Table S1. Primers sets used for RT-PCR and pyrosequencing
Product
Genes
Primers
Sequence (5’-3’)
Application
Size
Forward
GGAAGACCACTCGCATTCCTT
PPAR γ
Reverse
GTAATCAGCAACCATTGGGTCA
Forward
TTCCAATCCATGTCAAAACCGT
Cox2
Reverse
Forward
Reverse
GTCACTGGTCAACTCCAGCAC
Forward
GGAGGTGGTGATAGCCGGTAT
Reverse
TGGGTAATCCATAGAGCCCAG
Forward
GGAGTGTTCGTGGGCTTAGG
Adiponectin
Reverse
GCAGCTCCGGTGATATAGAGG
Forward
AGGTCGGTGTGAACGGATTTG
GAPDH
Forward
Reverse
76bp
RT-PCR
97bp
RT-PCR
140bp
RT-PCR
140bp
RT-PCR
95bp
RT-PCR
134bp
Pyro-
122bp
sequencing
GCGGGAACGCAACAACATC
FAS
PPAR γ
RT-PCR
AGTCCGGGTACAGTCACACTT
C/ EBP α
Reverse
121bp
GGGGTCGTTGATGGCAACA
GTTGTTTGTTGTTTTGGTAAGATTT
BiotinATCTCTACTCTAATAATTCCAACTA
Sequencing
CTATTTGGTAAGATTTGGTATATTATAA
Table S2. Relative fat composition of gonadal and perirenal white adipose tissue (WAT) at PND60
in the offspring and grand-offspring
F1
F- PAH
F- Control
M- PAH
M- Control
(n=7)
(n=8)
333.96 ±
181.96 ±
55.64
95.66
58.23 ±
46.09 ±
9.05
13.04
M- PAH
M- Control
(n=9)
(n=9)
139.00 ±
97.67 ±
53.97
37.59
148.57 ±
126.96 ±
12.99
41.85
p-value
Offspring
(n=10)
(n=10)
202.25 ±
147.74 ±
p-value
Gonadal
WAT
<0.05
47.56
17.01
92.52 ±
68.96 ±
<0.01
(mg)
Perirenal
WAT
0.21
33.58
13.91
F- PAH
F- Control
0.06
(mg)
F2
p-value
Offspring
(n=8)
(n=8)
160.52 ±
110.12 ±
p-value
Gonadal
WAT
<0.05
50.71
40.32
110.38 ±
96.30 ±
0.10
(mg)
Perirenal
WAT
0.50
35.64
14.11
0.05
(mg)
Control, offspring following prenatal control exposure; PAH, offspring following prenatal PAH over-exposure
F-female, M- male, WAT were weighed and averaged for each group.
Mice were selected at random to across each litter, sex and batch of exposure. Data are presented as mean±SD. Mann
Whitney U test for significance was performed.