A Unique Preliminary Study on Placental Apoptosis in Mice with Passive
Immunization of Anti-Phosphatidylethanolamine Antibodies and AntiFactor XII Antibodies
By:Velayuthaprabhu, S (Velayuthaprabhu, Shanmugam)[ 2,3 ] ; Matsubayashi, H (Matsubayashi, Hidehiko)[ 1,3 ] ; Sugi,
T (Sugi, Toshitaka)[ 3,4 ] ;Nakamura, M (Nakamura, Masato)[ 3,5 ] ; Ohnishi, Y (Ohnishi, Yasuyuki)[ 5 ] ; Ogura, T (Ogura,
Tomoyuki)[ 5 ] ; Tomiyama, T (Tomiyama, Tatsuhiro);Archunan, G (Archunan, Govindaraju)[ 2 ]
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume: 66
Issue: 5
Pages: 373-384
DOI: 10.1111/j.1600-0897.2011.01008.x
Published: NOV 2011
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Abstract
Problem
Antiphospholipid antibodies have been investigated both in humans and in animal models. In contrast, there are fewer
reports describing anti-phosphatidylethanolamine (aPE) antibodies in humans, and there are no reports of animal
studies with aPE till date. Clinically, FXII deficiency or anti-FXII antibodies are sometimes associated with aPE in
patients with recurrent pregnancy loss. Therefore, we asked whether aPE and/or anti-FXII in mice could cause fetal
resorption, placental thrombosis and apoptosis. Moreover, antibodies to respective target antigens (LDC27 or IPP30)
could cause pregnancy failure as well.
Methods of study
Animal models were used to carry out these objectives. All the animals were immunized with different antibodies by
passive immunization. Placental samples were used for various observations.
Results and Conclusions
Mice with passive immunization of aPE (or anti-LDC27) and aFXII (or anti-IPP30) produced a slight increase in fetal
resorption, but markedly induced thrombosis and hemorrhage in the placenta associated with lower platelet counts and
increased placental apoptosis. In addition, fewer mitotic cells, less trophoblast giant cell invasion, and more shrunken
cells in the deciduas were seen. Our study supports the pathogenic role of aPE and aFXII in pregnancy complications
and also suggests a beneficial role of LDC27 and IPP30 antigens on pregnancy failures.
Keywords
Author Keywords:aFXII; aPE; APS; placental apoptosis; pregnancy loss
KeyWords Plus:EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME; COAGULATION-FACTORXII; INTRAUTERINE GROWTH RESTRICTION;SYSTEMIC-LUPUS-ERYTHEMATOSUS; RECURRENT PREGNANCY
LOSSES; IX-V COMPLEX; ANTIPHOSPHATIDYLETHANOLAMINE ANTIBODIES;ANTICARDIOLIPIN
ANTIBODIES; BINDING-SITE; NAIVE MICE
Author Information
Reprint Address: Matsubayashi, H (reprint author)
Osaka New ART Clin, Kita Ku, Pacific Marks Nishiumeda 10F,2-6-20 Umeda, Osaka 5300001, Japan.
Addresses:
[ 1 ] Osaka New ART Clin, Kita Ku, Osaka 5300001, Japan
[ 2 ] Bharathidasan Univ, Dept Anim Sci, Tiruchchirappalli, Tamil Nadu, India
[ 3 ] Tokai Univ, Dept Obstet & Gynecol, Sch Med, Hachioji, Tokyo, Japan
[ 4 ] Sugi Womens Clin, Yokohama, Kanagawa, Japan
[ 5 ] Cent Inst Expt Anim, Miyamae Ku, Kanagawa, Japan
E-mail Addresses:matsubayashihide@me.com
Funding
Funding Agency
Grant Number
Matsumae International Foundation, Japan
Council of Scientific and Industrial Research (CSIR), New Delhi
CSIR-SRF (09/475 (0137)/2008-EMR-I dt.15/03/2008
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Publisher
WILEY-BLACKWELL, COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
Categories / Classification
Research Areas:Immunology; Reproductive Biology
Web of Science Categories:Immunology; Reproductive Biology
Document Information
Document Type:Article
Language:English
Accession Number: WOS:000296031500003
PubMed ID: 21623987
ISSN: 1046-7408
Other Information
IDS Number: 835JA
Cited References in Web of Science Core Collection: 55
Times Cited in Web of Science Core Collection: 9