Updated March 9, 2011 Genome project submission form Required Fields Project type: . Single Species Project Metagenomic Project Contact’s First Name: …….Supapon…….. Contact’s Last Name: ……..Cheevadhanarak……… Contact’s E-mail: ……supapon.che@kmutt.ac.th……. Fill in one or more of Submitting Organization: King Mongkut’s University of Technology Thonburi, Thailand National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Chiangmai University, Thailand Kazusa DNA Research Institute, Japan …………………………………………………… URL (optional) Optional Sequencing Center: ……………………………………………………. URL: http://....................................................................... Optional Consortium name: ……………………………………………………. URL: http://....................................................................... Organism Name: ……Arthrospira (Spirulina) platensis………… Strain/isolate/breed: ……..C1……………………. Locus Tag Prefix: ………………..SPLC1…………………….. Source of DNA used for sequencing Source DNA available from Prof. Dr. Avigad Vonshak at the Jacob Blaustein Institute for Desert Research at Sede Boqer, Ben-Gurion University of the Negev, Israel. (avigad@bgumail.bgu.ac.il ) Optional Replicons : N 1 Name Genome Size (Mb) 6.089210 Type Chromosome Sequencing Method : Sequencing Technology : x x. xx. .ป .ป ABI Sanger 454 Solexa Other Sequencing Depth : ………. 28.5X................. Project URL : x http://........................................ Data URL (if different) ……………………….. . Estimated Genome Size: ……………6.089210………… Mb Brief description of the importance of this organism to the scientific world: Arthrospira platensis also called Spirulina platensis is a cyanobacteria found locally in alkaline salt lakes. Unlike the other A. platensis strains, A. platensis C1 performed a single colony with non-gliding ability and able to used for arsenic culture selection. Based on these abilities, A. platensis C1 is selected to be used in the lab for genetic study. Extensive studies in both lipid desaturation mechanism and physiological conditions for cell growth have been performed using this strain. Genome data of A. platensis C1 would complete strain information and further use for comparative study with the other available genomic data. Comment to database staff. Please indicate the specifics of this project. For example, whether you are sequencing DNA or mRNA/cDNA; whether you are sequencing a genome or just a locus; whether you examining one genome or comparing multiple isolates/strains (and whether annotation will be included. A. platensis C1 genomic DNA have been sequenced and estimated at 6.09 Mb in size and possessed as a single, circular chromosome. NOTE: This is the first draft sequence of A. platensis C1 genome. The sequences have been assembly into one circular genome with 63 contigs and 63 gaps remaining. “n” is represented for sequences in gaps. 46 of know gaps have been reported here while 17 of unknown gaps are still remaining in the circular genome. Gene annotation also included in this draft genome sequence. The order of each contig is consider as submitted order, however, the sequences and order will be replaced by the complete A. platensis C1 genome when it is available. The accession number of each ORFs will be preserved between this draft genome and the complete genome. 1 206210: contig of 206210 bp in length 206211 206310: gap of unknown length 206311 235491: contig of 29181 bp in length 235492 236713: gap of 1222 bp 236714 657629: contig of 420916 bp in length 657630 657729: gap of unknown length 657730 804128: contig of 146399 bp in length 804129 823978: gap of 19850 bp 823979 983672: contig of 159694 bp in length 983673 984328: gap of 656 bp 984329 1144486: contig of 160158 bp in length 1144487 1147716: gap of 3230 bp 1147717 1151176: contig of 3460 bp in length 1151177 1154563: gap of 3387 bp 1154564 1504850: contig of 350287 bp in length 1504851 1525896: gap of 21046 bp 1525897 1557047: contig of 31151 bp in length 1557048 1558373: gap of 1326 bp 1558374 1882268: contig of 323895 bp in length 1882269 1882368: gap of unknown length 1882369 1884528: contig of 2160 bp in length 1884529 1884628: gap of unknown length 1884629 1910667: contig of 26039 bp in length 1910668 1911472: gap of 805 bp 1911473 2055701: contig of 144229 bp in length 2055702 2055801: gap of unknown length 2055802 2072887: contig of 17086 bp in length 2072888 2072987: gap of unknown length 2072988 2074290: contig of 1303 bp in length 2074291 2074390: gap of unknown length 2074391 2106714: contig of 32324 bp in length 2106715 2107483: gap of 769 bp 2107484 2275702: contig of 168219 bp in length 2275703 2275732: gap of 30 bp 2275733 2290884: contig of 15152 bp in length 2290885 2291478: gap of 594 bp 2291479 2320890: contig of 29412 bp in length 2320891 2321137: gap of 247 bp 2321138 3121049: contig of 799912 bp in length 3121050 3121389: gap of 340 bp 3121390 3122978: contig of 1589 bp in length 3122979 3123143: gap of 165 bp 3123144 3164700: contig of 41557 bp in length 3164701 3165592: gap of 892 bp 3165593 3296544: contig of 130952 bp in length 3296545 3297943: gap of 1399 bp 3297944 3390913: contig of 92970 bp in length 3390914 3391988: gap of 1075 bp 3391989 3395997 3414664 3492975 3493235 3643389 3647660 3652767 3652797 3654308 3658416 3761739 3770168 3810882 3820692 3837479 3839363 3846714 3846814 3870815 3870986 3873825 3874664 4016095 4017056 4151743 4152816 4180861 4180961 4184230 4184330 4185560 4186131 4397860 4402028 4434193 4434845 4479897 4483458 4487090 4492837 4496475 4496999 4500254 4500354 4510823 4510923 4521423 4521441 4581872 4588044 4732111 4732211 4870791 4871304 4993141 4993809 5326560 5327475 5688239 5692378 5805804 5812403 5836215 5836315 5876212 5879324 3395996: contig of 4008 bp in length 3414663: gap of 18667 bp 3492974: contig of 78311 bp in length 3493234: gap of 260 bp 3643388: contig of 150154 bp in length 3647659: gap of 4271 bp 3652766: contig of 5107 bp in length 3652796: gap of 30 bp 3654307: contig of 1511 bp in length 3658415: gap of 4108 bp 3761738: contig of 103323 bp in length 3770167: gap of 8429 bp 3810881: contig of 40714 bp in length 3820691: gap of 9810 bp 3837478: contig of 16787 bp in length 3839362: gap of 1884 bp 3846713: contig of 7351 bp in length 3846813: gap of unknown length 3870814: contig of 24001 bp in length 3870985: gap of 171 bp 3873824: contig of 2839 bp in length 3874663: gap of 839 bp 4016094: contig of 141431 bp in length 4017055: gap of 961 bp 4151742: contig of 134687 bp in length 4152815: gap of 1073 bp 4180860: contig of 28045 bp in length 4180960: gap of unknown length 4184229: contig of 3269 bp in length 4184329: gap of unknown length 4185559: contig of 1230 bp in length 4186130: gap of 571 bp 4397859: contig of 211729 bp in length 4402027: gap of 4168 bp 4434192: contig of 32165 bp in length 4434844: gap of 652 bp 4479896: contig of 45052 bp in length 4483457: gap of 3561 bp 4487089: contig of 3632 bp in length 4492836: gap of 5747 bp 4496474: contig of 3638 bp in length 4496998: gap of 524 bp 4500253: contig of 3255 bp in length 4500353: gap of unknown length 4510822: contig of 10469 bp in length 4510922: gap of unknown length 4521422: contig of 10500 bp in length 4521440: gap of 18 bp 4581871: contig of 60431 bp in length 4588043: gap of 6172 bp 4732110: contig of 144067 bp in length 4732210: gap of unknown length 4870790: contig of 138580 bp in length 4871303: gap of 513 bp 4993140: contig of 121837 bp in length 4993808: gap of 668 bp 5326559: contig of 332751 bp in length 5327474: gap of 915 bp 5688238: contig of 360764 bp in length 5692377: gap of 4139 bp 5805803: contig of 113426 bp in length 5812402: gap of 6599 bp 5836214: contig of 23812 bp in length 5836314: gap of 100 bp 5876211: contig of 39897 bp in length 5879323: gap of 3112 bp 5906861: contig of 27538 bp in length 5906862 5912876 5991942 5994195 6042385 6042485 6044313 6044413 6048273 6048373 6089111 5912875: gap of 6014 bp 5991941: contig of 79066 bp in length 5994194: gap of 2253 bp 6042384: contig of 48190 bp in length 6042484: gap of unknown length 6044312: contig of 1828 bp in length 6044412: gap of unknown length 6048272: contig of 3860 bp in length 6048372: gap of unknown length 6089110: contig of 40738 bp in length 6089210: gap of unknown length.