1 Evaluation of Factor Structure and Validity of HIV Knowledge Questionnaire By Talwar P1, Ghazalia SR 2, Ahmad R3 & Mohd Fadzil AR4 Dr. Prashanth Talwar1Ph.D Associate Professor Universiti Malaysia Sarawak 94300 Kota Samrahan. Dr. Siti Raudzah Ghazalia 2Ph.D Associate Professor Universiti Malaysia Sarawak 94300 Kota Samrahan. Dr. Rusli Ahmad 3 Ph.D Dean (PPP) and Associate Professor Universiti Malaysia Sarawak 94300 Kota Samrahan. Prof. Mohd Fadzil Abdul Rahman4MA Deputy Vice Chancellor and Professor Universiti Malaysia Sarawak 94300 Kota Samrahan. Corresponding Author Dr. Prashanth Talwar1Ph.D Associate Professor Universiti Malaysia Sarawak 94300 Kota Samrahan. drpytalwar@yahoo.com Statistical summary of the manuscript Total Number of words: 3707. Number of words in abstract: 155 Number of references: 25 Number of Tables: 2 2 Evaluation of Factor Structure and Validity of HIV Knowledge Questionnaire Abstract Background: HIV is a major social problem which can be prevent, unfortunately many young adults lack comprehensive and adequate knowledge. There are many scales available to measure knowledge on HIV. The brief HIV Knowledge Questionnaire (HIV-KQ-18) was designed to provide a more concise and well-established measure of HIV-related knowledge. The objective of this study is to assess the factor structure and internal consistency of the HIV-KQ-18 among university students in Malaysia. Method: The study was conducted in one of the Universities in Malaysia. Questionnaires and the HIV-KQ-18 scale was distributed in the class room and taken back on completion. 405 students completed the questionnaires and the scale in all respects. Results: Factor analysis explained 58.35 % of the variance and items clustered together into subscales. The internal consistency was 0.60. Conclusion: The HIV-KQ-18 scale was easy to understand with 71% correct answers. The internal consistency of the scale is considered under lower limit of acceptability. Key Words: Knowledge. HIV Scale. Factor Analysis. Internal consistency. 3 Factor Analysis of HIV-KQ-18 among a group of Malaysian University Students Introduction HIV/AIDS is a major public health problem. Every day, more than 10 Malaysians are tested positive for HIV infection [1] . The continuous increase in number of people living with HIV/AIDS (PLWHA) represents a serious health and economic burden [2] . The increasing trend of premarital sexual experience and unintended pregnancies in Malaysia warrants sustained and serious attention [3] . HIV is a stigmatizing medical condition. The concept of HIV stigma is multifaceted, with personalized stigma (perceived stigmatizing consequences of others knowing of their HIV status), disclosure concerns, negative self-image, and concerns with public attitudes described as core aspects of stigma for individuals with HIV infection [4]. Knowledge is a crucial factor of HIV risk reduction. Many teenagers are knowledgeable about the risks and consequences of HIV, yet a large percentage do not perceive that they are personally at risk. Gaining insight into the perceptions and factors influencing the behavior of teens is critical in HIV and AIDS prevention [5] . The stigma of and discrimination because of HIV has been described as the most important obstacle to prevention and treatment efforts Bandura [7] [6] . emphasized that prevention of infection with the AIDS virus requires people to exercise influence over their own behavior and their social environment. Societal efforts designed to control the spread of AIDS have centered mainly on informing the public on how the human immunodeficiency virus (HIV) is transmitted and how to safeguard against such infection. It is widely assumed that if people are adequately informed about the AIDS threat they will take appropriate self-protective action. Bandura [7] maintains that heightened awareness and knowledge of health risks are important preconditions for self-directed change. Unfortunately, information alone does not necessarily exert much influence on refractory health-impairing 4 habits. To achieve self-directed change, people need to be given not only reasons to alter risky habits but also the behavioral means, resources, and social supports to do so. Effective selfregulation of behavior is not achieved by an act of will. It requires certain skills in selfmotivation and self-guidance. In the context of the evaluation of prevention programs, it is necessary to have valid, reliable and parsimonious instruments to assess the degree of knowledge in the target population. Few questionnaires are specifically designed for adolescents. This means that the information content on HIV/AIDS is not necessarily the most appropriate for this population and the psychometric properties of the scale are not guaranteed for use with adolescents [8]. The HIV Knowledge Questionnaire originally developed by Carey and Schroder [9] consists of 45 items and known as HIV-KQ-45. Although the HIV-KQ-45 is psychometrically strong, it is impractical for street outreach, field surveys, and intervention evaluation work in settings where evaluators also wish to assess knowledge as well as other constructs with minimum respondent burden. Needed is a briefer, but psychometrically well-established measure [9] . The brief HIV Knowledge Questionnaire (HIV-KQ-18) was designed to provide a more concise and wellestablished measure of HIV-related knowledge in street outreach, fieldwork, and intervention settings. The scale aims to tap into other related constructs, for which the original HIV-KQ 45item scale did not address [9]. One of the factors that influence risk behavior among students is their HIV/AIDS knowledge. The objective of this study is to assess the factor structure and internal consistency of the HIVKQ-18 among university students in Malaysia. 5 MATERIALS AND METHODS Participants: The study was conducted in one of the Universities in Malaysia. The undergraduate students who volunteered to participate in the study were explained about the purpose of the study and implications. The students were assured anonymity and were told to respond honestly. Study was approved by university research committee. Sample: Questionnaires and the scale was distributed in the class room and taken back on completion. 405 students completed the questionnaires and the scale in all respects. Instrumentation: The HIV Knowledge Questionnaire (HIV-KQ-18) has 18-items. It is a brief self-administered measure of the individual's HIV-related knowledge. This instrument contains 18 forced-choice statements ('true', 'false', 'don't know') knowledge related to sexual transmission. A single summary score is yielded overall, with higher scores significant of greater HIV-related knowledge. ‘Don’t know’ response are scored incorrect. Analysis SPSS version 21 was used to analyze the data. Kaiser-Meyer-Olkin (KMO) measure of sampling adequacy suggests that pattern of correlation are relatively compact and so factor analysis should yield distinct and reliable factors. KMO value is 0.617, which is considered mediocre. Bartlett’s test of sphericity was 2=629.47 (df=153, p<0.000). In this study, the sample inter correlation matrix did not come from a population in which the inter correlation matrix is an identical matrix. There was no correlation error among the variables. 6 Exploratory factor analysis, with varimax rotation, was applied to the data. Eigen values above 1.00 were used. The criterion for factor loading was set at ≥ 0.40 to suppress absolute value less than 0.40. Principle component analysis was used as extraction method. Factor loadings and screen plot were examined. One good method of screening for efficient items is to run an exploratory factor analysis on all the items to eliminate those variables that failed to show high correlation [10]. Cronbach’s alpha [11] was used to find the internal consistency of the scale. Cronbach's alpha is an index of reliability associated with the variation accounted for by the true score of the "underlying construct." Construct is the hypothetical variable that is being measured [12] . The higher the score, the more reliable the generated scale is. Results The mean age of students was 21 years. 42% were male students while 58% were female students. 2% were Hindus’, 4% Buddhists’, 33% Christians’ and 61% Muslims’. 42% were male students while 58% were female students. Percentage of correct answer. As seen in Table No. 1, the mean and standard deviation varied from individual items. Frequency of correct answers differed from one item to another. The total mean score was 12.81. (71 % correct ). 7 Table No. 1 HIV-KQ-18: Individual item mean and standard deviation ITEM 1 Mean Coughing and sneezing DO .74 SD .43 NOT spread HIV 2 A person can get HIV by .81 .38 sharing a glass of water with someone who has HIV 3 Pulling out the penis before a .69 .46 man climaxes/cums keeps a woman from getting HIV during sex 4 A woman can get HIV if she .79 .40 has anal sex with a man 5 Showering, or washing one’s .81 .38 genitals/private parts, after sex keeps a person from getting HIV 6 All pregnant women infected .21 with HIV will have babies born with AIDS .41 8 7 People who have been .81 .38 infected with HIV quickly show serious signs of being infected 8 There is a vaccine that can .67 .46 stop adults from getting HIV 9 People are likely to get HIV .72 .44 by deep kissing, putting their tongue in their partner’s mouth, if their partner has HIV 10 A woman cannot get HIV if .83 .37 she has sex during her period 11 There is a female condom .61 .48 that can help decrease a woman’s chance of getting HIV 12 A natural skin condom .67 works better against HIV than does a latex condom .46 9 13 A person will NOT get HIV .71 .45 if she or he is taking antibiotics 14 Having sex with more than .94 .22 one partner can increase a person’s chance of being infected with HIV 15 Taking a test for HIV one .56 .49 week after having sex will tell a person if she or he has HIV 16 A person can get HIV by .84 .36 sitting in a hot tub or a swimming pool with a person who has HIV 17 A person can get HIV from .56 .49 oral sex 18 Using Vaseline or baby oil .84 with condoms lowers the chance of getting HIV Total Score (71 % correct) 12.81 .36 10 Factor Structure of HIV KQ-18 The overall results from the Exploratory Factor Analysis are presented in Table 2. The HIV-KQ18 was factor analyzed; Principal Component Analysis was used to explore factor structures. The seven factor solution accounted for 55.79 % of the total variance. Item No. 4 failed to load. Factor analysis was redone by eliminating item No. 4, the total variance increased to 58.35 %. It was found that the loadings ranged from 0.43 to 0.83. Majority of the loading was >0.40. Only one item (7) showed poor loading (0.432). Varimax rotation was applied resulting in 7 subscales solutions. The first factor accounted for 13.67 % of the variance and included four items 6,7,12 & 13. The second factor accounted for 10.21 % of the variance and included three items 9, 16 & 17. The third factor accounted for 8.00 % of the variance and included two items 3 & 5. The fourth factor accounted for 7.28 % of the variance and included two items 1&18 The fifth factor accounted for 6.74 % of the variance and included two items representing items 2 &11. The sixth factor accounted for 6.46 % of the variance and included two items 10 & 14. The seventh factor accounted for 5.99 % of the variance and included two items 8 &15. Internal consistency Cronbach’s α was 0.40. If items with low homogeneity index (HI) were to be deleted it is presumed that there would be substantial increase in alpha. Item No. 4,6,11,15 & 17 were deleted and it was found that the Cronbach’s α increased substantially to 0.60. 11 Discussion HIV/AIDS knowledge is an important component of HIV/AIDS risk prevention strategies that may influence engagement in high risk behavior [13] . One property that may drive this inference process is the degree to which the content of knowledge on which the attitude is based is directly relevant to the goal of the behavior [14]. Because knowledge is a key component of HIV risk reduction programs, interventions often use knowledge assessment guide educational curricula and to provide feedback to enhance risk awareness [15]. The relationship between risk knowledge, attitude, and behavior among different population groups is complex and has not been sufficiently explored [16]. HIV scales are useful to determine the effectiveness of education provided to different groups. There are many scales to measure HIV knowledge, 11 item HIV risk-taking behavior scale (HRBS) [17] , the 14-item human immunodeficiency virus/acquired immunodeficiency syndrome knowledge scale [18] , the perceived risk of HIV scale (PRHS) [19] and the HIV/AIDS Knowledge and Attitudes Scales for Teachers [20]. The HIV knowledge questionnaire [21] , is a self –administered instrument. It consisted of 45 items. The 45 item scale was reduced to 18 item scale based on empirical item analysis, called as the HIV-KQ-18 scale. It is easy to understand at 7th grade level and can be used across cultures. A single summary score is obtained by summing the number of items correctly answered. Carry reported correlation that ranged from .24 to .57. and found that the internal consistency ranged from .75 to .89 across samples. 12 The objective of this study is to assess the factor structure internal consistency and of the HIVKQ-18 among university students in Malaysia. It appears that there was no difficulty in understanding the content of the HIV-KQ-18 scale among Malaysian students. Only one item ie “All pregnant women infected with HIV will have babies born with AIDS” had a low percentage of correct answer (22%). This is consistent with a similar study done by Swenson et al [22] among African American Adolescents, who reported 85 % of the students thought that all pregnant women infected with HIV will have babies born with AIDS. Overall, 71 % correct answers on an 18 item scale in this study is reasonable good enough. Factor analysis was done to see whether items clustered together into subscales. Results of this study found that the HIV-KQ-18 had seven factors which explained 58.35 % of the variance. Factor I explained 13.67 % variance. Factor II explained 10.21 % variance. Factor III explained 8.00 % variance. Factor IV explained 7.28 % variance. Factor V explained 6.74 % variance. Factor VI explained 6.46 % variance. Last Factor 7 explained 5.99 % variance. Cronbach’s alpha is generally used as a measure of the reliability of a set of questions in a survey instrument. It measures the interrelatedness of a set of items, although a high value for alpha does not imply uni-dimensionality (where the items measure a single latent construct) Consider the typical assessment by a researcher who achieves an alpha that [10] . exceeds .70 and concludes, based on Nunnally’s[23] rule-of-thumb, that the level of reliability is “sufficient”. What Nunnally[23] actually said is that “in the early stages of predictive or construct validation research,” it may be “satisfactory” to “have only modest reliability. For other scenarios, Nunnally [23] goes on to state that .80 or even .90 may be required. Further, if one computes an 13 alpha equal to .70, with a confidence interval that ranges from .60 to .80, it is not so obvious that an “acceptable level of reliability” has been achieved [11]. Carry [9] reported that the stability coefficients varied between .75 to .89, providing evidence for satisfactory to excellent reliability of the test scores. In this study the internal consistency was 0.60 which is considered lower limit of acceptability. The reason why Cronbach's alpha was low could be because of the number of items in the scale. If the items in the scale is too short, the value of alpha is reduced [24] . Increasing the number of items can increase the value of alpha to an acceptable level. This reveals that the scales with a greater number of items are more reliable. In this study the random error was 0.64. It is a well-known fact that random error can affect the degree of reliability. Where there is very little random error, the measure is reliable [25]. Conclusion and Future research. The findings of this study may be interpreted in with its limitations. This study was conducted on a homogeneous sample of undergraduate student from only one university and the small sample size was substantially small. The HIV-KQ-18 scale was easy to understand with 71% correct answers. Factor analysis showed items clustered together into subscales. The internal consistency was 0.60 which is considered lower limit of acceptability. Replication of this study need to be done among different group of population to test the internal consistency of the HIV-KQ-18 scale. 14 TABLE No.2 Exploratory Factor Analysis [Malay version of the HIV-KQ-18 with English translation] ITEM 1 F1 F2 F3 Coughing and sneezing DO F4 F5 .768 NOT spread HIV 2 A person can get HIV by .568 sharing a glass of water with someone who has HIV 3 Pulling out the penis before a 0.784 man climaxes/cums keeps a woman from getting HIV during sex 4 A woman can get HIV if she has anal sex with a man 5 Showering, or washing one’s genitals/private parts, after sex keeps a person from getting HIV 6 All pregnant women infected .698 with HIV will have babies born with AIDS 0.565 F6 F7 15 7 People who have been .432 infected with HIV quickly show serious signs of being infected 8 There is a vaccine that can .510 0.525 stop adults from getting HIV 9 People are likely to get HIV 0.717 by deep kissing, putting their tongue in their partner’s mouth, if their partner has HIV 10 A woman cannot get HIV if 0.451 she has sex during her period 11 There is a female condom .413 .661 that can help decrease a woman’s chance of getting HIV 12 A natural skin condom .433 works better against HIV than does a latex condom .529 16 13 A person will NOT get HIV .629 if she or he is taking antibiotics 14 Having sex with more than .832 one partner can increase a person’s chance of being infected with HIV 15 Taking a test for HIV one .810 week after having sex will tell a person if she or he has HIV 16 A person can get HIV by 0.602 sitting in a hot tub or a swimming pool with a person who has HIV 17 A person can get HIV from 0.603 oral sex 18 Using Vaseline or baby oil with condoms lowers the chance of getting HIV 0.614 17 REFERENCES 1. 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