Table 1. Sequences of the primers used in this study. Primer
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Table 1. Sequences of the primers used in this study. Primer Sequence (5′→3′) Description 3P1 3P2 TGCTTCCGGTGGTTCTATCTG GCAGGTGCAGACCATACTCGA Gene-specific primer for 3’RACE Nested gene-specific primer for 3’RACE 3Tras CGCAGTCGGTAC(T)12AGC The adapter primer 3Prox The universal outer adaptor primer ORFF ACGTGGACTATCCATGAACGCAC GCAGTCGGTAC(T)13 TCGAGCGGCCGCCCGGGCAG GTCGACGTGGACTATCCATGAACGCA ATGGAAGGGGATACTTTCTCAGG ORFR CTACCCAGATCTAACTCTCTTAAGACCA Reverse primer for ORF PCR RTPF GGGCAGGTGCAGACCATAC Forward primer for real-time quantitat ive RT-PCR RTPR CGTTGCGGCTGAGGTTGT Reverse primer for real-time quantitat ive RT-PCR ACT2F TCCCAAGGCAAACAGAGAAAAG Actin primer as Doritaenopsis internal control, forward ACT2R GCCACTAGCATATAGGGAAAGAAC Actin primer as Doritaenopsis internal control, reverse FLF TGTGCTTCCGGTGGTTCTATC FLR CATTGAAGAGATGAATAAGAACTGC Ud ix The universal nested adaptor primer Forward primer for ORF PCR Forward specific primer for DhEFL4 cDNA primer for DhEFL4 cDNA sequence (containing ORF) Reverse 1AP gene gene specific sequence (containing ORF) The adaptor 2AP GTAATACGACTCACTATAGGGCACGCG TGGTCGACGGCCCGGGCTGGT PO4-ACCAGCCC- NH2 AP1 GTAATACGACTCACTATAGGGC The universal outer adaptor primer AP2 ACTATAGGGCACGCGTGGT The universal nested adaptor primer 1GOR ATGGTAATCTATGGCAGCAATG 1GIR TGTATATCAAATTCCAATGCACTCTT 2GOR TTTACTTGGCTTCCGAGTTGAT 2GIR TAATGGGTGGGAGGGTCCAGAT 3GOR CATTTATGCGTCATCAAGAA 3GIR ATTAAAAAATTATCTAAATCTCTCA WF1 AAGATGATTTCCCACTCACTCGTT WF2 TGTGCTTCCGGTGGTTCTATC Another adaptor Outer gene specific primer for DhEFL4 first genome walking Nested gene specific primer for DhEFL4 first genome walking Outer gene specific primer for DhEFL4 second genome walking Nested gene specific primer for DhEFL4 second genome walking Outer gene specific primer for DhEFL4 third genome walking Nested gene specific primer for DhEFL4 third genome walking Forward gene specific primer for DhEFL4 for DhEFL4 for DhEFL4 whole promoter sequence (Outer) Forward gene specific primer whole promoter sequence (Nested) Reverse gene specific primer WR GCACAGGAATACAACCAATACAAG ACTF ATCACAGCACTTGCACCAAGC Actin primer as Arabidopsis internal control, forward ACTR CCTTGGAGATCCACATCTGCTG Actin primer as Arabidopsis internal control, reverse whole promoter sequence (Outer)
