uv sci
Photochemotherapeutic effects of UV-C on acridine orange in human breast cancer cells: potential application in anticancer therapy
By: Pitchaimani, A (Pitchaimani, Arunkumar) [ 1 ] ; Renganathan, A (Renganathan,
Arun) [ 1 , 3 ] ; Cinthaikinian, S (Cinthaikinian, Selvamurugan) [ 2 ] ; Premkumar, K (Premkumar, Kumpati) [ 1 ]
RSC ADVANCES
Volume: 4
Issue: 42
Pages: 22123-22128
DOI: 10.1039/c4ra01647e
Published: 2014
View Journal Information
Abstract
The photochemical effects of Ultra Violet-C (UV-C) radiation on the fluorochrome, acridine orange
(AO) in human breast cancer cells (MCF-7) has been investigated for effective anticancer therapy.
Fluorescence enhancement of AO under UV-C irradiation has been evaluated in a cell free environment using fluorescent spectral studies. Further, its photo-toxic effects were assessed using
MTT assay, NBT assay and apoptotic studies through Hoechst staining and fluorescent activated cell cycle analysis. The results demonstrate that AO under UV-C exhibits enhanced fluorescence and dose dependent cytotoxicity with significant ROS production at higher doses compared to light and dark toxicity. Apoptotic studies reveal that higher exposure to UV-C of intracellular AO significantly increases the Sub-G1 fraction and morphological changes were distinct compared to the control cells.
These results demonstrate that UV-C could be an ideal light delivery system for AO mediated photochemotherapy and can be utilized for effective anti-cancer therapy.
Keywords
KeyWords Plus: PHOTODYNAMIC THERAPY ; INDUCED APOPTOSIS ; ARGON-
LASER ; MECHANISMS ; RADIATION ; PATHWAYS
Author Information
Reprint Address: Premkumar, K (reprint author)
Bharathidasan Univ, Sch Basic Med Sci, Dept Biomed Sci, Canc Genet & Nanomed Lab, Tiruchchirappalli 620024, Tamil Nadu, India.
Addresses:
[ 1 ] Bharathidasan Univ, Sch Basic Med Sci, Dept Biomed Sci, Canc Genet & Nanomed Lab, Tiruchchirappalli 620024, Tamil Nadu, India
[ 2 ] Pavendar Bharathidasan Coll Engn & Technol, Dept Biotechnol, Tiruchchirappalli 620024, Tamil Nadu, India
[ 3 ] Univ Zurich Hosp, Clin Oncol, Mol Oncol Lab, CH-8044 Zurich, Switzerland
E-mail Addresses: pkumpati@hotmail.com
Funding
Funding Agency Grant Number
Department of Biotechnology
Department of Science Technology
Government of India, Ministry of Science and Technology, New Delhi,
India
View funding text
BT/PR12864/NNT/28/442/2009
SR/FT/LS-004/2009
Publisher
ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD,
CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Categories / Classification
Research Areas: Chemistry
Web of Science Categories: Chemistry, Multidisciplinary
Document Information
Document Type: Article
Language: English
Accession Number: WOS:000337123100050
ISSN: 2046-2069
Other Information
IDS Number: AI8AB
Cited References in Web of Science Core Collection: 28
Times Cited in Web of Science Core Collection: 0
