Table S1. Sequences of primers designed for analyses of cpDNA sequence variation in Phleum
pratense
Name of primer
rps16
psbM
petN
trnQ-UUG
trnD-GUC
psbM
petN
trnC-GCA
psbH
petB
rpl16
rps3
petB
petD
trnCF nested
rpoB nested
rbcL nested
Primer sequence
gcacgttgctttctaccaca
cattttggctggctgttttt
cagcccaagcgagacttact
atcccgttactcggaggttc
ctgtcaaggcggaagctg
cattttggctggctgttttt
cagcccaagcgagacttact
caggggactgcaaatcctt
tcttctgttttactggacggaat
tgcaattgcctgaatctcaa
tgaaatgagaaagcgtgcaa
cttcgcattctgtccagtga
gtacctgacgccattccagt
ggctcgagcaagaatgaaag
cgagcgaaaatcgagaaaag
ccaggggtacaaatggaatg
gcggcttgtgaagtatggaa
Table S2. Summary of the output of the 11 SSR loci scored for variation in 19 populations of Phleum
pratense
SSR marker
A03A07
C02C08
C01B11
C02H01
A09H08
D01G10
A03E06
D01H08
A10A10
B03A09
D01E04
Total no. of Range of alleles
alleles
per individual
51
25
31
53
15
75
51
20
41
44
56
1-5
1-6
1-6
1-6
2-5
1-6
1-6
1-5
1-6
1-5
1-6
Average no.
of alleles per
individual
2.07
2.68
3.97
2.98
3.59
2.32
2.47
1.93
2.77
2.51
2.87
Table S3. Genetic diversity based on SSR marker analyses in 19 populations of Phleum pratense.
Accession number, country of origin and number of individuals, loci analysed and polymorphic loci is
given. The diversity indices calculated were mean number of pairwise differences between
individuals and average gene diversity over loci. Standard deviations are given.
Pop.
no
Accession
number
Country of
origin
No
No
of
of
inds. loci
11
10
12
15
14
8
19
40
46
44
45
38
39
16
27
34
25
1
3
NGB4053
NGB1332
NGB4140
NGB17198
NGB14403
NGB722
KEW51998
IHAR150183
IHAR150844
IHAR151366
GR4123
PL381926
PL406317
IHAR151908
PL210426
PL325461
PL204480
14G2400116
RCAT040682
Denmark
Sweden
Iceland
Norway
Finland
Sweden
England
Spain
Italy
Switzerland
Switzerland
France
Russia
Germany
Greece
Russia
Turkey
Czech Republic
Hungary
12
12
14
15
17
18
18
18
10
16
14
12
13
10
13
16
17
18
12
457
461
461
461
459
461
461
461
461
461
461
461
461
459
461
457
461
459
461
No
of
pol
loci
110
147
165
155
167
102
130
144
77
105
61
102
127
111
118
142
139
164
122
Average
differences
Average
gene
diversity
37.1 ± 17.38
43.7 ± 20.44
44.9 ± 20.72
41.6 ± 19.13
42.0 ± 19.17
30.3 ± 13.9
36.6 ± 16.71
38.8 ± 17.71
26.3 ± 12.6
31.0 ± 14.29
18.6 ± 8.79
36.1 ± 16.91
39.9 ± 18.57
37.0 ± 17.61
38.4 ± 18.85
39.0 ± 17.88
37.5 ± 17.15
41.1 ± 18.73
40.1 ± 18.77
0.08 ± 0.043
0.09 ± 0.050
0.10 ± 0.050
0.09 ± 0.047
0.09 ± 0.047
0.07 ± 0.034
0.08 ± 0.041
0.08 ± 0.043
0.06 ± 0.031
0.07 ± 0.035
0.04 ± 0.021
0.08 ± 0.041
0.09 ± 0.045
0.08 ± 0.043
0.08 ± 0.044
0.09 ± 0.044
0.08 ± 0.042
0.09 ± 0.046
0.09 ± 0.046