Fig. S1 A E.coli S17-1 R. solanacearum GMI1000 pYJ27 Up1 fabG1 Dn1 CH Up1 Gm Dn1 fabG2 MP CH Conjugation!! Up1 fabG1 Dn1 merodiploid!! E.coli S17-1 Up1 Gm Dn1 CH fabG2 MP Conjugation!! Up1 fabG1 Dn1 merodiploid!! Up1 Gm Dn1 CH fabG2 MP fabG1 pYJ33 Up1 Gm Dn1 RS-G5 (fabG1::Gm/pYJ33)!! fabG1 CH fabG2 MP pYJ33 fabG1 pYJ33 B E.coli S17-1 R. solanacearum GMI1000 fabG2 pYJ32 Up1 EcfabG Gm Dn1 MP Up1 fabG1 Dn1 CH Conjugation!! fabG2 merodiploid!! Up1 fabG1 Dn1 MP Up1 EcfabG Gm Dn1 CH fabG2 RS-G3 (fabG1::EcfabG)!! Up1 EcfabG Gm Dn1 CH CH C E.coli S17-1 R. solanacearum GMI1000 fabG1 pYJ30 Up2 Gm Dn2 Up2 fabG2 Dn2 Conjugation!! fabG1 merodiploid!! Up2 fabG2 Dn2 CH Up2 Gm Dn2 MP fabG1 CH RS-G2 (fabG2::Gm)!! Up2 Gm Dn2 MP Fig. S1. Strategy for isolation R. solanacearum fabGs mutant strains. Panels A, isolation mutant strain RS-G5 (fabG1::Gm/pYJ33). Panel B, isolation mutant strain RS-G3 (fabG1::EcFabG). Panels C, isolation mutant strain RS-G2 (fabG2::Gm). Abbreviations: CH, chromosome; MP, megaplasmid; Up1, the upstream fragment of fabG1; Dn1, the downstream fragment of fabG1; Up2, the upstream fragment of fabG2; Dn2, the downstream fragment of fabG2.