Supplementary Information (docx 29K)

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Supplemental Figure legends
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Supplemental Figure 1.
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Class IIa HDACs expression in normal plasma cells from healthy donors and patient MM cells
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Gene expression level was analyzed using publicly available gene profiling expression data (GDS
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4968).
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Supplemental Figure 2.
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Overall survival by Kaplan-Meier plots between low and high HDAC4 expression in relapsed MM
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patients treated by bortezomib.
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Overall survival was analyzed using publicly open gene expression profiling data
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(GSE9782) .Comparison of survival between low HDAC4 group and high HDAC4 group (median
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survival : 565d vs 524d, P=0.42). Thick line low HDAC4 group (n=132), Dotted line high HDAC4
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group (n=132).
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Supplemental Figure 3.
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Cytotoxic effect and PERK-ATF4-CHOP pathway expression by TM treatment against MM cell
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lines
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(A) MM.1S and RPMI8226 cells were treated by TM (0-10μg/ml), and cell growth was assessed by
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MTT assay. Data represent mean ± s.d. in quadruplicate. (B, C) MM.1S cells were treated with TM
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(0-10μg/ml) for 24 h (B) or TM (10μg/mL) for 0-12 h (C). Whole cell lysates were immunoblotted
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with indicated antibodies, and α-tubulin was used as loading control.
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Supplemental Figure 4.
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Cytotoxic effect of CFZ in MM.1S and RPMI8226
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MM.1S and RPMI8226 cells were treated with indicated doses of CFZ, and cell growth was
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assessed by MTT assay. Data represent mean ± s.d. in quadruplicate.
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Supplemental Figure 5.
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Cytotoxic effect of TMP269 combined with CFZ against MM cell lines
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MM1R, U266, DOX40, H929, OPM2, KMS11 and ANBL-6.BR cells were treated with indicated dose
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of TMP269 and CFZ for 48 h, and cell growth was assessed by MTT assay. Data represent mean ±
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s.d. in quadruplicate.
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Supplemental Figure 6.
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Cytotoxic effect of TMP269 combined with CFZ against patient MM cells
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Patient MM cells were treated with or without TMP269 (10μM) in the presence or absence of CFZ
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(4nM) for 48 h, and cell growth was assessed by MTT assay. Data represent mean ± s.d. in
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triplicate. * p< 0.05 compared to control.
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Supplemental Figure 7.
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Cytotoxic effect of CFZ in ANBL-6.WT and ANBL-6.BR.
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ANBL-6.WT and ANBL-6.BR cells were treated with indicated doses of CFZ, and cell growth was
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assessed by MTT assay. Data represent mean ± s.d. in quadruplicate.
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