5
6
7
1
2
3
4
8
9
10
Supplementary Tables and figures
Table 1: Likelihood ratio test for selecting the optimal model. Each test compared the fit of pairs of nested models. Model column shows the term excluded in the nested model. The complete model included environmental treatments (Control, Water, Nutrient and W+T), population origin (KY-31, Åland, Gotland and Sweden), endophyte colonization status (E+, M- and E-) and their interactions on total plant biomass (g), number of tillers and seed mass (g) of Schedonorus phoenix plants. The columns show the likelihood ratio (L.ratio) and the associated probability (p-value).
Model Aboveground biomass
L.Ratio p-value L.Ratio
Tillers
p-value L.Ratio
Seed production
p-value
Environmental treatments (Et)
Population origin (P)
Endophyte colonization (Ec)
P x Et
Ec x Et
P x Ec
P x Ec x Et
-
-
24.92
21.23
10.61
10.57
29.66
-
-
<.0001
0.0116
0.1009
0.1025
0.0408
4.15
-
-
14.44
1.95
14.62
27.97
0.2453
-
-
0.1075
0.9242
0.0234
0.0625
33.39
39.18
9.94
5.26
16.41
8.97
16.41
<.0001
<.0001
0.0069
0.8103
0.5637
0.1751
0.5637
11
12
13
14
15
16
Table 2: Likelihood ratio test for selecting the optimal model. Each test compared the fit of pairs of nested models. Model column shows the term excluded in the nested model. The complete model included environmental treatments (Control, Water, Nutrient and W+T), population origin (KY-31, Åland, Gotland and Sweden), endophyte colonization status (E+, M- and E-) and their interactions on planicle/tiller ratio and reproductive effort of Schedonorus phoenix plants. The columns show the likelihood ratio (L.ratio) and the associated probability (p-value).
Model
L.Ratio
Panicles/Tillers
p-value L.Ratio
Reproductive effort
p-value
Environmental treatments (Et) 56.26 <.0001 14.49 0.0023
Population origin (P) - - 47.19 <.0001
Endophyte colonization (Ec)
P x Et
Ec x Et
P x Ec
P x Ec x Et
-
12.98
9.20
21.95
13.47
-
0.1631
0.1623
0.0012
0.7627
9.91
8.98
5.17
9.07
20.41
0.007
0.4383
0.5221
0.1692
0.31
17
18
19
20
21
Table 3: Likelihood ratio test for selecting the optimal binomial model. Each test compared the fit of pairs of nested models. Model column shows the term excluded in the nested model. The complete model included environmental treatments (Control, Water, Nutrient and W+T), population origin (KY-31, Åland, Gotland and Sweden), endophyte colonization status (E+, M- and E-) and their interactions on the incidence of
Claviceps spp. and plant survival of Schedonorus phoenix plants. The columns show the Chi-square (Chisq), the degrees of freedom (Df) and the associated probability (p-value).
Model
Chisq
Claviceps
Df Pr(>Chisq) Chisq Df
Survival
Pr(>Chisq)
Environmental treatments (Et) 5.23 3 0.155 14.15 3 0.002
Population origin (P) - - - 20.97 3 0.0001
Endophyte colonization (Ec)
P x Et
Ec x Et
P x Ec
P x Ec x Et
-
10.63
5.82
22.92
14.524
-
9
6
6
18
-
0.302
0.443
0.0008
0.694
0.82
10.22
5.11
10.19
8.16
2
9
6
6
18
0.660
0.332
0.528
0.116
0.976
22
23
24
25
26
27
28
29
30
Table 4: Analyses of variance for the effects of environmental treatment (Control, Water, Nutrient, and W+T), population origin (KY-31, Åland,
Gotland and Sweden), and endophyte colonization status (E+, M- and E-) on total plant biomass (g), number of tillers and seed mass (g) of
Schedonorus phoenix plants. Only the significance of the fixed factors and the interactions from the optimal model are reported.
Source
Intercept
Environment treatment (Et)
Population origin (P)
Endophyte colonization (Ec)
P x Et
Ec x Et
P x Ec
P x Ec x Et
9
-
-
1
3
3
2
-
Total aboveground biomass Tillers Seed mass numDF DenDF F-value P-value numDF DenDF F-value P-value numDF DenDF F-value P-value
413 674.19 <0.0001 1
27 21.28 <0.0001 -
413 3.53 0.015 3
413 12.53 <0.0001 2
413 2.23 0.018
- - -
- - -
-
-
6
- - - -
429 1172.06 <0.0001 1
- - - 3
429 12.81 <0.0001 3
429 32.14 <0.0001 2
-
-
-
-
429 2.40
-
-
0.027
-
-
-
- - - -
-
-
-
364
27
364
364
-
-
-
-
72.54
16.97
14.67
5.88
-
-
-
-
<0.0001
<0.0001
<0.0001
0.003
-
Table 5: Analyses of variance for the effects of environmental treatment (Control, Water, Nutrient, and W+T), population origin (KY-31, Åland,
Gotland and Sweden), and endophyte colonization status (E+, M- and E-) on panicle/tiller ratio and reproductive effort of Schedonorus phoenix plants. Only the significance of the fixed factors and the interactions from the optimal model are reported.
Source
Intercept
Environment treatment (Et)
Population origin (P)
Endophyte colonization (Ec)
P x Et
Ec x Et
P x Ec
P x Ec x Et
-
-
6
-
1
3
3
2 numDF
Panicle/tiller ratio
DenDF F-value
428
27
428
428
-
-
428
-
288.54
11.77
38.05
33.13
-
-
3.71
-
P-value
<0.0001
<0.0001
<0.0001
<0.0001
-
-
0.001
-
-
-
-
-
1
3
3
2 numDF
-
-
-
-
353
27
353
353
Reproductive effort
DenDF F-value
-
-
-
-
103.03
6.02
17.26
5.41
P-value
-
-
-
-
<.0001
0.003
<.0001
0.005