Table S8. Primers used in genotyping, RT-qPCR, and ChIP.
Primers used for genotyping
Primer pair
Sequences (5’-3’)
Method
Note
Mut1L
GATTTCCCCAAATTCGATGC
Genotyping BRM WT
Primers described in
Mut1R
ATCACCCACGGCGCCTATTCCTAAT
allele
[44]
Lba1
TGGTTCACGTAGTGGGCCATCG
Genotyping brm-1
Primers described in
Mut1L
above
mutant allele
[44]
Genotyping GA1 WT
Primers described in
allele
[68]
ga1-3wtF
ga1-3wtR
TTTGGCCCAACACACAAACAAACCT
T
AAGCTTCGAACTCAAGGTTCTA
ga1-3mutF
AATGAGATTCATGTTTGGC
Genotyping ga1-3
ga1-3mutR
TTTCTTCATACCACCTGCGTTC
mutant allele
RGAwtF
CAGCTAAGCATCCGATTTGC
Genotyping RGA WT
Primers described in
RGAwtR
TCACATAGAGAAGTCACATG
allele
[27]
RGAwtF
above
Genotyping rga-28
Lba1
above
mutant allele
RGL1wtF
GGTTCTTGGTGATGGAATCTCGA
Genotyping RGL1 WT Primers described in
RGL1wtR
TTATTCCACACGATTGATTCGCCA
allele
RGL1wtF
RGL1mutRB
RGL2wtF
RGL2wtR
Above
Genotyping rgl1-2
TGATAGTGACCTTAGGCGACTTTTG
AACGC
mutant allele
TGCGGTACCATGAAGAGAGGATACG Genotyping RGL2 WT
allele
GAG
ATCGGTACCGCCGCGACTCAGG
RGL2wtF
Above
Genotyping rgl2-13
Lba1
Above
mutant allele
this work and [68]
this work and [27]
[27]
this work and [27]
Primers described in
[27]
this work and [27]
Primers used for RT-qPCR
qPP2A-F
TATCGGATGACGATTCTTCGTGCAG
qPP2A-R
GCTTGGTCGACTATCGGAATGAGAG
GAPc-F
AGCTGCTACCTACGATG
GAPc-F
CACACGGGAACTGTAAC
GA3ox1-F2
GA3ox1-R2
GA3ox2-qF2
CCATTCACCTCCCACACTCT
GCCAGTGATGGTGAAACCTT
GA3ox2-qR2
GGGTCGAGTCTGTATGG
GA20ox1-qF2
GATCCATCCTCCACTTTAGA
GA20ox1-qR2
GTGTATTCATGAGCGTCTGA
GA20ox2-qF2
AAAACCCGGTGAGAGTTGTG
GA20ox2-qR2
TAAGCCCAGAAGCTCCATGA
TGGTCCGAAGGTTTCAC
PP2A RT-qPCR
GAPc RT-qPCR
GA3ox1 RT-qPCR
GA3ox2 RT-qPCR
GA20ox1 RT-qPCR
GA20ox2 RT-qPCR
Primers described in
[44]
Primers described in
[27]
Primers described in
[20]
Primers described in
[20]
Primers described in
[21]
this work
1
GA2ox1-qF2
TGAGGACGAGAGGTTGTACGA
GA2ox1-qR2
TCCTTTCGAATTGTTGAAGCC
GA2ox2-qF
GGAACACACAGACCCACAGAT
GA2ox2-qR
TGTATCGGCTAAGACCCTGTG
GID1a-qF
GTTTGGTGGGAATGAGAGAACG
GID1a-qR
CTAAACGCCTCACTGTTCTTCC
GID1b-qF
CATCCAGCATGTAATCCCTTTGG
GID1b-qR
CACTTGTGGAAACTGTACACAACC
GID1c-qF
ATGATGATGGATGGGCTGTT
GID1c-qR
TTCGGTCCCTCCAAACATAG
SCL3-qF
SCL3-qR
ATTATGCGATGTTGCAGG
ATTACACCCACACCAGAC
OFP16-qF2
TCTTCCTCCACCACAACCACTC
OFP16-qR2
ATTCTCTTGACCGGGGTTCTGG
EXP5-qF
AGAGAAGTGGTGGGATAAGGTTC
EXP5-qR
AACAAACGACAGACTTTGACCAT
CYS2-qF
AGGTTTTCTGGTGATCGCTGTC
CYS2-qR
TGGATTCGAAATTGCCGTGTCTG
LTP2-qF
GAACGCGCTTATGAGTTGTG
LTP2-qR
CTTTAGCGGCAGATTGAAGG
GA2ox1 RT-qPCR
GA2ox2 RT-qPCR
GID1a RT-qPCR
GID1b RT-qPCR
GID1c RT-qPCR
Primers described in
[21]
this work
Primers described in
[69]
Primers described in
[69]
this work
Primers described in
SCL3 RT-qPCR
[58]
OFP16 RT-qPCR
this work
EXP5 RT-qPCR
this work
CYS2 RT-qPCR
this work
LTP2 RT-qPCR
this work
Primers used for ChIP
GA3ox1A2-F
GA3ox1A2-R
TGAATTTGGATCATGAAGACG
AGCGCAAGAGGCGTATAGAA
3ox1 –C-F
GACAACCCATCATGTGTATGTATC
CATATGTTAAAGCACTTGTTTTGGT
SCL3-900-F2
TAATGCCAAATGGGTTCATTG
SCL3-900-R2
GCTAATGTTGCATTTTCATTTTC
SCL3-5UTR2-F TCTCTGCAAACCCTAGTTCCTC
SCL3-5UTR2-R TGAAGGCCAAAAGCTTGATT
Amplifies region 1490
GA3ox1-d ChIP-PCR
-1268 upstream from
and ChIP-qPCR
ATG
Amplifies region 1490
GA3ox1-p ChIP-1268 upstream from
qPCR
ATG; primers
described in [70]
SCL3-d ChIP-PCR
Amplifies region
1096-892 upstream
and ChIP-qPCR
from ATG;
SCL3-p ChIP-qPCR
PP2A-Prom-F
PP2A-Prom-R
TTCCGATATTTTCTGAGGAAGAAG
GGGCCTGTCAAAGATAAGAACTAA
PP2A ChIP-qPCR
ChIP-2S2u-F
ChIP-2S2u-R
CACATACCACACCTCATCGTG
CCATTTGTCTCTACGCATTTGA
2S2-u ChIP PCR
18S-F
18S-R
ATACGTGCAACAAACCC
CTACCTCCCCGTGTCA
18S rDNA ChIP PCR
Amplifies region 1942 upstream from ATG;
Amplifies region 600 454 upstream from
ATG
Amplifies target
sequence for BRM as
described previously
[12]
Primers described in
[58]
2