DNA binary vector pPZPnptII/P35SVirE2/mRFP-VirD2NLS
Kan
This study
Spe
This study
Spe
This study
Spe
This study
E4229
pSAT5-P35SVirE2
Amp
This study
E4231
pSAT1-PocsVIP1-Venus
Amp
This study
E4232
pSAT1-PocsVIP1S79A-Venus
Amp
This study
Amp
This study
Spe
This study
Spe
This study
Spe
This study
E4147
E4220
E4233
E4234
E4248
E4249
pSAT1-P35S-nVenus-VIP1
S79D
pSAT1-PocsVIP1
-Venus
T-DNA binary vector pPZP-nptII/
mRFP-VirD2NLS
T-DNA binary vector pPZPhptII/P35SVirE2/mCherry-VirD2NLS
T-DNA binary vector pPZP-hptII/
PocsVIP1-Venus/P35S-VirE2/mRFPVirD2NLS
E4250
E4251
E4257
E4258
E4259
E4260
E4261
E4262
E4263
A136
A208
GV3101
R1000
At849
At1811
T-DNA binary vector pPZP-hptII/
PocsVIP1S79A-Venus/P35S-VirE2/ mRFPVirD2NLS
T-DNA binary vector pPZP-hptII/
PocsVIP1S79D-Venus/P35S-VirE2/ mRFPVirD2NLS
T-DNA binary vector pPZP-hptII/
PocsVIP1-Venus/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII/
PocsVIP1S79A-Venus/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII/
PocsVIP1S79D-Venus/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII
PnosVirE2Venus/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII PnosVirE2Venus/P35SVIP1S79A/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII PnosVirE2Venus/P35SVIP1/mRFP-VirD2NLS
T-DNA binary vector pPZP-hptII PnosVirE2Venus/P35SVIP1S79D/mRFP-VirD2NLS
Agrobacterium strains
Non-tumorigenic; C58 cured of its Ti-plasmid
Tumorigenic; pTiT37 in A136
Non-tumorigenic, disarmed
Tumorigenic; pRiA4b in A136
Non-tumorigenic; GV3101 containing pBISN1
Tumorigenic; GALLS expressed in the virE2
mutant strain A. tumefaciens A348::mx358
At1922
pE2836 in GV3101
At1982
Tumorigenic; VirE1/E2 expressed in the virE2
mutant strain A. tumefaciens A348::mx358
At1983
pE4130 in E1774
At1984
pE4126 in E1774
At1985
pE4127 in E1774
At2055
pE4249 in GV3101
At2056
pE4250 in GV3101
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Spe
This study
Rif
Rif
Rif, gent
Rif
Rif, kan
Rif, carb,
kan, tet
Rif, gent,
kan
Rif, carb,
kan, tet
Rif, gent,
kan
Rif, gent,
kan
Rif, gent,
kan
Rif, gent,
spe
Rif, gent,
Watson et al., 1975
Sciaky et al., 1978
Koncz and Schell, 1986
White et al., 1985
Narasimhulu et al., 1996
Hodges et al., 2004
This study
Hodges et al., 2004
This study
This study
This study
This study
This study
At2057
pE4251 in GV3101
At2058
pE4257 in GV3101
At2059
pE4258 in GV3101
At2060
pE4259 in GV3101
At2065
E4260 in GV3101
spe
Rif, gent,
spe
Rif, gent,
spe
Rif, gent,
spe
Rif, gent,
spe
Rif, gent,
spe
This study
This study
This study
This study
This study
Rif, gent,
This study
spe
Rif, gent,
At2067 pE4262 in GV3101
This study
spe
Rif, gent,
At2068 pE4263 in GV3101
This study
spe
a
amp, ampicillin; car, carbenicillin; gent, gentamicin; kan, kanamycin; rif, rifampicin; spe,
spectinomycin; tet, tetracycline
At2066
pE4261 in GV3101