Supplementary Tables 1 Drought-responsive proteins leaves, leaf sheaths and root of the K86-161 and B34-164
cultivars that were identified by LC/MS-MS
Spot ID
Peptide sequence
Change in
expression
Source
Leaves K86-161 cultivar
1
2
3
73-85 -R.LASIGLENTEANR.Q
121-136 -R.KIVDILVEQGIVPGIK.V
122-136 -K.IVDILVEQGIVPGIK.V
122-136 -K.IVDILVEQGIVPGIK.V
122-139 -K.IVDILVEQGIVPGIKVDK.G
140-162 -K.GLVPLAGSNNESWCQGLDGLASR.E
178-192 -R.TVVSIPNGPSELAVK.E
178-200 -R.TVVSIPNGPSELAVKEAAWGLAR.Y
23-50 -R.IDQIIGPVLDITFPPGKLPNIYNALIVK.S
99-107 -R.IAQIPVSEAYLGR.V
108-128 -R.VINALAKPIDGRGEIVASESR.L
108-128 -R.VINALAKPIDGRGEIVASESR.L
142-162 -R-SVYEPLQTGLIAIDSMIPIGR.G
123-143 -K.QSQSNPLPVEEQVATIYTGTR
123-143 -K.QSQSNPLPVEEQVATIYTGTR.G
164-180 -K.HLKDTKPQFQEIISSSK.T
Zea mays
Saccharum hybrid
cultivar SP-80-3280
Zea mays
250-262 -R.VGLTALTMAEYFR.D
263-278 -R.DVNKQDVLLFIDNIFR.F
Oryza sativa
Japonica Group
5
82-92 -R.NNWVPCLEFSK.E
93-110 -K.EGFVYRENSTSPCYYDGR.Y
99-116 -R.ENSTSPCYYDGRYWTMWK.L
117-130 -K.LPMFGCTDASQVYK.E
131-145 -K.ELQEAIASYPDAYVR.I
131-145 -K.ELQEAIASYPDAYVR.I
146-153 -R.ILGFDNIR.Q
Clarkia xantiana
6
106-122 -R.WAMLGALGCVFPELLAR.N
106-122 -R.WAMLGALGCVFPELLAR.N
123-134 -R.NGVKFGEAVWFK.A
127-134 -K.FGEAVWFK.A
4
Spinacia oleracea
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaves K86-161 cultivar
7
8
9
10
11
94-109 -R.LYSIASSAIGDFGDSK.T
117-129 -K.RLIYTNDAGEIVK.G
160-178 -K.DPNATIIMLATGTGIAPFR.S
94-115 -R.WAMLAVPGIIVPEALGLGNWVK.A
94-115 -R.WAMLAVPGIIVPEALGLGNWVK.A
Zea mays
Solanum
lycopersicum
89-99 -K.WLAYGEVINGR.Y
164-176 -R.RLQDWYNPGSMGK.Q
177-184 -K.QYFLGLEK.F
185-207 -K.FLAGSGDPSYPGGPLFNPLG FGK.T
185-210 -K.FLAGSGDPSYPGGPLFNPLGFGKTEK.E
Zea mays
214-234 - K.QLVATGKPESFGGPFLVPSYR.G
214-234 - K.QLVATGKPESFGGPFLVPS YR.G
245-261 - R.GGSTGYDNAVALPAGGR.G
245-269 - R.GGSTGYDNAVALPAGGRGDEEELQK.E
Zea mays
143-154 - R.ANNFDLMYEQVK.A
143-154 - R.ANNFDLMYEQVK.A
185-198 - K.IFVIEGLHPMFDER.V
229-242 - R.GHSLESIKASIEAR.K
229-242 - R.GHSLESIKASIEAR.K
243-254 - R.KPDFDAYIDPQK.Q
311-320 - R.KLTCSYPGIK.F
362-372 - K.FYGEVTQQMLK.H
373-393 - K.HADFPGSNNGTGLFQTIVG LK.I
394-405 - K.IRDLYEQIVAER.A
Zea mays
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaves B34-164 cultivar
14
16
17
18
20
21
95-107 -R.IAQIPVSEAYLGR.V
95-107 -R.IAQIPVSEAYLGR.V
108-119 -R.VINALAKPIDGR.G
108-119 -R.VINALAKPIDGR.G
108-128 -R.VINALAKPIDGRGEIVASESR.L
108-128 -R.VINALAKPIDGRGEIVASESR.L
129-140 -R.LIESPAPGIISR.R
129-140 -R.LIESPAPGIISR.R
256-266 -R.HTLIIYDDLSK.Q
138-153 -R.LYSIASSAIGDFGDSK.T
138-153 -R.LYSIASSAIGDFGDSK.T
191-203 -K.ITGPVGKEMLMPK.D
204-222 -K.DPNATIIMLATGTGIAPFR.S
43-54 -K.QGADRQLWFASK.Q
89-99 -K.WLAYGEVINGR.Y
89-99 -K.WLAYGEVINGR.Y
164-176 -R.RLQDWYNPGSMGK.Q
165-176 -R.LQDWYNPGSMGK.Q
185-207 -K.FLAGSGDPSYPGGPLFNPLGFGK.T
185-207 -K.FLAGSGDPSYPGGPLFNPLGFGK.T
185-210 -K.FLAGSGDPSYPGGPLFNPLGFGKTEK.E
245-261 -R.GGSTGYDNAVALPAGGR.G
245-269 -R.GGSTGYDNAVALPAGGRGDEE ELQK.E
274-288 -K.NAASSTGNITLSVTK.S
289-313 -K.SNPETGEVIGVFESVQPSDTDLGAK.A
39-51 -K.VAASGSPWYGPDR.V
39-53 -K.VAASGSPWYGPDRVK.Y
122-129 -K.FGEAVWFK.A
39-54 -K.AKPAAASGSPWYGPDR.V
39-54 -K.AKPAAASGSPWYGPDR.V
104-120 -R.WAMLGALGCVFPELLAR.N
125-132 -K.FGEAVWFK.A
Oryza sativa
Japonica Group
Brachypodium
distachyon
Zea mays
Zea mays
Zea mays
Zea mays
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaves B34-164 cultivar
22
143-154 -R.ANNFDLMYEQVK.A
243-254 -R.KPDFDAYIDPQK.Q
362-372 -K.FYGEVTQQMLK.H
394-405 -K.IRDLYEQIVAER.A
394-405 -K.IRDLYEQIVAER.A
Zea mays
Leaf sheaths K86-161 cultivar
24
45-55 - R.AIAPLFVEHAK.K
79-89 - K.IEAMPTFHFIK.N
79-89 - K.IEAMPTFHFIK.N
90-101 - K.NGETVETIVGAR.K
Spinacia oleracea
25
13-23 -K.VNYYTPEYETK.D
24-32 -K.DTDILAAFR.V
138-150 -K.TFQGPPHGIQVER.D
Epilobium palustre
26
73-85 -R.LASIGLENTEANR.Q
323-331 -K.AAQDALLLR.A
343-359 -K.YTSDGEAAEAKEGMFVK.N
28
29
132-143 -K.KLCLEPTSFTVK.A
133-143 -K.LCLEPTSFTVK.A
206-213 -R.VPFLFTVK.Q
214-234 -K.QLVATGKPESFGGPFLVPSYR.G
214-234 -K.QLVATGKPESFGGPFLVPSYR.G
245-261 -R.GGSTGYDNAVALPAGGR.G
245-261 -R.GGSTGYDNAVALPAGGR.G
60-72 -R.AGGVDDLPLVGNK.A
163-171 -K.YPLISDVTK.S
163-175 -K.YPLISDVTKSISK.A
197-212 -K.EGVIQHSTINNLAIGR.S
Oryza sativa
Japonica Group
Zea mays
Zea mays
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaf sheaths K86-161 cultivar
30
31
67-78 -R.LSGVTAEGLGVK.V
165-177 -R.SVQMEGLLWGASK.L
72-82 - K.ALEQLDAAVAK.G
97-110 - K.FNGGGHVNHSIFWK.N
111-126 - K.NLKPISEGGGEPPHGK.L
111-126 - K.NLKPISEGGGEPPHGK.L
168-183 - K.KLSVETTANQDPLVTK.G
168-183 - K.KLSVETTANQDPLVTK.G
206-217 - K.NVRPDYLNNIWK.V
206-217 - K.NVRPDYLNNIWK.V
32
73-86 -R.FYAISAEYPEFSNK.D
73-88 -R.FYAISAEYPEFSNKDK.T
33
54-71 -K.SSVTANDFYYHGLAGQGK.I
54-71 -K.SSVTANDFYYHGLAGQGK.I
78-100 -K.AAVTPAFVGQFPGVNGLGISAAR.L
78-100 -K.AAVTPAFVGQFPGVNGLGISAAR.L
78-100 -K.AAVTPAFVGQFPGVNGLGISAAR.L
201-211 -K.VTFLDDAQVK.K
202-212 -K.VTFLDDAQVKK.L
35
72-85 -K.RLASIGLENTEANR.Q
73-85 -R.LASIGLENTEANR.Q
121-136 -R.KIVDILVEQGIVPGIK.V
122-136 -K.IVDILVEQGIVPGIK.V
122-136 -K.IVDILVEQGIVPGIK.V
140-162 -K.GLVPLAGSNNESWCQGLDGLASR.E
140-162 -K.GLVPLAGSNNESWCQGLDGLASR.E
178-192 -R.TVVSIPNGPSELAVK.E
36
138-150 -K.TFQGPPHGIQRER.D
Zea mays
Zea mays
Hordeum vulgare
subsp. vulgare
Sorghum bicolor
Zea mays
Carex albursina
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaf sheaths B34-164 cultivar
37
38
39
142-157 -R.LYSIASSALGDFGDSK.T
166-177 -R.LVYTNENGELVK.G
178-194 -K.GVCSNFLCDLKPGAEVK.I
60-72 -R.AGGVDELPLVGNK.A
155-171 -K.SGGLGDLKYPLISDVTK.S
163-171 -K.YPLISDVTK.S
190-196 -R.GLFIIDK.E
197-212 -K.EGVIQHSTINNLAIGR.S
82-92 -R.NNWVPCLEFSK.E
117-130 -K.LPMFGCTDASQVYK.E
117-130 -K.LPMFGCTDASQVYK.E
131-145 -K.ELQEAIASYPDAYVR.I
131-145 -K.ELQEAIASYPDAYVR.I
146-153 -R.ILGFDNIR.Q
Glycine max
Brachypodium
distachyon
Miscanthus x
giganteus
Medicago truncatula
40
318-330 -K.LIALQEGMLVGISSGAAK.R
41
1-16 -.MAYVLISDLMYGDTDK.R
Oryza sativa
Japonica Group
144-123 -K.EREFPGQVLR.Y
144-165 -K.KTITEYGSPEEFLSQVDFLLGK.Q
144-165 -K.KTITEYGSPEEFLSQVDFLLGK.Q
145-165 -K.TITEYGSPEEFLSQVDFLLGK.Q
145-165 -K.TITEYGSPEEFLSQVDFLLGK.Q
200-209 -K.QYYSVSVLTR.T
210-230 -R.TADGDEGGKHQLITATVSDGK.L
Sorghum bicolor
42
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Leaf sheaths B34-164 cultivar
43
109-116 -K.QATPSDPR.T
Populus trichocarpa
Root K86-161 cultivar
46
47
48
49
50
44-55 -K.DDGTLASYVGFR.V
244-255 -K.NVDGLDIAQLVK.H
390-399 -R.MGAFTLGVNR.V
Zea mays
25-38 -K.GILAADESTGTIGK.R
40-53 -R.LSSINVENVEENRR.A
85-94 -K.DGKPFVDVLK.E
137-149 -K.KVTPEVIAQYTVR.T
Zea mays
111-126 -K.NLKPISEGGGEPPHGK.L
163-183 -K.KLSVETTANQDPLVTK.G
Zea mays
59-72 - K.SSVHDVVLVGGSTR.I
328-339 - K.ELESLCNPIIAK.M
Zea mays
65-74 - K.KANALLGYEK.L
117-130 - K.ELQEFKPVEEGASA.-
Sorghum bicolor
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Root K86-161 cultivar
51
52
53
240-255 - K.VVTIDSYEDVPANSEK.S
340-351 - K.CGIAVEPSYPLK.K
113-120 - R.FRLPENAK.T
126-139 -R.AALENGVLTVTVPK.A
17-33 - K.LAEQAERYEEMVEFMEK.V
17-33 - K.LAEQAERYEEMVEFMEK.V
49-56 - R.NLLSVAYK.N
85-92 - R.VTLIKDYR.G
109-124 - K.LLESHLVPSSTAPESK.V
109-124 - K.LLESHLVPSSTAPESK.V
137-147 - R.YLAEFKTGAER.K
Zea mays
Zea mays
Zea mays
54
91-104 -K.KYVYTIDDDCFVAK.N
186-195 -R.YVDAVMTIPK.G
163-171 -K.ASNPFVNLR.K
Chlorella variabilis
57
25-39 - K.GILAADESTGTIGKR.L
85-94 - K.DGKPFVDVLK.E
237-249 - K.KVTPEVIAQYTVR.T
237-249 - K.KVTPEVIAQYTVR.T
238-249 - K.VTPEVIAQYTVR.T
Zea mays
58
73
72-82 -K.ALEQLDAAVAK.G
97-110 -K.FNGGGHVNHSIFWK.N
97-110 -K.FNGGGHVNHSIFWK.N
111-126 -K.NLKPISEGGGEPPHGK.L
168-183 -K.KLSVETTANQDPLVTK.G
169-183 -K.LSVETTANQDPLVTK.G
206-217 -K.NVRPDYLNNIWK.V
223-233 -K.YAGEVYENVLA.-
Saccharum
officinarum
27-45 - R.SIVPSASGDSETAAFANAR.I
27-45 - R.SIVPSASGDSETAAFANAR.I
Sorghum bicolor
Supplementary Tables 1 continued
Spot ID
Peptide sequence
Change in
expression
Source
Roots B34-164 cultivar
59
72
74
12-28 - K.LAEQAERYEEMVEYMEK.V
32-43 - K.TVDVEELTVEER.N
44-51 - R.NLLSVAYK.N
90-103 - K.IEAELSNICDGILK.L
155-171 - K.AAQDIALAELAPTHPIR.L
155-171 - K.AAQDIALAELAPTHPIR.L
80-93 - R.FYAISAEYPEFSNK.D
80-95 - R.FYAISAEYPEFSNKDK.T
128-148 - K.FGGDTPYSIMFGPDICGYSTK.K
198-212 - K.QTGSIYEHWDILPPK.E
198-212 - K.QTGSIYEHWDILPPK.E
5-12 -K.FFVGGNWK.C
101-113 -R.ALLGESNEFVGDK.V
124-135 -K.VIACVGETLEQR.E
176-190 -K.VATPAQAQEVHASLR.D
176-190 -K.VATPAQAQEVHASLR.D
Saccharum hybrid
cultivar CP65-357
Triticum aestivum
Zea mays