María L. Burgos-Garay PhD, M(ASCP)CM 1095 N. Jamestown Rd. Apt. L Decatur, GA 30033 Cell: 610-207-3255, Work: 404-639-4866; [email protected] QUALIFICATIONS: A proven leader with experience managing a laboratory, training personnel, and contributing to research development of the laboratory. Expertise in molecular biology, microbial ecology, and statistics. Effective presentation skills and good scientific writing. Active user of social media –LinkedIn and Scoop.it for networking and dissemination of information. EDUCATION Ph.D. Plant Pathology and Environmental Microbiology, Pennsylvania State University, June, 2013 B.S. Microbiology, Interamerican University of Puerto Rico, May, 2004 RESEARCH AND WORK EXPERIENCE Microbiologist IV, Chenega for CDC/OID/NCEZID (CTR) (2014-Current) Identified bacterial isolates including carbapenem-resistant Enterobacteriaceae (CRE), Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus (VISA, VRSA, MRSA), and vancomycin-resistant Enterococci (VRE) using MALDI-TOF and VITEK2 Performed conventional techniques to identify antibiotic susceptible isolates o Kirby-Bauer, broth microdilution, Modified Hogde test, E-test Performed molecular techniques (PCR and RT-PCR) to identify antimicrobial genes associated to resistance o IMP, KPC, NDM, OXA, and VIM Supervised and trained junior scientists on all procedures Determined the use of advanced molecular detection methods for strain characterization o MLVA, MLST, SNP, PFGE Transferred results into laboratory information managements systems (LIMS) Postdoctoral Scholar, Penn State University (2013- 2014) Use recombinant DNA techniques to transform Pythium aphanidermatum, Pseudomonas, and Bacillus isolates Analyzed Pythium species for the production of cell communication molecules Analyzed Pythium population diversity in recycled water reservoirs Ph.D. Research, Penn State University (2007-2013) Characterized bacterial isolates that inhibited, promoted, and attached to three pathogenic Pythium species hyphae o Studied biocontrol effects on sporangia development Studied bacteria changes in the presence of Pythium species using ARISA Evaluated interactions bacterium-Pythium and the effect on growth and disease development on geranium Increased national awareness of the survival of Pythium and the complexity of their interaction with bacteria Supervised daily activities of lab tech and undergraduate student Microbiologist II, Bureau of Laboratory (2005-2007) Analyzed clinical samples for the presence of Plasmodium spp; Cyclospora cayetanensis; and Cryptosporidium spp. Analyzed clinical and food samples for the presence of RSV, Influenza, West Nile virus, Norovirus, Clostridium botulism, C. difficile, C. perfringens, Campylobacter jejuni, Listeria monocytogenes, E. coli 0157:H7, Salmonella spp; Shigella spp., Bacillus cereus, and Yersinia enterocolitica o Subtyped Influenza virus (A or B) using cell line culture Experience working with select agents (BSL-3) such as Bacillus anthracis, Brucella spp., Burkholderia spp., Francisella tularensis, Yersinia pestis, and ricin toxin Experience analyzing clinical samples for the presence of Mycobacteria spp. Supervised and trained undergraduate students o Developed teaching and training sessions, supervised research, assessed learning and reported to the director Implemented new analytical procedures o Initiated the use of Multiplex (Luminex) and DNA sequencing for food samples Food Microbiologist / QC tech, Berks Packing Comp. (2004-2005) Analyzed environmental samples to establish quality control efficacy Analyzed ready to eat, environmental, and raw samples for the presence of Listeria monocytogenes and Salmonella typhi Inspected critical control points in accordance to regulations QC technician, Wilbur Chocolate (2004) Analyzed raw material using nuclear magnetic resonance, fatty content, and color test o Prepared samples for HPLC and GC/MS tests Transferred data into ACCESS database Laboratory tech/ Microbiologist, MJ Reider Assoc. Inc. (2000-2003) Performed chemical tests to raw and drinking water Analyzed drinking water for the presence of coliform and fungi Analyzed dairy samples for the presence of antibiotics, bacteria, and fungi Undergraduate researcher, Interamerican University of PR, (1998-1999) Analyzed environmental samples from contaminated and non-contaminated mangroves for the presence of oomycetes o Studied the impact of contaminants in oomycetes population TEACHING AND SUPERVISING EXPERIENCE Guest speaker, Fundamental of Phytopathology, Pennsylvania State University 2013 A lecture aimed to introduce undergraduate and graduate students to genetic fingerprinting and Next-Generation sequencing methods Teacher assistant, Phytobacteriology, Pennsylvania State University 2012 Responsibilities included helping the professor to arranged laboratory sessions, writing handouts, grading assignments, and giving lectures (Topic: Quorum sensing in Bacteria) Teacher assistant, Horticulture Crop Disease, Pennsylvania State University Responsibilities included helping students with online course modules information, deadlines, grading assignments, quizzes, and exams 2012 Supervisor, Virology and Molecular biology, Bureau of Laboratories, 2005-2007 Responsibilities included developing and teaching training sessions, supervising undergraduate students conducting research in the laboratory, assessing their learning and reporting to director Teacher, Science instructor, 10th and Penn Elem. School 2006 Participated in extracurricular activities for K-6 students. Responsibilities included full development of the course including objectives, laboratory sessions, and writing handouts. The main objective was to encourage curiosity for science and pursuing a college degree Teacher assistant, Biology laboratory, Interamerican University of PR 2003 Responsibilities included setting up laboratory materials and helping students during sessions PRESENTATIONS 1. Pest and fungal disease control of mushrooms. Cooperative Extension, Penn State University. Kennett Square, PA. February 2014. 2. Effect of microbes-Pythium spp. interaction and outcomes. Environmental Microbiology Symposium. Department of Plant Pathology, Penn State University, University Park, PA. September, 2013. 3. Bacterial community changes in the presence of three pathogenic Pythium. Environmental Chemistry and Microbiology Symposium. March 2013. 4. Influence of Pythium aphanidermatum, P. irregulare and P. cryptoirregulare on bacterial community in recycled irrigation water. Department of Plant Pathology, The Pennsylvania State University, University Park, PA. March 2012. 5. Influence of Pythium aphanidermatum, P. irregulare, and P. cryptoirregulare on the bacterial community in recycled irrigated water. Northeast APS Division Meeting, New Brunswick, N.J. October 2011. 6. Effects of microbial communities in recycled irrigation water on the development of three Pythium species. Northeast APS Division Meeting, Northampton, MA. October 2010. 7. A plant pathology adventure in Costa Rica. Department of Plant Pathology, The Pennsylvania State University, University Park, PA. Fall 2009. 8. Ratoon stunting disease and two rusts: Just some of the pathogens affecting sugarcane. Department of Plant Pathology, The Pennsylvania State University, University Park, PA. Spring 2008. PUBLICATIONS Peer Reviewed Burgos-Garay, M.L., Hong, C.X., Moorman, G.W. 2014. Interactions of heterotrophic bacteria from recycled greenhouse irrigation water with plant pathogenic Pythium. HORTSCIENCE 49(7):961–967. Manuscript in Preparation Burgos-Garay, Moorman, G.W. 2015. Detection of community shifted by the presence of Pythium in recycled water reservoir. TECHNICAL EXPERIENCE Molecular techniques: DNA/RNA isolation, PCR, RT-PCR, q-PCR, DNA sequencing, DNA transformation, cloning, western blot, fingerprint technique (ARISA). Medical microbiology (BSL-3), food and environmental microbiology, Biolog, Robotic DNA/RNA extraction instruments (MagNaPure and Maxwell). Immunology: ELISA, neutralization mouse assay, and protein extraction (SDSPAGE and membrane blotting). Microscopy: Scanning electron and fluorescence. Computer skills: ACCESS, Excel, Power point, Word, Lync, Outlook, LIMS. Technical programs: BIOMIC, BLAST, Galaxy, Sequencher, MEGA, CIPRES, XLStat, SAS, Minitab, R program, fragment analysis software (GeneMapper, GeneMarker, Peak Scanner), MALDI-TOF, and VITEK2. Knowledge of microarray, RNA-Seq, Next-Gen sequence, DNAStar, PFGE, MLVA, SNP, and MLST. Bilingual (English and Spanish). OTHER PROFESSIONAL EXPERIENCES FastStart Mentoring Program at Pennsylvania State University 2013-2014 Undergraduate research proposal reviewer at Pennsylvania State University 2012 Judge- 24th Annual Graduate Exhibition at Pennsylvania State University 2012 Curriculum and Instruction Committee Graduate Student Representative 2012 Judge- 77th Annual Pennsylvania Junior Academy of Science at Pennsylvania State University 2011 Graduate student advisor council (GSAC) at Pennsylvania State University 2011 Chair for Committee for Diversity and Equality, APS 2009 Vice-president of the Plant Pathology Associate at Pennsylvania State University 2008 PROFESSIONAL AFFILIATIONS Member of American Phytopathology (APS) Member of Committee of Biocontrol Member of Committee of Genetics Member of Committee of Bacteriology Member of Committee of Tropical Plant Pathology Member of Committee of Soil Microbiology American Society for Microbiology (ASM) Botanical Society of America (BSA) 2007-2014 2009-2013 2007-2014 2007-2013 2007-2010 2009-2013 2005-2014 2007-2014 VOLUNTER EXPERIENCES: Mentor to minority and international students (ASM) and as science teacher to K-6 students to promote science interest and pursue college degree.