1 2 Table S2: Description of prey categories used in field observations for 2009 MSI puffin chick diet and corresponding DNA taxa. 2009 Diet Method Comparison Field Observation Prey Category Butterfish Observed 1995-2010 (# Items) 350 Krill 5121 Hake 4580 + 270 (larval) Urophycis tenuis, U. chuss, and Enchelyopus cimbrius Urophycis tenuis, Urophycis sp., and Enchelyopus cimbrius Hake or Herring 1304 Fish belonging to ‘Hake’ group or Clupea harengus N/A Field-Observed Prey Category Description Peprilus triacanthus Any krill (Euphausiacea) but typically Meganyctiphanes norvegica DNA-Identified Taxa Peprilus triacanthus Meganyctiphanes norvegica, Thysanoessa sp. Clupea harengus Clupea harengus Pollock 6328 + 126 (larval) 63 Pollachius virens Polychaete 132 Any Polychaete (Polychaeta) Not detected Nereis pelagica, Hediste diversicolor, Polychaeta Sandlance 2759 + 222 (larval) Sculpin 5 Squid Unidentified Fish Unidentified Rock Eel/Gunnel Bluefish Crustacean Lumpfish Stickleback Atlantic Saury Fish Scrap Eelpout Earthworm N/A N/A N/A N/A N/A N/A 85 3400 + 2480 (larval) 723 Herring Typically Ammodytes americanus, potentially A. dubius Fish that look like sculpin (Cottoidea) Any squid Myoxocephalus aenaeus, Hemitripterus americanus Not detected Any unknown fish N/A Any unknown item N/A 1 Not detected Pholis gunnellus 2 3 3 3 4 17 36 42 N/A N/A N/A N/A N/A N/A Not detected Not detected Not detected Not detected Not detected Not detected Not detected Not detected Not detected Ammodytes sp Pomatomus saltatrix Multiple species Not detected Not detected Not detected N/A Not detected Not detected Hippolytidae Melanogrammus aeglefinus Liparis inquilinus Gaidropsarus ensis Sebastes fasciatus Cryptacanthodes giganteus 2009 Diet Method Comparison 3 4 5 6 Field Observed Field-Observed Prey Observation 1995-2010 (# DNA-Identified Taxa Category Description Prey Category Items) N/A N/A Not detected Gadus morhua N/A N/A Not detected Brachycera N/A N/A Not detected Neoptera Number of prey items identified from field observations of provisioning adults on Machias Seal Island 1995-2010 (LEFT, [23]). Description of prey categories identified in 2009 field observations and the associated DNA-identified taxa (RIGHT). Bolded prey: observed with one method only.