Journal of Comparative Physiology B
Title: Peroxiredoxin 6 from the emerald rockcod Trematomus bernacchii: characterization and transcription studies in
response to warming.
Authors: Tolomeo A.M.1§, Carraro A. 1§, Bakiu R.2; Toppo S.3, Place S.P.4, Ferro D.5, Santovito G.1*
Affiliations: 1Department of Biology, University of Padova, Padova, Italy; 2Department of Aquaculture and Fisheries,
Agricultural University of Tirana, Tirana, Albania, 3Department of Molecular Medicine University of Padova, Italy;
4
Department of Biology, Sonoma State University, Rohnert Park, CA, USA; 5Institute for Evolution and Biodiversity,
Westfälische Wilhelms-Universität, Münster, Germany.
§ Equally contributed
* Corresponding author.
via U. Bassi 58/B, 35100 Padova, Italy
Tel.: +390498276310; fax: +390498276300
E-mail address: gianfranco.santovito@unipd.it
Fig. S2: cDNA sequence of T. bernacchii Prdx6B and deduced amino acid sequence. Derived amino acid sequence
shown below coding sequence. 5′ and 3′UTR regions are underlined. Putative polyadenylation signal is highlighted in
gray. Start and stop codons are marked in blue. The catalytic centers for peroxidase (PVCTTE) and phospholipase
(GXCXG) activities are enclosed in green and orange boxes, respectively. The amino acid residues highlighted in green
represent the peroxidase triad. The amino acids of catalytic triad involved in phospholipase activity is highlighted in
yellow.
-1696
gtggtttcaaatattt
-1680 ttgttatctgtttataaatgttctctttatttaatttctcaataaaaaaagtgaaaataa
-1620 acctttttgtttcatatatttagtttatttaatgaaaaaaagtatgaccccaaggttgtg
-1560 tcaatcagtctatgtggtgtcagaaatggtgtatagaatattgatattttttaaggtatt
-1500 gtatccaagttagacattcttacacaaatagactgtataccaaatacattgttgggatga
-1440 tttaattatggtatttctaagtctattttattgttaccaaaacacagaatgtatcctaaa
-1380 aaaaaatctccatgctcatcatctgaacttatttactttacttagttgaactggtgtata
-1320 tacagaaattgtttggataaggcacttttgtgtagactccactgatagtgaaaaagagat
-1260 caaattgtgacggtatacaacagatttagtttgtatcttctccacatgcagtgatatcac
-1200 tattatatgtttggcatactatgccatgacaagtgatagtaggatgaaacatgaacgtga
-1140 atgaagtgtattgtgtattcaatataagactactgcattcacgaaagttagtttgaaagt
-1080 ttaagaggtgtttctgtgcgtaaagtcaaaaagtatccaaactggcgtgccacatttgaa
-1020 gggaaagtgctggaaattgttggaaaacgaactgaatatatacaaaaagacaatagttat
-960 ctgttggttcgcagaagtccaacccttttcttattggagtaattatctgagccaaaatca
-900 aagcagttcaagtcgctcacccagcgctaggttgaacatgatctcctgctcttccaaacc
-840 ctccccccggagaagagtgtctagcgctcagagagctccaccagccgcacaacagctgct
-780 ccatggagactcctcagaggcgcacactcccagcagcttggacagccagatacatcgcca
-720 gcagaaaccggagcgttatggagaatatcggctggctgagactgacggaaaagtcttcag
-660 gtggcgctccaaaggaagagtataagaacatctggagcaccggctgcggacatggcactc
-600 aggatgcccggttcccggaagatatatcttttggacattggtaaggacatttttatatgc
-540 caaagtgtcaacagtaatctatcaaaccgactttatcgatcacctgtggtcagaaaaaac
-480 gtatatcctgctctactgcgacgcacatacatcatgcaaccggtctggagtccggttaaa
-420 ggggtatacattaccatacattatcttggagttttaaacgtatgatttcttctttgaaat
-360 ctaaaacaaatgcactgcatctaggcaataaaagtaaatgttataattcaattgccgata
-300 atacatgagcatattatcgtgaagaagtgctgtgtttatacaaaatcctcaaccttatgt
-240 aggaatctggtctatacgcgtgcgtaaattggtcatttggaaacatcgagacaaccaatc
-180 aacgaaagcgcgccctgaacgcaccgcagagtcagatcatagtttattataggtgtcctg
-120 tgcaggcacagttgatgatgatgtaaatagagaagcctatgcagagggaagcttccgtct
-60 ctttatttaacgcatctgtgatcagagctgcacagagacgcattcacggtcactctgaac
1 atgccggggctgctgctcggagatgcgtttcctaacttcgaagcggagacgacaattgga
1 M P G L L L G D A F P N F E A E T T I G
61 aaaattaaactccatgaatttctcggtgattcatggggggtcctgttctcccaccccaga
21 K I K L H E F L G D S W G V L F S H P R
121 gactacaccccggtgtgcaccacagagctggggcgcgctgccagactgagcactgagttc
41 D Y T P V C T T E L G R A A R L S T E F
181 agtaaacgcaacgtcaaaatgatcgccctgtccatcgactgcctggaggatcaccacagc
61 S K R N V K M I A L S I D C L E D H H S
241 tggagcaaggacatcctggcatacaactgtgaggactcggcctgccgctcgctgcccttc
81 W S K D I L A Y N C E D S A C R S L P F
301 cccatcatagcggacagtaaacgggagctggcagtagctctgggcatgctggacccagat
101 P I I A D S K R E L A V A L G M L D P D
361 gagaaggataaagatggcctgccgctcactgcacgctgtgtgttcgtcatcggccctgac
121 E K D K D G L P L T A R C V F V I G P D
421 aaaaggctgaaactgtccatactctacccggcaacaaccggacgcaactttgacgagatt
141 K R L K L S I L Y P A T T G R N F D E I
481 ttgagagcagtggattctctccaggccacagcagggaaacgagtgtccacacctgcagac
161 L R A V D S L Q A T A G K R V S T P A D
541 tggaagcctggagactgtgtgatggttcccgccagcatgcctgaggaagaggcagccgct
181 W K P G D C V M V P A S M P E E E A A A
601 ttgtttccagccggtgtctacaccaaagacctgccctctgggaagaagtacctgcgctac
201 L F P A G V Y T K D L P S G K K Y L R Y
661 accccgcagcagtgagcacgacggagacacacccgacagtcaacactcatttatgtcaca
221 T P Q Q 721 gtcagtccagcagctctcaatctgggactacattgagtgctgctagtctgtgtatgcggt
781 agacaaaacgccttaagaaatgtgtaggattcttttttaaataaagtgattttgaagtat
841 gtacacctctgagaatcaaagcttactttcataatggggattgttttttgaaagaagggg
901 gaggcttgagataggaacatatattttgtttatatttaaagatgtagcaaacagtttatt
961 gtatacagcatggcagagaagcttttatttttgtaggatgatactatcagggataacata
1021 taattcaaatctagacgttattgtttaaccacttcctctattttctcaagaggaagtggt
1081 taaaggggctagttacacaaaggatggctgcgagaggaaatgcacgctattgctactctt
1141 tttgaggggcaactatacctcatgaaacacagtaaacgaatcagtgtttcaaatgtttga
1201 aggggccctatgttttggggggttttccctttcctttagtgtgttatataggtgtttgtg
1261 catgttaatggtctgca