Supplementary Table S1: Primers used in this study
The following primers (purchased from Eurofins MWG Operon) were used for the
cDNA amplification in the RT-PCR experiments.
Primer
Sequence (5‘-3‘)
rnpB fw
AACTATGACTACCCGCCAGCC
rnpB rev
GGCTTTGCCCAAAGCAAATCG
ocp fw
GGATCAACACCCATGTTGAC
ocp rev
ACGGATGCAGTTTGCTGAAG
sodB fw
GACTCCTCTAAGGTGGGAATC
sodB rev
CCCAGACATCCAAGGTTAAG
pgp fw
TTACCTCTAGGGAAATCC
pgp rev
TATCTCTGGTTTCATCCC
glyk fw
GCGAATTCTGCCACACTCAC
glyk rev
GTCCTCGCCAAATCAATCGG
isiA fw
GGGTCAAGCGCGTAAATTTC
isiA rev
CAAATCCCGCCACCAATTAG
ndaF fw
ATTGCGGCAGAAACGGCTGCT
ndaF rev
ATCGGGTTTACGGCTTTGCACT
Gene
rnpB (Ribonuclease P)
Accession
locus tag
nsp30860
ocp (Orange carotin
protein)
nsp46020
sodB (Fe-superoxide
dismutase B)
nsp31650
pglp (Phosphoglycolate
phosphatase)
nsp48780
glyk (Glycerate kinase)
nsp44980
isiA (CP43’ chlorophyllbinding protein; PsbC
homolog)
ndaF (nodularinsynthetase subunit)
nsp37510
nsp42160
Supplementary Table S2: Overview of transcriptome mapping.
Control
0.5 h HL+O2 stress
3 h HL stress
Library size
41,519,905
39,503,192
38,851,231
Mapped Reads
40,577,305
38,780,157
37,828,087
Mapped non-rRNA
28,214,827 (68%)
27,354,006 (69%)
25,567,480 (66%)
Supplementary Table S4: Quantitative evaluation of the dRNAseq analysis.
Number of genes and transcription start points (TSS) associated with an at least 3fold increased read number in cells exposed to combined high light/oxidative stress
(HL+O2) for 0.5 h or to sole high light (HL) stress for 3 h in relation to the control.
Number of TSS
3-fold increase
(total)
gTSS
iTSS
aTSS
nTSS
3 h HL
0.5 h HL+O2
353
473
133
98
92
30
220
81
115
57
Percentage of total
changes
3 h HL
0.5 h HL+O2
51.0
23.1
17.6
8.3
52.1
20.1
15.4
12.4
Supplemental Table S5: Quantitative evaluation of the RNAseq analysis.
Number of genes and transcription start points (TSS) associated with an at least 3fold decreased read number in cells exposed to combined high light/O 2 (HL+O2) for
0.5 h or to sole high light (HL) stress for 3 h in relation to the control.
Number of TSS
3-fold decrease
(total)
gTSS
iTSS
aTSS
nTSS
3 h HL
0.5 h HL+O2
567
340
149
176
161
81
54
133
114
39
Percentage of total
changes
3 h HL
0.5 h HL+O2
36.0
24.4
28.0
11.6
24.8
30.1
33.3
11.8