Christopher Alan Hack
1069 El Centro Avenue, Apt. 2
Oakland, CA 94602
(510)-703-2522
umphack@gmail.com
EDUCATION:
B.S., Chemistry, 1996, University of California at Berkeley
EXPERIENCE:
May ’12 – Mar. ’14: Field Systems Engineer for Biolytic Lab Performance, Inc. Built or
rebuilt instrumentation for DNA synthesis, peptide synthesis, and
oligonuclotide purification, including Dr. Oligo 192, ABI 3900,
Biolytic 3900, Expedite 8909, and ABI 394 DNA synthesizers, ABI
433 and Symphony peptide synthesizers, and Dr Oligo Processor. As
Field Service Engineer, installed instrumentation at customer sites,
trained customers on the instruments and supporting software,
performed periodic preventative maintenance, and diagnosed and
repaired hardware and software problems as necessary. R&D duties
included providing input to, testing, validating, and developing
protocols for the use of novel instrumentation and software. Duties
also included writing user manuals, technical notes, training
materials, and assembly instructions to accompany products.
Feb. ’08 – May ‘12: Senior Research Associate in the Production Process Optimiazation
Group at the Department of Energy (DOE) Joint Genome Institute.
Initiated design of process to automate the selective enrichment of
DNA sample-containing capture beads for Roche/454 DNA
sequencing. Designed, programmed, and optimized process to
construct single- and double-stranded DNA libraries for sequencing
by the Roche/454 and Illumina/Solexa methods. Wrote standard
operating procedures and implemented developments into the DNA
sequencing production line. Programmed and maintained optimal
performance on Beckman-Coulter liquid handling robots, Covaris
ultrasonicators, and Caliper electrophoresis instruments. Supported
the Microscale Applications (Single-cell genomics) pipeline with
reagent QC, maintenance of instruments (Roche light-cycler and
Cytopeia flow cytometer), and optimization of lysis protocols.
Received SPOT award for work done as part of the Safety Culture
Working Group.
Nov. ’01 – Jan. ‘08: Senior Research Associate in the Proteomics and Genomic
Technolgy groups at Joint Genome Institute. Designed and
implemented experiments to characterize protein-DNA interactions of
transcription factors using Biomolecular Interaction Analysis (BIA)
and MALDI-TOF mass spectrometry. Designed single-stranded
DNA binding targets for transcription factors using Systemic
Enrichment of Ligands by EXponential enrichment (SELEX).
Optimized and implemented protocols for purifying Histidine-tagged
proteins using Nickel-charged resins. Optimized DNA sequencing
protocols for use with both Amersham MegaBace and ABI sequncers.
Programmed and maintained optimal performance on BeckmanCoulter liquid handling robots.
Oct. ’96 – Oct. ‘01:Research technician in a mass spectrometry group at Lawrence
Berkeley National Laboratory (LBNL). Performed data collection
and analysis on MALDI-TOF and electrospray ionization mass
spectrometers. Performed molecular biological and biochemical
procedures such as cloning, sequencing, PCR, agarose gel
electrophoresis, and gel extraction to prepare protein and DNA
samples for mass spectrometry analysis. Designed experiments from
scratch and participated in preparing results for publication. Received
Outstanding Performance Award for experimental work on Ligase
Chain Reaction (LCR) and Peptide Nucleic Acid (PNA) probes.
Feb ’98 – Present: Baseball umpire. Officiated baseball games at high school, college,
and semi-professional levels. As an administrator, was responsible
for assigning officials to umpire high school games, keeping detailed
game and payment records, maintaining a training and eligibility
database, and troubleshooting scheduling conflicts as they arose. As
an instructor, led training clinics for new and advanced umpires.
SKILLS:
Field Service – interaction with customers, product installation, customer training,
preventative maintenance, diagnosis and repair of malfunctions.
DNA Synthesis on a variety of platforms, including Dr. Oligo 192, ABI 3900,
Expedite 8909, and ABI 394.
Analytical chemistry techniques, with extensive experience on TOF mass
spectrometry systems.
Molecular biology and biochemistry techniques, including protein purification,
PCR, cloning, sequencing, and gel electrophoresis.
Biomolecular Interaction Analysis on BIAcore instruments.
Liquid-handling robot programming and maintenance (Beckman-Coulter Biomek)
Agilent 2100 Lab-on-a-chip Bioanalyzer
Caliper high-throughput electrophoresis
Sample ultrasonication using Covaris technology
Micromanipulation of single cells using Zeiss microscope equipment.
Flow cytometry and single-cell sorting with Cytopeia flow cytometer
qPCR, real-time Multidisplacement Amplification (rtMDA), and other
applications of Roche light-cycler
Technical Communication skills – writing User Manuals, Technical Notes, and
Training Materials.
Word-processing (e.g. Microsoft Word).
Spreadsheet programming (e.g. Excel).
Electronic Presentation programming (e.g. PowerPoint).
Project management skills, including multi-task time management, inventory
maintenance, procurement
Teaching/training skills, both in group and individual settings
OTHER ACTIVITIES:
Alpha Chi Sigma Professional Chemistry Fraternity: Participated in activities to
promote chemistry to various student groups. Activities included tutoring,
performing chemistry demonstrations at schools, and tributes to famous
chemists at public events.
PUBLICATIONS:
C. Hack, J. Chang, Illumina Unamplified Indexed Library Construction: An
Automated Approach, 6th Annual U.S. DOE Joint Genome Institute User
Meeting, March 2011.
S. Deshpande, C. Hack, J. Chang, et al., Development of High Throughput
Process for Constructing 454 Titanium Libraries, 5th Annual U.S. DOE Joint
Genome Institute User Meeting, March, 2010.
E. Tang, C. Hack, J. Chang, et al., Development of High Throughput Process for
Constructing Illumina Libraries, 5th Annual U.S. DOE Joint Genome Institute
User Meeting, March, 2010.
C. Daum, C. Hack, S. Lucas, et al., Optimization of the 454 Production
Sequencing Workflow at the DOE Joint Genome Institute, 454 Users
Conference, September, 2008.
C. Daum, C. Hack, S. Lucas, et al., DOE Joint Genome Institute, 9th Annual
Advances in Genome Biology and Technology Meeting, February, 2008.
C. Hack, P. Richardson, et al., The Pyrosequencing Process, 2nd Annual U.S.
DOE Joint Genome Institute User Meeting, March, 2007.
C. Hack, E. Rubin, et al., New Methods to Characterize Transcription Factors
and their Cis-regulatory Binding Regions using BIAcore-based Ligand
Fishing, DOE New Horizons in Genomics Workshop, March, 2003.
C. Hack, P. Richardson, et al., Ligand Fishing with Biomolecular Interaction
Analysis (BIA) and MALDI-TOF MS, 50th ASMS Conference on Mass
Spectrometry and Allied Topics, June, 2002.
C. Hack, W. Benner, A Simple Algorithm Improves Mass Accuracy to 50-100 ppm
for Linear Delayed Extraction MALDI-TOF MS Measurements, Rapid
Communications in Mass Spectrometry, 2002, V16(13): 1304-1312.
J. Ullom, C. Hack, W. Benner, et al., Discrimination Between Bacterial Spore
Types Using Time-of-Flight Mass Spectrometry and Matrix-Free Infrared
Desorption and Ionization, Analytical Chemistry, 2001, V73: 2331-2337.
J. Schultz, C. Hack, W. Benner, Polymerase chain reaction products analyzed by
charge detection mass spectrometry, Rapid Communications in Mass
Spectrometry, 1999, V13(1): 15-20.
F. Zhong, C. Hack, W. Benner, et al., DNA Typing with Peptide Nucleic Acid
(PNA) Probes and Delayed Extraction MALDI-TOF Mass Spectrometry, 46th
ASMS Conference on Mass Spectrometry and Allied Topics, June, 1998.
J. Schultz, C. Hack, W. Benner, Mass determination of megadalton-DNA
electrospray ions using charge detection mass spectrometry, Journal of the
American Society for Mass Spectrometry, April, 1998, V9(4): 305-313.
REFERENCES:
Available upon request