Fig. S1
A
E.coli S17-1
R. solanacearum GMI1000
pYJ27
Up1 fabG1 Dn1
CH
Up1 Gm Dn1
fabG2
MP
CH
Conjugation!!
Up1 fabG1 Dn1
merodiploid!!
E.coli S17-1
Up1 Gm Dn1
CH
fabG2
MP
Conjugation!!
Up1 fabG1 Dn1
merodiploid!!
Up1 Gm Dn1
CH
fabG2
MP
fabG1
pYJ33
Up1 Gm Dn1
RS-G5 (fabG1::Gm/pYJ33)!!
fabG1
CH
fabG2
MP
pYJ33
fabG1
pYJ33
B
E.coli S17-1
R. solanacearum GMI1000
fabG2
pYJ32
Up1 EcfabG Gm Dn1
MP
Up1 fabG1 Dn1
CH
Conjugation!!
fabG2
merodiploid!!
Up1 fabG1 Dn1
MP
Up1 EcfabG Gm Dn1
CH
fabG2
RS-G3 (fabG1::EcfabG)!!
Up1 EcfabG Gm Dn1
CH
CH
C
E.coli S17-1
R. solanacearum GMI1000
fabG1
pYJ30
Up2 Gm Dn2
Up2 fabG2 Dn2
Conjugation!!
fabG1
merodiploid!!
Up2 fabG2 Dn2
CH
Up2 Gm Dn2
MP
fabG1
CH
RS-G2 (fabG2::Gm)!!
Up2 Gm Dn2
MP
Fig. S1. Strategy for isolation R. solanacearum fabGs mutant strains. Panels A,
isolation mutant strain RS-G5 (fabG1::Gm/pYJ33). Panel B, isolation mutant
strain RS-G3 (fabG1::EcFabG). Panels C, isolation mutant strain RS-G2
(fabG2::Gm). Abbreviations: CH, chromosome; MP, megaplasmid; Up1, the
upstream fragment of fabG1; Dn1, the downstream fragment of fabG1; Up2, the
upstream fragment of fabG2; Dn2, the downstream fragment of fabG2.