The University of Southern Mississippi Laboratory Management Plan
Section 1
Table of Contents
Safety Program Key Personnel
Section 2 Laboratory Contact Information
Section 3
Section 4
Section 5
Section 6
Emergency Procedures
Lab and Building-Specific Evacuation Information
Chemical/Biological Inventory
Material Safety Data Sheets (MSDSs)
Section 7
Section 8
Section 9
Laboratory-Specific Operating Procedures (SOPs)
Employee Training
Inspections and Exposure Monitoring Records
Section 10 Incidents, Injuries and Corrective Actions
Section 11 Chemical Waste Requirements
Section 12 Radiological Hazards
Section 13 Biological Hazards
Section 14 Laboratory Animals
Section 15 Safety Program Correspondence
Section 16 Personal Protective Equipment (PPE) Assessment
Section 17 Hazard Assessment Information and PPE Selection Information
Section 18 Laboratory Safety Handbook
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Section 1 – Safety Program Key Personnel
In addition to the following personnel, please include your department's safety program key personnel.
Name
Lynn Landrum
Position Phone Email
Director,
EHS
266-6912 lynn.landrum@usm.edu
Martha Sparrow Assistant Director,
EHS
266-4045 martha.sparrow@usm.edu
Lynn Landrum Chemical Safety Officer
Chemical Safety (EHS)
266-6912 lynn.landrum@usm.edu
Lynn Landrum Radiation Safety Officer
Radiation Safety (DRS)
266-4045 martha.sparrow@usm.edu
Lynn Landrum
EHS
Bio-Safety Officer 266-6912 lynn.landrum@usm.edu
Lynn Landrum
Lynn Landrum
EHS
EHS
Department Safety Contact:
OSHA Lab Safety Compliance 266-6912 lynn.landrum@usm.edu
Lab Environmental Compliance 266-6912 lynn.landrum@usm.edu
(Phone) (Email) (Name)
Lab Safety Contact:
(Position)
(Name) (Position) (Phone) (Email)
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Section 2 – Laboratory Contact Information
This Laboratory Management Notebook pertains to the laboratories and the research facilities of:
Laboratory Room(s) and Building: Laboratory Phone Number(s):
Name of Principal Investigator: Emergency Phone Number(s):
Name of Lab Safety Contact (if different than PI) :
Names of Other Lab Personnel and Users:
Emergency Phone Number(s):
Emergency Phone Number:
List all rooms related to the above mentioned laboratory / research facility. Include all rooms such as temperature controlled rooms, storage closets, and animal facilities associated with laboratory activities.
Indicate the faculty who currently direct the indicated rooms.
Related Room(s): Faculty Supervisor: Phone Number(s):
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Section 3 – Emergency Procedures
Each laboratory group should develop plans and procedures for dealing with emergency situations. As a minimum, this should include emergency phone numbers (see above) and evacuation plans. Also refer to the
USM Emergency Response Plan for additional information.
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Section 4 – Lab and Building-Specific Evacuation Information
To report an emergency: dial 911 from a campus phone
( 911 from a cell phone )
Building Manager / Emergency Coordinator:______________________________________
Supervisor(s) for this area:
Designated assembly areas:
______________________________________
______________________________________
______________________________________
______________________________________
______________________________________
Example Emergency Egress Map
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Section 5 – Chemical Inventory
Each laboratory is responsible for taking and maintaining its own chemical inventory. USM requires that all chemical inventories be available on the Chem Tracker database.
A chemical inventory report in Chem Tracker will have a clear title and heading, which includes the following information:

Room number and building name

Department

PI/owner of the inventory

Barcodes of the chemicals
The reports can be downloaded in an Exel format for internal use by the laboratories. Principal Investigators should also consider their own needs for chemical management. Management of the the inventory rests with the laboratory. All chemicals received by the USM chemical storeroom. The barcode is added at that time. In order to maintain inventory management it is the labs responsibility to insure the barcodes are removed from empty containers and returned to EHS. A chemical inventory should strive to identify the following:

An accurate chemical name

Approximate quantity

Basic hazard information or classification

A specific laboratory location

Annual chemical usage

If quantity changes significantly
The Hazard Classifications can be identified with notations such as the following (suggested by EHS):
BIO
CARC
CG
CMB
COR-A
COR-B
EXP
F
HTX
Biohazard
Carcinogen
Compressed Gas
Combustible
Corrosive - Acid
Corrosive – Base/Caustic
Explosive
Flammable
Highly Toxic
NON
OX
PF
PYR
R
RAD
RTX
SEN
TOX
Non-Hazardous
Oxidizer
Reactive
Toxic
Peroxide-Former
Pyrophoric
Radioactive
Reproductive Toxin
Sensitizer
IR Irritant WR Water-Reactive
A chemical may belong to more than one Hazard Class. It is the responsibility of the Principal Investigator to determine if chemicals in use or in storage produce a potential hazard that must be identified on the Chemical
List.
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Section 6 – Material Safety Data Sheets (MSDSs)
Each lab is responsible for training all personnel in the lab in how to obtain a of relevant MSDSs. MSDSs and safety information can be obtained through the manufacturer or Chem Tracker. Insure the MSDSs correlate to the chemical manufacturer and composition. Most MSDSs can be quickly found through links to the chemical manufacturer's web site. EHS can assist in obtaining MSDSs and safety information that are difficult to find.
EHS suggests that if particularly hazardous materials are used that a hard copy of the MSDS be kept with the
SOP. If there are a large number of MSDSs, then it may be appropriate to place them in a separate MSDS
Notebook, to be kept in a specified location.
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Section 7 – Laboratory-Specific Operating Procedures (SOP)
Each laboratory working with hazardous chemicals should include their own specific Specific Operating
Procedures (SOP) here. This section can include laboratory-specific procedures for the following subjects, as they apply for each laboratory.

Working with carcinogens or reproductive toxins

Chemical procurement, distribution, and storage

Housekeeping, maintenance, and inspections

Selection, use and disposal/laundering of protective apparel and equipment

Dealing with hazardous procedures, substances, and/or equipment

Working with radioisotopes

Working with biologically hazardous materials

Operations requiring special approval prior to being conducted

Any additional materials, special equipment instructions, experimental procedures, or precautions/procedures associated with unique hazards
The SOP template which follows is intended as a resource – you may adjust the format for your usage. The underlying rule in writing an SOP is to convey the required information in the most functional way.
Please list the SOP titles pertinent to this laboratory:
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LABORATORY SPECIFIC OPERATING PROCEDURE
(for the use of hazardous materials or equipment)
NAME OF PROCEDURE:
PREPARED BY: REVISION DATE:
LOCATION – This procedure may be performed at the following location(s):
HAZARDS – The materials and equipment associated with this procedure present the following exposure or physical health hazards. Safety precautions are prudent and mandatory:
ENGINEERING CONTROLS – Prior to performing this procedure, the following safety equipment must be accessible and ready for use, e.g., chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits, etc.
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PROTECTIVE EQUIPMENT – Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use, e.g., acid resistant gloves, safety eyewear, lab coat, chemical splash apron, etc.
WASTE DISPOSAL – This procedure will result in the following regulated waste which must be disposed of in compliance with environmental regulations:
ACCIDENTAL SPILL – In the event that a hazardous material spills during this procedure, be prepared to execute the following emergency procedure:
PRIOR APPROVAL – This procedure is considered hazardous enough to warrant prior approval from the Principal Investigator.
- YES - - NO -
CERTIFICATION – I have read and understand the above SOP. I agree to contact my Supervisor or
Lab Manager if I plan to modify this procedure.
Signature Name (Print) Date Building & Room #
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Section 8 – Employee Training
The Principal Investigator / Laboratory Supervisor is responsible for ensuring that all laboratory personnel get the appropriate safety training. This may be achieved by using the “USM Laboratory Safety Handbook” available in section 18 and in the resource section of the EHS web site. General safety training may also be obtained by taking the CHE/PSC 410/510 class. This should be supplemented with further instruction on sitespecific chemical hazards and safety procedures, as determined by the Principal Investigator / Laboratory
Supervisor.
By law all laboratory personnel should receive training on how to protect themselves from the hazards present at their locations. Research laboratories are hazardous workplaces where highly skilled personnel carry out finely detailed work. As such it is important that all laboratory personnel are competent to perform their research activities effectively, and competent to do so safely – without damaging equipment, without harming themselves and without harming their neighboring researchers.
Documented training serves as one building block of competence, together with verbal and hands-on instruction. Documentation is a necessary part of an effective training program, although it is no guarantee of personnel competence. Naturally, on-going interaction with knowledgable researchers should continue beyond the documented phases of training to ensure the proper skills are imparted to new research personnel.
Safety training records should be kept in this section for each person using the laboratory, including the
Principal Investigator. Records should minimally include the person trained, the type of training (e.g.,General
Lab Safety Lecture, a laboratory-specific training topic), the trainer (e.g., EHS, Principal Investigator), and the training date.
As examples of lab-specific training records, three different forms are provided on the pages which follow.
These forms serve slightly different functions and may be used as needed:
Employee-Specific Chemical Hygiene Training Form
The concept behind this form is the personal training file, a collection of the training received by an individual.
Under “Description of Training” reference can be made to other documentation if more than a short description is desired. However, if training records are organized only by person, it becomes difficult to search training records by topic.
Safety Training Checklist
The Safety Training Checklist provides a structure for covering a set of predetermined topics in a training program. It is particularly useful for conducting orientation training, as it helps remind the trainer about topics to cover. Each item on the checklist can point to other documents which describe the training content. If the checklist provided here is used, trainer and trainee can initial and date points as they are completed.
Additionally, not all points of the checklist need apply to all personnel, and certain points may be reserved until they are applicable for an individual.
Training Sign-in Sheet
A sign in sheet is appropriate for recording all attendees at a large training session. However, if training records are organized only by topic, it becomes difficult to answer the question, “What training has a given person received?”
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Employee Specific Laboratory Management Plan Training Form
Department Name
Campus Location
Employee Classification
Campus Phone
Supervisor
OSHA's Laboratory Standard (29 CFR 1910.1450) requires that each laboratory employee be made aware of the location and content of the laboratory's Chemical Hygiene Plan(USM Laboratory Management Plan). By your signature below, you acknowledge that you have read and understood the contents of this plan and know its location within the laboratory.
Employee Signature Date
The employee’s supervisor is required to provide training on specific topics as described under the
“Information and Training” section of the Lab Standard. This training must be provided at the time of the employee's initial assignment, on a refresher basis as determined by the employer, and upon updating procedures. Document specific employee training below:
Description of Training Date Provided By
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SAFETY TRAINING CHECKLIST
This checklist may be used to assist employers with the laboratory-specific training requirements outlined in the
USM Laboratory Management Plan.
Introduction to laboratory-specific Laboratory Management Plan (LMP):
Location and contents
Review Chemical Inventory in the Chem Tracker database.
Review method of obtaining MSDSs.
Review emergency information: Spills, Personal Injury, Fire, and Power Failure.
Fire extinguisher
First aid supplies
Safety shower
Eye wash
Evacuation plans
Basic Safety Rules
Note rules with special importance for your laboratory.
Identify specific areas for food consumption outside of the lab.
Review procedures for working after hours. (no chemical processes alone)
Review Waste Handling Procedures.
Labeling
Packaging
Pick-ups
Review procedures for chemical procurement, distribution, and storage.
Review Laboratory Specific Operation Procedures for use of hazardous materials
Storage (acid cabinet, flammable liquid storage cabinet, flammable liquid storage refrigerator, etc.)
Personal Protective Equipment (PPE)
Location where certain procedure(s) may be performed (e.g., mechanical ventilation required)
Waste Disposal (aqueous, solid, biohazardous, and radioactive)
Review procedures for use of compressed gas cylinders
Protective Apparel and Equipment
Discuss when safety glasses, goggles, or face shields are required.
Discuss any need for other protective equipment.
Discuss selection of gloves.
Housekeeping, Maintenance, and Inspections
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Discuss materials stored or frequently present on the floor.
Discuss maintenance items for scientific equipment.
Discuss formal and internal inspection programs.
Exposure Monitoring
Discuss PEL and TLV for chemicals in use and how to reduce employee exposure.
Discuss building ventilation.
Discuss use of fume hoods, biological safety cabinets or other mechanical ventilation systems.
Review SOP for working with Biologically Hazardous Materials
Review Exposure Control Plan if working with human blood or other potentially infectious materials.
Discuss Biosafety Requirements if working with recombinant DNA or infectious agents.
Review Hepatitis B Vaccination Program.
Review PPE, Housekeeping and Waste Disposal Procedures.
Working with Radioisotopes
Review Radiological Safety Practices.
Review Dosimetry Program.
Medical Program
Review criteria for medical surveillance, as found in the USM Laboratory Safety Handbook.
Training Program
Discuss Unit-Specific, EHS and other training sessions.
Additional Safety Session Topics
Review recent incidents/accidents/injuries and how to prevent reccurrence.
Review new equipment at least annually.
Review new procedures at least annually
Review results of recent inspections and how to correct problem areas.
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TRAINING SIGN-IN SHEET
I, (print full name) , certify that the following affected employees have received and understood the following training (please describe training content below):
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
__________________________________________________________________________________
Name ID Number Signature Date
(signature) (date)
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Section 9 – Inspection and Exposure Monitoring Records
This section should contain information on maintenance inspections, laboratory inspections and exposure monitoring (including the date and recommendations). Inspections may be conducted by individual research groups, EHS, DOL, NRC, and possibly other organizations. This section should also contain records associated with corrective actions. The Equipment Inspection form and Laboratory Inspection form below may be used.
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EQUIPMENT INSPECTION FORM
Periodic maintenance & housekeeping self-inspections are recommended for significant pieces of equipment.
Maintenance inspections should include equipment operability, structural integrity, and safe guards. Records should note who is responsible for the inspection, how often inspections will be conducted and where additonal records are kept.
Inspector:
Date of Inspection:
Location of Records:
Equipment Comments / Action Satisfactory Unsatisfactory
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USM Laboratory Safety Inspection Form
Location:
PI Name:
Supervisor Name:
Department:
Today’s Date:
Inspector Name:
Inspector Title:
Department:
Phone/E-mail: Phone/E-mail:
Refer to the Instructions for the Laboratory Safety Inspection Form what to look for when checking each item.
for a more detailed description as to
S=Satisfactory U=Unsatisfactory N=Not Applicable
GENERAL SAFETY
Door Signs
1 Key lab personnel are identified.
2 Hazard information sections are completed.
3 Information is current and applicable to the lab.
Housekeeping
4 Lab door is able to be secured.
5 View through door’s window is unobstructed.
6 Aisles and exits are unobstructed.
7 Work areas are uncluttered.
8 Storage areas are uncluttered.
9 Ceiling tiles are in place.
Equipment
10 Equipment manuals are available.
11 Equipment is unobstructed.
12 Guards are in place.
13 Equipment is secured or on a stable surface.
14 Auto shut-offs are on equipment.
ELECTRICAL SAFETY
15 Emergency cut-off switches are accessible.
16 Electrical panels are accessible.
Electrical Outlets
17 Outlets are not overloaded.
18 GFCI outlets are present.
Electrical Cords
19 Electrical cords are clear of aisles, sinks and heat sources.
20 Electrical cords are in good condition.
Extension Cords
21 Extension cords are used temporarily (less than 90 days).
22 Extension cords are of 3-wire design, with ground plug intact.
EMERGENCY EQUIPMENT
23 Emergency contact information is posted.
24 Fire alarm pull stations are unobstructed.
25 Stocked first aid kit is available.
26 Stocked spill clean-up kit is available.
COMMENTS
S U N
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EMERGENCY EQUIPMENT continued
Fire Extinguishers
27 Extinguishers are correct type for the hazards present.
28 Extinguishers are mounted to the wall or in extinguisher cabinet.
29 Extinguishers are unobstructed.
30 Pressure gauges are in the green.
31 Extinguisher has not been used.
32 Extinguisher is not damaged or corroded.
33 Extinguisher has been tagged within the last year.
34 Extinguisher has been inspected within the last month.
Safety Shower and Eyewash
35 Safety Shower/Eyewash is unobstructed.
36 Safety Shower/Eyewash location identified by a highly visible sign.
37 Safety Shower/Eyewash is tested regularly.
CHEMICAL SAFETY
38 Spark producing equipment is kept away from flammables.
39 No evidence of eating or drinking is in the lab.
40 House vacuum is protected by traps/filters.
Laboratory Management Plan (LMP)
41 Current LMP is available.
42 Lab-specific safety information is included.
43 Lab personnel are trained on the LMP.
44 Material Safety Data Sheets are available.
45 Chemical inventory is current and available.
Personal Protective Equipment
46 Eye/face protection is worn while in the lab.
47 Labcoats or aprons are worn.
48 Appropriate gloves are worn.
49 Respirator wearers have received approval, training and fit-testing.
Chemical Storage
50 Chemicals are stored per compatibility.
51 Chemicals are stored in cabinets or stable shelving.
52 Chemicals are stored in proper containers.
Chemical Containers
53 Containers are in good condition.
54 Containers are properly labeled.
55 Containers are closed when not in use.
56 Secondary containment is used for waste containers
Gas Cylinders
57 Gas cylinders are securely fastened to prevent tipping.
Peroxidizable Chemicals
58 Peroxidizables are dated when opened.
59 Peroxidizables are tested for peroxides every six months.
CHEMICAL SAFETY continued
Peroxidizable Chemicals
60 Refrigerator is explosion-proof/-protected.
61 Flammable cabinets are used.
62 NFPA Maximum allowable storage volumes are not exceeded.
Mercury Devices
63 Mercury-containing devices have been replaced or removed.   
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64 No mercury thermometers are used in ovens.
Chemical Fume Hoods
65 Chemical fume hoods have been inspected within the last year
66 Air flow into the hood is unobstructed.
BIOLOGICAL SAFETY
1 Functional sink is in laboratory space.
2 Exterior windows are sealed shut, or have fly screens present.
3 Bio-safety cabinet(s) have been certified within the last year.
4 Lab space can be easily decontaminated.
5 Appropriate disinfectant for material is in lab.
Bench Tops
6 Bench tops are made of an impervious material.
7 Bench tops are resistant to chemicals and moderate heat.
Laboratory Furniture
8 Furniture is appropriately sturdy.
9 Furniture can be easily decontaminated.
Biohazard Waste Containers
10 Containers are able to be closed.
11 Containers display the biohazard symbol.
12 Containers are appropriate for transport to autoclave.
13 Containers are closed when not in use.
14 Sharps Disposal Containers are present.
RADIATION SAFETY
1 Weekly contamination surveys are conducted.
  
  
  
3 Equipment for detecting radiation hazards is readily available.
4 Material is secured when unattended.
Radioactive Materials Recordkeeping
5 Inventory of the lab's radioisotopes is available.
6 Records of quantities used are available.
Radioactive Waste Containers
8 Waste container is of appropriate material.
9 Wastes are segregated per half-life.
10 Waste container is properly labeled.
11
Containment and Shielding
12 Workspace is lined with absorbent paper.
13 Shielding is used for highly radioactive materials.
14 Tools are used for highly radioactive materials.
  
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Lasers
15 Class IIIB or IV lasers are registered.
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The following briefly describes what EHS looks for during a typical laboratory inspection (for general laboratory safety and chemical safety – biological and radiation safety issues are only briefly addressed). The categories follow the form point by point.
General Safety
Door Signs
Emergency responders (e.g., Fire Department, Paramedics, Police) need information on the hazards present before entering a room. The EHS Department provides the officially approved signs for posting laboratory contact information and listing special hazards. (Contact the EHS Department to request additional information at 601-266-6912 or 601-266-
4045. The sign should be posted either next to or on the door, or taped to the door in a way that it is visible to emergency personnel.
1.
Key lab personnel are identified.
The names and phone numbers (office and home) of the principal investigator and other knowledgeable employees/students should be listed on or beside the door.
2.
Hazard information sections are completed.
The appropriate hazard information should be on the sign.
3.
The information is current and applicable to the lab.
All door signs should be checked for accuracy at least annually. Additionally, the information should be updated whenever the contact or hazard information changes. Door signs should be dated when posted or updated, or at the time of the annual check.
Housekeeping
4.
Lab door is able to be secured.
All doors that enter from the public areas of the building should be able to be locked to secure the laboratory.
5.
View through door’s window is unobstructed.
If the laboratory door is outfitted with a window, it needs to remain unobstructed for emergency responders to view the laboratory prior to entry.
6.
Aisles and exits are unobstructed.
There should be no objects that block movement through aisles or exits. Emergency personnel should be able to access all areas of the laboratory through all exit doors, and should be able to move freely in the aisles when smoke may be present.
7.
Work areas are uncluttered.
Floor space and bench space should not be cluttered with excessive storage. Physical hazards should be minimized
(e.g., tripping hazards, items that could fall on someone, etc.), and combustible/flammable materials should not be stored in excessive amounts.
8.
Storage areas are uncluttered.
Materials should be stored in such a way that they will not accidentally slide, collapse, fall, or spill.
9.
Ceiling tiles are in place.
Ceiling tiles should be kept intact and in place as part of the ventilation scheme of the room.
Equipment
10.
Equipment manuals are available.
Safe operating instructions (usually from the manufacturer) should be available as part of the Specific Operating
Procedures required by the USM Laboratory Management Plan.
11.
Equipment is unobstructed.
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Clutter should be limited to keep equipment readily accessible and operable.
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12.
Guards are in place.
The Point-of-Operation of any piece of equipment must have a guard. Examples include:
 Nip or Pinch Points
 between two rotating parts;
 between a rotating part and a stationary part;
 or between a rotating part and longitudinal motion
 Hazardous projections on rotating parts
 Hazardous reciprocating motion
Belt-driven equipment should have a guard in place, completely covering the belt and pulley.
13.
Equipment is secured or on a stable surface.
Equipment should be set up or stored in such a way that it will not accidentally fall or tip over.
14.
Auto shut-offs are on equipment.
Any equipment that is used when unattended should have an automatic shut-off to prevent situations that might result in fire or other emergencies. An example is a heating bath over-temperature shut-off (for when the water completely evaporates).
Electrical Safety
15.
Emergency cut-off switches are accessible.
Access to cut-off switches should not be obstructed. Three-foot clearances should be maintained around all emergency pull switches. Striped tape applied to the floor is recommended to visually mark the clearance area.
16.
Electrical panels are accessible.
Access to electrical panels should not be obstructed. Three-foot clearances should be maintained around all circuit panel boxes. Striped tape applied to the floor is recommended to visually mark the clearance area.
Electrical Outlets
17.
Outlets are not overloaded.
Electrical circuits should not be overloaded. “Overloaded” is defined as excessive electrical cords plugged into a circuit through the use of adapters, which allow multiple plug capability. Powerstrips with circuit breakers can be used, but should not be used in a series or with adapters. Three-to-two-prong adapters should never be used to plug a three-wire plug into a two-wire system.
18.
GFCI outlets are present.
Ground Fault Circuit Interrupter (GFCI) protection must be used for electrical appliances that will be operated within six feet of water.
Electrical Cords
19.
Electrical cords are clear of aisles, sinks and heat sources.
20.
Electrical cords are in good condition.
Cords should be inspected for any damage. Cords with damage to the insulation (i.e., wires are visible or tape is needed to hold it together) or frayed cords should be replaced immediately. Equipment with cords that have obvious shorts should also be taken out of service until the cords are replaced.
Extension Cords
21.
Extension cords are used temporarily (less than 90 days).
Electrical equipment should be located so that extension cords are not needed on a permanent basis or an outlet should be installed close to the equipment. Power strips with circuit breakers are acceptable to use, but should not be connected in a series or to an extension cord.
22.
Extension cords are of 3-wire design, with the ground plug intact.
All extension cords must be properly grounded three-wire cords. This includes having the ground plug intact.
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Emergency/Safety Equipment
23.
Emergency contact information is posted by the telephone.
Emergency phone numbers (including 911) should be posted by or on all phones in the laboratory.
24.
Fire alarm pull stations are unobstructed.
25.
Stocked first aid kit is available.
The first aid kit should be available and unobstructed. Consider the various injuries that could happen in the laboratory, and assemble the first aid kit accordingly. For example, if hydrofluoric acid is used in the laboratory, then calcium gluconate gel should be available for the first aid treatment of skin exposure to hydrofluoric acid. Personnel should be trained on the proper use of the kit’s contents. Periodically check expirations on any ointments or overthe-counter pharmaceuticals. Periodically evaluate the hazards present in the laboratory, and adjust the first aid kit contents as needed.
26.
Stocked spill clean-up kit is available.
A spill clean-up kit should be available and unobstructed. Consider the spills that could happen in the laboratory, and assemble the spill clean-up kit accordingly. Materials should be evaluated for compatibility with the hazards in the laboratory that could be spilled. Universal sorbents, such as 3M Powersorb, and spill pads are recommended for spill kits.
If any materials are used, they should be re-stocked immediately. Periodically evaluate the spill clean-up kit, and adjust the kit contents as needed.
Fire Extinguishers
27.
Extinguishers are correct type for the hazards present.
In general, fire extinguishers suitable for the hazard to be protected should be available. There are five types of fires:
 Class A: Ordinary combustibles such as wood, paper, cloth, trash and plastics.
 Class B: Fires in flammable liquids (such as gasoline, petroleum and paint) and flammable gases (propane, butane, etc.).
 Class C: Energized electrical equipment, such as motors, transformers and appliances.
 Class D: Combustible metals, such as potassium, sodium, aluminum and magnesium.
 Class K: Cooking oil fires.
Most labs should have an ABC type of extinguisher, as they work on the most common types of fires. If there are
Class D or K fire hazards, appropriate extinguishers should also be present. For additional information contact Fire
Safety at 601-266-4414.
28.
Extinguishers are mounted to the wall or in extinguisher cabinet.
Fire extinguishers should be mounted on the wall, or in an extinguisher cabinet, not sitting on the floor.
29.
Extinguishers are unobstructed.
Fire extinguishers should be in an obvious and accessible location near the exit door and/or near the hazard.
30.
Pressure gauges are in the green.
Check indicators (if present) on fire extinguishers to be sure that the pressure gauge is in the normal range. If the indicator is not in the normal range, the extinguisher needs to be serviced through Fire Safety at 601-266-4414
31.
Extinguisher has not been used.
Make sure the pin is in place on the extinguisher handle and the tie unbroken. If there is any indication of usage, the extinguisher needs to be serviced through Fire Safety at 601-266-4414).
32.
Extinguisher is not damaged or corroded.
Confirm that seals or tamper indicators are intact. Check that extinguisher operating instructions are legible and face outward. Note any obvious physical damage. Confirm that the Hazardous Material Identification System label is in place.
33.
Extinguisher has been tagged in the last year.
The fire extinguisher should be tagged with an inspection date within the last year.
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34.
Extinguisher has been inspected within the last month.
Visual inspections of each fire extinguisher should be performed every month using the above points. The inspection should be noted on the back of the annual inspection tag.
Safety Shower and Eyewash
Safety showers should be available in labs where there is the potential hazard of injury to the skin due to contact with a corrosive, severely irritating or toxic chemical. Eye washes should be available in labs where there is the potential hazard of injury to the eye due to contact with a chemical or where they are exposed to biological materials that are Risk Group
2 or greater.
35.
Safety Shower/Eyewash is unobstructed.
Safety showers and eye washes should be in accessible, unobstructed locations that require no more than 10 seconds to reach.
36.
Safety Shower/Eyewash location is identified by a highly visible sign.
Safety shower and eye wash locations should be identified with a highly visible sign that is visible within the area served by the safety shower and/or eye wash.
37.
Safety Shower/Eyewash is tested regularly.
Safety showers should be activated and tested at least annually. Eye washes should be activated and tested weekly.
These equipment inspections should be recorded on tags, initialed and dated after performing the inspection.
Chemical Safety
38.
Spark producing equipment is kept away from flammables.
Spark producing equipment, such as Variacs or Rheostats, should not be located in an area where flammable gases or liquids are stored or used (e.g., laboratory chemical hoods).
39.
No Food & Drink in lab
Food and beverages are forbidden in laboratories. Food and beverages must not be stored in refrigerators that also store biological, chemical or radioactive materials.
40.
House Vacuum – protected from vapors
No liquids should be aspirated directly into the house vacuum lines. If laboratory personnel from different laboratories aspirate incompatible chemicals through the vacuum lines, those chemicals can violently react with each other. Additionally, vacuum lines can become blocked and vacuum pump oil can become contaminated and degraded, resulting in damage to the system.
Laboratory Management Plan (LMP)
41.
Current LMP is available.
A current copy of the laboratory’s LMP should be available to all laboratory personnel.
42.
Lab-specific safety information is included.
The LMP should identify all the hazards present in that laboratory and describe specific measures for effectively controlling those hazards. The laboratory’s LMP is usually presented as general safety information (refer to the USM
Laboratory Safety Handbook and supplemented by laboratory-specific safety information.
43.
Lab personnel are trained on the Laboratory Management Plan
44.
LMP
All laboratory personnel should know the location of the LMP and be familiar with its contents.
45.
Material Safety Data Sheets are available.
Laboratory personnel should know how to obtain a Material Safety Data Sheet (MSDS) for any given chemical in the laboratory (this is a required part of the LMP). MSDSs should accompany all purchased chemicals.. Assistance with obtaining MSDSs can be found by contacting EHS.
46.
Chemical inventory available
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The University of Southern Mississippi requires all laboratories with chemicals to have the inventories in the Chem
Tracker database. (Note: Chemicals may not be purchased unless the lab is registered in Chem Tracker) Without a chemical inventory, it is difficult to support the claim that all the hazards in a laboratory have been identified. A chemical inventory helps keep chemical quantities low and helps prevent over-purchasing. In addition, inventories can sometimes be useful in responding to an incident in the lab.
Personal Protective Equipment
47.
Eye/face protection is worn while in lab.
Anyone in a laboratory should be wearing appropriate eye/face protection. At a minimum, this means safety glasses with side shields (ANSI Z-87-1). Chemical goggles are recommended if there is a splash/spray hazard.
48.
Lab coats or aprons are worn.
Laboratory coats or aprons should be worn to prevent chemical, radioactive or biological materials from coming into contact with the skin or clothing. Any protective clothing worn should never be taken home to wash. Each department should have a laundry facility on site to clean/decontaminate any protective clothing that cannot be discarded. In the case where a department does not have a laundry facility, arrangements should be made with another department or a professional launderer.
49.
Appropriate gloves are worn.
For use with chemicals: Various types of gloves are required for various chemicals. Latex gloves are not a good choice for all-purpose chemical protection. Nitrile gloves are adequate for many chemicals, but the laboratory should have a glove chart for quick reference. If unsure which gloves to use, consult a glove chart such as the one can be found in the resource section of the EHS web site. Disposable gloves should not be reused. Reusable gloves should be checked routinely for holes/leaks.
For use with biological materials: Single-use disposable laboratory gloves are generally adequate. Because of potential allergic reactions, alternatives to latex gloves should be provided. Nitrile gloves are recommended. If reusable gloves are used, they must be decontaminated after each use.
50.
Respirator wearers have received approval, training and fit-testing.
If personnel use respirators, they are required by law to be medically evaluated, fit tested and trained prior to use.
Contact the EHS Department for more information.
Chemical Storage
51.
Chemicals are stored per compatibility.
Incompatible chemicals give an undesired chemical reaction when mixed. This usually refers to substances that will react to cause an imminent threat to health and safety through an explosion, fire, and/or formation of toxic materials.
Generally speaking, segregate acids and bases; acids from cyanides and sulfides; flammables from strong corrosives and oxidizers. For more information refer to the MSDS's for the chemicals.
52.
Chemicals are stored in cabinets or on stable shelving.
Chemicals should be stored in cabinets or on stable shelving. Chemicals should not be stored on the floor or precariously on shelves where they could be knocked off or fall off.
53.
Chemicals are stored in proper containers.
Container material must be appropriate for the chemical contents. Hydrofluoric acid should never be stored in glass.
Nitric acid and other oxidizers should only be stored in glass. Food containers are never to be used for chemical storage, even if the label has been removed.
Chemical Containers
54.
Containers are in good condition.
All chemical containers should be in good condition with no cracks or leaks, and with the appropriate lid/cap. Any container that is not in good condition should be replaced immediately.
55.
Containers are properly labeled.
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The University of Southern Mississippi Laboratory Management Plan
All containers of chemical, biological, and radioactive materials must be labeled as to the contents and its hazard category (refer to a chemical compatibility chart). Even temporary containers should be labeled so that if an emergency arises, another person can identify what is in the container. For chemical waste, the container should describe the contents with the word “waste” (e.g., “waste acetone,” “waste halogenated solvents,” etc.).
56.
Containers are closed when not in use.
All chemicals and chemical waste should be stored in containers that are able to be closed. Chemical containers
(including chemical waste containers) are to be closed at all times unless in immediate use. Immediate use means that a person is in the vicinity of the container and is actively adding or removing chemicals from the container.
57.
Secondary containment is used.
If bottles must be stored on the floor, they must be in a secondary container (all waste containers must be stored in secondary containment) such as a sturdy plastic tub that minimizes accidental breakage. The secondary container should be able to contain 1.5 times the bottle volume if breakage should occur.
Gas Cylinders
58.
Gas Cylinders are securely fastened to prevent tipping.
Gas cylinders should be secured to a wall or bench, or chained to a cart, to prevent tipping.
Peroxidizable Chemicals
59.
Peroxidizable chemicals are dated when opened.
Peroxidizable chemicals react with air to form shock-sensitive, explosive peroxides. They must be dated when opened.
60.
Peroxidizable chemicals are tested for peroxides every six months
Once opened, peroxidizable chemicals should be tested every six months for the presence of peroxides. Peroxidizable chemicals should be disposed of if no longer needed or if they have formed peroxides. For more information on peroxidizable chemicals see Safety in Academic Chemistry laboratories or contact the EHS Department
Flammable Liquid Storage
61.
Refrigerator is explosion-proof/-protected.
Flammable materials that must be kept in a refrigerator must be stored in one designed or modified for flammable storage or one that is explosion-proof. Standard household refrigerators have exposd ignition sources, making the refrigerator unsuitable for flammable material storage.
62.
Flammable cabinets are used.
If large amounts (gallon quantities) of flammable or combustible liquid are kept in a laboratory, flammable cabinets may be required. Consult with EHS and Fire Safety for more information.
63.
NFPA maximum allowable storage volumes are not exceeded.
The National Fire Protection Association (NFPA) has set limits on flammable liquids stored in laboratories. Maximum quantities for flammable liquid storage are determined based on the type of laboratory inspected, the hazard classification of the flammable liquid, the container used for storage, and the fire protection features of the laboratory.
Mercury Devices
64.
Contact the EHS for details on how to reduce the amount of mercury present in the laboratory.
65.
No mercury thermometers should be used in ovens. Mercury thermometers should not be used in heated ovens.
Broken thermometers in ovens pose a health hazard because heated mercury will volatilize such that it can be breathed in. This will also contaminate the oven, requiring its removal and disposal. Disposing of mercurycontaminated items is very expensive.
Chemical Fume Hoods
66.
Laboratories should arrange to have their fume hoods inspected annually. Any fume hood found not in proper operating condition will be reported to Physical Plant. The PI or instructor of the laboratory where a non-
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The University of Southern Mississippi Laboratory Management Plan operational hood is located will be notified. That individual should also report the discrepancy to the Physical Plant. At any time a malfunctioning hood is identified it should be immediately reported to Physical Plant.
67.
Air flow into the hood is unobstructed.
Excess chemicals and/or equipment should not be stored in the hood, especially if it blocks proper air flow (i.e., blocks the slots between the back baffles). Large items that must be in a hood should be elevated approximately two inches on blocks or on a stand with legs to allow air flow beneath the item into the back bottom slot of the hood interior.
Biological Safety
1.
Functional sink is in laboratory space.
Laboratories should have a sink for handwashing. The sink should be kept stocked with soap and paper towels. A handwashing policy should be communicated to all laboratory members, which directs staff and students to wash their hands after they handle viable materials, after removing gloves, and before leaving the laboratory.
2.
Exterior windows are sealed shut, or have fly screens present.
If installing screens is not an option, windows should be sealed shut.
3.
Biological Safety Cabinet(s) have been certified within the last year.
A sticker that lists the last certification date should be present on the cabinet. It is required that a BSC be certified at the time of installation, annually thereafter, and any time the unit is relocated.
4.
Lab space can be easily decontaminated.
Spaces between benches, cabinets, and equipment should be readily accessible for cleaning. Carpets and rugs are prohibited because they are difficult to decontaminate.
5.
Appropriate disinfectant for material is in lab.
Work surfaces should be decontaminated on completion of work, at the end of the day, and after any spill or splash of viable material with disinfectants that are effective against the agents of concern. For most organisms, a 10% bleach solution is effective. However, for some organisms, 70% ethanol may be required. Note that bleach solutions should be prepared fresh each day.
Bench Tops
6.
Bench tops are made of an impervious material.
Self-explanatory (to allow for easy decontamination).
7.
Bench tops are resistant to chemicals and moderate heat.
Bench tops should be resistant to acids, alkalis, organic solvents and moderate heat, any of which may contact the bench top during normal usage or during decontamination.
Laboratory Furniture
8.
Furniture is appropriately sturdy.
Laboratory furniture should be sturdy, in good condition, and capable of supporting anticipated loading and uses.
9.
Furniture can be easily decontaminated.
Laboratory furniture should be easy to decontaminate. Accordingly, cloth-covered chairs are prohibited. Vinylcovered chairs are acceptable.
Biohazard Waste Containers
10.
Containers are able to be closed.
All containers and bags used for waste collection should be able to be closed. If a waste collection bag is kept in a container, the container should have a lid.
11.
Containers are closed when not in use.
When not in use, biohazard waste containers should be kept closed.
12.
Containers display the biohazard symbol.
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All containers and bags used for waste collection should prominently display the international biohazard symbol. All bags used for waste collection must have the biohazard symbol printed on the bag. If the bag is kept in a container, the container should also have the biohazard symbol prominently displayed.
13.
Containers are appropriate for transport to autoclave.
Durable, leak-proof containers should be available to transport waste to the autoclave for decontamination.
Secondary containment for autoclave bags helps prevent spills of material from unexpected holes or tears in the bag.
Appropriate containers for transport include plastic or metal tubs. Do not place transport containers in the autoclave unless you are certain they are composed of “autoclavable” material. If bags are heavy, use a cart for transporting.
14.
Sharps Disposal Containers are present.
Sharps disposal containers should be present for the proper disposal of laboratory sharps. Sharps Disposal
Containers are available from the science storeroom and come in various sizes. EHS provides pick-up of full containers for disposal.
Radiation Safety
1.
Weekly contamination surveys are conducted.
Laboratories must perform and document surveys for radioactive contamination at least monthly and whenever quantities exceed thresholds specified in the laboratory’s Radiation Permit.
2.
All areas/containers with radioactive materials are identified with signs/labels.
3.
Equipment for detecting radiation hazards is readily available.
4.
Material is secured when unattended.
Radioactive material must be attended by trained personnel or secured from removal when not attended.
Radioactive Materials Recordkeeping
Laboratories must maintain records of the radioactive materials they possess and use, including records of liquid wastes disposed through the sanitary sewer.
5.
Inventory of the lab’s radioisotopes is available.
6.
Records of quantities used are available.
7.
Records of liquid waste disposed of via the sanitary sewer are available.
Radioactive Waste Containers
Waste must be collected in appropriate receptacles and segregated according to half-life. Waste receptacles must be properly labeled and the contents of each waste parcel must be recorded.
8.
Waste container is of appropriate material.
9.
Wastes are segregated per half-life.
10.
Waste container is properly labeled.
11.
Contents of the waste container are recorded.
Containment and Shielding
12.
Workspace is lined with absorbent paper.
Benches, fume hoods, etc., where loose radioactive materials are used must be lined with absorbent paper.
13.
Shielding is used for highly radioactive materials.
Sources with high levels of external exposure should be used and stored behind shielding to minimize exposures.
14.
Tools are used for highly radioactive materials.
Sources with high levels of external exposure should be handled with appropriate tools to minimize exposures.
Lasers
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The University of Southern Mississippi Laboratory Management Plan
15.
Class IIIB or IV lasers are registered.
Section 10 – Incidents, Injuries, and Corrective Actions
Include in this section a description of laboratory incidents and corrective actions taken to prevent them in the future. It is always important to take a careful look for all underlying and precipitating causes. Copies of any incident-related documents or forms should be maintained here.
Please note: An Injury or Illness Report Form must be completed for all workplace injuries and illnesses .
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The University of Southern Mississippi Laboratory Management Plan
Section 11 – Chemical Waste Requirements
Persons who generate, manage or request pickups for chemical wastes must be appropriately trained in the chemical hazards of the waste, acceptable waste containers, and proper waste container labeling. List the persons who have received the training and record their training in this section:
Name: Training Date:
You can record the specific wastes your lab generates (Types and expected quantities). Each laboratory has knowledge of their specific types of wastes. EHS requires chemical wastes to be accurately and properly identified and the containers to be labeled with the concentration and contents. Each department is responsible for providing appropriate labeling materials..
For information on waste pick-ups contact the EHS Department .
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The University of Southern Mississippi Laboratory Management Plan
Section 12 – Radiological Hazards
Various types of records are required for regulatory compliance in all laboratories using radioactive materials.
Copies of these forms and other documents should be maintained in this section. If maintained in the Radiation
Safety Office, include location of the office. The radiation safety office is located in Rm 241 WSB
If applicable please complete
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The University of Southern Mississippi Laboratory Management Plan
Section 13 – Biological Hazards
Biological hazards are subject to additional regulatory requirements, including recordkeeping. Various types of records, such as a list of potentially exposed personnel, any procedures involving biohazardous materials or waste, and other documents should be maintained in this section, or referenced otherwise. If there is any question concerning the use of Select Agents, Prior to use, clearance must be documented and signed by the
Bio-Safety Officer.
If there are any questions they should be directed to the Bio-Safety Officer in EHS. If laboratory procedures involve the use of human derived products covered under the Bloodborne Pathogen
Standard, please reference the Exposure Control Plan here.
If applicable please complete
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The University of Southern Mississippi Laboratory Management Plan
Section 14 – Laboratory Animals
Copies of procedures for handling laboratory animals, and other documentation pertaining to human and animal safety and regulatory compliance, should be maintained in this section. For additional information contact DSS.
If applicable please complete
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The University of Southern Mississippi Laboratory Management Plan
Section 15 – Safety Program Correspondence
Copies of correspondence to Principal Investigators from DSS, safety-related memos within laboratory groups, requests for safety information, and other correspondence that may be important to safety management should be maintained in this section.
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The University of Southern Mississippi Laboratory Management Plan
Section 16 – Personal Protective Equipment (PPE) Assessment
Date: Job Title:
Department:
Location:
Employee Name(s):
Tasks
Supervisor:
Analysis By:
Signature:
Potential Hazard PPE Recommended
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The University of Southern Mississippi Laboratory Management Plan
Section 17 – Hazard Assessment Information and PPE Selection Information
Job Title
Department
Student/Laboratory Worker Date
All Supervisor
Current
Location Research/Teaching Labs Analysis by
Employee Name(s) All Signature
Tasks Potential Hazard PPE Recommended
Working with small volumes of corrosive liquids < 1 liter
Skin and eye damage
Safety glasses, goggles (if splash hazard), Light chemical resistant gloves, Lab coat, closed shoe
Working with large volumes of corrosive liquids > 1 liter, acutely toxic corrosives or work which may create a splash hazard
Large surface area skin and eye damage, poisoning, or great potential for eye and skin damage.
Safety goggles & face shield
Appropriate heavy resistant gloves
Above clothes and chemical resistant apron
Working with small volumes of organic solvents < 1 liter
Skin and eye damage
Slight poisoning potential through skin absorption
Safety glasses, goggles (if splash hazard), Light chemical resistant gloves, Lab coat, closed shoe
Working with large volumes of organic solvents > 1 liter, very dangerous organic
Major skin and eye damage, poisoning through skin
Safety goggles & face shield
Appropriate heavy resistant gloves solvents or work which may absorption
Above clothes and chemical resistant apron create a splash hazard
NOTE: Please reference the specific glove manufacturer’s selection chart for proper selection of all gloves based on the specific hazard.
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The University of Southern Mississippi Laboratory Management Plan
Job Title
Department
Student/Laboratory Worker Date
All Supervisor
Current
Location Research/Teaching Labs Analysis by
Employee Name(s) All Signature
Working with small volumes of human blood, body fluids or other Bloodborne
Pathogens (BBP)
Working with large volumes of human blood, body fluids or other Bloodborne
Pathogens (BBP) and/or splash hazards
Working with hazardous powders
Working with acutely toxic hazardous powders
Working with radioactive materials
Tasks Potential Hazard
Potentially infectious disease
(BBP) Potential spread of infectious disease
Increased potential of becoming infected with infectious disease (BBP)
Increased potential spread of infectious disease
Potential skin and eye damage, Potential for poisoning through skin absorption
Great potential skin and eye damage
Great potential for poisoning through skin absorption
Potential cell damage.
Potential spread of radioactive materials
PPE Recommended
Safety glasses, latex gloves
Lab coat, closed shoe
Safety goggles & face shield
Latex gloves
Lab coat, closed shoe coveralls and foot covers may be necessary
Safety glasses
Goggles for large quantities
Chemical resistant gloves
Lab coat, closed shoe
Safety goggles
Appropriate heavy resistant gloves
Lab coat, closed shoe
Coveralls and booties if necessary
Safety glasses, goggles splash hazard
Latex gloves
Lab coat, closed shoe
Working with radioactive chemicals (corrosives, solvents, powders, etc.)
See appropriate chemical section above
Potential cell damage.
Potential spread of radioactive materials
Safety glasses, goggles splash hazard
Chemical resistant gloves
Lab coat, closed shoe
Use PPE for applicable tasks above
Potential cell damage
Working with radioactive human blood, body fluids or other BBPs
Potential spread of radioactive materials
Potential BBP exposure
Safety glasses, goggles splash hazard
Latex gloves
Lab coat, closed shoe
NOTE: Please reference the specific glove manufacturer’s selection chart for proper selection of all gloves based on the specific hazard.
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The University of Southern Mississippi Laboratory Management Plan
Job Title
Department
Student/Laboratory Worker Date
All Supervisor
Current
Location Research/Teaching Labs Analysis by
Employee Name(s) All Signature
Tasks Potential Hazard
Working with cryogenic liquids
Working with very cold materials and equipment
(freezers, dry ice)
Major skin, tissue and eye damage
Skin damage
PPE Recommended
Safety glasses or goggles for large volumes or splash hazards
Heavy insulated gloves
Lab coat, closed shoe
Safety glasses
Insulated gloves
Lab coat, closed shoe
Working in cold environments (walk-in cold rooms or freezers)
Working with hot liquids, equipment and/or open flames (autoclave, Bunsen burner, waterbath, oil bath)
Working with large volumes of hot, cold, or cryogenic liquids
Frostbite (skin damage)
Hypothermia
Skin damage
Eye damage
Major skin and eye damage
Frozen or burned body tissues
Safety glasses
Insulated gloves and warm clothing
Lab coat, closed shoe
Safety glasses or goggles for large volumes or splash hazards
Insulated gloves
Lab coat, closed shoe
Safety glasses or goggles and face shield
Heavy insulated gloves
Above clothes and apron or coveralls
Working with Ultraviolet
Radiation
Conjunctivitis
Corneal eye damage
Erythema
UV face shield and goggles
Lab coat, closed shoe
Appropriate shaded goggles with optical density based on individual
Working with LASER radiation
Retinal eye damage
Skin damage
No jewelry/reflective items allowed
NOTE: Please reference the specific glove manufacturer’s selection chart for proper selection of all gloves based on the specific hazard.
beam parameters
Lab coat, closed shoe
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The University of Southern Mississippi Laboratory Management Plan
Section 18 – Laboratory Safety Handbook
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The University of Southern Mississippi Laboratory
Management Plan
USM
Department of EHS
Walker Science Building Room 240
March, 2014
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The University of Southern Mississippi Laboratory
Management Plan
I. INTRODUCTION ................................... 46
II. PUBLICATIONS ................................... 47
III. COMMITTEES .................................... 48
IV. LABORATORY SAFETY SURVEYS 49
V. USM LABORATORY SAFETY .......... 50
VI. CHEMICAL HAZARD CLASSES ..... 53
VII. TYPES OF EXPOSURES AND ......... 54
VIII. PARTICULARLY HAZARDOUS ... 55
IX. STANDARD SAFETY ........................ 56
X. CHEMICAL HAZARD ......................... 57
XI. CHEMICAL PROCUREMENT .......... 58
XII. CHEMICAL STORAGE .................... 59
XIII. PRIOR APPROVAL ......................... 60
XIV. SAFETY AND EMERGENCY ........ 61
XV. PERSONAL PROTECTIVE .............. 62
XVI. FUME HOODS ................................. 63
XVII. BIOLOGICAL SAFETY ................. 64
XVIII. MEDICAL CONSULTATION ...... 65
XIX. WASTE SEGREGATION AND ....... 66
XX. COMPRESSED GASES AND ........... 68
XXI. ELECTRICAL SAFETY................... 69
XXII. BIOLOGICAL SAFETY ................. 70
XXIII. ELECTROMAGNETIC ................. 71
XXIV. Chemical Incompatibility Chart ..... 73
TELEPHONE NUMBERS ...................... 74
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The University of Southern Mississippi Laboratory
Management Plan
In January, 1990, the Occupational Safety and
Health
Administration (OSHA) enacted the regulation entitled Occupational Exposure to Hazardous
Chemicals in Laboratories (29CFR1910.1450 - commonly called "The Lab Standard"). This standard deals specifically with hazardous chemical recognition, safe use, storage, and disposal.
There are many hazards present in laboratories besides hazardous chemicals. Non-chemical lab hazards include compressed gases, cryogenic fluids, biohazards, electrical hazards, mechanical hazards, radiation, and radioisotopes. Non-chemical hazards are discussed in the latter part of this publication.
This handbook provides basic information for many important topics concerning laboratory safety. It is neither a complete nor comprehensive presentation of lab safety. Additional information may be obtained by calling the Department of EHS at 266-
6912.
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The University of Southern Mississippi Laboratory
Management Plan
The Department of EHS has produced several publications that are directly or indirectly related to lab safety. A description of each publication follows.
Laboratory Management Plan - To be proposed to respond to the OSHA Lab Standard involving hazardous chemicals.
Waste Disposal Guidelines - Information concerning retention, treatment, and acceptable disposal of chemical waste is provided in this publication. Each department will be provided with a copy of these guidelines.
Bloodborne Pathogens-Exposure Control Plan - This publication will respond to the OSHA Regulation involving exposure to human blood and body fluids.
Each department will be provided with a copy of these guidelines.
Biosafety Manual - This publication contains guidelines for the safe use of biological agents in the laboratory. Areas covered include biological agent summaries and classification, biowaste decontamination and disposal, spill response, bio-afety cabinets and biohazard shipments.
Emergency Procedures Handbook - This publication provides actions to take and telephone numbers to call in case of emergencies such as fire, chemical spill, or injury. Presented in a handy flip-chart format, these are posted inside each laboratory.
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The University of Southern Mississippi Laboratory
Management Plan
The University has organized committees to deal with safety issues relative to government regulations and guidelines, and prudent practices.
Following are the committees that are involved with laboratory safety issues.
Laboratory Safety Committee - A campus-wide committee that is responsible for all laboratory safety issues not covered by other specific committees. This committee formally reports to the
EHS Department for action on policy recommendations.
Institutional Biosafety Committee - This committee is responsible for review and approval of research activities and proposals involving recombinant DNA according to federal guidelines.
Biohazard Committee - All issues and research studies that involve non-recombinant DNA biological agents are reviewed by this committee.
Animal Care and Use Committee - The College of Science and Technology has a committee to review and approve activities and proposals for experimental animal use in compliance with federal regulations.
Radiation Safety Committee - The radiation safety committee is responsible for review and approval of radiation source and use activities in compliance with federal regulations.
Departmental Laboratory Safety Coordinators -
Although not a formal committee, each department with laboratories has appointed a safety coordinator.
This person is responsible for providing appropriate safety information to staff in his department and coordinating safety improvements recommended for the department.
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The University of Southern Mississippi Laboratory
Management Plan
The department of EHS conducts inspections of all campus labs on an annual or more frequent basis.
Results of the inspections are provided to the department in writing, usually through the department lab safety coordinator. The following general categories are examined relative to required or recommended practices.

Emergency Procedures Posting

Emergency Equipment - eyewash, shower, etc.

Fume Hood/Biological Safety Cabinet

Personal Protective Equipment

Housekeeping and Hygiene

Chemical/Biological Storage

Chemical and Biohazardous Waste

Labeling

Electrical

Compressed Gas/Cryogenic Liquids

Equipment Guarding

Lab User Knowledge of Safety Materials

Fire Safety

Hazard/Safety Signage
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The University of Southern Mississippi Laboratory
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Policy on Eye and Face Protection in Laboratories
Eye and face protection shall be used according to the laboratory classification system. Each department shall determine the hazard class of each laboratory. These requirements should be posted outside each laboratory door. Protective equipment will be provided to employees at no charge. Each department will be responsible for enforcement of this approved policy. If the recommended policy does not apply to a particular situation, departments must provide an alternative policy for approval by the Laboratory Safety Committee. These requirements apply to all persons entering the laboratory. If a procedure creates a greater hazard than the laboratory classification would indicate, eye and face protection appropriate for the hazard shall be worn. Guidance for the selection of eye and face protection is given in the "American National
Standard for Occupational and Educational Eye and
Face Protection" (ANSI Z87.1). EHS will assist in determining the appropriate eye and face protection for specific laboratory hazards and will provide vendor information for securing equipment.
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The University of Southern Mississippi Laboratory
Management Plan
Laboratory Classification System
CLASS 1 - EYE/FACE PROTECTION NOT
REQUIRED
Laboratories that do not use chemicals, biologicals or physically hazardous materials.
Example: computer laboratory
CLASS 2 - EYE/FACE PROTECTION
REQUIRED
WHEN HAZARD EXISTS
Laboratories that use chemicals, biological or physically hazardous materials on an occasional basis.
Example: laser laboratory, some biological labs
CLASS 3 - EYE/FACE PROTECTION
REQUIRED
AT ALL TIMES
Laboratories that routinely use chemicals, biologicals, or machinery
Example: most research laboratories
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The University of Southern Mississippi Laboratory
Management Plan
Policy on Eating, Drinking, and Related
Activities in Laboratories
Hazardous materials can be accidentally ingested when eating, drinking, gum chewing, or related activities are not permitted within workplace and teaching laboratories. To eliminate this potential route of exposure, OSHA has developed guidelines which prohibit these activities in areas where laboratory chemicals are present. In addition,
OSHA recommends hand washing before these activities are conducted.
Eating, drinking, gum chewing, the application of cosmetics, and the storage of food and beverages are not permitted in laboratories containing hazardous materials. These activities may take place in a separate area which is a room with floor to ceiling walls and a door separating the area from the laboratory space in which hazardous materials are used, stored, or transported.
Chemical/Spill Reporting Policy
All chemical spills should be reported to EHS immediately.
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The University of Southern Mississippi Laboratory
Management Plan
Laboratory chemicals may be catagorized according to the type of hazard that they present. Following are major hazard classes and examples of chemicals belonging to each class.
Toxic - Materials causing immediate (acute) or longterm/delayed (chronic) health effects. Exposure limits for many chemicals have been established according to the toxicity of the material. Examples: f ormaldehyde, methylene chloride, phenol.
Flammable - Material that can burn or explode when exposed to heat, sparks or open flames.
Examples: ethanol, hexane, xylene.
Oxidizers - Materials that provide oxygen or other oxidizing elements that can cause fires in contact with flammables or can cause significant burns/ irritation to the skin. Examples: hydrogen peroxide, perchloric acid, potassium dichromate.
Corrosive - Substances that can damage exposed body tissue, including acids and bases. Examples: acids - hydrochloric, sulfuric; bases - sodium hydroxide, ammonia (ammonium hydroxide).
Peroxide formers - Chemicals that produce explosive peroxides when concentrated or exposed to oxygen (air). Examples: ethyl ether, dioxane,
THF.
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Chemicals can cause illness according to the type of exposure experienced. Following are the routes of entry for chemical exposure.
♦ Inhalation
♦ Ingestion
♦ Absorption (skin or eye contact)
♦ Injection
Prevention of illness caused by chemical exposure relies on preventing the chemical contacts listed above. One or more of the following control measures should be employed when hazardous chemicals are involved in any lab procedure.
♦ Engineering - substitution, isolation, enclosure, ventilation.
♦
Administrative - scheduling, training, medical monitoring.
♦ Personal Protective Equipment (PPE) - gloves, safety glasses, lab coats, face shields.
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VIII. PARTICULARLY HAZARDOUS
SUBSTANCES
Although care must be taken with all chemicals, certain chemical classes require special attention due to their potential to cause significant health effects.
Specific precautions must be taken according to the
Standard Safety Operating Procedures (SSOPs) developed for the chemical to be used. SSOPs should be developed by the department or lab using the chemical in conjunction with EHS.
Types

Carcinogens – Materials known to cause cancer

Reproductive Toxins - Mutagens (cause genetic mutations) and teratogens (cause birth defects)

Acute Toxics (HMIS rated 3 or 4)

Chemicals of Unknown Toxicity
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The OSHA Laboratory Standard, which requires the preparation of a Chemical Hygiene Plan (USM
LMP) also requires written standard safety operating procedures (SSOPs) to be followed when laboratory work involves the use of hazardous chemicals. A separate SSOP must be completed for every hazardous material and procedure that has characteristics different from other materials and procedures used. Hazardous materials with the same safety procedures may be grouped together in the same SSOP. Written SSOPs should be kept in an accessible location with other safety information
(such as MSDSs) so that lab users can check safety procedures prior to their use of a new chemical or procedure. The following are key issues to consider for preparing SSOPs:
♦ Personal protective equipment (eye, face, hand protection, etc.)
♦ Procedures to minimize chemical exposure
(splashing and aerosol minimization)
♦ Location for use (designated area such a fume hood)
♦ Labeling information
♦ Methods of measuring and transferring materials (weighing and dispensing equipment)
♦ Decontamination procedures (materials used for routine or spill cleanups)
♦ Locations for storage (flammable, refrigerated, or other storage requirements)
♦
Waste disposal (containers, labeling, segregation, and storage)
♦ Transportation of hazardous materials outside of the lab
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Determining the hazards presented by chemicals in use is the first step in preventing adverse health effects. The following information resources will provide the type and relative severity of chemical hazards and control measures to prevent overexposure.
♦ Container labels
♦ Chem Tracker database and the internet
♦ Material Safety Data Sheets (MSDSs)
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When purchasing chemicals for lab use, there are several considerations that should be taken.

Investigate the use of less hazardous materials.

Plan for containment, storage, disposal, and personal protective equipment.

Order appropriate (minimal) amount - limit storage space and disposal costs.

Date chemicals when received and opened.

Insure that all manufacturer's chemical bottles and secondary containers are properly labeled.
Note: All procured chemicals are received in the chemical storeroom. A Chem Tracker barcode is affixed to the container. When the container is empty, the barcode must be removed and returned to EHS.
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Proper storage of chemicals helps eliminate breakage, chemical spills and inadvertent mixing of noncompatible materials. The following are precautions that should be taken.

Store chemicals by hazard class and segregate each class in a separate area. A
Chemical Incompatibility Chart is provided in this document. A list of the minimum number and type of hazard classes follows.

Flammables
 Acids
 Bases
 Oxidizers

No hazard

Chemical bottles should not be stored on the floor or above eye level.
 Store over 10 gallons of flammable materials in an approved flammable storage cabinet.
 Only chemicals in current use should be on the lab bench or in the fume hood.
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Certain activities may create special hazardous situations and require prior approval. The OSHA
Lab Standard requires each employer to assess activities and determine which will require prior approval. Prior approval policies and procedures are initiated by each department according to their needs. Examples of activities that may require prior approval follow.

Sole occupancy of a laboratory or building

Hazardous operations, equipment and/or chemicals

New procedures or new chemical use

Unattended operations
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In order to be prepared before an emergency incident occurs, knowledge of safety equipment use/location, and appropriate room layout are necessary. All lab workers should be aware of the following for the lab that they work in.
Know:

Location of Emergency Procedures
Handbook.

Location of nearest eyewash and shower.

Location and use (if trained) of fire extinguisher.

Location of nearest fire alarm pull station.

Two ways to exit the building.
Avoid:

Blocking emergency equipment - fire extinguishers, emergency showers, eyewashes, and electrical panels.

Storing materials on floors, in aisles, and in exits.
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Personal protective equipment includes clothing and devices that are worn by the lab worker to protect him/her from hazards. Following are PPE equipment categories and recommendations.
Eye protection - In addition to the safety glasses requirements stated in the USM Policy, goggles (and a face shield in certain cases) are required for the use of liquids that could damage eyes if splashing occurs. Eye protection equipment should be stored in its original container or a sealed plastic bag.
Gloves - Gloves appropriate for the material handled should be worn when there is potential for chemical exposure, injury or irritation (see Section
MSDS for the material being used for glove selection criteria).
Body protection - Street clothing should be covered with a lab coat or other protective clothing when working with chemicals in the lab.
Waterproof clothing or a rubber apron may be needed to prevent soak through or caustic burns.
Shorts and sandals should not be worn in labs. Long hair and baggy clothing should be properly confined.
Note: PPE should remain in the lab when exiting.
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1910.1450(e)(3)(iii)
A requirement that fume hoods and other protective equipment are functioning properly and specific measures that shall be taken to ensure proper and adequate performance of such equipment;
Chemical fume hoods should tested annually for acceptable airflow. Following are recommendations to help maintain a safe atmosphere for the fume hood operator.
Confirm airflow - Use a hood monitor, flow indicator, or strip of tissue taped to the bottom of the hood sash for airflow confirmation.
Sash position - Maintain bottom of hood sash at indicated level (arrows) or lower, as shown on the hood certification sticker.
Work position - Position equipment and chemicals at least six inches inside the face of the hood or sash.
Avoid - Blocking face or back baffle with large equipment (elevate equipment about two inches) and using hood as a chemical storage area.
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Biological safety cabinets (also known as laminar flow hoods) are different than fume hoods in that they capture microbiological materials using a High
Efficiency Particulate Air (HEPA) filter. Most
BSCs are not vented to the outside of the building.
Recommendations for the safe use of BSCs follow.
Flammable material use - This is usually prohibited because of the potential for an explosive mixture to develop due to the recirculation of air within the cabinet.
Volatile toxic material use - Gases are not removed by the HEPA filter and are therefore released to the lab unless the BSC is vented to the outside of the building.
Air intake grill - Avoid blocking this grill at the front of the cabinet as this can affect product and personnel safety.
UV light - A UV lamp is often used inside BSCs to disinfect the inside surfaces of the cabinet. This type of disinfection should only be done when no one is using the BSC.
Clean benches - These provide HEPA-filtered air from the back of the cabinet, across the work surface, and out the face of the cabinet. Clean benches do not provide personal protection from materials used in them.
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Incidents or conditions that cause a significant health effect require medical attention in person.
For minor exposures with no symptoms, a telephone call to Office of EHS may be adequate followup.
Following are instances when a medical consultation is necessary. Information on how and when to report an incident is also provided.
Cases requiring medical consultation

If a chemical exposure occurs, including 1) skin or eye contact with hazardous materials,
2) exposure during a chemical spill, or 3) health symptoms (such as headache or rash) develop while working with chemicals.

When air sampling indicates exposure above
OSHA's action level or permissible exposure level.
 For any “on the job” injury.
Reporting procedures for medical consultation.

Complete Occupational Illness/Injury Report.

Report to Student Employee Health Service during normal work hours.

Call University of Southern Mississippi security at 6911 for transportation to emergency center for evening and weekend accidents.
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Laboratory waste must be segregated into the waste streams listed below for safe storage and disposal.
Information about each waste stream disposal follows.
Chemical waste

Follow all provisions of the Waste Disposal
Guidelines.

Segregate chemical wastes into separate containers. Certain similar chemical groups, such as flammable solvents, can be consolidated in the same bottle.
 Label waste containers using a “Waste
Chemical Label”. Stock chemical wastes in their original container with original label do not need to be relabeled.

Recap chemical waste containers when not adding waste to them.

Store chemical waste in closed containers.

Store waste containers in an appropriate location prior to pickup.

EHS will pickup chemical wastes in a timely manner.

It is imperative to remove the Chem Tracker barcode and return to EHS prior to disposal of the container
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Biological waste
Segregate biological waste into sharp and nonsharp categories.

Sharp biohazards, including syringes, needles, , must be placed in a “sharps container” (i.e. red plastic container with
“biohazard” symbol).

Non-sharp biohazards including contaminated glass, rigid plastic pipets, and
Petri dishes must be placed in a autoclavable bag marked with the "biohazard" symbol.
Disposal of biological waste may be accomplished in two ways.

Autoclave in department, mark container
“treated,” and dispose as ordinary trash.

Call EHS at 266-6912 for pickup.
Glass
All uncontaminated or broken uncontaminated glass to be disposed should be placed in a cardboard box marked “glass for disposal”. When two-thirds full, the box should be taped shut and placed with ordinary trash.
Radioactive and mixed wastes
All wastes with radioisotope content should be referred to the Radiation Safety Office for disposal.
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Potential hazards encountered with these materials include explosion, overexposure, crushed feet, a broken-off valve, and frostbite. The following are measures that will limit or eliminate injury when working with compressed gases and cryogenic liquids.

Wear safety glasses when working with compressed gas cylinders.

Wear goggles and a face shield when using cryogenic liquids.

Secure gas cylinders in the upright position using appropriate restraints.

Keep valve cover on cylinder except when regulator is attached.

Open main valve slowly.

Leak-test all connections, especially when using hazardous gases.

Use a hand truck to move gas cylinders.

Wear loose-fitting thermal gloves when working with cryogenic liquids.

Insure that cylinders remain labeled during their entire life in the lab.

Store gas cylinders in a non-corrosive environment.
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Following are recommendations for safe use of electrical equipment.

All equipment with substantial current requirements must be grounded.

Outlet expanders (3:1) and grounded-to ungrounded converters (cheater plugs) are not allowed.

Extension cords should have overload breaker protection and be used only as temporary wiring.

Damaged electrical cords must be replaced
(not repaired).

Ground fault circuit interrupts (GFCI) outlets or equipment should be used within 5 feet of water sources.

All sources of harmful electrical energy must be isolated to prevent electrical shock by contact.
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Biohazards are operationally divided into three categories. Recombinant DNA experimentation is reviewed and approved by the Institutional
Biosafety Committee according to National
Institutes of Health (NIH) Guidelines. Human blood and body fluid exposure is controlled by the OSHA
Bloodborne Pathogens (BBP) regulation. Among other requirements, the BBP standard requires an annual training program for preventing transmission of infectious agents when exposed to human blood and body fluids. Precautions for use of all other biohazards are recommended in the CDC/NIH publication Biosafety in Microbiological and
Biomedical Laboratories . Biohazards are divided into biosafety levels 1 to 4, with 4 being the most significant health hazards. Practices for safe use of biological agents at each level are provided in this publication. The Campus Biohazard Committee reviews and approves protocols involving nonrecombinant DNA experimentation.
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Equipment commonly used in laboratories can produce hazardous amounts of electromagnetic radiation. Information on the radiation types, sources, and means of personal protection follow.
Ultraviolet Light (UV)
Depending on the wavelength and intensity, overexposure to ultraviolet light can result in injury to the skin and/or eyes. Several measures can be taken to avoid this.

Enclose or shield the UV source - acrylic plastic material, such as plexiglass, is an effective absorber of UV light.

Protect eyes with UV-rated safety glasses or goggles.

Protect exposed skin with face shields, long sleeves, and long pants.
Lasers
Lasers can result in skin and/or eye injuries depending on the type and intensity of the source.
Laser safety guidelines have been developed by the
American National Standards Institute. Lasers are classified from 1 to 4 according to their power, with
1 being the least powerful and 4 the most powerful.
Safety measures to be followed during laser use are as follows.
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Lasers (cont.)

Completely enclose the laser system.

Use non-reflective and fire resistant background materials.

Provide warning signs at the room entry for
Class 3 and 4 lasers.
 Provide interlock device or “laser in use” light for Class 4 lasers.

Protect eyes with source and wavelength - specific safety glasses/goggles.
X-Ray Radiation
X-Rays and electron beams are produced by laboratory instruments such as diffractometers, gauging systems, and electron microscopes. X-Ray radiation safety services are provided by the
Radiation Safety Office.
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Chemical Incompatibilities
Chemical
Acetic Acid
Acetylene
Incompatibilities
Chromic acid, nitric acid, hydroxyl compounds, ethylene glycol, perchoric acid, peroxides, permanganates
Chlorine, bromine, copper, fluorine, silver, mercury
Water, chlorinated hydrocarbons, carbon dioxide, halogens
Alkali/alkaline earth metals
(e.g. potassium, sodium, powdered magnesium)
Ammonia (anhydrous)
Carbon tetrachloride
Chromic acid, chromium
Mercury, chlorine, iodine, bromine, HF
Sodium, potassium
Chlorine (also bromine)
Acetic acid, maphthalene, glycerol, alcohol, flammable liquids, camphor
Ammonia, acetylene, butadiene, butane, methane, propane, hydrogen, sodium carbide, finely divided metals, turpentine, benzene
Acids Cyanides
Flammable liquids Ammonium nitrate, chromic acid, hydrogen peroxide, nitric acid, halogens
Ammonia (aqueous or anhydrous) Hydrofluoric acid (anhyd)
Hydrogen peroxide Copper, chromium, iron, most metals or their salts, alcohols, organic and combustible matls.
Acetylene, hydrogen, ammonia (aq or anh) Iodine
Mercury
Nitric Acid (concentrated)
Nitrites
Oxygen
Perchloric acid
Peroxides (organic)
Phosphorous Pentoxide
Sulfuric acid
Acetylene, ammonia, fulminic acid
Acetic acid, aniline, chromic acid, hydrogen sulfide, flammables, any heavy metals (brass)
Acids
Oils, grease, flammables, hydrogen
Acetic anhydride, alcohol, paper, wood, grease, oils, bismuth and its alloys
Acids (organic or mineral), friction, heat
Water, halogenated agents
Potassium chlorate, potassium perchlorate potassium permanganate, (similar compounds of light metals such as sodium and lithium
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Department of EHS
Director ......................................................266-6912
Environmental Manager (Hazardous and
Infectious Wastes) .....................................266-6912
Biosafety Officer .......................................266-6912
Radiation Safety Officer.............................266-6912
Other Safety/Health Areas
Radiation Safety Office .............................266-6912
Emergencies
Police ......................................................911or 6911
Fire .........................................................911 or 6911
Emergency Medical Service ................. 911 or 6911
Utilities ......................................................266-4414
MS Poison Control Center .............1-800-222-1222
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