Program Director/Principal Investigator (Cannon , Judy, Lin):
BIOGRAPHICAL SKETCH
Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Judy L. Cannon
Assistant Professor
eRA COMMONS USER NAME (credential, e.g., agency login)
jcanno
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and
residency training if applicable.)
DEGREE
INSTITUTION AND LOCATION
MM/YY
FIELD OF STUDY
(if applicable)
University of California at Berkeley
San Francisco State University
The University of Chicago
The University of Chicago
B.A.
M.S.
Ph.D.
Postdoctoral
1995
1997
2003
2007
Biology
Cell Biology
Immunology
Immunology
Please refer to the application instructions in order to complete sections A, B, C, and D of the Biographical
Sketch.
A. Personal Statement
The goal of the proposed project is to study how the signaling molecule PKCθ drives T cell migration
downstream of CCR7. My training in immunology has focused on the understanding of how signaling networks
function together to control T cell activation and migration. My predoctoral training in the laboratory of Dr. Janis
Burkhardt used fluorescence microscopy and mutational analysis to identify the domains within the WiskottAldrich Syndrome Protein that regulated its localization to the immunological synapse. I then joined the
laboratory of Dr. Anne Sperling to study the role of the molecule CD43 in regulating T cell function. In Dr.
Sperling’s lab, I helped to identify a novel function for CD43 in regulating T cell migration. In work just accepted
for publication in Molecular Biology of the Cell, I also showed the molecular requirements for CD43 regulation
of T cell migration, including its interaction with the ezrin-radixin-moesin family of proteins. Since establishing
my own laboratory as a newly appointed assistant professor at the University of New Mexico School of
Medicine in March 2010, I have focused my work on identifying novel signaling regulators of T cell migration. I
recently obtained evidence showing a novel role for PKCθ in controlling T cell migration, and will use the
expertise gained in both my graduate and postdoctoral training to ascertain the precise mechanism by which
PKCθ regulates T cell migration. My training combines all the tools I will need to accomplish the aims set out in
my proposal. Under Dr. Burkhardt’s mentorship, I developed expertise in fluorescence microscopy to study the
actin cytoskeleton and protein localization. In my postdoctoral training in Dr. Sperling’s lab, I used in vivo
mouse models and flow cytometry to study T cell migration. I have extensive experience using structurefunction analyses to study molecular regulation of T cell activation and migration. In addition, I have now
assembled a strong group of collaborators and consultants to help me accomplish my research aims in new
areas of interest for my laboratory. The University of New Mexico Health Sciences Center is a NCI designated
Cancer Center and I have developed working collaborations with two leukemia experts to assist me in
developing an animal model to study T-ALL (Drs. Bridget Wilson and Stuart Winter). To study the role of
CARMA1 and the NF-κB pathway, I have taken advantage of both research expertise and reagents from Dr.
Marisa Alegre at the University of Chicago who specializes in the study of CARMA1 and NF-κB in T cell
function. Dr. Jordan Jacobelli and Rachel Friedman, recently appointed new Assistant Professors at the
National Jewish Health Center and University of Colorado have agreed to provide technical advice and
assistance for multiphoton microscopy and intravital imaging. My own training and my team of consultants and
collaborators put me in a strong position to use the cutting edge fluorescence expertise and technology
available to me at the University of New Mexico to accomplish the aims set out in this proposal.
PHS 398/2590 (Rev. 06/09)
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Biographical Sketch Format Page
Program Director/Principal Investigator (Cannon , Judy, Lin):
B. Positions and Honors
Positions and Employment
1995
Lawrence Berkeley Laboratories, Human Genome Project, Technician, Berkeley, CA.
1995-1997
Master’s Degree candidate, Cell Biology, Department of Biology, SFSU, SF, CA.
1997-2003
Predoctoral Fellow, Committee on Immunology, Univ. of Chicago, Chicago, IL.
2003-2008
Postdoctoral Fellow, Dept. of Med Pulmonary and Critical Care Med, Univ. of Chicago.
2008-2010
Research Associate (Assistant Professor), Department of Medicine, Section of
Pulmonary & Critical Care, The University of Chicago, Chicago, IL.
2010-present
Assistant Professor, Depts of Molecular Genetics and Microbiology and Pathology, Univ
of New Mexico Health Sciences Center, Albuquerque, NM.
Other Experience and Professional Memberships
2007 – present
American Association of Immunologists
Honors
1991-1995
1995-1997
1997-2000
2003-2005
2005-2008
2008
Chancellor’s Scholarship, University of California at Berkeley, Berkeley, CA.
GAANN Fellowship, San Francisco State University, San Francisco, CA.
Searle Fellowship, University of Chicago, Chicago, IL.
NIH Post-doctoral Fellowship, University of Chicago, Chicago, IL.
Cancer Research Institute Post-doctoral Fellowship.
Huggins Lectureship, University of Chicago, Chicago, IL.
C. Selected Peer-reviewed Publications
Most relevant to the current application
1. Cannon, J.L.#, Mody, P.D., Blaine, K.M., Nelson, A.D., Sayles, L., Moore, T.V., Clay, B.S., Bandukwala,
H.S., Dulin, N.O., Shilling, R.A., Sperling, A.I. CD43 interaction with ERM proteins regulates T cell
trafficking and CD43 phosphorylation. Molecular Biology of the Cell, in press. #corresponding author.
2. Mody, P.D.*, Cannon, J.L.*, Bandukwala, H.S., Blaine, K.M., Schilling, A.B., Swier K., Sperling, A.I. 2007.
Signaling through CD43 is required for CD4 T cell trafficking. Blood, Oct 15;110(8):2974-82. *co-first
authorship. PMID: 17638845.
3. Cannon, J.L., Burkhardt, J.K. Differential roles for WASP in actin regulation and IL2 production. 2004.
Journal of Immunology 173(3):1658-62.
4. Zeng, R., Cannon, J.L., Abraham, R.T., Way, M., Billadeau, D.D., Bubeck-Wardenberg, J., Burkhardt, J.K.
2003. SLP-76 coordinates Nck-dependent Wiskott-Aldrich syndrome protein recruitment with Vav-1/Cdc42dependent Wiskott-Aldrich syndrome protein activation at the T cell-APC contact site. Journal of
Immunology 171(3):1360-8. PMID: 12874226.
5. Cannon, J.L., C. Case, G. Bosco, A. Seth, M. McGavin, K.A. Siminovitch, M.K. Rosen, and J.K. Burkhardt.
2001. WASP recruitment to the T cell-APC contact site occurs independently of Cdc42 Activation.
Immunity 15:249-259. PMID: 11520460.
Additional recent publications
6. Sedwick, C.E., M.M. Morgan, L. Jusino, J.L. Cannon, J. Miller and J.K. Burkhardt. 1999. TCR, LFA-1 and
CD28 play unique and complementary roles in T cell cytoskeletal reorganization. Journal of Immunology
162:1367-1375. PMID: 9973391.
7. Allenspach, E.J., P. Cullinan, J. Tong, Q. Tang, A.G. Tesciuba, J.L. Cannon, S.M. Takahashi, R. Morgan,
J.K. Burkhardt, and A.I. Sperling. 2001. ERM-dependent movement of CD43 defines a novel protein complex
distal to the immunological synapse. Immunity, 15:739-750. PMID: 11728336
PHS 398/2590 (Rev. 06/09)
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Program Director/Principal Investigator (Cannon , Judy, Lin):
8. Bandukwala, H.S., B.S. Clay, J. Tong, P.D. Mody, J.L. Cannon, R.A. Shilling, J.S. Verbeek, J.V. Weinstock,
J. Solway, and A.I. Sperling. 2006. Signaling through FcγRIII is required for optimal Th2 responses and Th2mediated airway inflammation. Journal of Experimental Medicine 204(8):1875-89. PMID: 17664287
9. Cannon, J.L. *#, Collins A. *, Balachandran D., Henriksen, K.J., Clay, B.S., Mody, P.D., Smith, C.E., Tong
J., Miller S.D., Sperling A.I. 2008. CD43 regulates Th2 differentiation and inflammation. Journal of Immunology,
180: 7385-7393. *co-first authorship, #corresponding author. PMID: 18490738
10. Shilling, R.A., Clay, B.S., Moore, T.V., Berry, E., Tesciuba, A.G., Bandukwala, H.S., Tong, J., Weinstock,
J.V., Flavell, R.A., Horan, T., Yoshinaga S.K., Welcher, A.A., Cannon, J.L., Sperling, A.I. 2009. CD28 and
ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo. Cellular
Immunology, 2009;259(2):177-84. PMID: 19646680
11. Clay BS, Shilling RA, Bandukwala HS, Moore TV, Cannon JL, Welcher AA, Weinstock JV, Sperling AI.
2009. Inducible costimulator expression regulates the magnitude of Th2-mediated airway inflammation by
regulating the number of Th2 cells. PLoS One. Nov 4;4(11):e7525. PMID: 19888475
12. Shaffer MH, Huang Y, Corbo E, Wu GF, Velez M, Choi JK, Saotome I, Cannon JL, McClatchey AI,
Sperling AI, Maltzman JS, Oliver PM, Bhandoola A, Laufer TM, Burkhardt JK. 2010. Ezrin is highly expressed
in early thymocytes, but dispensable for T cell development in mice. PLoS One. 2010 Aug 27;5(8). pii: e12404.
PMID: 20806059
13. Driessens, G., Zheng, Y., Locke, F., Cannon, J.L., Gounari, F., Gajewski, T.F. β-catenin inhibits T cell
activation by selective interference with LAT-PLC-γ1 phosphorylation. Journal of Immunology, in press. PMID:
21149602
14. Moore TV, Clay BS, Ferreira CM, Williams JW, Rogozinska M, Cannon JL, Shilling RA, Marzo AL,
Sperling AI. Protective effector memory CD4 T cells depend on ICOS for survival.
PLoS One. 2011 Feb 18;6(2):e16529. PMID: 2136474
15. Moore TV, Clay BS, Cannon JL, Histed A, Shilling RA, Sperling AI. ICOS Controls T Cell Migration to the
Lungs via Down-Regulation of CCR7 and CD62L. Am J Respir Cell Mol Biol. 2011. Vol. 45, pp. 843-850, 2011.
PMID: 21421907
D. Research Support
Ongoing Research Support
Scientist Development Grant
Cannon (PI)
7/1/09-6/30/13
American Heart Association
PKCθ phosphorylation of CD43 via ERM proteins regulates T cell trafficking
Study the molecular regulation of T cell trafficking by PKCθ, ERM proteins, and CD43 and define the
mechanism of molecular interaction between PKCθ, ERM proteins, and CD43.
Role: PI
R01 AI097202-01A1
Cannon (PI)
5/1/12-4/30/17
The role of PKCtheta in T cell and T-ALL migration
The goal of this application is to elucidate the mechanisms involved in controlling T cell migration and migration
of T-ALL leukemia cells.
Role: PI
1R21HL106462
NIH/NHLBI
PHS 398/2590 (Rev. 06/09)
Corey, Seth (PI)
Page
10/01/11-06/30/13
Biographical Sketch Format Page
Program Director/Principal Investigator (Cannon , Judy, Lin):
The F-BAR protein CIP4 in WASP dependent thrombocytopenia
The goal of this project is to model T cells searching for antigen in lymph nodes.
Role: Subaward
Completed Research Support
Illinois Division Research Grant
Cannon (PI)
12/01/08-11/30/09
American Cancer Society-IL. Division
PKCθ phosphorylation of CD43 via ERM proteins regulates T cell trafficking
The goal of this project is to delineate the signaling pathways that lead to CD43 phosphorylation and normal T
cell trafficking to lymph nodes, specifically, the roles of ERM proteins and PKCθ.
Role: PI
PHS 398/2590 (Rev. 06/09)
Page
Biographical Sketch Format Page