SOUTH AUSTRALIAN WILDLIFE ETHICS COMMITTEE APPLICATION TO UNDERTAKE STANDARD FAUNA SURVEYS IN SOUTH AUSTRALIA This form is to be used for standard vertebrate monitoring and baseline surveys only. Please refer to Guidelines for Vertebrates Surveys in South Australia for reference to standard surveys. Please complete this form and ensure that all applicants have signed the declaration. Scan and email the completed application electronically by Word or PDF format to: DEWNR.AnimalEthicsCommittee@sa.gov.au. If you have any questions please contact the Executive Officer by email at the address above or telephone: 08 8222 9435. Office Use Only Project Number: Date Received: Revision Number: Title of Project Name of Primary Applicant Summary of Animals Required Procedure Types (Refer Q8) Pain Classifications (Refer Q9) Expected date for animal use to begin? Expected date for animal use to end? Project duration? Does this application relate to a previously approved Protocol? Yes No Yes No If so, give previous approval number(s) Have reports on these projects been lodged? Does this project involve any of the following: Funds Source (Grant ID is applicable) Honours Student Masters Student PhD Student 1 Approval to share information By submitting this application I give approval for this application and any information relating to it to be shared by South Australian Animal Ethics Committees and the Animal Welfare Unit within the Department of Environment, Water and Natural Resources for the purposes of administration, approval and monitoring. Yes Is there any actual or potential interest, including financial interest or other relationship or affiliation by any research/team member involved in the project that may affect judgements and decision regarding the wellbeing of the animals involved? See Australian code for the care and use of animals for scientific purposes (The Code) Clause 2.7.4 [xxi] Declaration of interest Yes No If yes, outline the potential and any steps to be taken to ensure the ethical integrity of the project. Primary Applicant Reminder: All Honours/Masters and PhD studies must be approved by an AEC. The applicant must be the degree candidate supervisor. Name (include title) Applicant's Institution and Department Contact details (including After Hours) Email Phone Mobile Correspondence to Co-applicant/s If semi-permanent pitfall traps are to remain in the ground after the expiry of this approval please nominate the landholder or a company representative who will be responsible for routine checks and maintenance of these traps between survey events. Name (include title) Institution and Department Email Contact details (including After Hours) Phone Mobile 2 Other Applicant/s Name (include title) Institution and Department Contact details (including After Hours) Email Phone Mobile Name (include title) Institution and Department Email Contact details (including After Hours) Phone Mobile 1. Short Non-Technical Summary (Maximum 100 words) This short summary is useful to the Wildlife Ethics Committee (WEC) as a snapshot of your project. Please include the aims of the survey and the main types of field activity. 2. Provide a non-technical description of the project and its aims, and its hypothesis Completion of this answer must be clear and understandable by all WEC members including non-scientists. Please use language that avoids scientific and clinical terminology. Provide Aims, methods and previous information that you are building on. 3. Detailed Description of What Will Happen to the Animals 3.1 Using Lay Language describe what happens to the animals from the time they are obtained until the time the project is completed. Provide a clear step by step description of what will happen to the animals, for complex projects consisting of many steps, a brief paragraph outlining the whole proposal should be included, with the details of each step provided sequentially. What the animals will experience as a result of this research project from the time the animals are caught until the time they are released or killed e.g. handling animals for identification, animals that will be kept and euthanized for voucher specimens, genetic sampling, transporting, or tagging or marking animals. 3 3.2 Where will the procedures/use of animals take place? (If more than one location is to be used, please attach a map and clearly explain what will happen at each different site) Attach a map of the area to be surveyed or describe the location e.g. area of X km square located south of YYYY Procedure/Use of Animals Locations (Nearest Named Place) 3.3 Please append a list of species already recorded within the survey area. Indicate which of these species is represented by verifiable museum specimens. Use the following table layout. Species Number of records Represented by voucher specimens (yes/no) 4. Capture and Use of Wildlife Please answer each question in full detail. It is not sufficient to enter ‘as per Standard Operating Procedure (SOP) – see attachment’. You must describe your procedures. If a SOP or advisory document is available use your application to note that it has been consulted and your approach is compatible with those guidelines, or explain why you need to deviate from any standard approach. 4.1 Capture Methods As a starting point, refer to the Guidelines for Vertebrate Surveys in South Australia that outline a standard approach to general fauna survey that has proven to be effective. However the WEC understands that particular surveys may need to vary their approach. See also Biological Survey of South Australia. 4.1.1 What types of traps will be used? For standard commercially produced traps simply state the maker and model of each trap type. Where the trap is not commercial or a standard device please describe it in full (append diagram). 4.1.2 How many traps will be set and over what period of time? 4 4.1.3 How often and at what times will traps be checked and/or cleared? Please refer to the WEC Use of live traps to capture terrestrial vertebrates policy 4.1.4 How will the traps be identified and their locations recorded? 4.1.5 How will distress and death of trapped animals be minimised? (Hot, cold or wet weather etc.) Please state the critical temperature range/climatic extremes at which time the trapping will cease. 4.1.6 How will predation of trapped animals be minimised? (Ants, crows etc.) Provide details on how you will protect the captured animals from ants, crows etc. and animals that may be captured in the same trap e.g. place sand and leaves in the pitfall, use insecticide around the trap line. Will you relocate traps if ants are a problem? 4.1.7 Are semipermanent pitfalls part of the survey procedure? If so how will traps be inactivated when not in use, and maintained/monitored between survey periods? The person responsible for maintenance/monitoring of traps between survey periods must be named as a coapplicant. 4.1.8 Describe your procedures and timing of the release of captured animals? (E.g. dealing with nocturnal animals) 4.1.9 If bait is used or food/water provided in traps give details. 4.1.10 What is the maximum number of traps each team leader will have responsibility for within a trapping period? How many assistants will provide support? Please provide an estimate so that the workload can be demonstrated to be consistent with good monitoring of animal welfare. 4.1.11 Describe any other methods to be used for capture e.g. Mist netting, spotlighting and hand foraging. 4.1.12 What established standard operating procedures or recognised guidelines (relevant to animal welfare) will be followed? Attach a copy of any relevant documents at the end of this document. For further information visit Policies for wildlife research. 5 4.2 Collection of Museum Voucher Specimens The WEC considers that the collection of voucher specimens is a component of a best practice approach to documenting and permanently verifying the distribution of vertebrate species within South Australia. Prior to any survey, applicants must establish the current state of knowledge of vertebrate diversity within the survey area. From this they would be expected to identify what species might be required as vouchers. Typically WEC would expect that any species newly recorded for the area would be vouchered, as would any animals where identity was uncertain. The SA Museum holds the state’s reference wildlife collections and can be consulted to clarify the current state of vertebrate specimen records from the proposed survey area or a species list of all specimen records that have been vouchered may be obtained from the Atlas of Living Australia website. Voucher specimens are to be preserved using standard museum methods. Applicants are encouraged to contact their local state museum for advice regarding preservation techniques for both animals and tissue samples. Policies for wildlife research In the case of monitoring surveys where the fauna of a region has already been well documented through baseline surveys, vouchering can be replaced by tissue samples and photography (section 4.3) Are voucher specimens to be collected? Yes No, Move to Q4.3 4.2.1 Provide a list of species already represented in museum collections (available from the Atlas of Living Australia or SA Museum) 4.2.2 Describe the method of euthanasia for animals that are selected as voucher specimens 4.2.3 How will the animals and associated tissue samples be preserved? E.g. Formalin, liquid nitrogen, ethanol or ethanol/saline. Provide details 4.2.4 Where will the voucher specimens be lodged? If not the South Australian Museum, please state which institution the specimens will be lodged. 4.3 Collection of biological samples (e.g. hair, tissue, blood etc.) Are biological samples to be collected? Yes No, Move to Q4.4 Policies for wildlife research Tissue-only samples should be accompanied by a photograph sufficient to identify the specimen from which the sample was taken. Photographs should accompany the specimens when they lodged at their final repository (Museum, Department and Office) 4.3.1 What samples (including blood, tissue, hair, feather, swab etc.) will be collected and how will these be taken? 6 4.3.2 What size/volume/amount of sample will be collected from each individual animal? For blood, express this as a percentage of the animal's circulating blood volume. 4.3.3 What blood/tissue collection route, needle size, technique(s) and equipment will be used? 4.3.4 How will pain during the procedure be minimised? 4.3.5 How will the risk of infection at the site be minimised? 4.3.6 How will animals be restrained during handling and/or sampling? 4.4 Transporting Animals Yes No, Move to Q4.5 In this section briefly outline why you will need to transport live animals. This includes animals that will be transported back to base camp and later released or euthanised. Refer to the link below for all Policies and guidelines for Wildlife Research which includes the Guidelines for transportation of live animals Policies for wildlife research 4.4.1 Is transport of live animals necessary and if so what method and precautions will be used? 4.4.2 What is the type of container to be used? 4.4.3 What shelter/bedding will be provided? 4.4.4 How many animals per container? 4.4.5 Will food and/or water be provided? Give details. 4.4.6 What precautions will be taken to protect animals from temperature extremes? 4.4.7 What is the maximum length of time that animals will be held in this way? 7 4.5 Identification of Individual Animals (e.g. photo, microchip, paint or ear tag) Yes No, Move to Q5 Individual identification is not always required for survey work. If you do need to identify individuals use this section to provide background information why it is necessary and make clear the time frame over which the identification needs to last. 4.5.1 How will animals be individually identified? 4.5.2 If animals will be marked temporarily or permanently, describe how this will be done. 4.5.3 If animals are to be marked permanently, give evidence that the potentially negative consequences of any marking technique are outweighed by the benefits gained by the use of this technique in your research. 4.5.4 Animals should only be marked permanently when a project is sufficiently funded to ensure that efforts can be made to recapture/relocate the marked animal/population. Explain whether there is such funding. 5. Management of Unexpected Adverse Events This section does not apply to animals that are sick or injured through natural causes. It applies strictly to healthy animals that have been impacted by the actions of the researcher. 5.1 What will happen to an animal that has been injured as a result of your survey activities? 5.2 If an animal needs to be euthanised in an emergency, how will this be done, and by whom? It is expected that the persons carrying out fauna survey work be equipped with the relevant euthanising agents and would have the necessary training and permits to be self-sufficient. If euthanasia is to be conducted by a veterinarian – how will an after-hours emergency euthanasia be obtained? Please state the name of the veterinarian, the practice and the location. 5.3 Identify any other relevant emergencies that may arise (e.g. whilst working in the field in remote or inaccessible locations) and explain what procedures are in place to deal with those emergencies. 6. Monitoring of Potential Pain and Distress 6.1 Identify and justify all procedures with potential to cause pain or distress. What steps will be taken to avoid or minimise such pain or distress? 8 6.2 Detail the monitoring that will be made of any animals not immediately released at point of capture. 7. Is the acquisition, retention or use of the animals subject to any permit, law or regulation of the State or Commonwealth? Yes No e.g. your licence number for teaching, research or experimentation in South Australia under the Animal Welfare Act 1985, Scientific research permit number under the National Parks and Wildlife Act 1975, Protected native or imported species; Australian Bird and Bat Banding Scheme (ABBBS) Banding Authority; Ministerial Exemption under Section 115 of the Fisheries Management Act 2007, SA Health Department Controlled Substances Licence (if you will be euthanising animals as voucher specimens). If yes, please provide details of the permit/authorisation number(s) and holder(s). 8. Procedure Category (cross all appropriate categories) Observational Studies: e.g. monitoring surveys, live trapping, obtaining weights and body measurements. Minor Conscious Intervention: No Anaesthesia: e.g. leg-banding, blood sampling, tissue clipping for genetic sampling purposes, without anaesthesia. 9. Pain/Distress Classifications (cross where appropriate) Category Extent and Duration Suffering Procedures No pain or distress Mild pain or distress 10. Substances to be administered 10.1 Humane Killing Agents Drug (generic ± brand name) Dose Rate (mg/kg) Concentration (mg/ml) Dose/Volume Required (mg/ml) Route of Administration Needle Size 10.2 Inhalational Agents (eg gaseous anaesthetics) Drug (generic ± brand name) Delivery Gas & Flow Rate Concentration Delivered (%) for Induction Concentration Delivered (%) for Maintenance Delivery Method Possible adverse effects of administration or withdrawal 9 10.3 What training and experience do you have in using these agents? (Note that any required licenses to hold and administer the agents listed above must be outlined in Section 9.) 11. Please discuss the ethical issues that the AEC will need to consider when reviewing this proposed survey. Your answer should address the 3Rs, Replacement, Reduction & Refinement. Report any streamlining or alteration of survey procedures that you are using that improve animal welfare (See Clauses 1.18–1.30 of the Code). “Not applicable” is not acceptable. 1. Ethical Issues e.g.: What is the welfare cost to the animal? In what way is the level of pain/discomfort justified? How does this mesh with the cost/benefit 2. Reduction: 3. Refinement: 4. Replacement Consideration of Alternatives Your response, should include the following: A list of any potential alternatives to animal use Whether any of these alternatives would be used Details of literature searches you have undertaken This answer should explain why animals need to be used at all. 12. In what way does this proposal relate to your previous or concurrent work? It is useful for the AEC to understand how this proposal fits into the broad research strategy/interest of your group. 10 13. Publications and Reports 13.1 List any publication and reports that have been completed for past wildlife ethics projects. 13.2 Have progress and final reports for past wildlife ethics approvals been lodged? 14. Do you propose to publish the results and/or make your data available to the wider community? If not, please explain why. 15. How will you minimise health risks posed to animals captured during the survey? E.g. transmission of pathogens or parasites from one animal to another? 16. What health risks to other staff exist? How could staff be affected? 17. Credentials of all those involved in the project Name and Qualification Detail the experience each participant has in the procedures to be undertaken with the species being used (if no experience, describe how relevant training and supervision will be obtained In which procedure(s) is this person involved? Date this person attended an Animal Users Training Day? Chief Applicant(s): Other People Participating 18. Dual/multiple AEC approval Is approval by more than one AEC required? Yes No If so, which AEC(s)? 11 19. Attachments Summary Checklist Type Attachment 1. Relevant Standard Operating Procedures (SOPs) Relates to Q4.1.14 Yes No 2. Publications Yes No 4. Other Yes No Please detail: 12 20. Declaration Project Title: Section 1: Declaration by the Primary Applicant I hereby declare that: (i) (ii) (iii) (iv) (v) I and all others involved in this project are familiar with and will comply with the relevant Commonwealth and State or Territory legislation and the requirements of the Australian Code of Practice for the care and use of animals for scientific purposes, 8th Edition 2013 (The Code) To the best of my knowledge this proposal conforms to the Code (8th Edition 2013) and the South Australian Animal Welfare Act 1985. I have read Section 2 of the Code which sets down the responsibilities of investigators. I accept responsibility for the conduct of all procedures detailed in this application and for the supervision of all personnel delegated to perform any such procedures. I agree to comply with procedures described and any conditions imposed by the Animal Ethics Committee. Sufficient and adequate resources will be available to undertake the proposed study. Primary Applicant's Name Primary Applicant's Signature Date Section 2: Other Applicant's Declaration I hereby declare that: (i) (ii) I am familiar with and will comply with the relevant Commonwealth and State or Territory legislation and the requirements of the Australian Code of Practice for the care and use of animals for scientific purposes, 8th Edition 2013 (The Code) and the South Australian Animal Welfare Act 1985 and its regulations. I have read the application and I accept the responsibilities detailed therein to the extent of my involvement in this project. Other Applicant's Name Other Applicant's Signature Date 13 Section 3: Declaration by the Institutions Nominated Authority (not the Primary Applicant) i.e. Supervisor/Head of Division/Department /Institute I hereby declare that: (i) (ii) (iii) I am satisfied that the Primary Applicant has the appropriate qualifications and experience to carry out the work with minimum distress to the animals. I believe this work meets the requirements of the Australian Code of Practice for the care and use of animals for scientific purposes, 8th Edition 2013 (The Code) and the South Australian Animal Welfare Act 1985 and its regulations. I have read the application and I am satisfied that this work is of sufficient scientific merit for my Department to be involved in it and sufficient and adequate resources will be available to undertake the proposed study. Nominated Authority Signature Date AEC Use Only Date Received: Action Taken: Signature of Chair, AEC Date of AEC meeting for consideration: 14 Attachment: Relevant Standard Operating Procedures (Question 4.1.14) Attach as pdf pages or if text, by copy-paste into field below 15