Supplemental data
Structures, DNA binding, DNA cleavage and antitumor investigations of a series of
molybdenum(VI) complexes with some N(4) methyl and ethyl thiosemicarbazone ligands
MOUAYED A. HUSSEIN, TEOH S. GUAN*, ROSENANI A. HAQUE,
MOHAMED B. KHADEER AHAMED and AMIN M.S. ABDUL MAJID
Figure S1. 1H NMR spectrum of 1.
1
Figure S2. 13C NMR spectrum of 1.
Figure S3. 1H NMR spectrum of 2.
2
Figure S4. 13C NMR spectrum of 2.
Figure S5. 1H NMR spectrum of 3.
3
Figure S6 . 13C NMR spectrum of 3.
Figure S7 . 1H NMR spectrum of 4.
4
Figure S8 . 13C NMR spectrum of 4.
Figure S9. 1H NMR spectrum of 1a.
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Figure S10 . 13C NMR spectrum of 1a.
Figure S11. 1H NMR spectrum of 2a.
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Figure S12. 13C NMR spectrum of 2a.
Figure S13. 1H NMR spectrum of 3a.
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Figure S14. 13C NMR spectrum of 3a.
Figure S15. 1H NMR spectrum of 4a.
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Figure S16. 13C NMR spectrum of 4a.
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Figure S17. ORTEP diagram of the crystal structure of 1.
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Figure S18. ORTEP diagram of the crystal structure of 3.
Figure S19. ORTEP diagram of the crystal structure of 4.
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Figure S20. ORTEP diagram of the crystal structure of 1a.
Figure S21. ORTEP diagram of the crystal structure of 4a.
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Figure S22. UV spectra of 3a and 4a. The arrows show the changes in absorbance upon
increasing amounts of CT DNA.
Figure S23. Plots of [DNA]/(εa–εf) vs [DNA]: A = 1a, B = 2a, C = 3a and D = 4a.
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S24. Fluorescence spectra of 3a and 4a. The arrows show the changes in fluorescence
intensity upon increasing amounts of CT DNA.
Figure S25. Agarose gel electrophoresis patterns of pBR 322 (0.025 µM) increasing
concentrations (1.0 µM – 6.0 µM) of ligands 3 and 4 and their complexes 3a and 4a.
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Figure S26. Crystal packing structure of 1.
Figure S27. Crystal packing structure of 3.
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Figure S28. Crystal packing structure of 4.
Figure S29. Crystal packing structure of 1a.
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Figure S30. Crystal packing structure of 2a.
Figure S31. Crystal packing structure of 3a.
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Figure S32. Crystal packing structure of 4a.
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Figure S33. Photomicrographs of the HCT 116 cell lines before and after treatment with
complexes ; image control = HCT 116 cell before treatment, the images after treatment; A =
1a, B = 2a, C = 3a, D = 4a and 5-FU = standard reference.
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