Pig-immunohistochemical protocols at Department of Veterinary
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Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. Antigen Animal origin of antigen Identifying animal cell types Antibody host, isotype etc. Poly (p) Mono (m) Work on cryo (c) and or formalin (f) Company & code owner Cytokeratin Human Epithelia Mouse, IgG1 κ m f Dako M3515 Liljegren et al. 2003 1 Soerensen et al. 2005 2 Kvist et al. 2006 3 Hansen et al. 2010 4 COX-2 Mouse Activated macrophages, endothelial cells, osteoblasts Rabbit p f Cayman 160106 Caspase-3 cleaved ? Apoptosis Rabbit p f Chromogranin A Human Rabbit p f CD3 Pig Neuroendocrine cells in the epithelial T lymphocytes Cell Signaling Technology, ASP 175, # 9661 Dako A0430 Johansen et al. 2012 5 Christiansen et al. 2014 (The local inflam. resp. in exp. spon. porc. endoc. vet path in press) Mkupasi et al. 2013 6 Mouse IgG1 m f, (c) Souther Biotech 451001 Human T lymphocytes Rabbit p f Dako A0452 CD4 Pig T-helper lymphocytes Mouse IgG2b m c Serotec MCA1749 CD8 Pig Mouse IgG2a m c CD79αcy Human T-cytotoxic lymphocytes B lymphocytes Mouse IgG1 m f Labor Dr. Glatthaar (008.295/33-25) Dako M7051 Desmin Human Mouse, IgG1 κ m f Dako M0760 eNOS/NOD type III Human Smooth & striated muscle cells. Endothelial cells Kvist et al. 2006 7 Kvist et al. 2006 3 Sikasunge et al. 2009 8 Isling et al. 2010 9 Isling et al. 2011 10 Soerensen et al. 2012 11 Christiansen et al. 2013 12 Iburg et al. 2007 13 Hansen et al. 2013 14 Isling et al. 2011 10 Isling et al. 2011 10 Kvist et al. 2006 7 Kvist et al. 2006 3 Iburg et al. 2007 13 Sikasunge et al. 2009 8 Ilsing et al. 2010 9 Isling et al. 2011 10 Lab IgG1 m f BDtransduction 610296 Sørensen et al. 2013 15 Lab Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. Fibrinogen + fibrinogen fragments D and E human --- Rabbit p f Dako A0080 120 KD protein structure Glial Fibrillary Acidic Protein (GFAP) human Follicular dendritic cells Astrocytes, ependymal cells IgM m f Dako M7157 Rabbit p f Dako Z0334 Iba1 (Ionized calcium binding adaptor molecule 1) ? Macrophage Goat IgG p f Abcam ab5076 IL-8 Sheep Activated neutrophils, endothelial cells, macrophages IgG2a m f Serotec MCA1660 iNOS/NOS type II Mus Macrophage IgG2a p f IL-1α Human Mouse m Bouin BD Transduction 610332 Abcam 624B3F2 IL-1β Human Activated macrophages, neutrophils Goat IgG p f R&D AB-201-NA Ki-67 Human Proliferation marker IgG1 κ m f Dako M7240 Lysozyme Human Macrophages Rabbit p f Dako A0099 Cow Kvist et al. 2006 7 Kvist et al. 2006 16 Soerensen et al. 2012 11 Soerensen et al. 2013 15 Hansen et al. 2010 17 Sikasunge et al. 2009 8 Christiansen et al. 2013 12 Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Soerensen et al. 2012 11 Christiansen et al. 2013 18 Christiansen et al. 2014 (The local inflam. resp. in exp. spon. porc. endoc. vet path in press) Laursen et al. 2014 (vet immunol immunopath in press) ? Kvist et al. 2006 7 Christiansen et al. 2013 18 Christiansen et al. 2014 (The local inflam. resp. in exp. spon. porc. endoc. vet path in press) Lab Sikasunge et al. 2009 8 Isling et al. 2010 9, Hansen et al. 2010 17 Isling et al, 2011 10 Soerensen et al. 2012 11 Hansen et al. 2013 14 Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. Lyve1 Rabbit Small (afferent) lymph vessel endothelium Myeloid cells IgG p f Abcam ab33682 Lab Myeloperoxidase Human rabbit p f Dako A0398 Human Granulocytes, macrophages, monocytes Mouse, IgG1 m f Serotec MCA874G/MAC387 Matrix metalloproteinase 9 Pig (synthetic peptide) Rabbit p f Commercially available? Matrix metalloproteinase 12 Pig (synthetic peptide) Aveolar macrophages, macrophages, type II pnemocytes, bronchiolar ep. bronchial ep., glandular ep. Aveolar macrophages, macrophages, type II pnemocytes Christiansen et al. 2013 12 Soerensen et al. 2005 2 Kvist et al. 2006 7 Kvist et al. 2006 3 Kvist et al. 2006 16 Isling et al. 2010 9, Isling et al. 2011 10 Soerensen et al. 2012 11 Bruun et al. 2012 19 Bruun et al. 2013 20 Calprotectin, L1 antigen Rabbit p f Commercially available? Neurofilament protein Human Neurons (axons) IgG1 m f Dako M0762 S100 Cow Dendritic cells, follicular dendritic cells, Rabbit p f Dako Z0311 Surfactant protein D Pig Clara cells, type II pneumocytes Mouse m c (f) Serotec Surfactant protein B? ? Rabbit p f Surfactant protein C? Human? Murine? Type II pneumocytes, Clara cells Type II pneumocytes Rabbit p f Upstate Biotechnology, Lake Placid, NY, USA Upstate Biotechnology, Lake Placid, NY, USA TNF alpha Human Mouse m Bouin Vimentin ? Activated macrophages Mesenchymal cells IgG1 κ m f Biosource, clone 68B6A3 Dako M0725 Von Willebrand factor Human Endothel cells Rabbit p f Dako A0082 IgG-Fc Pig IgG, plasma cells Goat p f Bethyl lab A100-104A IgA Pig IgA, plasma cells Goat p f Bethyl lab A100-102A Bruun et al. 2012 19 Bruun et al. 2013 20 Sikasunge et al 2009 8 Soerensen et al. 2005 2 Hansen et al. 2010 17 Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Sørensen et al. 2005 21 Sørensen et al. 2005 2 Bruun et al. 2013 20 Cirera et al. 2006 22 Bruun et al. 2013 20 Kvist et al. 2006 7 Lab Sikasunge et al. 2009 8 Jensen et al. 2010 23 Soerensen et al. 2013 15 Christiansen et al. 2014 (The local inflam. resp. in exp. spon. porc. endoc. vet path in press) Isling et al. 2010 9 Isling et al. 2010 9 Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. IgM-mu Pig IgM, plasma cells Goat p f Bethyl lab A100-100A MHC II Human Dendritic cells, antigen presenting cells Dendritic cells, antigen presenting cells Renal tubular epithelium Mouse m f Serotec, MCA1335 Mouse m Bouin VMRD, clone H42A Sheep p f The Binding Site Ltd., UK/PC071 Pig Uromucoid (THP) Human Melan A ? Melanocytes, melanoma cells Mouse m f AbD Serotec, M2-7C10 Melanoma marker ? Melanocytes, melanoma cells Mouse m f Santa Cruz Biotechnology, Santa Cruz, CA, Clone PNL-2 Alpha-1-antitrypsin ? Macrophages, Rabbit p f Cell Marque, Rocklin, CA Kvist et al. 2006 7 Kvist et al. 2006 3 Isling et al. 2010 9 Soerensen et al. 2005 2 Kvist et al 2006 7 Isling et al. 2010 9, 2011 10 Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Grossi et al. 2014 (doi:10.1177/ 0300985814 521637,vet path) Reference List 1. Liljegren,C.H., Aalbaek,B., Nielsen,O.L., & Jensen,H.E. Some new aspects of the pathology, pathogenesis, and aetiology of disseminated lung lesions in slaughter pigs. Apmis 111, 531-538 (2003). 2. Soerensen,C.M. et al. Pulmonary infections in swine induce altered porcine surfactant protein D expression and localization to dendritic cells in bronchial-associated lymphoid tissue. Immunology 115, 526-535 (2005). 3. Kvist,P.H. et al. Biocompatibility of an enzyme-based, electrochemical glucose sensor for short-term implantation in the subcutis. Diabetes Technol. Ther. 8, 546-559 (2006). 4. Hansen,M.S. et al. An Investigation of the Pathology and Pathogens Associated with Porcine Respiratory Disease Complex in Denmark. Journal of Comparative Pathology 143, 120-131 (2010). 5. Johansen,L.K. et al. Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. Prostaglandins & Other Lipid Mediators 97, 103-108 (2012). Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. 6. Mkupasi,E.M., Sikasunge,C.S., Ngowi,H.A., Leifsson,P.S., & Johansen,M.V. Detection of cysteine protease in Taenia solium-induced brain granulomas in naturally infected pigs. Veterinary Parasitology 197, 360-363 (2013). 7. Kvist,P.H. et al. Biocompatibility of electrochemical glucose sensors implanted in the subcutis of pigs. Diabetes Technol. Ther. 8, 463-475 (2006). 8. Sikasunge,C.S., Johansen,M.V., Phiri,I.K., Willingham,A.L., & Leifsson,P.S. The immune response in Taenia solium neurocysticercosis in pigs is associated with astrogliosis, axonal degeneration and altered blood-brain barrier permeability. Veterinary Parasitology 160, 242-250 (2009). 9. Isling,L.K., Aalbaek,B., Schroder,M., & Leifsson,P.S. Pyelonephritis in slaughter pigs and sows: Morphological characterization and aspects of pathogenesis and aetiology. Acta Veterinaria Scandinavica 52, (2010). 10. Isling,L.K., Aalbaek,B., Birck,M.M., Heegaard,P.M.H., & Leifsson,P.S. Host Response to Porcine Strains of Escherichia coli in a Novel Pyelonephritis Model. Journal of Comparative Pathology 144, 257-268 (2011). 11. Soerensen,K.E. et al. The Impact of Staphylococcus Aureus Concentration on the Development of Pulmonary Lesions and Cytokine Expression After Intravenous Inoculation of Pigs. Veterinary Pathology 49, 950-962 (2012). 12. Christiansen,J.G. et al. Embolic Encephalitis in a Porcine Model of Endocarditis. In Vivo 27, 591-597 (2013). 13. Iburg,T., Johansen,M.V., Leifsson,P.S., Willingham,A.L., & Lindberg,R. Hepatic changes in congenital Schistosoma japonicum infections in pigs. Journal of Comparative Pathology 136, 250255 (2007). 14. Hansen,M.S. et al. Detection of Porcine Circovirus Type 2 and Viral Replication by In Situ Hybridization in Primary Lymphoid Organs From Naturally and Experimentally Infected Pigs. Veterinary Pathology 50, 980-988 (2013). 15. Soerensen,K.E. et al. Disseminated Intravascular Coagulation in a Novel Porcine Model of Severe Staphylococcus aureus Sepsis Fulfills Human Clinical Criteria. Journal of Comparative Pathology 149, 463-474 (2013). 16. Kvist,P.H. et al. Evaluation of subcutaneously-implanted glucose sensors for continuous glucose measurements in hyperglycemic pigs. In Vivo 20, 195-203 (2006). 17. Hansen,M.S., Pors,S.E., Bille-Hansen,V., Kjerulff,S.K.J., & Nielsen,O.L. Occurrence and Tissue Distribution of Porcine Circovirus Type 2 Identified by Immunohistochemistry in Danish Finishing Pigs at Slaughter. Journal of Comparative Pathology 142, 109-121 (2010). 18. Christiansen,J.G. et al. Systemic inflammatory response and local cytokine expression in porcine models of endocarditis. Apmis(2013). 19. Bruun,C.S. et al. Expression of matrix metalloproteinase-9 and -12 in porcine lung infections. J. Comp Pathol. 146, 253-257 (2012). Pig-immunohistochemical protocols at Department of Veterinary Disease Biology KU-SUND Latest revision is March 24. 2014 In red is less well characterized protocols, missing information etc. 20. Bruun,C.S. et al. Functional characterization of a porcine emphysema model. Lung 191, 669675 (2013). 21. Soerensen,C.M., Nielsen,O.L., Willis,A., Heegaard,P.M.H., & Holmskov,U. Purification, characterization and immunolocalization of porcine surfactant protein D. Immunology 114, 72-82 (2005). 22. Cirera,S. et al. Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene. Genomics 88, 659-668 (2006). 23. Jensen,H.E. et al. Histologic and bacteriologic findings in valvular endocarditis of slaughterage pigs. Journal of Veterinary Diagnostic Investigation 22, 921-927 (2010).
