1 SUPPLEMENTARY TABLE 1 FOR THE MS: 2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages. 3 4 Journal: Behavior Genetics 5 6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders 7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1 8 9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade 10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. 11 2 12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg, 13 Denmark. 14 3 Aalborg Zoo, Aalborg, Denmark. 15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, 16 Winterthurerstrasse 190, 8057 Zurich, Switzerland. Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology 17 18 *Corresponding author: 19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University, 20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E- 21 mail tommaso@biology.au.dk. 22 1 1 Supplementary Table I: LA (± SD) at 3, 10 and 17 days of age, under dark and light regimes. 2 LINE A B C 5 6 7 9 10 11 AGE 3 DARK LIGHT 570±322 622±510 745±321 786±413 695±203 415±251 1118±417 965±599 246±200 534±231 581±187 619±364 364±151 335±134 272±124 679±298 764±532 360±234 AGE 10 DARK LIGHT AGE 17 DARK LIGHT 918±531 862±506 745±321 549±400 716±236 450±191 807±359 946±404 357±309 944±664 660±515 737±342 634±271 587±219 500±303 831±391 376±254 463±319 650±362 571±157 590±351 317±139 347±150 296±184 392±297 647±399 331±270 663±370 545±276 563±320 601±389 219±61 296±119 455±355 363±210 411±229 3 4 5 2 1 SUPPLEMENTARY FIGURE 1,2,3 FOR THE MS: 2 Inbreeding affects locomotor activity in Drosophila melanogaster at different ages. 3 4 Journal: Behavior Genetics 5 6 Authors: Tommaso Manenti*1, Cino Pertoldi1-2-3, Neda Nasiri1, Mads Fristrup Schou1, Anders 7 Kjærsgaard1-4, Sandro Cavicchi1 and Volker Loeschcke1 8 9 1 Department of Bioscience, Genetics, Ecology and Evolution, Aarhus University, Ny Munkegade 10 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. 11 2 12 and Environmental Science, Aalborg University, Sohngårdsholmsvej 57, DK-9000 Aalborg, 13 Denmark. 14 3 Aalborg Zoo, Aalborg, Denmark. 15 4 Institute of Evolutionary Biology and Environmental Studies, University of Zurich, 16 Winterthurerstrasse 190, 8057 Zurich, Switzerland. Department of Biotechnology, Chemistry and Environmental Engineering - Section of Biology 17 18 *Corresponding author: 19 Tommaso Manenti, Department of Bioscience, Genetics Ecology and Evolution, Aarhus University, 20 Ny Munkegade 114-116, Buildg.1540, DK-8000 Aarhus C, Denmark. Mobile: +4550720465, E- 21 mail tommaso@biology.au.dk. 22 23 24 3 1 Supplementary Figure 1: Daily locomotor activity (LA) at 3-day old age and during dark (Dark) 2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the 3 other two columns. The error bars show the standard errors. 20 20 Line A 10 5 0 Light Light Dark Line 7 5 10 5 0 Light 20 0 Dark Light 20 Line C Dark 5 10 5 0 Light 10 5 0 Dark Line 11 15 LA 10 Light 20 Line 10 15 LA 15 10 5 0 Dark Line 9 15 LA 10 Light 20 15 LA LA 15 LA 0 Dark 20 Line B 10 5 0 Dark 4 10 5 20 Line 6 15 LA 15 LA LA 15 20 Line 5 0 Dark Light Dark Light 5 4 1 Supplementary Figure 2: Daily locomotor activity (LA) at 10-day old age and during dark (Dark) 2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the 3 other two columns. The error bars show the standard errors. 20 20 Line A 10 5 0 Light Light Dark Line 7 5 10 5 0 Light 20 0 Dark Light 20 Line C Dark 5 10 5 0 Light 10 5 0 Dark Line 11 15 LA 10 Light 20 Line 10 15 LA 15 10 5 0 Dark Line 9 15 LA 10 Light 20 15 LA LA 15 LA 0 Dark 20 Line B 10 5 0 Dark 4 10 5 20 Line 6 15 LA 15 LA LA 15 20 Line 5 0 Dark Light Dark Light 5 5 1 Supplementary Figure 3: Daily locomotor activity (LA) at 17-day old age and during dark (Dark) 2 and light (Light) regimes. Control lines are represented in the first column and inbred lines in the 3 other two columns. The error bars show the standard errors. 4 20 20 Line A 10 5 0 Light Light Dark Line 7 5 10 5 0 Light 20 0 Dark Light 20 Line C Dark 5 10 5 0 Light 10 5 0 Dark Line 11 15 LA 10 Light 20 Line 10 15 LA 15 10 5 0 Dark Line 9 15 LA 10 Light 20 15 LA LA 15 LA 0 Dark 20 Line B 10 5 0 Dark 5 10 5 20 Line 6 15 LA 15 LA LA 15 20 Line 5 0 Dark Light Dark Light 6 7 6