SUPPLEMENTARY MATERIAL
Isolation and characterization of a biosurfactant-producing
Fusarium sp. BS-8 from oil contaminated soil
Muneer Ahmed Qazi, Tayyaba Kanwal, Nighat Fatima, Muniba Jadoon, Safia Ahmed
Critical micelle dilution (CMD) and critical micelle concentration (CMC) of partially
purified biosurfactants
The partially purified biosurfactants obtained after column chromatography were dissolved in
deionized water at an initial concentration of 10 mg ml-1 (w/v) and diluted 10-folds up to three
levels (i.e. 10x, 100x, and 1000x). The dilutions were labeled as CMD-1, CMD-2 and CMD-3,
respectively, and analyzed for surface tension value at room temperature by Whilmey plate
method using tensiometer (EasyDyne K20, KRUSS, Germany). Similarly, in order to find
critical micelle concentration, the surface tension of partially purified biosurfactant (5 mg ml -1,
w/v) was measured in ascending order of biosurfactant concentration (i.e. lower to higher) at
room temperature as described. All the measurements were done in triplicate; each value is
represented as mean ± SD, and expressed in mN m-1.
Table S- 1 Relationship between surface tension and critical micelle dilution (CMD) of the cell free broth of
Fuarium sp. BS-8 grown in MSM under optimized culture conditions.
Time
(days)
NC3
0
5
10
15
20
1
SFT±SD1
(mN m-1)
72.14±0.22a
56.71±0.15b
44.21±0.63c
33.24±0.35d**
32.11±0.08d**
32.16±0.64d**
CMD-1±SD2
(mN m-1)
72.32±0.25a
58.05±0.11b
47.32±0.02c
36.68±0.24d*
34.82±0.31e**
37.46±0.05d*
CMD-2±SD
(mN m-1)
72.54±0.05a
63.21±0.05b
54.25±0.52c
44.36±0.15d
38.15±0.22e*
41.63±0.15d
CMD-3±SD
(mN m-1)
72.61±0.25a
69.58±0.02b
62.41±0.05c
56.87±0.32d
55.04±0.57d*
63.34±0.42c
Average surface tension ± standard deviation (mN m-1) of triplicate experiments.
Average critical micelle dilution (CMD)± standard deviation (mN m-1) of triplicate experiments. CMD-1= 10x
dilution; CMD-2= 100x dilution; CMD-3= 1000x dilution.
3
Negative control = Un-inoculated mineral salts medium (MSM).
** Highly significant values at p<0.05.
2
Figure S- 1 Critical micelle concentration (CMC) of the crude biosurfactant extract of Fusarium sp. BS-8 in relation
to surface tension
Figure S- 2 Schematic representation of one-factor-at-a-time optimization strategy followed during this study